##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553352_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	908603
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.766055141794602	31.0	31.0	34.0	30.0	34.0
2	31.96149033186111	33.0	31.0	34.0	30.0	34.0
3	32.13630045245283	34.0	31.0	34.0	30.0	34.0
4	35.84172075152735	37.0	35.0	37.0	35.0	37.0
5	35.586941711616625	37.0	35.0	37.0	33.0	37.0
6	34.24040202376616	37.0	35.0	37.0	32.0	37.0
7	35.02446613097249	37.0	35.0	37.0	32.0	37.0
8	35.70379472662978	37.0	35.0	37.0	33.0	37.0
9	37.38470156933226	39.0	37.0	39.0	35.0	39.0
10	36.84091401855376	39.0	37.0	39.0	32.0	39.0
11	36.851635973026724	39.0	37.0	39.0	32.0	39.0
12	36.74858326463813	39.0	35.0	39.0	32.0	39.0
13	36.70576588455024	39.0	35.0	39.0	32.0	39.0
14	37.91260759649704	40.0	37.0	41.0	33.0	41.0
15	37.93158398112267	40.0	37.0	41.0	33.0	41.0
16	37.831288252405066	40.0	37.0	41.0	33.0	41.0
17	37.81270037629196	39.0	37.0	41.0	33.0	41.0
18	37.728036337102125	39.0	37.0	41.0	33.0	41.0
19	37.72142508884518	39.0	36.0	41.0	33.0	41.0
20	37.615248904086826	39.0	36.0	41.0	33.0	41.0
21	37.57939826304778	39.0	36.0	41.0	33.0	41.0
22	37.54329118437865	39.0	36.0	41.0	32.0	41.0
23	37.41891453142902	39.0	36.0	41.0	32.0	41.0
24	37.22510271262587	39.0	35.0	41.0	32.0	41.0
25	37.25444886270461	39.0	35.0	41.0	32.0	41.0
26	37.18106037510332	39.0	35.0	41.0	32.0	41.0
27	37.14476179365465	39.0	35.0	41.0	32.0	41.0
28	37.017980350053875	39.0	35.0	41.0	31.0	41.0
29	36.86647633785052	39.0	35.0	40.0	31.0	41.0
30	36.88877980812302	39.0	35.0	40.0	31.0	41.0
31	36.832369032459724	39.0	35.0	40.0	31.0	41.0
32	36.75787335062728	39.0	35.0	40.0	31.0	41.0
33	36.68158590715637	39.0	35.0	40.0	31.0	41.0
34	36.5996810488189	39.0	35.0	40.0	31.0	41.0
35	36.42752005001084	39.0	35.0	40.0	30.0	41.0
36	36.358104694789695	38.0	35.0	40.0	30.0	41.0
37	36.31699653203874	38.0	35.0	40.0	30.0	41.0
38	36.22450949424556	38.0	35.0	40.0	30.0	41.0
39	36.18289505977858	38.0	35.0	40.0	30.0	41.0
40	36.02465653316135	38.0	35.0	40.0	30.0	41.0
41	35.88920463612821	38.0	35.0	40.0	29.0	41.0
42	35.773184768265125	38.0	35.0	40.0	29.0	41.0
43	35.69788675582185	38.0	34.0	40.0	29.0	41.0
44	35.57905928111617	38.0	34.0	40.0	28.0	41.0
45	35.4477489068383	38.0	34.0	40.0	28.0	41.0
46	35.27632530379054	37.0	34.0	40.0	27.0	41.0
47	35.17726773959584	37.0	34.0	40.0	27.0	41.0
48	35.04579007553354	37.0	34.0	40.0	27.0	41.0
49	34.834599929782314	37.0	33.0	40.0	26.0	41.0
50	34.74075806485341	37.0	33.0	40.0	26.0	41.0
51	33.47644790959308	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	9.0
11	18.0
12	17.0
13	22.0
14	35.0
15	52.0
16	122.0
17	253.0
18	429.0
19	804.0
20	1318.0
21	2012.0
22	2855.0
23	3771.0
24	4796.0
25	5816.0
26	7168.0
27	8784.0
28	11089.0
29	14385.0
30	18600.0
31	23927.0
32	31247.0
33	42780.0
34	63762.0
35	81000.0
36	92951.0
37	128094.0
38	178569.0
39	183890.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.072899825336258	33.395773511643704	20.572241121810077	24.959085541209966
2	22.640361081792598	32.28175561824031	20.429824686909466	24.64805861305763
3	22.406265442663077	32.7983728867283	19.59161481967372	25.2037468509349
4	21.19407485997735	31.02884318013478	19.076758496284956	28.700323463602917
5	19.76638862077277	34.40281399026858	17.36974234071426	28.461055048244393
6	19.46350606370439	39.98732119528551	17.91475484892742	22.63441789208268
7	84.61814455818438	7.6215905076254415	4.124573658682615	3.6356912755075648
8	86.06586154789275	6.947588770893338	3.7971479292936516	3.189401751920255
9	78.34400722867963	10.711388802370232	6.2624710682223155	4.6821329007278205
10	37.429218261440916	33.895991978895076	13.419942483130695	15.254847276533315
11	27.90657746012285	31.164435952775854	22.2807981043426	18.648188482758695
12	27.202639656703752	27.08047409044434	25.79311316383503	19.923773089016876
13	25.325802358125603	27.052519086993993	25.361241378247705	22.2604371766327
14	21.48496097855719	29.119978692564295	26.143211061376643	23.251849267501868
15	21.06376492263398	29.847028900410848	27.075961668627553	22.01324450832762
16	23.01577256513571	29.369592660380828	25.510151298201745	22.104483476281718
17	22.56243926115146	29.833711753097887	25.75360195817095	21.8502470275797
18	23.246236254998056	30.11865468196781	24.33207902681369	22.303030036220438
19	24.71299346359191	29.92803237497565	24.638703592217944	20.720270569214495
20	25.422874456720923	28.934309043663735	24.813147216110888	20.829669283504458
21	25.15862263276701	28.078929961710454	24.944227566935172	21.818219838587368
22	24.589507188508072	27.912410590764065	25.023029860125927	22.475052360601936
23	23.959529079256836	28.20197600051948	25.426506405988093	22.41198851423559
24	20.92773191371809	29.014432045678916	26.72157146740656	23.336264573196434
25	23.456889312494013	30.317421360043934	24.347597355500696	21.878091971961354
26	22.61317649182316	30.606216356318438	24.525232692386005	22.2553744594724
27	22.084342666709222	29.73135681920487	25.17656226096546	23.00773825312045
28	22.99056903840291	31.043260918134763	26.199121068277343	19.76704897518498
29	22.628254584235357	29.64837228140343	25.01961802899616	22.70375510536505
30	22.316897478876914	29.543485988930257	25.516094487911662	22.623522044281167
31	24.06144377687505	30.285944466395115	23.468005278432933	22.184606478296903
32	23.437959152677244	28.896778901236292	25.26868170146918	22.396580244617287
33	22.58412089768579	30.639013958791683	23.9423598645393	22.83450527898323
34	22.91649928516635	30.231245109250132	25.42133362975909	21.430921975824425
35	23.76109257838682	29.42099024546474	25.084993115805254	21.732924060343187
36	23.886669975775998	31.58629236311128	23.967563391272094	20.559474269840624
37	22.662152777395626	30.819400772394545	25.000577810110684	21.51786864009914
38	23.636725830753367	29.158279248472656	25.93189764946847	21.27309727130551
39	23.500252585562674	28.390287067068897	25.250411896064616	22.859048451303813
40	23.89030192504317	28.964135051282025	25.47273121484301	21.6728318088318
41	21.617472097274607	28.756453588641023	26.89755591826133	22.728518395823038
42	21.727861343182887	29.45543873396852	25.846381753086884	22.97031816976171
43	21.90747774330483	29.080357427831515	26.82062462923851	22.19154019962514
44	23.445553228417694	27.479988509833227	25.60161038429325	23.47284787745583
45	22.815905296372563	27.866295840977852	27.05912263111612	22.258676231533464
46	21.17085239648119	29.05471366482391	27.469973134581334	22.304460804113567
47	22.601620289609432	28.52125735882448	26.7947607480935	22.082361603472584
48	22.90681408712056	27.576290194947628	27.703958714642145	21.812937003289665
49	21.267264140664295	29.274501625022147	26.564407117299854	22.8938271170137
50	22.175801752800727	27.092360469864175	27.80092075416876	22.930917023166334
51	22.60789365652546	27.067707238474892	26.6782081943379	23.64619091066175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13796.0
1	10465.5
2	7135.0
3	4568.5
4	2002.0
5	1784.5
6	1567.0
7	1694.0
8	1821.0
9	1746.5
10	1672.0
11	1984.0
12	2296.0
13	2482.5
14	2669.0
15	2313.5
16	1958.0
17	2150.0
18	2342.0
19	2718.0
20	3094.0
21	3674.5
22	4255.0
23	5011.5
24	5768.0
25	6279.0
26	8526.0
27	10262.0
28	13001.5
29	15741.0
30	17033.0
31	18325.0
32	21076.5
33	23828.0
34	26396.0
35	28964.0
36	30848.0
37	32732.0
38	34647.0
39	36562.0
40	37786.5
41	39011.0
42	41262.5
43	43514.0
44	45322.0
45	47130.0
46	52243.0
47	57356.0
48	62246.5
49	67137.0
50	62726.5
51	58316.0
52	55345.0
53	52374.0
54	50636.0
55	48898.0
56	48249.5
57	47601.0
58	44588.0
59	41575.0
60	41165.0
61	40755.0
62	37505.0
63	34255.0
64	31981.5
65	29708.0
66	25971.5
67	22235.0
68	19671.5
69	17108.0
70	15001.0
71	12894.0
72	11056.0
73	9218.0
74	7958.5
75	5505.5
76	4312.0
77	3249.5
78	2187.0
79	1780.5
80	1374.0
81	1043.0
82	712.0
83	474.0
84	236.0
85	176.5
86	117.0
87	151.5
88	186.0
89	113.0
90	40.0
91	26.0
92	12.0
93	12.0
94	12.0
95	11.0
96	10.0
97	21.0
98	32.0
99	21.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	908603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.75017567252752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.56308851581521	16.870108009227827
2	8.635223564018297	3.7563525897789427
3	3.043456511251384	1.98587141314246
4	1.5302192435852469	1.331301494618452
5	0.9265399881247542	1.0076203754667483
6	0.6575893977337748	0.8581610952660702
7	0.48437647345365004	0.7374691372479376
8	0.4019377957010766	0.6993774132741514
9	0.3184772523295354	0.6234242567284143
>10	4.1463460761974416	22.261688564828532
>50	1.5858888862050933	24.726258521832406
>100	0.7001144527066073	22.23242680950491
>500	0.003630223088108334	0.5415390135637722
>1k	0.0025930164915059528	1.0282586227134654
>5k	0.0	0.0
>10k+	5.186032983011906E-4	1.3401426828059109
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11881	1.3076117952505109	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2473	0.27217607690047246	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2240	0.2465323138928663	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	1761	0.1938140199845257	Illumina Single End Adapter 1 (95% over 21bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1415	0.15573358221357403	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1227	0.1350424772975656	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	999	0.10994900963347029	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	912	0.10037387065638127	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2011813740434492E-4	0.0	0.0	0.2381678246715012	0.0
2	2.2011813740434492E-4	0.0	0.0	0.31939141737370447	0.0
3	2.2011813740434492E-4	0.0	0.0	0.41327180297665755	0.0
4	2.2011813740434492E-4	0.0	0.0	0.8656145753425863	0.0
5	2.2011813740434492E-4	0.0	0.0	0.9384736788234246	0.0
6	2.2011813740434492E-4	0.0	0.0	1.3914768056015663	0.0
7	2.2011813740434492E-4	0.0	0.0	1.7050350923340556	0.0
8	2.2011813740434492E-4	0.0	0.0	1.96312360844065	0.0
9	2.2011813740434492E-4	0.0	0.0	2.381788305783714	0.0
10	2.2011813740434492E-4	0.0	0.0	3.072849198164655	0.0
11	2.2011813740434492E-4	0.0	0.0	3.6692592914617275	0.0
12	2.2011813740434492E-4	0.0	0.0	3.9756637387285756	0.0
13	2.2011813740434492E-4	0.0	0.0	4.105753557934544	0.0
14	2.2011813740434492E-4	0.0	0.0	4.254223241613774	0.0
15	2.2011813740434492E-4	0.0	0.0	4.390146191460957	0.0
16	2.2011813740434492E-4	0.0	0.0	4.641411045308017	0.0
17	2.2011813740434492E-4	0.0	0.0	4.935819054086328	0.0
18	2.2011813740434492E-4	0.0	0.0	5.343477844559175	0.0
19	2.2011813740434492E-4	0.0	0.0	5.525735662329972	0.0
20	2.2011813740434492E-4	0.0	0.0	5.736278660757228	0.0
21	3.3017720610651734E-4	0.0	0.0	5.945500950360058	0.0
22	3.3017720610651734E-4	0.0	0.0	6.19533503631399	0.0
23	3.3017720610651734E-4	0.0	0.0	6.433172683779384	0.0
24	3.3017720610651734E-4	0.0	0.0	6.618181978267736	0.0
25	3.3017720610651734E-4	0.0	0.0	6.780298986466036	0.0
26	3.3017720610651734E-4	0.0	0.0	6.922385244160541	0.0
27	3.3017720610651734E-4	0.0	0.0	7.094407568542037	0.0
28	3.3017720610651734E-4	0.0	0.0	7.26026658507621	0.0
29	3.3017720610651734E-4	0.0	0.0	7.42337412489283	0.0
30	3.3017720610651734E-4	0.0	0.0	7.638759722342981	0.0
31	3.3017720610651734E-4	0.0	0.0	7.7977950766176205	0.0
32	3.3017720610651734E-4	0.0	0.0	7.975320354434225	0.0
33	3.3017720610651734E-4	0.0	0.0	8.141069311899697	0.0
34	3.3017720610651734E-4	0.0	0.0	8.29427153553312	0.0
35	4.4023627480868985E-4	0.0	0.0	8.471796813349725	0.0
36	4.4023627480868985E-4	0.0	0.0	8.647781264204498	0.0
37	4.4023627480868985E-4	0.0	0.0	8.821454474616527	0.0
38	4.4023627480868985E-4	0.0	0.0	9.001180933807174	0.0
39	4.4023627480868985E-4	0.0	0.0	9.188501468738272	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGCA	30	2.1649976E-6	45.000004	22
ATTACCG	60	0.0	45.000004	1
TGATTCG	35	1.2115561E-7	45.000004	1
TTAACGG	70	0.0	45.000004	2
TCCGTAT	65	0.0	45.000004	23
CGTCGAT	30	2.1649976E-6	45.000004	27
TATCCCG	30	2.1649976E-6	45.000004	1
CCTTACG	40	6.8121153E-9	45.0	1
TCGTTAA	25	3.890158E-5	45.0	38
ACACGTC	20	7.032506E-4	45.0	27
CGTATTT	25	3.890158E-5	45.0	9
CAACGAA	25	3.890158E-5	45.0	14
CTATCGA	20	7.032506E-4	45.0	1
TAGCGGA	25	3.890158E-5	45.0	44
TAGCGCA	20	7.032506E-4	45.0	23
ATAGCGC	25	3.890158E-5	45.0	36
CACGATA	25	3.890158E-5	45.0	35
TAACGTA	20	7.032506E-4	45.0	34
CACTGCG	25	3.890158E-5	45.0	1
TACGGAT	20	7.032506E-4	45.0	31
>>END_MODULE