##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553351_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	902518
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73834538480119	31.0	31.0	34.0	30.0	34.0
2	31.949796015148728	33.0	31.0	34.0	30.0	34.0
3	32.1250479214819	34.0	31.0	34.0	30.0	34.0
4	35.83595784239206	37.0	35.0	37.0	35.0	37.0
5	35.601264462315434	37.0	35.0	37.0	33.0	37.0
6	32.808817109464854	37.0	35.0	37.0	26.0	37.0
7	34.311405423492936	37.0	35.0	37.0	28.0	37.0
8	35.41595181481145	37.0	35.0	37.0	32.0	37.0
9	37.26390941787311	39.0	37.0	39.0	34.0	39.0
10	36.800182378634	39.0	37.0	39.0	32.0	39.0
11	36.8430757059693	39.0	37.0	39.0	33.0	39.0
12	36.757707879510434	39.0	35.0	39.0	32.0	39.0
13	36.73931600256172	39.0	37.0	39.0	33.0	39.0
14	37.91497787301749	40.0	37.0	41.0	33.0	41.0
15	37.97392960583612	40.0	37.0	41.0	33.0	41.0
16	37.903808012693375	40.0	37.0	41.0	33.0	41.0
17	37.89328633888742	40.0	37.0	41.0	33.0	41.0
18	37.8070653438491	39.0	37.0	41.0	33.0	41.0
19	37.80339450293512	40.0	36.0	41.0	33.0	41.0
20	37.668561735056805	39.0	36.0	41.0	33.0	41.0
21	37.6372371520568	39.0	36.0	41.0	33.0	41.0
22	37.56379374150986	39.0	36.0	41.0	32.0	41.0
23	37.45731719478171	39.0	36.0	41.0	32.0	41.0
24	37.30781325137006	39.0	36.0	41.0	32.0	41.0
25	37.31712940905334	39.0	36.0	41.0	32.0	41.0
26	37.26075601816252	39.0	35.0	41.0	32.0	41.0
27	37.19756281869171	39.0	35.0	41.0	32.0	41.0
28	37.092672943919126	39.0	35.0	41.0	32.0	41.0
29	36.91332582840453	39.0	35.0	40.0	31.0	41.0
30	36.943270937532546	39.0	35.0	40.0	31.0	41.0
31	36.58720934097713	39.0	35.0	40.0	30.0	41.0
32	36.685488821275584	39.0	35.0	40.0	31.0	41.0
33	36.6699622611405	39.0	35.0	40.0	31.0	41.0
34	36.61765748716369	39.0	35.0	40.0	31.0	41.0
35	36.52177463496573	39.0	35.0	40.0	30.0	41.0
36	36.431465078812835	39.0	35.0	40.0	30.0	41.0
37	36.36390520743077	38.0	35.0	40.0	30.0	41.0
38	36.28237331554606	38.0	35.0	40.0	30.0	41.0
39	36.214662754648664	38.0	35.0	40.0	30.0	41.0
40	36.081971772308144	38.0	35.0	40.0	30.0	41.0
41	36.00500710235142	38.0	35.0	40.0	30.0	41.0
42	35.87567782581622	38.0	35.0	40.0	29.0	41.0
43	35.82208554289222	38.0	35.0	40.0	29.0	41.0
44	35.65033384375713	38.0	34.0	40.0	29.0	41.0
45	35.515511047979096	38.0	34.0	40.0	28.0	41.0
46	35.2381038383722	37.0	34.0	40.0	27.0	41.0
47	35.20989055065938	37.0	34.0	40.0	27.0	41.0
48	35.173421471926325	37.0	34.0	40.0	27.0	41.0
49	35.01319973673655	37.0	34.0	40.0	27.0	41.0
50	34.89414726354488	37.0	34.0	40.0	27.0	41.0
51	33.69663208933229	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	9.0
11	13.0
12	26.0
13	24.0
14	37.0
15	86.0
16	110.0
17	236.0
18	424.0
19	748.0
20	1312.0
21	1973.0
22	2745.0
23	3705.0
24	4590.0
25	5878.0
26	7017.0
27	8535.0
28	11099.0
29	13776.0
30	18069.0
31	23703.0
32	31324.0
33	43012.0
34	63480.0
35	81527.0
36	95239.0
37	130597.0
38	175326.0
39	177867.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.928003652004726	33.493847214127584	20.55116906255609	25.0269800713116
2	22.66802434965286	32.09021869923924	20.39626910488212	24.84548784622578
3	22.413070985841834	32.726327895953325	19.66054970648785	25.200051411716995
4	21.126559248679804	31.01012943786163	19.120948280255906	28.74236303320266
5	19.83406425135011	34.197877493856076	17.51854256646405	28.44951568832976
6	18.742673276322467	42.43261630238953	17.150572066152698	21.6741383551353
7	84.67011184264469	7.612812154439025	4.129335924602058	3.5877400783142277
8	85.96183123217487	6.985234643519575	3.7889549017304915	3.263979222575062
9	78.45849057858126	10.655743154153157	6.254279693036594	4.63148657422899
10	37.27648645234776	34.00896159411779	13.49768093267946	15.216871020854986
11	27.829694255405435	31.149738841773793	22.297727025942972	18.722839876877803
12	27.202005943371766	27.143502955065717	25.81942964018446	19.835061461378057
13	25.411459937641133	26.957467884297042	25.34774929696693	22.28332288109489
14	21.397246370709507	29.150775940202855	26.218424452476295	23.23355323661135
15	21.12811046427883	29.886827741939776	27.091758834726843	21.893302959054555
16	23.06214391291919	29.344788691195077	25.557274203949394	22.035793191936335
17	22.643537303411122	29.735141016578066	25.718933029590545	21.902388650420267
18	23.228899589814276	30.181226302411694	24.437961348139318	22.151912759634712
19	24.841388204999788	29.823671106836652	24.625215231164365	20.709725456999195
20	25.405255075245037	29.055930186433955	24.714077724765602	20.82473701355541
21	25.133681544301613	28.108913063229764	25.015678357661564	21.741727034807063
22	24.701224795516545	27.90681183089977	25.034625348192503	22.357338025391183
23	24.03320487790825	28.06714104317033	25.471846544888855	22.427807534032564
24	20.90562182693309	29.035875184760858	26.803565136651013	23.254937851655036
25	23.494822263932686	30.273745232782062	24.3596249603886	21.87180754289665
26	22.597998045468344	30.60758898991488	24.639397773784015	22.155015190832756
27	22.10814631951939	29.710653970336327	25.268637301416703	22.91256240872758
28	22.924085724606048	31.153727681885567	26.1755444212747	19.746642172233685
29	22.50957875632397	29.55497840486284	25.18442845461254	22.751014384200648
30	22.389691950742257	29.402294469473183	25.658214019000177	22.549799560784383
31	24.03686131467738	30.245601749771193	23.483188146940005	22.23434878861142
32	23.46811919540663	28.874216359119707	25.377665597805247	22.279998847668413
33	22.59035276858744	30.540221912471548	23.947444815505065	22.921980503435943
34	22.918988873352113	30.18455033583818	25.35550537496205	21.540955415847662
35	23.739360323007407	29.39631120930552	25.18963610698069	21.67469236070638
36	23.953760479015376	31.58075517607405	24.0757525057672	20.389731839143373
37	22.63234639087531	30.74597958157067	25.103100436777993	21.51857359077603
38	23.656148686231187	28.9953219769578	26.030062558309087	21.318466778501925
39	23.408286593729986	28.515774754630936	25.366253083041002	22.70968556859808
40	23.94788801996193	28.975931781969997	25.419105214522038	21.657074983546035
41	21.58871069607476	28.77759778752335	26.899629702676293	22.7340618137256
42	21.825049472697497	29.42135226111834	25.855107598962014	22.89849066722215
43	21.91003392730117	29.13016693295868	26.82229052495352	22.13750861478663
44	23.452496238302174	27.542608568471767	25.561927850746468	23.442967342479594
45	22.814614223760636	27.90237978633113	27.0519812347233	22.23102475518494
46	21.099080572354236	29.226563902326603	27.4694798330892	22.20487569222996
47	22.566973733487863	28.573391334023256	26.85852248930215	22.00111244318673
48	22.89749345719421	27.60676241360283	27.70892104090999	21.786823088292977
49	21.32600125426861	29.22523428895601	26.572655614624864	22.876108842150515
50	22.08620769890462	27.236243487664513	27.780498560693527	22.89705025273734
51	22.602208487808554	27.158239503256443	26.7418489160327	23.497703092902302
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13511.0
1	10274.0
2	7037.0
3	4566.5
4	2096.0
5	1843.5
6	1591.0
7	1718.0
8	1845.0
9	1763.5
10	1682.0
11	2030.0
12	2378.0
13	2516.0
14	2654.0
15	2299.5
16	1945.0
17	2148.0
18	2351.0
19	2667.0
20	2983.0
21	3645.0
22	4307.0
23	5073.5
24	5840.0
25	6269.5
26	8536.5
27	10374.0
28	13124.0
29	15874.0
30	17025.0
31	18176.0
32	21050.5
33	23925.0
34	26670.5
35	29416.0
36	31074.5
37	32733.0
38	34727.5
39	36722.0
40	37698.0
41	38674.0
42	41085.5
43	43497.0
44	45265.5
45	47034.0
46	51887.0
47	56740.0
48	61469.0
49	66198.0
50	61938.5
51	57679.0
52	54793.5
53	51908.0
54	50091.0
55	48274.0
56	47578.5
57	46883.0
58	44120.0
59	41357.0
60	40848.0
61	40339.0
62	37355.5
63	34372.0
64	31843.0
65	29314.0
66	25622.5
67	21931.0
68	19300.0
69	16669.0
70	14726.5
71	12784.0
72	10979.5
73	9175.0
74	7869.0
75	5369.5
76	4176.0
77	3166.5
78	2157.0
79	1777.5
80	1398.0
81	1031.0
82	664.0
83	415.5
84	167.0
85	147.0
86	127.0
87	149.5
88	172.0
89	103.5
90	35.0
91	26.0
92	17.0
93	19.0
94	21.0
95	18.0
96	15.0
97	25.0
98	35.0
99	19.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	902518.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.03957628963235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.61214948125617	16.664597369152307
2	8.920552316933279	3.9321038666941517
3	3.39320746939771	2.243545646650235
4	1.8190800217623624	1.6036701166631064
5	1.1903370234714599	1.3117261819586568
6	0.8799356839090747	1.1636045781290323
7	0.663070121479224	1.0229649169402004
8	0.4768893844775152	0.8408351976726408
9	0.41474062451242794	0.822663687091628
>10	4.437269970362396	23.11307572667002
>50	1.5645144010886884	24.485722532251227
>100	0.6221341490638287	20.035530350947067
>500	0.0030596761429368623	0.4676545526414766
>1k	0.002549730119114052	0.9956625046505266
>5k	0.0	0.0
>10k+	5.099460238228104E-4	1.296642771887699
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11537	1.2783124547100446	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2399	0.26581187300419495	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2190	0.24265444013305	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	1626	0.1801626117152234	Illumina Single End Adapter 1 (95% over 21bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1396	0.15467835544554234	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1248	0.1382797905415737	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	944	0.1045962518199083	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	910	0.10082901393656414	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.218721399462393	0.0
2	0.0	0.0	0.0	0.2951741682714361	0.0
3	0.0	0.0	0.0	0.3844798663295358	0.0
4	0.0	0.0	0.0	0.8100669460332093	0.0
5	0.0	0.0	0.0	0.875439603420652	0.0
6	0.0	0.0	0.0	1.3003618764390295	0.0
7	0.0	0.0	0.0	1.60484333830461	0.0
8	0.0	0.0	0.0	1.8596859010014204	0.0
9	0.0	0.0	0.0	2.268320410230045	0.0
10	0.0	0.0	0.0	2.9341243055540165	0.0
11	0.0	0.0	0.0	3.5375471735743775	0.0
12	0.0	0.0	0.0	3.8257408716502055	0.0
13	0.0	0.0	0.0	3.9458492794603544	0.0
14	0.0	0.0	0.0	4.08379666665928	0.0
15	0.0	0.0	0.0	4.212325959149846	0.0
16	0.0	0.0	0.0	4.457085620452999	0.0
17	0.0	0.0	0.0	4.754808214351404	0.0
18	0.0	0.0	0.0	5.136961257282403	0.0
19	0.0	0.0	0.0	5.30537895089073	0.0
20	1.1080111421600456E-4	0.0	0.0	5.516233471243787	0.0
21	1.1080111421600456E-4	0.0	0.0	5.732295643964996	0.0
22	1.1080111421600456E-4	0.0	0.0	5.968634420587733	0.0
23	1.1080111421600456E-4	0.0	0.0	6.200984357098695	0.0
24	1.1080111421600456E-4	0.0	0.0	6.377158128702142	0.0
25	1.1080111421600456E-4	0.0	0.0	6.5391493576859405	0.0
26	1.1080111421600456E-4	0.0	0.0	6.683190806166746	0.0
27	1.1080111421600456E-4	0.0	0.0	6.84728725632065	0.0
28	2.2160222843200912E-4	0.0	0.0	7.007394866362776	0.0
29	2.2160222843200912E-4	0.0	0.0	7.165175653006367	0.0
30	2.2160222843200912E-4	0.0	0.0	7.375365366674127	0.0
31	2.2160222843200912E-4	0.0	0.0	7.530265324348101	0.0
32	3.324033426480137E-4	0.0	0.0	7.696688597900541	0.0
33	3.324033426480137E-4	0.0	0.0	7.865549495965731	0.0
34	3.324033426480137E-4	0.0	0.0	8.025103100436779	0.0
35	3.324033426480137E-4	0.0	0.0	8.206041319951513	0.0
36	3.324033426480137E-4	0.0	0.0	8.374569814674055	0.0
37	3.324033426480137E-4	0.0	0.0	8.54387391719611	0.0
38	3.324033426480137E-4	0.0	0.0	8.711072798548063	0.0
39	3.324033426480137E-4	0.0	0.0	8.889794995778477	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	30	2.1649812E-6	45.000004	1
AACGCAC	30	2.1649812E-6	45.000004	3
CCGAAAT	30	2.1649812E-6	45.000004	45
GATTCGA	30	2.1649812E-6	45.000004	9
TATACGG	60	0.0	45.000004	2
AATACGA	35	1.2115379E-7	45.000004	20
AACCGCA	20	7.0324814E-4	45.0	28
CGAACAT	20	7.0324814E-4	45.0	22
TACGCTA	20	7.0324814E-4	45.0	18
ATATCGC	20	7.0324814E-4	45.0	18
GTTCGCA	40	6.8121153E-9	45.0	13
TACGAAC	20	7.0324814E-4	45.0	38
TCGTATC	20	7.0324814E-4	45.0	36
GCGATAT	20	7.0324814E-4	45.0	42
CGTAAGA	25	3.8901377E-5	45.0	28
GCTCGAT	20	7.0324814E-4	45.0	10
CAATCGA	40	6.8121153E-9	45.0	41
CGATCAC	20	7.0324814E-4	45.0	41
CGCCCAT	20	7.0324814E-4	45.0	16
CGATATG	55	1.8189894E-12	44.999996	1
>>END_MODULE