FastQCFastQC Report
Sat 18 Jun 2016
SRR3553347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684224
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA151862.2194486016275374No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33090.4836135534561781No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30580.4469296604620709No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT20940.3060401272098026Illumina Single End Adapter 1 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15440.22565709475259565No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8770.12817439902721917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA302.1642081E-645.00000445
ACTATGC207.031282E-445.00000440
CTCACGA207.031282E-445.00000424
TCGTTAC700.045.0000049
TACCCGA207.031282E-445.00000418
CTCGTAA207.031282E-445.00000434
CGTATAG351.2109558E-745.0000041
GTCGACA207.031282E-445.00000432
CGAAACG207.031282E-445.0000041
CTATCGC406.8084773E-945.00000439
TCCGACT207.031282E-445.00000424
GACCGTT207.031282E-445.00000431
GACCGTC207.031282E-445.00000427
GACGTAC207.031282E-445.00000410
CGTTGCA207.031282E-445.00000434
CGCATAC207.031282E-445.00000423
GATCCAT207.031282E-445.00000410
ATAGCGG351.2109558E-745.0000042
TGAACGT406.8084773E-945.00000436
CGACCGT207.031282E-445.00000423