##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553347_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	684224
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62563429520157	31.0	31.0	34.0	30.0	34.0
2	31.816712070900756	33.0	31.0	34.0	30.0	34.0
3	31.978759879805445	33.0	31.0	34.0	30.0	34.0
4	35.734312155083714	37.0	35.0	37.0	35.0	37.0
5	35.48471114722664	37.0	35.0	37.0	33.0	37.0
6	32.596724172200915	37.0	35.0	37.0	26.0	37.0
7	34.14808600692171	37.0	35.0	37.0	26.0	37.0
8	35.2696412286035	37.0	35.0	37.0	32.0	37.0
9	37.10036625432607	39.0	37.0	39.0	34.0	39.0
10	36.6318062505846	38.0	35.0	39.0	32.0	39.0
11	36.718405083715275	39.0	37.0	39.0	32.0	39.0
12	36.6984613226078	39.0	35.0	39.0	32.0	39.0
13	36.692002911327286	39.0	35.0	39.0	32.0	39.0
14	37.830118499204936	40.0	37.0	41.0	33.0	41.0
15	37.87761464081938	40.0	37.0	41.0	33.0	41.0
16	37.81737267327659	40.0	37.0	41.0	33.0	41.0
17	37.80280872930502	39.0	37.0	41.0	33.0	41.0
18	37.640535847909455	39.0	36.0	41.0	33.0	41.0
19	37.59493236133196	39.0	36.0	41.0	33.0	41.0
20	37.479348868206905	39.0	36.0	41.0	32.0	41.0
21	37.36877396875877	39.0	35.0	41.0	32.0	41.0
22	37.311668400991486	39.0	35.0	41.0	32.0	41.0
23	37.16926328219998	39.0	35.0	40.0	32.0	41.0
24	37.10090555139837	39.0	35.0	40.0	31.0	41.0
25	37.0438993662894	39.0	35.0	40.0	31.0	41.0
26	37.00856736975026	39.0	35.0	40.0	31.0	41.0
27	36.882192089140396	39.0	35.0	40.0	31.0	41.0
28	36.71884938265831	39.0	35.0	40.0	31.0	41.0
29	36.43513527733608	39.0	35.0	40.0	30.0	41.0
30	36.47633669675428	39.0	35.0	40.0	30.0	41.0
31	36.07736501496586	38.0	35.0	40.0	29.0	41.0
32	36.1859040314283	38.0	35.0	40.0	30.0	41.0
33	36.14883283836872	38.0	35.0	40.0	30.0	41.0
34	36.11621778832663	38.0	35.0	40.0	30.0	41.0
35	36.08994715180993	38.0	35.0	40.0	30.0	41.0
36	35.94557630249743	38.0	35.0	40.0	29.0	41.0
37	35.89015293237303	38.0	35.0	40.0	29.0	41.0
38	35.73081914694603	38.0	35.0	40.0	29.0	41.0
39	35.63558279160041	38.0	35.0	40.0	28.0	41.0
40	35.5134590426527	38.0	34.0	40.0	27.0	41.0
41	35.434020145449445	38.0	34.0	40.0	27.0	41.0
42	35.28748480029932	38.0	34.0	40.0	27.0	41.0
43	35.13162063885511	38.0	34.0	40.0	26.0	41.0
44	34.97179724768497	38.0	34.0	40.0	26.0	41.0
45	34.808529078196614	37.0	33.0	40.0	25.0	41.0
46	34.552487197175196	37.0	33.0	40.0	24.0	41.0
47	34.583954962117666	37.0	33.0	40.0	24.0	41.0
48	34.523050930689365	37.0	33.0	40.0	24.0	41.0
49	34.32094460293705	37.0	33.0	40.0	24.0	41.0
50	34.13027020390983	36.0	33.0	40.0	23.0	41.0
51	33.0506135417641	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	6.0
12	8.0
13	11.0
14	20.0
15	41.0
16	95.0
17	224.0
18	392.0
19	814.0
20	1431.0
21	2307.0
22	3295.0
23	4037.0
24	5080.0
25	5864.0
26	6793.0
27	8053.0
28	10028.0
29	12285.0
30	15435.0
31	19833.0
32	25633.0
33	34297.0
34	49201.0
35	67208.0
36	71284.0
37	93694.0
38	122922.0
39	123900.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.20613717145262	32.66357216350201	22.822496726218315	25.30779393882705
2	23.896267888878494	32.413215555139836	20.437897530633244	23.252619025348423
3	19.593583387896363	34.27517888878496	20.43234379384529	25.69889392947339
4	19.532199981292674	34.425275933027784	19.38853357964643	26.65399050603311
5	18.649301982976336	37.9153610513516	16.14368978580114	27.29164717987092
6	17.588684407445516	46.221120568702645	15.927824805911515	20.262370217940322
7	83.16443153119447	9.334077728930877	3.846839631465719	3.654651108408942
8	84.22271069123562	9.0872287438032	3.436155411093443	3.2539051538677395
9	76.83375619680105	12.93231456365167	6.407404592648022	3.8265246468992613
10	34.38406136002245	40.64370732391732	11.81776728089047	13.154464035169768
11	24.7123748947713	34.32735478439809	24.42197292114863	16.538297399681976
12	27.35025371808063	27.91498105883453	26.887539753063322	17.847225470021513
13	25.630641427368815	28.732695725376484	25.17041202880928	20.466250818445424
14	19.889539098306987	32.25785707604527	25.63604901318866	22.216554812459076
15	17.97496141614442	34.026283790103825	28.370241324478535	19.62851346927322
16	20.54385698250865	32.44756103264428	26.260698250865218	20.747883733981855
17	20.8041518567019	30.987951314189505	25.84972172855673	22.358175100551865
18	23.53439809185296	30.414747217285566	24.3589818538958	21.691872836965672
19	22.266830745486857	33.99310752034421	24.585223552520812	19.154838181648117
20	24.77156603685343	31.66039191843607	23.82436161257132	19.743680432139183
21	24.724066972219624	31.916740716490505	24.729620709007577	18.629571602282294
22	21.05260850247872	32.04842273875222	24.298183051164532	22.600785707604526
23	20.769367926293146	32.72276330558414	24.11344822748106	22.39442054064166
24	20.28268520250678	31.17853217659714	26.025833645122066	22.512948975774016
25	21.348856514825552	34.07889813862127	22.08648045084651	22.48576489570667
26	22.68029183425311	32.56024342905248	22.864295903096064	21.89516883359835
27	20.35035310073894	33.46915629969133	23.637142222430082	22.54334837713965
28	21.186044336357686	34.51998176035918	25.019730380694043	19.274243522589092
29	21.729141333832196	33.62363787297727	24.44521092507717	20.202009868113365
30	22.932986858104947	31.981777897296794	23.345278739126368	21.73995650547189
31	21.767140585539238	34.24507178935553	22.2064703956599	21.781317229445328
32	22.165109671686466	33.158293190534096	23.764732017584887	20.911865120194555
33	21.820631839865307	33.32855906837527	23.726440463941636	21.12436862781779
34	21.31304952764007	33.6030305864746	24.16635487793471	20.91756500795061
35	22.224154662800487	32.06479164717987	24.621030539706297	21.090023150313346
36	20.564025816107005	34.48592858479095	25.03171476007857	19.918330839023476
37	21.87792301936208	34.86519034702086	23.12283696567206	20.134049667945
38	21.85760803479562	31.710521700495743	25.572473342063418	20.859396922645214
39	23.194012487138714	28.643105181928725	25.20870358245253	22.954178748480032
40	25.054075858198484	30.549936862781777	23.33007903844355	21.065908240576185
41	21.962982882798617	29.277838836404452	26.344588906556915	22.414589374240016
42	23.49800650079506	30.72005658965485	23.961451220652886	21.820485688897204
43	21.729872088672717	32.19150453652605	24.544885885324106	21.53373748947713
44	22.673130436816013	30.651657936582172	23.286964502852868	23.388247123748947
45	22.06733467402488	29.859227387522214	25.165735197829953	22.907702740622955
46	20.071058600692172	31.628969460293703	25.418137919745583	22.881834019268542
47	21.752087035824523	31.714467776634553	24.4354188102142	22.098026377326725
48	22.37322865026658	29.25913151248714	26.659251940884854	21.708387896361426
49	22.111326115424188	30.471161490973714	25.524389673557195	21.893122720044897
50	20.968863997755122	29.333083902347767	27.026967776634553	22.67108432326256
51	21.811424328874754	28.116377092881862	26.172715368066594	23.899483210176783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17432.0
1	13346.5
2	9261.0
3	5801.0
4	2341.0
5	1937.5
6	1534.0
7	1645.5
8	1757.0
9	1886.5
10	2016.0
11	2300.5
12	2585.0
13	2752.0
14	2919.0
15	3096.0
16	3273.0
17	3329.0
18	3385.0
19	3560.5
20	3736.0
21	3634.0
22	3532.0
23	3753.0
24	3974.0
25	4423.0
26	5870.0
27	6868.0
28	8089.0
29	9310.0
30	11224.5
31	13139.0
32	15006.0
33	16873.0
34	19593.0
35	22313.0
36	22773.5
37	23234.0
38	24982.5
39	26731.0
40	25820.0
41	24909.0
42	28498.5
43	32088.0
44	37186.0
45	42284.0
46	47488.0
47	52692.0
48	52859.0
49	53026.0
50	52071.0
51	51116.0
52	48106.0
53	45096.0
54	40364.0
55	35632.0
56	32633.5
57	29635.0
58	29381.0
59	29127.0
60	29315.0
61	29503.0
62	25742.5
63	21982.0
64	19713.0
65	17444.0
66	14486.5
67	11529.0
68	10179.0
69	8829.0
70	7859.0
71	6889.0
72	5551.5
73	4214.0
74	3585.5
75	2325.0
76	1693.0
77	1357.0
78	1021.0
79	772.5
80	524.0
81	395.0
82	266.0
83	326.5
84	387.0
85	323.5
86	260.0
87	139.0
88	18.0
89	16.0
90	14.0
91	7.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	684224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.713306320274075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.38350210068009	19.897856936503597
2	9.260216440495077	4.762215638529759
3	3.4910221037778197	2.6929716217586015
4	1.8845200185294737	1.9382896201254773
5	1.1623614049686737	1.4944077430411826
6	0.8104462412103658	1.250355147381412
7	0.62057957118413	1.1170006826973307
8	0.48376957688987193	0.9951452255198927
9	0.3794645025149274	0.8781558291753068
>10	3.043129742014784	16.942734316995757
>50	0.7738652915513909	14.433242095502916
>100	0.7030954544801485	29.66686796001166
>500	0.0011507290580689829	0.203868545968583
>1k	0.0023014581161379657	1.4801921643074547
>5k	0.0	0.0
>10k+	5.753645290344914E-4	2.24669647248106
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15186	2.2194486016275374	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3309	0.4836135534561781	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3058	0.4469296604620709	No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT	2094	0.3060401272098026	Illumina Single End Adapter 1 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1544	0.22565709475259565	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	877	0.12817439902721917	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.37750795061266484	0.0
2	0.0	0.0	0.0	0.48463661023290616	0.0
3	0.0	0.0	0.0	0.6113494995790852	0.0
4	0.0	0.0	0.0	1.2012148068468806	0.0
5	0.0	0.0	0.0	1.3125818445421382	0.0
6	0.0	0.0	0.0	1.9365003273781685	0.0
7	0.0	0.0	0.0	2.438675053783556	0.0
8	0.0	0.0	0.0	3.031463380413432	0.0
9	0.0	0.0	0.0	3.730357309886821	0.0
10	0.0	0.0	0.0	4.854258254606679	0.0
11	0.0	0.0	0.0	5.793716677579273	0.0
12	0.0	0.0	0.0	6.332575296978767	0.0
13	0.0	0.0	0.0	6.626046440931625	0.0
14	0.0	0.0	0.0	6.8968641848283605	0.0
15	0.0	0.0	0.0	7.091098821438593	0.0
16	0.0	0.0	0.0	7.51274436441867	0.0
17	0.0	0.0	0.0	8.058472079319053	0.0
18	0.0	0.0	0.0	8.871509914881676	0.0
19	0.0	0.0	0.0	9.151681320737069	0.0
20	0.0	0.0	0.0	9.473505752502104	0.0
21	0.0	0.0	0.0	9.803222336544756	0.0
22	0.0	0.0	0.0	10.169622813581517	0.0
23	0.0	0.0	0.0	10.536607894490693	0.0
24	0.0	0.0	0.0	10.79295669254513	0.0
25	0.0	0.0	0.0	11.004290992423535	0.0
26	0.0	0.0	0.0	11.220302123281265	0.0
27	0.0	0.0	0.0	11.481035450378823	0.0
28	0.0	0.0	0.0	11.714438546440931	0.0
29	0.0	0.0	0.0	11.939511037321111	0.0
30	0.0	0.0	0.0	12.265719998129267	0.0
31	0.0	0.0	0.0	12.51198437938453	0.0
32	0.0	0.0	0.0	12.744510569638013	0.0
33	0.0	0.0	0.0	12.993697970255354	0.0
34	0.0	0.0	0.0	13.2393777476382	0.0
35	0.0	0.0	0.0	13.489442054064167	0.0
36	0.0	0.0	0.0	13.75543681601347	0.0
37	0.0	0.0	0.0	13.99950893274717	0.0
38	0.0	0.0	0.0	14.254688523056776	0.0
39	0.0	0.0	0.0	14.545967402488074	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAA	30	2.1642081E-6	45.000004	45
ACTATGC	20	7.031282E-4	45.000004	40
CTCACGA	20	7.031282E-4	45.000004	24
TCGTTAC	70	0.0	45.000004	9
TACCCGA	20	7.031282E-4	45.000004	18
CTCGTAA	20	7.031282E-4	45.000004	34
CGTATAG	35	1.2109558E-7	45.000004	1
GTCGACA	20	7.031282E-4	45.000004	32
CGAAACG	20	7.031282E-4	45.000004	1
CTATCGC	40	6.8084773E-9	45.000004	39
TCCGACT	20	7.031282E-4	45.000004	24
GACCGTT	20	7.031282E-4	45.000004	31
GACCGTC	20	7.031282E-4	45.000004	27
GACGTAC	20	7.031282E-4	45.000004	10
CGTTGCA	20	7.031282E-4	45.000004	34
CGCATAC	20	7.031282E-4	45.000004	23
GATCCAT	20	7.031282E-4	45.000004	10
ATAGCGG	35	1.2109558E-7	45.000004	2
TGAACGT	40	6.8084773E-9	45.000004	36
CGACCGT	20	7.031282E-4	45.000004	23
>>END_MODULE