##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335374
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42212276443612	31.0	31.0	34.0	30.0	34.0
2	31.608627979509443	31.0	31.0	34.0	30.0	34.0
3	31.820656938224193	31.0	31.0	34.0	30.0	34.0
4	35.591134077179504	37.0	35.0	37.0	33.0	37.0
5	35.31371543411237	37.0	35.0	37.0	33.0	37.0
6	33.97141698521651	37.0	35.0	37.0	31.0	37.0
7	34.8167896139832	37.0	35.0	37.0	32.0	37.0
8	35.48681770202818	37.0	35.0	37.0	32.0	37.0
9	37.112265709327495	39.0	37.0	39.0	34.0	39.0
10	36.50365263854682	38.0	35.0	39.0	32.0	39.0
11	36.59307519366439	39.0	35.0	39.0	32.0	39.0
12	36.66075187700895	39.0	35.0	39.0	32.0	39.0
13	36.62518263192734	39.0	35.0	39.0	32.0	39.0
14	37.74237120349222	39.0	37.0	41.0	33.0	41.0
15	37.74446438901048	39.0	37.0	41.0	33.0	41.0
16	37.6306153726884	39.0	36.0	41.0	32.0	41.0
17	37.570563013232984	39.0	36.0	40.0	32.0	41.0
18	37.339185506330246	39.0	36.0	40.0	32.0	41.0
19	37.1439557031851	39.0	35.0	40.0	32.0	41.0
20	37.02802244658203	39.0	35.0	40.0	31.0	41.0
21	36.9703375932541	39.0	35.0	40.0	31.0	41.0
22	36.947106812096344	39.0	35.0	40.0	31.0	41.0
23	36.721493019733195	39.0	35.0	40.0	31.0	41.0
24	36.55834680088498	38.0	35.0	40.0	31.0	41.0
25	36.628250848306664	38.0	35.0	40.0	31.0	41.0
26	36.55539785433576	38.0	35.0	40.0	31.0	41.0
27	36.484697680798156	38.0	35.0	40.0	31.0	41.0
28	36.41484432305426	38.0	35.0	40.0	31.0	41.0
29	36.24087436712446	38.0	35.0	40.0	30.0	41.0
30	36.16612200110921	38.0	35.0	40.0	30.0	41.0
31	36.08120784556942	38.0	35.0	40.0	30.0	41.0
32	35.97169726931724	38.0	35.0	40.0	30.0	41.0
33	35.87268840160537	38.0	35.0	40.0	30.0	41.0
34	35.80341648428322	38.0	35.0	40.0	29.0	41.0
35	35.638925498100626	38.0	35.0	40.0	29.0	41.0
36	35.481566847758025	38.0	34.0	40.0	28.0	41.0
37	35.421675502573244	38.0	34.0	40.0	27.0	41.0
38	35.390811452289086	38.0	34.0	40.0	27.0	41.0
39	35.33222909348966	38.0	34.0	40.0	27.0	41.0
40	35.180437362466975	38.0	34.0	40.0	26.0	41.0
41	35.057494021599766	38.0	34.0	40.0	26.0	41.0
42	34.941832103860165	38.0	34.0	40.0	25.0	41.0
43	34.88839027473805	37.0	34.0	40.0	25.0	41.0
44	34.762984011879276	37.0	33.0	40.0	24.0	41.0
45	34.59609868385743	37.0	33.0	40.0	24.0	41.0
46	34.413833511244164	37.0	33.0	40.0	23.0	41.0
47	34.36286056760512	37.0	33.0	40.0	23.0	41.0
48	34.17182011724224	37.0	33.0	40.0	23.0	41.0
49	33.978185548074684	36.0	33.0	40.0	23.0	41.0
50	33.902079469487795	36.0	33.0	40.0	23.0	41.0
51	32.80068818691968	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	4.0
14	8.0
15	27.0
16	51.0
17	104.0
18	222.0
19	531.0
20	803.0
21	1201.0
22	1711.0
23	2148.0
24	2577.0
25	3078.0
26	3468.0
27	4281.0
28	5192.0
29	6497.0
30	7972.0
31	10449.0
32	13370.0
33	17511.0
34	24362.0
35	38933.0
36	36278.0
37	44301.0
38	57704.0
39	52577.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.49554825359151	36.7586634622839	19.48451579430725	24.261272489817337
2	18.43881755890439	39.13541300160418	20.494731255255328	21.931038184236108
3	18.956150446963687	39.04894237478159	19.770167037397055	22.22474014085767
4	17.645375014163292	38.8694412804809	19.68011831567146	23.80506538968435
5	16.27257926971083	41.497253812161944	17.197218627562062	25.03294829056516
6	16.719244783435805	45.612659299766825	18.071466482195998	19.596629434601372
7	76.87447446731112	14.945106060696418	4.6255821858581765	3.554837286134286
8	78.09728840041268	14.338320800062021	3.6058251385020905	3.958565661023216
9	71.1772528580033	17.969490777460386	7.174676629673141	3.6785797348631672
10	24.961982741655582	52.53209849302569	10.28940824273796	12.21651052258076
11	16.794384776398886	35.750833397937825	32.45570616684657	14.999075658816723
12	20.614001085355454	31.736210916767547	30.702141489799452	16.94764650807755
13	20.54482458389738	34.198536559184674	26.878947085939874	18.37769177097807
14	15.793114552708321	36.91490693971507	27.70787240513576	19.584106102440856
15	14.440892853948128	39.50097503086107	27.989051029596805	18.069081085593993
16	16.88055722864623	39.70343556745603	24.67752419686678	18.738483007030958
17	16.764269144298606	38.13950992026812	25.61737045805578	19.478850477377495
18	17.282496556083657	38.564110515424574	24.780394425328144	19.372998503163632
19	16.427033699690494	40.784914751888934	25.433396745126334	17.354654803294235
20	17.675192471688327	39.113049908460404	25.61587958517953	17.59587803467174
21	17.01503396208412	40.01115172911437	25.556542844704715	17.4172714640968
22	17.25834441548838	37.20234723025637	25.680285293433595	19.859023060821652
23	16.886222545575986	39.13272943042693	23.507188989009283	20.473859034987804
24	13.600040551742234	39.80779666879364	26.567951003953794	20.024211775510324
25	16.43269901662025	40.704407616571345	24.406185333388994	18.456708033419407
26	19.233750976521733	38.6857657421267	22.841961511625826	19.23852176972574
27	14.899783525258368	39.731463977529565	24.484008897529325	20.884743599682743
28	18.38693518281083	37.274207302891696	26.3380584064358	18.00079910786167
29	19.132967970087126	38.425757512508426	22.59000399553931	19.851270521865143
30	17.54757375348119	39.003918013918785	24.37368430468671	19.074823927913314
31	19.22778748501673	40.2815960688664	21.882137553895053	18.608478892221818
32	21.819222718517235	37.167758979527335	22.05716602956699	18.955852272388437
33	17.6999409614341	38.4013071973379	23.288627025350802	20.6101248158772
34	18.443588352108392	37.217852308169384	25.21841287637086	19.12014646335136
35	20.700173537602794	35.784527124941114	24.33939422853292	19.17590510892317
36	18.581345005874038	38.01994191559274	26.20775611705141	17.19095696148181
37	17.43635463691282	38.79966842987232	25.71934616279139	18.044630770423467
38	18.887272120080866	37.547335213820986	26.124863585131823	17.440529080966325
39	18.70329840715142	36.83469797897273	23.526272161825307	20.935731452050547
40	19.245976134106996	39.37931980415894	22.052395236362987	19.322308825371078
41	17.0427641975824	37.38751364148682	26.367875863960833	19.201846296969947
42	19.703375932540983	37.477562363212414	24.04628862106186	18.77277308318474
43	17.446492572471332	35.6789733253025	26.68543178660242	20.189102315623753
44	19.93416305378473	34.19406394055592	25.233619779708622	20.638153225950727
45	20.011390268774562	36.13428590170973	25.64301347152731	18.211310357988395
46	17.65521477514655	36.804284172297194	26.49847632792047	19.04202472463578
47	17.816527220356974	35.686725864259	26.38516998932535	20.111576926058667
48	18.983284333311467	35.40733628724946	27.136271744380903	18.473107635058174
49	18.277803288269215	34.79279848765856	25.76794861855719	21.16144960551504
50	17.551748197534693	33.77363778945297	25.559822765032468	23.11479124797987
51	18.20743408851014	33.36484044678478	26.82646836069582	21.601257104009257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19074.0
1	14471.5
2	9869.0
3	5933.0
4	1997.0
5	1649.5
6	1302.0
7	1377.0
8	1452.0
9	1684.0
10	1916.0
11	2357.0
12	2798.0
13	2921.0
14	3044.0
15	3169.5
16	3295.0
17	3083.0
18	2871.0
19	2839.0
20	2807.0
21	2475.0
22	2143.0
23	2314.0
24	2485.0
25	2691.5
26	3650.5
27	4403.0
28	5064.5
29	5726.0
30	6837.0
31	7948.0
32	9175.5
33	10403.0
34	11286.5
35	12170.0
36	12806.5
37	13443.0
38	13120.0
39	12797.0
40	13100.5
41	13404.0
42	15716.5
43	18029.0
44	20843.5
45	23658.0
46	25638.0
47	27618.0
48	28920.0
49	30222.0
50	28341.5
51	26461.0
52	23489.5
53	20518.0
54	17985.0
55	15452.0
56	12690.0
57	9928.0
58	8628.0
59	7328.0
60	6587.5
61	5847.0
62	4779.0
63	3711.0
64	3166.5
65	2622.0
66	2220.0
67	1818.0
68	1455.5
69	1093.0
70	1046.0
71	999.0
72	793.0
73	587.0
74	595.5
75	408.5
76	213.0
77	227.5
78	242.0
79	169.5
80	97.0
81	67.0
82	37.0
83	26.0
84	15.0
85	19.0
86	23.0
87	14.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	335374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.875870750290595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64159726506048	23.04624309751252
2	10.485512839881398	6.47498678389381
3	4.046578421156522	3.7482489693763132
4	2.1490368085384244	2.6541353095219757
5	1.4507348227165076	2.2396350439570316
6	0.9664697897182742	1.790435778684118
7	0.7558013553823907	1.6335217473176802
8	0.5950560978799864	1.4698300133851787
9	0.469255629949297	1.3039808561244621
>10	3.6542033976513997	23.423565216406026
>50	0.5251669363486065	11.29222754019504
>100	0.2525993058863069	11.864067841771893
>500	0.0029952486863988963	0.5077202030712028
>1k	0.003993664915198528	3.321914334119782
>5k	0.0	0.0
>10k+	9.98416228799632E-4	5.2294872646629535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16964	5.058233494546387	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3635	1.0838645810349044	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3410	1.016775301603583	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGCT	2062	0.6148359741661548	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1669	0.49765336609278005	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	573	0.17085403161843196	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCT	554	0.1651887146886759	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	520	0.15505077913016513	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGT	498	0.14849093847465814	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCT	489	0.1458073672974053	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCC	451	0.13447673343789324	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTT	434	0.12940776565863782	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	420	0.12523332160513337	No Hit
CTGTCAGGGATTTTTCCCTAATTTTCTTGCATTTCCTATTTATATAAAGAA	407	0.12135705212687924	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9817457525031755E-4	0.0	0.0	0.7481200093030468	0.0
2	2.9817457525031755E-4	0.0	0.0	1.013197206700579	0.0
3	2.9817457525031755E-4	0.0	0.0	1.2606821041583427	0.0
4	2.9817457525031755E-4	0.0	0.0	2.5926279318015113	0.0
5	2.9817457525031755E-4	0.0	0.0	2.8052264039549875	0.0
6	2.9817457525031755E-4	0.0	0.0	4.022673194702034	0.0
7	2.9817457525031755E-4	0.0	0.0	4.990547865964565	0.0
8	2.9817457525031755E-4	0.0	0.0	5.861813974845993	0.0
9	2.9817457525031755E-4	0.0	0.0	7.219999165111189	0.0
10	2.9817457525031755E-4	0.0	0.0	9.363576186585723	0.0
11	2.9817457525031755E-4	0.0	0.0	11.009798016542726	0.0
12	2.9817457525031755E-4	0.0	0.0	11.854228413651624	0.0
13	2.9817457525031755E-4	0.0	0.0	12.17894052609922	0.0
14	2.9817457525031755E-4	0.0	0.0	12.529892001168845	0.0
15	2.9817457525031755E-4	0.0	0.0	12.81733229171015	0.0
16	2.9817457525031755E-4	0.0	0.0	13.397281840572019	0.0
17	2.9817457525031755E-4	0.0	0.0	14.25363922069093	0.0
18	2.9817457525031755E-4	0.0	0.0	15.340485547478337	0.0
19	2.9817457525031755E-4	0.0	0.0	15.78327479172506	0.0
20	2.9817457525031755E-4	0.0	0.0	16.31193831364387	0.0
21	2.9817457525031755E-4	0.0	0.0	16.805417235683148	0.0
22	2.9817457525031755E-4	0.0	0.0	17.3740361506855	0.0
23	2.9817457525031755E-4	0.0	0.0	17.91731022679158	0.0
24	2.9817457525031755E-4	0.0	0.0	18.30225360343974	0.0
25	2.9817457525031755E-4	0.0	0.0	18.65648499883712	0.0
26	2.9817457525031755E-4	0.0	0.0	18.953765050361685	0.0
27	2.9817457525031755E-4	0.0	0.0	19.31604715929082	0.0
28	2.9817457525031755E-4	0.0	0.0	19.664315063183192	0.0
29	2.9817457525031755E-4	0.0	0.0	20.02898256871433	0.0
30	2.9817457525031755E-4	0.0	0.0	20.511429031469344	0.0
31	2.9817457525031755E-4	0.0	0.0	20.848068126926954	0.0
32	2.9817457525031755E-4	0.0	0.0	21.18321634950831	0.0
33	2.9817457525031755E-4	0.0	0.0	21.532080602551183	0.0
34	2.9817457525031755E-4	0.0	0.0	21.833535098129254	0.0
35	2.9817457525031755E-4	0.0	0.0	22.17226141561361	0.0
36	2.9817457525031755E-4	0.0	0.0	22.494886306034456	0.0
37	2.9817457525031755E-4	0.0	0.0	22.8169148473048	0.0
38	2.9817457525031755E-4	0.0	0.0	23.131190849618633	0.0
39	2.9817457525031755E-4	0.0	0.0	23.488702165343767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.0261327E-4	45.000004	1
TCACGAG	20	7.0261327E-4	45.000004	1
ATGGGTA	70	0.0	45.000004	5
TTAGGTA	20	7.0261327E-4	45.000004	40
AACCGTC	20	7.0261327E-4	45.000004	10
GATACGC	20	7.0261327E-4	45.000004	27
TCGCACG	20	7.0261327E-4	45.000004	1
AGTCATT	20	7.0261327E-4	45.000004	17
GTACACC	20	7.0261327E-4	45.000004	24
CACGGGT	35	1.208482E-7	45.000004	4
CCTAGCG	20	7.0261327E-4	45.000004	1
TTTGTCG	20	7.0261327E-4	45.000004	44
CGTGATC	20	7.0261327E-4	45.000004	12
TTGTCGT	20	7.0261327E-4	45.000004	45
GATCCGT	20	7.0261327E-4	45.000004	35
ACATTGA	20	7.0261327E-4	45.000004	21
CCGGAAG	20	7.0261327E-4	45.000004	1
GGTTTAC	20	7.0261327E-4	45.000004	9
GTCTACG	20	7.0261327E-4	45.000004	34
GGTCAAT	20	7.0261327E-4	45.000004	41
>>END_MODULE