FastQCFastQC Report
Sat 18 Jun 2016
SRR3553345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329830
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166845.058363399326926No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35791.0851044477458085No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33201.006579146833217No Hit
CTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGCT18720.5675651092987296No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16630.5041991328866385No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5760.17463541824576295No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA5050.1531091774550526No Hit
AATCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCT4950.15007731255495255No Hit
CTGTCAGGGATTTTTCCCTAATTTTCTTGCATTTCCTATTTATATAAAGAA4590.13916259891459237No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGT4520.13704029348452232No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTT4430.13431161507443226No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCC4110.12460964739411211No Hit
AAACTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCT4110.12460964739411211No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3980.12066822302398204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGTT302.1607775E-645.00000437
AAGTACG302.1607775E-645.0000041
CCTTACT302.1607775E-645.00000412
GGCAATC302.1607775E-645.0000048
CACGACT351.2084092E-745.0000044
CCTCGTA351.2084092E-745.00000416
ACCCTCG351.2084092E-745.00000414
TACATGG700.045.0000042
TCTACGG351.2084092E-745.0000042
CTACGAA600.045.00000410
CGGGCTA302.1607775E-645.0000046
CGTACTG302.1607775E-645.0000041
TCGTAAT351.2084092E-745.00000418
CGTAATG351.2084092E-745.0000041
CGTAATA351.2084092E-745.00000419
ATAACGG302.1607775E-645.0000042
GCAATCA302.1607775E-645.0000049
ACGTAGG351.2084092E-745.0000042
GCTACGA600.045.0000049
CGTCCTA351.2084092E-745.00000436