##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553345_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329830
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40724009338144	31.0	31.0	34.0	30.0	34.0
2	31.600084892217204	31.0	31.0	34.0	30.0	34.0
3	31.818354910105207	31.0	31.0	34.0	30.0	34.0
4	35.576554588727525	37.0	35.0	37.0	33.0	37.0
5	35.32914531728466	37.0	35.0	37.0	33.0	37.0
6	32.58550162204772	37.0	35.0	37.0	26.0	37.0
7	34.143131310068824	37.0	35.0	37.0	27.0	37.0
8	35.209517023921414	37.0	35.0	37.0	32.0	37.0
9	36.997228875481305	39.0	37.0	39.0	33.0	39.0
10	36.47454446229876	38.0	35.0	39.0	32.0	39.0
11	36.61537458690841	39.0	35.0	39.0	32.0	39.0
12	36.66879907831307	39.0	35.0	39.0	32.0	39.0
13	36.65338507716096	39.0	35.0	39.0	32.0	39.0
14	37.734393475426735	39.0	37.0	41.0	33.0	41.0
15	37.80535124154868	39.0	37.0	41.0	33.0	41.0
16	37.71977079101355	39.0	37.0	41.0	33.0	41.0
17	37.67005427038171	39.0	37.0	40.0	33.0	41.0
18	37.43908680229209	39.0	36.0	40.0	32.0	41.0
19	37.25615620167965	39.0	35.0	40.0	32.0	41.0
20	37.11774247339539	39.0	35.0	40.0	32.0	41.0
21	37.04509595852409	39.0	35.0	40.0	32.0	41.0
22	36.97218263954158	39.0	35.0	40.0	31.0	41.0
23	36.7576175605615	39.0	35.0	40.0	31.0	41.0
24	36.654036928114486	38.0	35.0	40.0	31.0	41.0
25	36.69941788193918	38.0	35.0	40.0	31.0	41.0
26	36.61672073492405	38.0	35.0	40.0	31.0	41.0
27	36.50238001394658	38.0	35.0	40.0	31.0	41.0
28	36.46341448625049	38.0	35.0	40.0	31.0	41.0
29	36.23981444986811	38.0	35.0	40.0	30.0	41.0
30	36.20345632598612	38.0	35.0	40.0	30.0	41.0
31	35.77784009944517	38.0	35.0	40.0	29.0	41.0
32	35.858081435891215	38.0	35.0	40.0	29.0	41.0
33	35.81886729527332	38.0	35.0	40.0	30.0	41.0
34	35.79245975199345	38.0	35.0	40.0	29.0	41.0
35	35.73218930964436	38.0	35.0	40.0	29.0	41.0
36	35.58204529606161	38.0	35.0	40.0	29.0	41.0
37	35.478755722645	38.0	34.0	40.0	28.0	41.0
38	35.42816299305703	38.0	34.0	40.0	27.0	41.0
39	35.35383985689597	38.0	34.0	40.0	27.0	41.0
40	35.19345420368069	38.0	34.0	40.0	26.0	41.0
41	35.16946608859109	38.0	34.0	40.0	26.0	41.0
42	35.044428948246065	38.0	34.0	40.0	26.0	41.0
43	35.01696631598096	38.0	34.0	40.0	26.0	41.0
44	34.82916350847406	37.0	34.0	40.0	25.0	41.0
45	34.658345208137526	37.0	33.0	40.0	24.0	41.0
46	34.36510020313495	37.0	33.0	40.0	23.0	41.0
47	34.362802049540676	37.0	33.0	40.0	23.0	41.0
48	34.31499863566079	37.0	33.0	40.0	23.0	41.0
49	34.18435557711548	37.0	33.0	40.0	23.0	41.0
50	34.05207227965922	36.0	33.0	40.0	23.0	41.0
51	33.00762817208865	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	3.0
12	1.0
13	2.0
14	7.0
15	15.0
16	37.0
17	113.0
18	247.0
19	471.0
20	748.0
21	1268.0
22	1619.0
23	2112.0
24	2606.0
25	3121.0
26	3364.0
27	4101.0
28	4981.0
29	6244.0
30	7751.0
31	10304.0
32	13288.0
33	17511.0
34	24291.0
35	38402.0
36	35980.0
37	44489.0
38	56141.0
39	50599.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.4454719097717	36.696176818360975	19.604038444046935	24.254312827820392
2	18.476184701209714	38.91610829821423	20.303489676500014	22.30421732407604
3	18.992814480186762	38.94945881211533	19.814753054603887	22.242973653094015
4	17.554497771579296	39.044659369978476	19.751993451171813	23.64884940727041
5	16.256253221356456	41.46742261164842	17.15792984264621	25.11839432434891
6	16.071612648940363	47.76733468756632	17.256162265409454	18.90489039808386
7	76.87384410150683	15.027741563835914	4.565685353060667	3.53272898159658
8	78.01473486341447	14.446229875996725	3.5215110814662096	4.017524179122578
9	71.1390716429676	18.025043204074827	7.0681866416032495	3.7676985113543338
10	24.923748597762486	52.585877573295335	10.394748809993025	12.095625018949157
11	17.097292544644212	35.574083618833946	32.386380862868755	14.942242973653094
12	20.58424036624928	31.948579571294307	30.74765788436467	16.719522178091744
13	20.57029378770882	34.14425613194676	26.878391898856986	18.407058181487432
14	15.792681078131157	36.874450474486856	27.70851650850438	19.624351938877606
15	14.575387320740987	39.47033320195252	27.850710972319074	18.103568504987418
16	16.914471091168178	39.911469544917075	24.57781281266107	18.596246551253675
17	16.861716641906437	38.17451414364976	25.572870872873903	19.3908983415699
18	17.359852044992873	38.47800382014977	24.740927144286452	19.4212169905709
19	16.311129976048267	40.76706181972531	25.540429918442836	17.381378285783587
20	17.72185671406482	39.151684200952005	25.519206864142134	17.607252220841037
21	17.108813631264592	40.03547281933117	25.54891914016311	17.306794409241125
22	17.262225995209654	37.373192250553316	25.501622047721558	19.86295970651548
23	16.852014674226115	39.37119121971925	23.47482036200467	20.301973744049963
24	13.623988115089592	39.88357638783616	26.49243549707425	20.0
25	16.286268683867448	40.64305854531122	24.591759391201528	18.478913379619804
26	19.094685140830126	38.776036139829614	22.89148955522542	19.237789164114847
27	14.927386835642602	39.84294939817482	24.432889670436285	20.796774095746294
28	18.3455113240154	37.24949216262923	26.4757602401237	17.929236273231663
29	19.145620471151805	38.65112330594549	22.601643270775853	19.601612952126853
30	17.433223175575296	39.22141709365431	24.301306733771945	19.044052996998452
31	19.43789224752145	40.26013400842858	21.605069278112968	18.696904465937
32	21.74514143649759	37.17672740502683	22.061364945578024	19.016766212897554
33	17.64878876997241	38.58715095655337	23.230755237546617	20.533305035927597
34	18.35187824030561	37.220386259588274	25.363369008276994	19.064366491829123
35	20.55968226055847	35.86787132765364	24.31070551496225	19.26174089682564
36	18.463754055119306	38.2515235121123	26.109814146681625	17.174908286086772
37	17.516296273838037	38.721159385137796	25.699906012188094	18.062638328836066
38	18.89245975199345	37.676985113543346	25.90425370645484	17.526301428008367
39	18.527423218021404	37.12912712609526	23.35657763090077	20.986872024982567
40	19.36391474395901	39.65800563926871	22.037110026377224	18.940969590395053
41	16.971166964800048	37.37864960737349	26.360246187429887	19.28993724039657
42	19.624958311857625	37.74035108995543	24.03541218203317	18.599278416153776
43	17.59542794773065	35.76569748052027	26.40936239881151	20.229512172937575
44	19.94936785616833	34.43743746778644	25.200254676651607	20.41293999939363
45	19.832944244004487	36.32022557074856	25.649880241336447	18.196949943910496
46	17.73459054664524	36.97510838917018	26.3878361580208	18.902464906163782
47	17.73671285207531	35.686565806627655	26.48970681866416	20.08701452263287
48	18.83242882697147	35.64260376557621	26.89324803686748	18.631719370584847
49	18.23969923900191	35.021677834035714	25.577721856714064	21.160901070248308
50	17.399569475184183	33.985386411181516	25.54497771579298	23.070066397841313
51	18.023830458114787	33.77679410605464	26.630688536518814	21.568686899311768
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18825.0
1	14300.5
2	9776.0
3	5877.0
4	1978.0
5	1621.0
6	1264.0
7	1411.5
8	1559.0
9	1706.0
10	1853.0
11	2340.0
12	2827.0
13	2966.0
14	3105.0
15	3216.5
16	3328.0
17	3065.5
18	2803.0
19	2772.5
20	2742.0
21	2467.0
22	2192.0
23	2296.0
24	2400.0
25	2645.5
26	3596.0
27	4301.0
28	5034.0
29	5767.0
30	6803.0
31	7839.0
32	9148.5
33	10458.0
34	11244.0
35	12030.0
36	12626.0
37	13222.0
38	12930.5
39	12639.0
40	12909.5
41	13180.0
42	15383.5
43	17587.0
44	20333.0
45	23079.0
46	25032.0
47	26985.0
48	28249.5
49	29514.0
50	27516.0
51	25518.0
52	22800.0
53	20082.0
54	17572.0
55	15062.0
56	12462.5
57	9863.0
58	8522.5
59	7182.0
60	6519.0
61	5856.0
62	4767.0
63	3678.0
64	3166.0
65	2654.0
66	2231.5
67	1809.0
68	1503.5
69	1198.0
70	1088.0
71	978.0
72	775.0
73	572.0
74	595.5
75	412.5
76	206.0
77	229.0
78	252.0
79	160.5
80	69.0
81	56.5
82	44.0
83	29.0
84	14.0
85	17.0
86	20.0
87	13.5
88	7.0
89	4.0
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	329830.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.428308811833567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9989822501077	23.57091174729609
2	10.115894601487458	6.358509188870157
3	3.90025161222262	3.6773493633835272
4	2.2247731609643395	2.796834317562655
5	1.4385911093478965	2.260624281927196
6	0.9923102720664483	1.8711980200595348
7	0.8136116471096944	1.7899306668787622
8	0.6229367336899236	1.5662278429313474
9	0.4901633312940742	1.3864504089732523
>10	3.6517667031417975	23.477101233127513
>50	0.5101292468726961	11.187923675866617
>100	0.23360125982037355	11.209572256411073
>500	0.001996591964276697	0.33916109519649495
>1k	0.003993183928553394	3.273641875374864
>5k	0.0	0.0
>10k+	9.982959821383485E-4	5.234564026140908
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16684	5.058363399326926	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3579	1.0851044477458085	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3320	1.006579146833217	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGCT	1872	0.5675651092987296	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1663	0.5041991328866385	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	576	0.17463541824576295	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	505	0.1531091774550526	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCT	495	0.15007731255495255	No Hit
CTGTCAGGGATTTTTCCCTAATTTTCTTGCATTTCCTATTTATATAAAGAA	459	0.13916259891459237	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGT	452	0.13704029348452232	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTT	443	0.13431161507443226	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCC	411	0.12460964739411211	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCT	411	0.12460964739411211	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	398	0.12066822302398204	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6782281781523816	0.0
2	0.0	0.0	0.0	0.9489737137313161	0.0
3	0.0	0.0	0.0	1.2057726707697904	0.0
4	0.0	0.0	0.0	2.462480671861262	0.0
5	0.0	0.0	0.0	2.666525179637995	0.0
6	0.0	0.0	0.0	3.8077191280356546	0.0
7	0.0	0.0	0.0	4.743352636206531	0.0
8	0.0	0.0	0.0	5.592274808234545	0.0
9	0.0	0.0	0.0	6.976017948640209	0.0
10	0.0	0.0	0.0	9.103174362550405	0.0
11	0.0	0.0	0.0	10.701876724373163	0.0
12	0.0	0.0	0.0	11.511991025679896	0.0
13	0.0	0.0	0.0	11.81972531304005	0.0
14	0.0	0.0	0.0	12.156868689931176	0.0
15	0.0	0.0	0.0	12.448837279810812	0.0
16	0.0	0.0	0.0	13.01337052420944	0.0
17	0.0	0.0	0.0	13.834096352666526	0.0
18	0.0	0.0	0.0	14.949822635903343	0.0
19	0.0	0.0	0.0	15.384895249067702	0.0
20	0.0	0.0	0.0	15.9000090955947	0.0
21	0.0	0.0	0.0	16.388745717490828	0.0
22	0.0	0.0	0.0	16.933268653548797	0.0
23	0.0	0.0	0.0	17.429888124185187	0.0
24	0.0	0.0	0.0	17.78400994451687	0.0
25	0.0	0.0	0.0	18.105387623927477	0.0
26	0.0	0.0	0.0	18.394021162417	0.0
27	0.0	0.0	0.0	18.74814298274869	0.0
28	0.0	0.0	0.0	19.063456932359095	0.0
29	0.0	0.0	0.0	19.4272807203711	0.0
30	0.0	0.0	0.0	19.881454082406087	0.0
31	3.0318649001000517E-4	0.0	0.0	20.211017797046964	0.0
32	3.0318649001000517E-4	0.0	0.0	20.526634933147378	0.0
33	3.0318649001000517E-4	0.0	0.0	20.854379528848195	0.0
34	3.0318649001000517E-4	0.0	0.0	21.17515083527878	0.0
35	3.0318649001000517E-4	0.0	0.0	21.517448382500074	0.0
36	3.0318649001000517E-4	0.0	0.0	21.84307067277082	0.0
37	3.0318649001000517E-4	0.0	0.0	22.187793711912196	0.0
38	3.0318649001000517E-4	0.0	0.0	22.493102507352273	0.0
39	3.0318649001000517E-4	0.0	0.0	22.854803989934208	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGTT	30	2.1607775E-6	45.000004	37
AAGTACG	30	2.1607775E-6	45.000004	1
CCTTACT	30	2.1607775E-6	45.000004	12
GGCAATC	30	2.1607775E-6	45.000004	8
CACGACT	35	1.2084092E-7	45.000004	4
CCTCGTA	35	1.2084092E-7	45.000004	16
ACCCTCG	35	1.2084092E-7	45.000004	14
TACATGG	70	0.0	45.000004	2
TCTACGG	35	1.2084092E-7	45.000004	2
CTACGAA	60	0.0	45.000004	10
CGGGCTA	30	2.1607775E-6	45.000004	6
CGTACTG	30	2.1607775E-6	45.000004	1
TCGTAAT	35	1.2084092E-7	45.000004	18
CGTAATG	35	1.2084092E-7	45.000004	1
CGTAATA	35	1.2084092E-7	45.000004	19
ATAACGG	30	2.1607775E-6	45.000004	2
GCAATCA	30	2.1607775E-6	45.000004	9
ACGTAGG	35	1.2084092E-7	45.000004	2
GCTACGA	60	0.0	45.000004	9
CGTCCTA	35	1.2084092E-7	45.000004	36
>>END_MODULE