SRR3553344_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553344_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	593882
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29940	5.041405531738628	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7041	1.1855890564118798	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5545	0.9336871634432429	No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	5044	0.8493269706776767	Illumina Paired End PCR Primer 2 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGT	4639	0.7811316052683867	No Hit
AATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	3419	0.5757035909490438	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3413	0.5746932892392765	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC	2980	0.5017831825177392	No Hit
AAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	1722	0.2899565907032037	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	1597	0.2689086384163858	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	1231	0.20728023412058286	No Hit
CGGCCAGGGGAAATTACATCTGATTTAATTATCTGTTTTTATTTTTATTTA	973	0.16383726060059067	No Hit
GACCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATC	855	0.14396799364183457	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	842	0.1417790066040055	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	811	0.13655911443687468	No Hit
AACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	767	0.12915023523191474	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	746	0.12561417924772936	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	638	0.10742874847191866	TruSeq Adapter, Index 14 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCAC	20	7.030529E-4	45.0	42
AGACTCG	25	3.8885213E-5	45.0	1
AATCCGA	20	7.030529E-4	45.0	28
GGAGCGC	40	6.8048394E-9	45.0	8
CGAACAG	20	7.030529E-4	45.0	1
GTCGACG	20	7.030529E-4	45.0	14
ACGTTGT	20	7.030529E-4	45.0	37
CGACGCG	20	7.030529E-4	45.0	16
CGTGCGG	20	7.030529E-4	45.0	2
CCGATAT	20	7.030529E-4	45.0	31
TTTCGCG	35	1.2106102E-7	45.0	1
GACGTCG	20	7.030529E-4	45.0	11
GACAACG	45	3.8380676E-10	45.0	1
TAGCGAG	40	6.8048394E-9	45.0	1
GGTCCCG	20	7.030529E-4	45.0	9
TCGGGCA	35	1.2106102E-7	45.0	5
CCTACGC	20	7.030529E-4	45.0	23
ATTAACG	20	7.030529E-4	45.0	1
GTCTAGA	25	3.8885213E-5	45.0	29
TGCGGTG	20	7.030529E-4	45.0	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content