##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553344_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	593882
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.370147605079797	31.0	31.0	34.0	28.0	34.0
2	31.564447146066055	31.0	31.0	34.0	30.0	34.0
3	31.768523039930493	31.0	31.0	34.0	30.0	34.0
4	35.517346880356705	37.0	35.0	37.0	33.0	37.0
5	35.236506578747964	37.0	35.0	37.0	33.0	37.0
6	33.91007978015835	37.0	35.0	37.0	30.0	37.0
7	34.68879676434039	37.0	35.0	37.0	32.0	37.0
8	35.36310580216272	37.0	35.0	37.0	32.0	37.0
9	36.972373299746415	39.0	37.0	39.0	33.0	39.0
10	36.429848016946124	38.0	35.0	39.0	32.0	39.0
11	36.51553844029622	38.0	35.0	39.0	32.0	39.0
12	36.563623750172596	38.0	35.0	39.0	32.0	39.0
13	36.53853964255526	38.0	35.0	39.0	32.0	39.0
14	37.666817650644404	39.0	37.0	41.0	32.0	41.0
15	37.67282221047279	39.0	37.0	41.0	32.0	41.0
16	37.53361778939251	39.0	36.0	41.0	32.0	41.0
17	37.448408943190735	39.0	36.0	40.0	32.0	41.0
18	37.219479627266026	39.0	36.0	40.0	32.0	41.0
19	37.0168046851058	39.0	35.0	40.0	32.0	41.0
20	36.94028780128039	39.0	35.0	40.0	31.0	41.0
21	36.876728036882746	39.0	35.0	40.0	31.0	41.0
22	36.85446098719948	39.0	35.0	40.0	31.0	41.0
23	36.631672621833964	38.0	35.0	40.0	31.0	41.0
24	36.48556615623979	38.0	35.0	40.0	31.0	41.0
25	36.56921408629997	38.0	35.0	40.0	31.0	41.0
26	36.46501493562694	38.0	35.0	40.0	31.0	41.0
27	36.42406740733008	38.0	35.0	40.0	31.0	41.0
28	36.329073789069206	38.0	35.0	40.0	30.0	41.0
29	36.205027261307805	38.0	35.0	40.0	30.0	41.0
30	36.22802509589447	38.0	35.0	40.0	30.0	41.0
31	36.06688028935041	38.0	35.0	40.0	30.0	41.0
32	35.98750425168636	38.0	35.0	40.0	30.0	41.0
33	35.88647239687345	38.0	35.0	40.0	30.0	41.0
34	35.81110220548863	38.0	35.0	40.0	29.0	41.0
35	35.69670742672787	38.0	35.0	40.0	29.0	41.0
36	35.594517092621096	38.0	35.0	40.0	28.0	41.0
37	35.51648980773958	38.0	35.0	40.0	28.0	41.0
38	35.37732411489151	38.0	34.0	40.0	27.0	41.0
39	35.229055603638436	38.0	34.0	40.0	26.0	41.0
40	35.02261728760932	38.0	34.0	40.0	25.0	41.0
41	34.93949976594677	38.0	33.0	40.0	25.0	41.0
42	34.83182349355596	38.0	33.0	40.0	24.0	41.0
43	34.77988725032919	37.0	33.0	40.0	24.0	41.0
44	34.688266355942766	37.0	33.0	40.0	24.0	41.0
45	34.414131090014514	37.0	33.0	40.0	23.0	41.0
46	34.20923853560135	37.0	33.0	40.0	23.0	41.0
47	34.11647768411907	37.0	33.0	40.0	23.0	41.0
48	33.90557383453279	37.0	33.0	40.0	22.0	41.0
49	33.7221771328311	36.0	33.0	40.0	22.0	41.0
50	33.60325957008295	36.0	33.0	40.0	21.0	41.0
51	32.39452618533648	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	6.0
12	9.0
13	8.0
14	15.0
15	41.0
16	95.0
17	183.0
18	416.0
19	759.0
20	1456.0
21	2375.0
22	3143.0
23	4055.0
24	4913.0
25	5736.0
26	6560.0
27	7698.0
28	9795.0
29	12088.0
30	15267.0
31	19225.0
32	24545.0
33	31613.0
34	43568.0
35	69174.0
36	63302.0
37	76410.0
38	98885.0
39	92524.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.074802738591167	38.257599994611724	20.25823311701651	23.409364149780597
2	17.683310826056356	40.235770742336015	19.68337144415894	22.397546987448685
3	16.210796084070573	38.79373343526155	21.412839587662198	23.582630893005682
4	17.844959099619118	37.35169612818708	18.34724743299174	26.45609733920206
5	14.20602072465574	42.11947828019708	17.336945723224478	26.337555271922707
6	15.884805399052338	43.505107075142874	20.857847181763383	19.752240344041407
7	71.70262779474712	16.503615196284784	6.874092833256438	4.919664175711674
8	73.79681485547634	14.157526242586913	4.8837984650149355	7.161860436921813
9	67.5524767546415	18.449119522059938	9.34579596620204	4.652607757096527
10	23.541208522905226	50.633795939260665	12.545421481034952	13.279574056799163
11	15.41501510401056	35.616166174425224	33.75485365779734	15.213965063766876
12	21.190909978749985	30.988142425599698	32.124731849087865	15.696215746562448
13	19.94015646205812	33.918185767543044	25.112227681593314	21.02943008880552
14	14.324562791935097	35.24269130904793	29.383783310489285	21.048962588527687
15	12.476552581152484	40.72004202855113	26.570598199642355	20.232807190654036
16	14.698542808167279	38.65532210102344	25.45337289225806	21.192762198551225
17	13.118599317709577	39.200211489824575	27.311149352901754	20.37003983956409
18	14.42138337245446	38.280668550318076	24.58299796929356	22.7149501079339
19	15.047602048891868	40.77981821304569	26.455592188347183	17.716987549715263
20	16.248682398186844	39.38981144402424	25.902957153104488	18.458549004684432
21	15.449533745760943	40.41375222687335	23.56596091479452	20.570753112571182
22	16.319066750633965	38.322932838510006	25.205007055273605	20.15299335558242
23	16.29818718196544	38.30373710602443	22.721348685429092	22.676727026581037
24	12.494401244691707	40.81383170394119	25.63943679047353	21.05233026089358
25	14.527465051980023	39.89361522996151	23.743942399331853	21.834977318726615
26	16.7221771328311	39.77423124459067	21.896605723022418	21.606985899555802
27	13.061685654726023	39.1963386666038	23.69090155956907	24.051074119101102
28	16.002673931858517	36.02752735391879	26.049450901020744	21.92034781320195
29	15.919997575275897	36.36395782327129	23.710602442909533	24.005442158543282
30	15.514698206040931	37.6411475680354	21.738661889062136	25.10549233686153
31	16.088886344425323	37.83832478505831	23.689722874241014	22.383065996275352
32	15.850286757301957	35.622901519157004	22.799647067936053	25.727164655604984
33	17.216383052525586	36.71200676228611	22.421289077628217	23.65032110756009
34	13.022789038899985	34.46391707443566	27.669638076250834	24.843655810413516
35	17.24551341849054	34.21757184087075	24.361068360381356	24.175846380257358
36	15.166649267026107	34.82644700462381	28.62504672645408	21.381857001895998
37	15.180793490962852	36.86624615664391	27.066993106374664	20.88596724601857
38	17.809430156158967	34.91754254212116	29.942816923227173	17.330210378492698
39	16.52617860113625	35.35365611350403	29.04516385409896	19.075001431260755
40	19.507915713896026	38.36435520861046	24.130382803317833	17.997346274175676
41	15.036320346466134	33.922395358000415	30.48686439393684	20.554419901596614
42	16.993948292758493	35.11354107381601	24.568180210883643	23.324330422541852
43	15.293610515220196	35.74716862945838	25.76505096972126	23.194169885600168
44	17.232379496263565	32.18029844312507	24.791793655978797	25.795528404632574
45	18.852229904257076	35.222316891234286	25.943705988731768	19.98174721577687
46	14.741143863595799	35.42454561680603	29.358189000508517	20.476121519089652
47	17.95592390407522	34.17429725096905	25.124519685728814	22.745259159226915
48	15.773335443741349	32.55040563613647	32.011409673975635	19.66484924614654
49	17.01886906826609	33.05926093062258	27.17021226438922	22.75165773672211
50	16.579219440899035	30.512963854772497	28.109456087236182	24.798360617092285
51	15.003317157280401	30.79904762225493	30.617361698115115	23.580273522349557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34244.0
1	25680.0
2	17116.0
3	10307.0
4	3498.0
5	2865.0
6	2232.0
7	2325.0
8	2418.0
9	2950.0
10	3482.0
11	3823.5
12	4165.0
13	4705.0
14	5245.0
15	5245.0
16	5245.0
17	4870.5
18	4496.0
19	4313.0
20	4130.0
21	4157.0
22	4184.0
23	4431.5
24	4679.0
25	5547.5
26	7343.5
27	8271.0
28	9153.5
29	10036.0
30	11436.0
31	12836.0
32	14551.0
33	16266.0
34	17811.0
35	19356.0
36	21337.5
37	23319.0
38	22621.5
39	21924.0
40	22616.5
41	23309.0
42	27009.5
43	30710.0
44	40822.5
45	50935.0
46	52367.0
47	53799.0
48	56069.5
49	58340.0
50	52236.0
51	46132.0
52	39830.5
53	33529.0
54	28069.5
55	22610.0
56	19988.0
57	17366.0
58	14985.0
59	12604.0
60	11347.0
61	10090.0
62	8376.5
63	6663.0
64	6134.0
65	5605.0
66	4310.0
67	3015.0
68	2650.0
69	2285.0
70	1750.5
71	1216.0
72	1037.5
73	859.0
74	654.0
75	430.0
76	411.0
77	315.5
78	220.0
79	149.5
80	79.0
81	64.5
82	50.0
83	39.5
84	29.0
85	18.0
86	7.0
87	8.0
88	9.0
89	5.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	593882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.31664071074243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.58193177482374	21.685430470022734
2	9.712441803252393	5.500474499333867
3	3.7985170150737666	3.2268372464845685
4	1.9559949446522273	2.215488243189826
5	1.2736720005895708	1.8033056212013696
6	0.8892514579761535	1.5108368422208767
7	0.6313493023342349	1.2514383950023502
8	0.5229780983308829	1.184718632801835
9	0.3950486895264478	1.0067806624113051
>10	3.3248230686613685	19.3441245772092
>50	0.5422898568238282	10.891111851304009
>100	0.3573366937349067	17.5880151391238
>500	0.007781201036103495	1.5082947549683212
>1k	0.004189877480978805	3.220486418603559
>5k	0.0017956617775623453	2.9881151286764247
>10k+	5.98553925854115E-4	5.074541517445954
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29940	5.041405531738628	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7041	1.1855890564118798	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5545	0.9336871634432429	No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	5044	0.8493269706776767	Illumina Paired End PCR Primer 2 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGT	4639	0.7811316052683867	No Hit
AATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	3419	0.5757035909490438	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3413	0.5746932892392765	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC	2980	0.5017831825177392	No Hit
AAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	1722	0.2899565907032037	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	1597	0.2689086384163858	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	1231	0.20728023412058286	No Hit
CGGCCAGGGGAAATTACATCTGATTTAATTATCTGTTTTTATTTTTATTTA	973	0.16383726060059067	No Hit
GACCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATC	855	0.14396799364183457	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	842	0.1417790066040055	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	811	0.13655911443687468	No Hit
AACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	767	0.12915023523191474	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	746	0.12561417924772936	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	638	0.10742874847191866	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6838361829454336E-4	0.0	0.0	1.0938199844413536	0.0
2	1.6838361829454336E-4	0.0	0.0	1.4243570271535422	0.0
3	1.6838361829454336E-4	0.0	0.0	1.926308593289576	0.0
4	1.6838361829454336E-4	0.0	0.0	4.438592178244163	0.0
5	1.6838361829454336E-4	0.0	0.0	4.773843962268598	0.0
6	1.6838361829454336E-4	0.0	0.0	6.640881521918495	0.0
7	1.6838361829454336E-4	0.0	0.0	8.112722729431098	0.0
8	1.6838361829454336E-4	0.0	0.0	9.08934771553945	0.0
9	1.6838361829454336E-4	0.0	0.0	11.30409744696758	0.0
10	1.6838361829454336E-4	0.0	0.0	13.334467116363184	0.0
11	1.6838361829454336E-4	0.0	0.0	15.457784543057375	0.0
12	1.6838361829454336E-4	0.0	0.0	16.439461037714562	0.0
13	1.6838361829454336E-4	0.0	0.0	16.868670880747352	0.0
14	1.6838361829454336E-4	0.0	0.0	17.332736132767117	0.0
15	1.6838361829454336E-4	0.0	0.0	17.675901946851393	0.0
16	1.6838361829454336E-4	0.0	0.0	18.33024068754399	0.0
17	1.6838361829454336E-4	0.0	0.0	19.187986839136393	0.0
18	1.6838361829454336E-4	0.0	0.0	20.522763781357238	0.0
19	1.6838361829454336E-4	0.0	0.0	20.940186771109413	0.0
20	1.6838361829454336E-4	0.0	0.0	21.4497156000687	0.0
21	1.6838361829454336E-4	0.0	0.0	21.925062554514195	0.0
22	1.6838361829454336E-4	0.0	0.0	22.471299012261696	0.0
23	1.6838361829454336E-4	0.0	0.0	23.006758918438344	0.0
24	1.6838361829454336E-4	0.0	0.0	23.38023378381564	0.0
25	1.6838361829454336E-4	0.0	0.0	23.688712572531244	0.0
26	1.6838361829454336E-4	0.0	0.0	23.96098888331352	0.0
27	1.6838361829454336E-4	0.0	0.0	24.29152592602571	0.0
28	1.6838361829454336E-4	0.0	0.0	24.611454800785342	0.0
29	1.6838361829454336E-4	0.0	0.0	24.950411024412258	0.0
30	1.6838361829454336E-4	0.0	0.0	25.41161375492101	0.0
31	1.6838361829454336E-4	0.0	0.0	25.730195560734288	0.0
32	1.6838361829454336E-4	0.0	0.0	26.038000814976712	0.0
33	1.6838361829454336E-4	0.0	0.0	26.331998612518984	0.0
34	1.6838361829454336E-4	0.0	0.0	26.630374384136918	0.0
35	1.6838361829454336E-4	0.0	0.0	26.978423323151738	0.0
36	1.6838361829454336E-4	0.0	0.0	27.285891810157572	0.0
37	1.6838361829454336E-4	0.0	0.0	27.605483917680615	0.0
38	1.6838361829454336E-4	0.0	0.0	27.904869991008315	0.0
39	1.6838361829454336E-4	0.0	0.0	28.195500116184697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCAC	20	7.030529E-4	45.0	42
AGACTCG	25	3.8885213E-5	45.0	1
AATCCGA	20	7.030529E-4	45.0	28
GGAGCGC	40	6.8048394E-9	45.0	8
CGAACAG	20	7.030529E-4	45.0	1
GTCGACG	20	7.030529E-4	45.0	14
ACGTTGT	20	7.030529E-4	45.0	37
CGACGCG	20	7.030529E-4	45.0	16
CGTGCGG	20	7.030529E-4	45.0	2
CCGATAT	20	7.030529E-4	45.0	31
TTTCGCG	35	1.2106102E-7	45.0	1
GACGTCG	20	7.030529E-4	45.0	11
GACAACG	45	3.8380676E-10	45.0	1
TAGCGAG	40	6.8048394E-9	45.0	1
GGTCCCG	20	7.030529E-4	45.0	9
TCGGGCA	35	1.2106102E-7	45.0	5
CCTACGC	20	7.030529E-4	45.0	23
ATTAACG	20	7.030529E-4	45.0	1
GTCTAGA	25	3.8885213E-5	45.0	29
TGCGGTG	20	7.030529E-4	45.0	42
>>END_MODULE