SRR3553342_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553342_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1354898
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15814	1.1671727318218788	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	3950	0.2915348609268004	RNA PCR Primer, Index 40 (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3715	0.2741903818590034	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3134	0.2313089250998968	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGT	2813	0.2076171047562252	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC	2365	0.17455188508655264	No Hit
AATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	2299	0.1696806696887884	TruSeq Adapter, Index 19 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1777	0.13115378427010743	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1592	0.11749961989758638	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACT	30	2.1657906E-6	45.000004	23
TAGGTCG	30	2.1657906E-6	45.000004	15
CGAAGTA	35	1.2121382E-7	45.000004	19
CGAACTA	25	3.891177E-5	45.0	12
CCGATAG	45	3.8562575E-10	45.0	1
CGTGCAA	20	7.033734E-4	45.0	13
CGTTGTC	20	7.033734E-4	45.0	21
ATTGTCG	25	3.891177E-5	45.0	14
TAACGGC	25	3.891177E-5	45.0	34
TAGCCGT	55	1.8189894E-12	45.0	44
CCGTCTA	20	7.033734E-4	45.0	31
CCGTAAC	20	7.033734E-4	45.0	29
CGTACTA	20	7.033734E-4	45.0	14
CGTAATC	20	7.033734E-4	45.0	44
TTACGCC	20	7.033734E-4	45.0	15
ATAGTCG	25	3.891177E-5	45.0	20
CGCGTGA	20	7.033734E-4	45.0	40
CGATCAC	20	7.033734E-4	45.0	29
GATTACC	20	7.033734E-4	45.0	34
CGATATA	25	3.891177E-5	45.0	29