FastQCFastQC Report
Sat 18 Jun 2016
SRR3553340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916210
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA152401.6633741172875214No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32650.35635934993069274No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27860.3040787592364196No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT22440.24492201569509173No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA21640.2361903930321651No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15210.1660099758788924No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12110.13217493806005173No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG10550.11514827386734483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG302.1650176E-645.00000426
CGTATAG351.2115743E-745.0000041
CGAAATA302.1650176E-645.0000045
CTATCGC351.2115743E-745.00000417
GCGAAAT302.1650176E-645.0000044
TCCGAAC302.1650176E-645.00000423
ACTCTCG302.1650176E-645.00000437
TTCACGC351.2115743E-745.00000420
TAAACGG1200.045.0000042
GCACGAG700.045.0000041
ATATGCG600.045.0000041
TAACGCG700.045.0000041
CCGTCTA302.1650176E-645.00000436
CATACGC302.1650176E-645.00000417
GTTCACG351.2115743E-745.00000419
ATAACGG1300.045.0000042
ACCGTCA302.1650176E-645.00000439
GCGTGCA351.2115743E-745.00000419
GGCGATA700.045.0000048
CTCTAGG600.045.0000042