##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553340_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	916210
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68663625151439	31.0	31.0	34.0	30.0	34.0
2	31.895335130592333	33.0	31.0	34.0	30.0	34.0
3	32.056435751628996	33.0	31.0	34.0	30.0	34.0
4	35.77944576025147	37.0	35.0	37.0	35.0	37.0
5	35.52001724495476	37.0	35.0	37.0	33.0	37.0
6	34.18210563080517	37.0	35.0	37.0	32.0	37.0
7	34.952259853090446	37.0	35.0	37.0	32.0	37.0
8	35.6250630314011	37.0	35.0	37.0	33.0	37.0
9	37.304792569389114	39.0	37.0	39.0	34.0	39.0
10	36.78800602481964	39.0	37.0	39.0	32.0	39.0
11	36.794347365778584	39.0	37.0	39.0	32.0	39.0
12	36.71663483262571	39.0	35.0	39.0	32.0	39.0
13	36.69309874373779	39.0	35.0	39.0	32.0	39.0
14	37.89560471944205	40.0	37.0	41.0	33.0	41.0
15	37.885824210606735	40.0	37.0	41.0	33.0	41.0
16	37.80840746117156	40.0	37.0	41.0	33.0	41.0
17	37.77437268748431	39.0	37.0	41.0	33.0	41.0
18	37.65577433121228	39.0	36.0	41.0	33.0	41.0
19	37.618384431516795	39.0	36.0	41.0	33.0	41.0
20	37.49892273605396	39.0	36.0	41.0	32.0	41.0
21	37.4441983824669	39.0	36.0	41.0	32.0	41.0
22	37.44033245653289	39.0	36.0	41.0	32.0	41.0
23	37.29187304220648	39.0	36.0	41.0	32.0	41.0
24	37.09210770456555	39.0	35.0	40.0	31.0	41.0
25	37.13419521725368	39.0	35.0	40.0	31.0	41.0
26	37.0613178201504	39.0	35.0	40.0	31.0	41.0
27	36.977046746924835	39.0	35.0	40.0	31.0	41.0
28	36.876807718754435	39.0	35.0	40.0	31.0	41.0
29	36.71707468811735	39.0	35.0	40.0	31.0	41.0
30	36.724881850230844	39.0	35.0	40.0	31.0	41.0
31	36.63779919450781	39.0	35.0	40.0	31.0	41.0
32	36.53223060215453	39.0	35.0	40.0	30.0	41.0
33	36.42110869778762	39.0	35.0	40.0	30.0	41.0
34	36.323199921415394	38.0	35.0	40.0	30.0	41.0
35	36.194969493893325	38.0	35.0	40.0	30.0	41.0
36	36.11733227098591	38.0	35.0	40.0	30.0	41.0
37	36.05536067058862	38.0	35.0	40.0	30.0	41.0
38	35.94107901027057	38.0	35.0	40.0	29.0	41.0
39	35.876064439375256	38.0	35.0	40.0	29.0	41.0
40	35.730130646904094	38.0	35.0	40.0	29.0	41.0
41	35.61110116676308	38.0	34.0	40.0	28.0	41.0
42	35.51473679614936	38.0	34.0	40.0	28.0	41.0
43	35.435432924766154	38.0	34.0	40.0	27.0	41.0
44	35.287977647045984	38.0	34.0	40.0	27.0	41.0
45	35.13739753986531	37.0	34.0	40.0	26.0	41.0
46	34.96399187959092	37.0	34.0	40.0	26.0	41.0
47	34.85429868698224	37.0	33.0	40.0	26.0	41.0
48	34.71459818163958	37.0	33.0	40.0	26.0	41.0
49	34.48930048787942	37.0	33.0	40.0	25.0	41.0
50	34.41082393774353	36.0	33.0	40.0	24.0	41.0
51	33.18782811800788	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	11.0
11	14.0
12	19.0
13	23.0
14	50.0
15	83.0
16	133.0
17	260.0
18	527.0
19	924.0
20	1572.0
21	2354.0
22	3345.0
23	4600.0
24	5778.0
25	6948.0
26	8096.0
27	9725.0
28	12262.0
29	15270.0
30	19606.0
31	25553.0
32	33064.0
33	43767.0
34	63876.0
35	85176.0
36	96071.0
37	128302.0
38	174075.0
39	174686.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.194606040099977	33.59273528994445	20.094738105892755	26.117920564062825
2	23.109221684984885	32.021807227600654	19.61733663679724	25.251634450617217
3	20.919112430556314	33.79618209799064	19.74449089182611	25.54021457962694
4	19.874373778937144	33.92759301906768	19.389332140011568	26.80870106198361
5	17.963239868589078	37.815238864452475	16.81917900917912	27.402342257779328
6	18.973706901256264	41.209657174665196	17.97982995164864	21.8368059724299
7	83.71912552799031	9.041704412743803	3.731895526134838	3.507274533131051
8	85.47680117003745	7.791772628545858	3.7045000600299054	3.0269261413868
9	78.24461640890189	11.991792274696849	6.020126390238046	3.7434649261632162
10	37.07250521168728	36.668449372960346	12.040907652175811	14.218137763176564
11	26.389146593029984	32.50346536274435	22.51361587409	18.593772170135665
12	28.441514499950888	27.622706584734942	25.09664814835027	18.839130766963905
13	24.293448008644305	28.480697656650765	24.960980561225046	22.26487377347988
14	20.65825520350138	30.88047500027286	25.02024645004966	23.441023346176095
15	19.24362318682398	32.235077111142644	26.494253500834965	22.027046201198416
16	22.162495497757064	31.42543739972277	24.380982525840146	22.03108457668002
17	21.978803985985746	31.65005839272656	25.309918031892252	21.061219589395446
18	22.69130439528056	30.504796935200446	24.75043930976523	22.05345935975377
19	24.048853428799074	32.006308597373966	23.356435751628993	20.588402222197967
20	24.59741762259744	31.668285655035415	23.54820401436352	20.18609270800362
21	24.10004256666048	31.28616801824909	24.50791849030244	20.105870924787986
22	24.133113587496315	29.673764748256403	23.0968882679735	23.09623339627378
23	22.999094094148724	31.108806932908394	23.544056493598628	22.348042479344254
24	21.079010270571157	30.122788443697406	26.09358116588992	22.704620119841522
25	21.730607611792056	33.25667696270506	22.717935844402483	22.294779581100403
26	23.68649108828762	31.497800722541776	22.68584713111623	22.129861058054377
27	21.89967365560297	31.48644961307997	23.996245402254942	22.617631329062114
28	21.195249997271368	32.56425928553497	25.565099704216284	20.67539101297737
29	22.432739219174643	32.31016906604381	22.954562818567794	22.30252889621375
30	21.857871012104212	32.668274740507094	23.436766680127917	22.03708756726078
31	23.13061416050905	32.854476593794	21.211294353914496	22.803614891782452
32	23.75961842808963	31.11732026500475	22.635967736654262	22.487093570251364
33	21.964396808591914	31.972364414271837	22.55705569683806	23.506183080298186
34	21.127907357483547	33.67808690147455	23.343010881784743	21.85099485925716
35	23.316706868512675	30.941378068346776	23.521135984108447	22.2207790790321
36	22.647973717815784	33.59120725597843	21.747088549568332	22.013730476637452
37	21.612839851125834	33.53641632376857	23.105074164219992	21.745669660885607
38	22.235077111142644	31.646347453094815	23.56828674648825	22.550288689274293
39	22.04450944652427	30.14767356828675	23.939599000229205	23.86821798495978
40	23.546348544547648	29.385948636229685	24.70536230776787	22.362340511454796
41	21.308979382455988	29.340216762532606	25.824647187871776	23.52615666713963
42	22.050403291821745	30.661420416716687	23.441896508442387	23.846279783019178
43	22.55781971382107	29.841739339234458	24.91393894412853	22.68650200281595
44	22.829045742788225	29.170386701738682	24.23592844435228	23.764639111120815
45	22.950742733652767	29.548247672476833	24.25219109156198	23.24881850230842
46	21.019307800613397	30.69209024132022	25.61246875716266	22.676133200903724
47	21.20158042370199	30.78606433022997	25.256109407231968	22.756245838836076
48	22.09329738815337	29.077831501511664	25.6251296100239	23.203741500311065
49	21.235197170954258	31.62899335305225	24.025168902325884	23.11064057366761
50	21.03829907990526	28.86237871230395	26.413267700636318	23.686054507154473
51	22.06721166544788	27.564095567610046	26.160378079261303	24.208314687680772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17918.0
1	13463.0
2	9008.0
3	5829.5
4	2651.0
5	2363.5
6	2076.0
7	2076.5
8	2077.0
9	2503.5
10	2930.0
11	3531.0
12	4132.0
13	4082.0
14	4032.0
15	4210.5
16	4389.0
17	4222.5
18	4056.0
19	3662.0
20	3268.0
21	3660.5
22	4053.0
23	5032.5
24	6012.0
25	6289.5
26	7976.0
27	9385.0
28	11028.5
29	12672.0
30	15026.0
31	17380.0
32	19713.5
33	22047.0
34	25584.0
35	29121.0
36	30929.0
37	32737.0
38	33123.5
39	33510.0
40	33845.5
41	34181.0
42	37355.0
43	40529.0
44	47676.0
45	54823.0
46	58519.0
47	62215.0
48	64072.0
49	65929.0
50	63386.0
51	60843.0
52	57927.0
53	55011.0
54	51381.0
55	47751.0
56	44808.5
57	41866.0
58	42445.0
59	43024.0
60	42627.5
61	42231.0
62	37527.0
63	32823.0
64	28764.0
65	24705.0
66	22131.5
67	19558.0
68	18233.5
69	16909.0
70	16101.0
71	15293.0
72	13374.5
73	11456.0
74	9063.0
75	5909.5
76	5149.0
77	3758.0
78	2367.0
79	1960.5
80	1554.0
81	1042.0
82	530.0
83	471.0
84	412.0
85	264.0
86	116.0
87	98.0
88	80.0
89	63.0
90	46.0
91	60.5
92	75.0
93	58.0
94	41.0
95	21.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	916210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.392818506080797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.00027152370426	17.466459236553316
2	8.725395183998279	3.907723814982098
3	3.2950901552391985	2.2135906742233487
4	1.674566342507969	1.4999304073668995
5	1.102240822060886	1.2341139339201361
6	0.7752181446220843	1.0415591529085817
7	0.5675836608810788	0.8896858533588837
8	0.43263028394250147	0.775024914284691
9	0.3424746609345552	0.6902075632715148
>10	3.1269616611802635	15.98356228753831
>50	1.0009665552191533	16.28698688068581
>100	0.9486251765845536	34.15772073624375
>500	0.003987914562625553	0.5620359471735064
>1k	0.0034894252422973593	1.5902212717842645
>5k	0.0	0.0
>10k+	4.984893203281942E-4	1.701177325704913
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15240	1.6633741172875214	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3265	0.35635934993069274	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2786	0.3040787592364196	No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT	2244	0.24492201569509173	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2164	0.2361903930321651	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1521	0.1660099758788924	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1211	0.13217493806005173	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1055	0.11514827386734483	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2743584985974833E-4	0.0	0.0	0.2956745724233527	0.0
2	3.2743584985974833E-4	0.0	0.0	0.3796072952707349	0.0
3	3.2743584985974833E-4	0.0	0.0	0.4874428351578787	0.0
4	3.2743584985974833E-4	0.0	0.0	0.9582955872561967	0.0
5	3.2743584985974833E-4	0.0	0.0	1.03644361008939	0.0
6	3.2743584985974833E-4	0.0	0.0	1.5361107169753658	0.0
7	3.2743584985974833E-4	0.0	0.0	1.942349461368027	0.0
8	3.2743584985974833E-4	0.0	0.0	2.3460778642450966	0.0
9	3.2743584985974833E-4	0.0	0.0	2.8944237674768885	0.0
10	3.2743584985974833E-4	0.0	0.0	3.7537245827921546	0.0
11	3.2743584985974833E-4	0.0	0.0	4.466224992086967	0.0
12	3.2743584985974833E-4	0.0	0.0	4.8271684439156965	0.0
13	3.2743584985974833E-4	0.0	0.0	4.982482182032504	0.0
14	3.2743584985974833E-4	0.0	0.0	5.134630706934	0.0
15	3.2743584985974833E-4	0.0	0.0	5.268879405376497	0.0
16	3.2743584985974833E-4	0.0	0.0	5.54250663057596	0.0
17	3.2743584985974833E-4	0.0	0.0	5.890025212560439	0.0
18	3.2743584985974833E-4	0.0	0.0	6.346689077831502	0.0
19	3.2743584985974833E-4	0.0	0.0	6.53005315375296	0.0
20	3.2743584985974833E-4	0.0	0.0	6.741467567479072	0.0
21	3.2743584985974833E-4	0.0	0.0	6.966306851049432	0.0
22	3.2743584985974833E-4	0.0	0.0	7.2241080101723405	0.0
23	3.2743584985974833E-4	0.0	0.0	7.482127459861822	0.0
24	3.2743584985974833E-4	0.0	0.0	7.665382390499994	0.0
25	3.2743584985974833E-4	0.0	0.0	7.8230973248491065	0.0
26	3.2743584985974833E-4	0.0	0.0	7.971753200685432	0.0
27	3.2743584985974833E-4	0.0	0.0	8.140382663363203	0.0
28	3.2743584985974833E-4	0.0	0.0	8.306283493958809	0.0
29	3.2743584985974833E-4	0.0	0.0	8.481789109483634	0.0
30	3.2743584985974833E-4	0.0	0.0	8.708156427020006	0.0
31	3.2743584985974833E-4	0.0	0.0	8.864125036836533	0.0
32	3.2743584985974833E-4	0.0	0.0	9.047816548607852	0.0
33	3.2743584985974833E-4	0.0	0.0	9.219829515067506	0.0
34	3.2743584985974833E-4	0.0	0.0	9.386057781512973	0.0
35	3.2743584985974833E-4	0.0	0.0	9.582082710295674	0.0
36	3.2743584985974833E-4	0.0	0.0	9.751148754106591	0.0
37	3.2743584985974833E-4	0.0	0.0	9.923489156416105	0.0
38	3.2743584985974833E-4	0.0	0.0	10.113947675751193	0.0
39	3.2743584985974833E-4	0.0	0.0	10.308117134718023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	30	2.1650176E-6	45.000004	26
CGTATAG	35	1.2115743E-7	45.000004	1
CGAAATA	30	2.1650176E-6	45.000004	5
CTATCGC	35	1.2115743E-7	45.000004	17
GCGAAAT	30	2.1650176E-6	45.000004	4
TCCGAAC	30	2.1650176E-6	45.000004	23
ACTCTCG	30	2.1650176E-6	45.000004	37
TTCACGC	35	1.2115743E-7	45.000004	20
TAAACGG	120	0.0	45.000004	2
GCACGAG	70	0.0	45.000004	1
ATATGCG	60	0.0	45.000004	1
TAACGCG	70	0.0	45.000004	1
CCGTCTA	30	2.1650176E-6	45.000004	36
CATACGC	30	2.1650176E-6	45.000004	17
GTTCACG	35	1.2115743E-7	45.000004	19
ATAACGG	130	0.0	45.000004	2
ACCGTCA	30	2.1650176E-6	45.000004	39
GCGTGCA	35	1.2115743E-7	45.000004	19
GGCGATA	70	0.0	45.000004	8
CTCTAGG	60	0.0	45.000004	2
>>END_MODULE