FastQCFastQC Report
Sat 18 Jun 2016
SRR3553339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906079
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA149691.6520634514209025No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32000.3531700878179497No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27120.2993116494257123No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA20930.2309953105634277No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT20610.22746360968524817No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16640.1836484456653338No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11410.12592720943758767No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG10090.11135894331509724No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA207.0324936E-445.00000438
TCGCACT207.0324936E-445.00000430
GTCGAAC406.8121153E-945.00000413
CGTTCCA207.0324936E-445.00000417
AGTATCC406.8121153E-945.00000421
TCAATCG207.0324936E-445.00000416
CCGTAGA207.0324936E-445.00000424
TAACCGT207.0324936E-445.00000412
TGCGATA207.0324936E-445.00000426
CTACGCT207.0324936E-445.00000442
CTACGCC207.0324936E-445.00000441
TTGCGTA406.8121153E-945.00000422
TCGTACG207.0324936E-445.0000042
TTCGGTA207.0324936E-445.00000425
CGTAAGG406.8121153E-945.0000042
GCTAACG406.8121153E-945.0000041
CCACGCG207.0324936E-445.0000041
TCGACCT406.8121153E-945.00000435
CGATACG207.0324936E-445.0000041
GCGTAAT406.8121153E-945.00000424