##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553339_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	906079
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.668789366048657	31.0	31.0	34.0	30.0	34.0
2	31.880329419399413	33.0	31.0	34.0	30.0	34.0
3	32.04415067560334	33.0	31.0	34.0	30.0	34.0
4	35.77194814138723	37.0	35.0	37.0	35.0	37.0
5	35.5292617972605	37.0	35.0	37.0	33.0	37.0
6	32.75926823157804	37.0	35.0	37.0	26.0	37.0
7	34.238683381912615	37.0	35.0	37.0	28.0	37.0
8	35.33082104319822	37.0	35.0	37.0	32.0	37.0
9	37.18110120640695	39.0	37.0	39.0	34.0	39.0
10	36.734811202996646	39.0	37.0	39.0	32.0	39.0
11	36.78774146625184	39.0	37.0	39.0	32.0	39.0
12	36.72565747578302	39.0	35.0	39.0	32.0	39.0
13	36.72062259471856	39.0	35.0	39.0	32.0	39.0
14	37.89814464301678	40.0	37.0	41.0	33.0	41.0
15	37.93491075281515	40.0	37.0	41.0	33.0	41.0
16	37.8823347633043	40.0	37.0	41.0	33.0	41.0
17	37.857836899431504	40.0	37.0	41.0	33.0	41.0
18	37.737669673394926	39.0	37.0	41.0	33.0	41.0
19	37.70179752538134	39.0	36.0	41.0	33.0	41.0
20	37.568868718952764	39.0	36.0	41.0	32.0	41.0
21	37.50064950186463	39.0	36.0	41.0	32.0	41.0
22	37.467525458596874	39.0	36.0	41.0	32.0	41.0
23	37.32284160652658	39.0	36.0	41.0	32.0	41.0
24	37.185895490349075	39.0	35.0	41.0	32.0	41.0
25	37.193491958206735	39.0	35.0	41.0	32.0	41.0
26	37.1287194604444	39.0	35.0	41.0	32.0	41.0
27	37.02477708897348	39.0	35.0	41.0	31.0	41.0
28	36.95395213883116	39.0	35.0	40.0	31.0	41.0
29	36.746911693130514	39.0	35.0	40.0	31.0	41.0
30	36.76167972108392	39.0	35.0	40.0	31.0	41.0
31	36.370228203059554	39.0	35.0	40.0	30.0	41.0
32	36.44537507215154	39.0	35.0	40.0	30.0	41.0
33	36.40119680513509	39.0	35.0	40.0	30.0	41.0
34	36.33568927212749	38.0	35.0	40.0	30.0	41.0
35	36.29610552722224	38.0	35.0	40.0	30.0	41.0
36	36.19103852975292	38.0	35.0	40.0	30.0	41.0
37	36.112756172475024	38.0	35.0	40.0	30.0	41.0
38	35.9991733612632	38.0	35.0	40.0	30.0	41.0
39	35.90704784019936	38.0	35.0	40.0	29.0	41.0
40	35.785699701681644	38.0	35.0	40.0	29.0	41.0
41	35.72673574820739	38.0	35.0	40.0	29.0	41.0
42	35.618064208529276	38.0	34.0	40.0	28.0	41.0
43	35.55580915129917	38.0	34.0	40.0	28.0	41.0
44	35.35393933641548	38.0	34.0	40.0	27.0	41.0
45	35.19189386355936	38.0	34.0	40.0	27.0	41.0
46	34.93334687152003	37.0	34.0	40.0	26.0	41.0
47	34.89836206335209	37.0	34.0	40.0	26.0	41.0
48	34.85307351787206	37.0	34.0	40.0	26.0	41.0
49	34.67492238535492	37.0	33.0	40.0	26.0	41.0
50	34.56644840019469	37.0	33.0	40.0	26.0	41.0
51	33.41088359845003	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	9.0
10	9.0
11	17.0
12	22.0
13	22.0
14	53.0
15	58.0
16	138.0
17	278.0
18	512.0
19	931.0
20	1650.0
21	2339.0
22	3268.0
23	4531.0
24	5653.0
25	6648.0
26	7796.0
27	9619.0
28	12097.0
29	14836.0
30	19185.0
31	25043.0
32	32480.0
33	43868.0
34	63484.0
35	85483.0
36	97397.0
37	129647.0
38	170515.0
39	168464.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.208392424943078	33.54641261965016	20.132681587367106	26.112513368039654
2	23.16519861954642	31.932756415279464	19.58681307038349	25.315231894790628
3	20.9695843298432	33.75147200188946	19.734261582047484	25.544682086219854
4	19.911288088566227	33.961166741531365	19.301628224470495	26.82591694543191
5	18.01873788047179	37.6957196889013	16.841136368903815	27.444406061723093
6	18.25657586148669	43.60855951854088	17.170467475794055	20.964397144178378
7	83.80461306354083	8.965112313606209	3.7379742825956677	3.4923003402573065
8	85.28108476192473	7.857924088296936	3.681577434197239	3.179413715581092
9	78.2270640860234	12.01705370061551	6.006098805954006	3.7497834074070804
10	37.02866968553515	36.64570087155756	12.169358300987	14.156271141920296
11	26.463365777156294	32.4766383505191	22.506315674461057	18.553680197863542
12	28.492769394280188	27.691845854500546	25.062604916348352	18.75277983487091
13	24.211465004707094	28.544972347885782	24.928841745587306	22.314720901819822
14	20.605929505043157	30.958669166816584	25.016361707974692	23.41903962016557
15	19.221944223406567	32.31903619883034	26.557838775647596	21.901180802115487
16	22.175439448436617	31.494494409427876	24.343351959376612	21.9867141827589
17	22.08615363561014	31.633665497158635	25.243714952007494	21.03646591522373
18	22.676389144875888	30.630883179060547	24.749276829062367	21.943450847001202
19	24.154295596741566	32.06232569124767	23.179766885668908	20.603611826341854
20	24.66992392495577	31.68597881641667	23.506449216900513	20.137648041727047
21	24.109266410544773	31.361945260843697	24.502940692809347	20.025847635802176
22	24.06335429912844	29.76782377695543	23.1134371285506	23.055384795365523
23	22.990600157381422	30.982949610354066	23.688111080821873	22.338339151442643
24	21.03977688479702	30.08335917729028	26.149154764650767	22.727709173261935
25	21.747331082609794	33.25957228895052	22.754859123762937	22.238237504676743
26	23.587126508836427	31.385122047856758	22.756293877244698	22.271457566062118
27	21.896324713407992	31.540627252149093	23.951222796246245	22.61182523819667
28	21.164379706405292	32.58435522730358	25.57150093976353	20.679764126527598
29	22.5328034310474	32.26274971608436	22.885200959298253	22.319245893569985
30	21.874693045529142	32.67342030882517	23.411424390146994	22.040462255498696
31	23.138821228612517	32.89602782980292	21.176188831216706	22.788962110367862
32	23.790640771941522	31.143973097268564	22.68146596488827	22.38392016590165
33	22.0619835577251	31.93794360094429	22.601340501214572	23.398732340116037
34	21.125751728050204	33.587579008011446	23.4129695092812	21.873699754657157
35	23.232080204926945	30.95624112246283	23.520024192151016	22.291654480459208
36	22.70563604277331	33.52080778828336	21.829001665417696	21.94455450352563
37	21.698439098577495	33.47953103426964	23.133744408600133	21.68828545855273
38	22.213405232877044	31.646799009799366	23.56339789356116	22.57639786376243
39	22.112199929586716	30.20774126759366	23.981683716320543	23.69837508649908
40	23.61538011586186	29.356270258995075	24.77775116739269	22.250598457750375
41	21.203338781717708	29.515638261122927	25.91087532102609	23.37014763613327
42	21.951507539629546	30.747318942388024	23.52543210912073	23.775741408861702
43	22.472874881770792	29.915272288619427	24.950142316508824	22.661710513100957
44	22.789845035587405	29.244359487417764	24.302627033625104	23.663168443369727
45	22.988172113027673	29.608014312217808	24.27271794181302	23.1310956329415
46	21.00423914471034	30.80139811208515	25.58143384848341	22.6129288947211
47	21.125089534135544	30.832852323031435	25.33266966787664	22.709388474956377
48	22.06948842209123	29.09635914749155	25.690916575706975	23.14323585471024
49	21.18314186732062	31.791488380152284	24.031568991224827	22.99380076130227
50	21.047392114815597	28.919553372277697	26.46104809845499	23.57200641445172
51	22.05094699248079	27.56845705506915	26.258306394916996	24.122289557533065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17803.0
1	13405.5
2	9008.0
3	5851.0
4	2694.0
5	2321.0
6	1948.0
7	2004.5
8	2061.0
9	2427.0
10	2793.0
11	3386.5
12	3980.0
13	3968.5
14	3957.0
15	4130.5
16	4304.0
17	4161.0
18	4018.0
19	3663.0
20	3308.0
21	3608.0
22	3908.0
23	4920.5
24	5933.0
25	6188.0
26	7905.0
27	9367.0
28	11072.5
29	12778.0
30	15074.5
31	17371.0
32	19604.0
33	21837.0
34	25650.5
35	29464.0
36	31031.5
37	32599.0
38	33054.5
39	33510.0
40	33883.0
41	34256.0
42	37437.5
43	40619.0
44	47389.0
45	54159.0
46	58105.5
47	62052.0
48	63443.0
49	64834.0
50	62123.0
51	59412.0
52	56530.5
53	53649.0
54	50340.0
55	47031.0
56	44152.0
57	41273.0
58	41984.5
59	42696.0
60	42163.0
61	41630.0
62	37056.0
63	32482.0
64	28464.0
65	24446.0
66	21923.0
67	19400.0
68	17914.5
69	16429.0
70	15729.5
71	15030.0
72	13078.5
73	11127.0
74	8816.0
75	5750.0
76	4995.0
77	3673.0
78	2351.0
79	1875.0
80	1399.0
81	933.0
82	467.0
83	448.0
84	429.0
85	262.5
86	96.0
87	85.5
88	75.0
89	58.0
90	41.0
91	62.5
92	84.0
93	55.0
94	26.0
95	14.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	906079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.561354008915885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68166087333489	17.300420969549425
2	8.860580853755053	3.9981340273237977
3	3.37088823280165	2.281554082341807
4	1.8799484373758673	1.696567288565807
5	1.278431508836607	1.4421572923507584
6	0.8883265676613983	1.2025110101120386
7	0.6848452807032527	1.0815725773497016
8	0.4998239724052324	0.9021364466861642
9	0.4327429892467842	0.8786940997745863
>10	3.524116369795448	17.238595583993757
>50	1.003174159833714	16.410749148357706
>100	0.8880151271360172	31.8518979437196
>500	0.0034746259865579207	0.4842402317299892
>1k	0.0034746259865579207	1.5544066642951553
>5k	0.0	0.0
>10k+	4.963751409368458E-4	1.6763626338497244
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14969	1.6520634514209025	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3200	0.3531700878179497	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2712	0.2993116494257123	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2093	0.2309953105634277	No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT	2061	0.22746360968524817	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1664	0.1836484456653338	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1141	0.12592720943758767	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1009	0.11135894331509724	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2073130488621854E-4	0.0	0.0	0.2692921919611866	0.0
2	2.2073130488621854E-4	0.0	0.0	0.35570849782414116	0.0
3	2.2073130488621854E-4	0.0	0.0	0.45779672633401725	0.0
4	2.2073130488621854E-4	0.0	0.0	0.910075170045879	0.0
5	2.2073130488621854E-4	0.0	0.0	0.9900902680671332	0.0
6	2.2073130488621854E-4	0.0	0.0	1.4683046401031257	0.0
7	2.2073130488621854E-4	0.0	0.0	1.859661243666391	0.0
8	2.2073130488621854E-4	0.0	0.0	2.256536129851812	0.0
9	2.2073130488621854E-4	0.0	0.0	2.7845254111396467	0.0
10	2.2073130488621854E-4	0.0	0.0	3.614916580121601	0.0
11	2.2073130488621854E-4	0.0	0.0	4.302273863537286	0.0
12	2.2073130488621854E-4	0.0	0.0	4.646283602202457	0.0
13	2.2073130488621854E-4	0.0	0.0	4.792518091689577	0.0
14	2.2073130488621854E-4	0.0	0.0	4.950340974683223	0.0
15	2.2073130488621854E-4	0.0	0.0	5.075716355858595	0.0
16	2.2073130488621854E-4	0.0	0.0	5.336620758234106	0.0
17	2.2073130488621854E-4	0.0	0.0	5.676657333411325	0.0
18	3.310969573293278E-4	0.0	0.0	6.119665062317965	0.0
19	3.310969573293278E-4	0.0	0.0	6.299119613190461	0.0
20	3.310969573293278E-4	0.0	0.0	6.500978391508908	0.0
21	3.310969573293278E-4	0.0	0.0	6.726013956840408	0.0
22	3.310969573293278E-4	0.0	0.0	6.978530569630242	0.0
23	3.310969573293278E-4	0.0	0.0	7.230274622852974	0.0
24	3.310969573293278E-4	0.0	0.0	7.406749301109506	0.0
25	3.310969573293278E-4	0.0	0.0	7.561813042792075	0.0
26	3.310969573293278E-4	0.0	0.0	7.704626197053458	0.0
27	3.310969573293278E-4	0.0	0.0	7.856599700467619	0.0
28	3.310969573293278E-4	0.0	0.0	8.020492694345636	0.0
29	3.310969573293278E-4	0.0	0.0	8.192994209114216	0.0
30	3.310969573293278E-4	0.0	0.0	8.416484655311512	0.0
31	3.310969573293278E-4	0.0	0.0	8.568789255683003	0.0
32	3.310969573293278E-4	0.0	0.0	8.744601740024876	0.0
33	3.310969573293278E-4	0.0	0.0	8.908825830860224	0.0
34	3.310969573293278E-4	0.0	0.0	9.076802353878636	0.0
35	3.310969573293278E-4	0.0	0.0	9.265306888251466	0.0
36	3.310969573293278E-4	0.0	0.0	9.439684619111578	0.0
37	3.310969573293278E-4	0.0	0.0	9.607330045172661	0.0
38	3.310969573293278E-4	0.0	0.0	9.783473626471864	0.0
39	3.310969573293278E-4	0.0	0.0	9.970764138667821	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	20	7.0324936E-4	45.000004	38
TCGCACT	20	7.0324936E-4	45.000004	30
GTCGAAC	40	6.8121153E-9	45.000004	13
CGTTCCA	20	7.0324936E-4	45.000004	17
AGTATCC	40	6.8121153E-9	45.000004	21
TCAATCG	20	7.0324936E-4	45.000004	16
CCGTAGA	20	7.0324936E-4	45.000004	24
TAACCGT	20	7.0324936E-4	45.000004	12
TGCGATA	20	7.0324936E-4	45.000004	26
CTACGCT	20	7.0324936E-4	45.000004	42
CTACGCC	20	7.0324936E-4	45.000004	41
TTGCGTA	40	6.8121153E-9	45.000004	22
TCGTACG	20	7.0324936E-4	45.000004	2
TTCGGTA	20	7.0324936E-4	45.000004	25
CGTAAGG	40	6.8121153E-9	45.000004	2
GCTAACG	40	6.8121153E-9	45.000004	1
CCACGCG	20	7.0324936E-4	45.000004	1
TCGACCT	40	6.8121153E-9	45.000004	35
CGATACG	20	7.0324936E-4	45.000004	1
GCGTAAT	40	6.8121153E-9	45.000004	24
>>END_MODULE