FastQCFastQC Report
Sat 18 Jun 2016
SRR3553338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369425
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123620.9027146430071015No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT28320.20680212497946218TruSeq Adapter, Index 13 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26280.1919053617394162No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20580.15028205268634645No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGT19790.14451320809828944No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCC17110.12494295050842508No Hit
AATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT16010.11691038209467478No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA15400.11245595779250415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGA453.8562575E-1045.00000413
ACGAACG650.045.0000041
TCACGGT207.0337596E-445.038
TCGCAAA207.0337596E-445.012
GTCGATT207.0337596E-445.035
CGAGTTA351.2121563E-745.034
CGAAATT207.0337596E-445.042
GCGAACT207.0337596E-445.045
TAGTTCG207.0337596E-445.021
TACGCGA351.2121563E-745.021
GTATCGG850.045.02
ACGCTAC406.8175723E-945.039
CCGAACG207.0337596E-445.01
GACGATA207.0337596E-445.042
CGGTATC207.0337596E-445.023
TTCGCTA207.0337596E-445.024
TAATGCG351.2121563E-745.01
TACGTCC207.0337596E-445.020
ACGATAG302.1658088E-644.9999961
TGCGACA302.1658088E-644.99999630