Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553338_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1369425 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 12362 | 0.9027146430071015 | No Hit |
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT | 2832 | 0.20680212497946218 | TruSeq Adapter, Index 13 (95% over 22bp) |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2628 | 0.1919053617394162 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2058 | 0.15028205268634645 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGT | 1979 | 0.14451320809828944 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCC | 1711 | 0.12494295050842508 | No Hit |
AATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT | 1601 | 0.11691038209467478 | No Hit |
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 1540 | 0.11245595779250415 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TAAGCGA | 45 | 3.8562575E-10 | 45.000004 | 13 |
ACGAACG | 65 | 0.0 | 45.000004 | 1 |
TCACGGT | 20 | 7.0337596E-4 | 45.0 | 38 |
TCGCAAA | 20 | 7.0337596E-4 | 45.0 | 12 |
GTCGATT | 20 | 7.0337596E-4 | 45.0 | 35 |
CGAGTTA | 35 | 1.2121563E-7 | 45.0 | 34 |
CGAAATT | 20 | 7.0337596E-4 | 45.0 | 42 |
GCGAACT | 20 | 7.0337596E-4 | 45.0 | 45 |
TAGTTCG | 20 | 7.0337596E-4 | 45.0 | 21 |
TACGCGA | 35 | 1.2121563E-7 | 45.0 | 21 |
GTATCGG | 85 | 0.0 | 45.0 | 2 |
ACGCTAC | 40 | 6.8175723E-9 | 45.0 | 39 |
CCGAACG | 20 | 7.0337596E-4 | 45.0 | 1 |
GACGATA | 20 | 7.0337596E-4 | 45.0 | 42 |
CGGTATC | 20 | 7.0337596E-4 | 45.0 | 23 |
TTCGCTA | 20 | 7.0337596E-4 | 45.0 | 24 |
TAATGCG | 35 | 1.2121563E-7 | 45.0 | 1 |
TACGTCC | 20 | 7.0337596E-4 | 45.0 | 20 |
ACGATAG | 30 | 2.1658088E-6 | 44.999996 | 1 |
TGCGACA | 30 | 2.1658088E-6 | 44.999996 | 30 |