##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553338_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1369425
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.699283275827447	31.0	31.0	34.0	30.0	34.0
2	31.900977417529255	33.0	31.0	34.0	30.0	34.0
3	32.06430801248699	33.0	31.0	34.0	30.0	34.0
4	35.776716505102506	37.0	35.0	37.0	35.0	37.0
5	35.519261003705935	37.0	35.0	37.0	33.0	37.0
6	34.17961188089892	37.0	35.0	37.0	32.0	37.0
7	34.98793362177556	37.0	35.0	37.0	32.0	37.0
8	35.680928126768535	37.0	35.0	37.0	33.0	37.0
9	37.34099932453402	39.0	37.0	39.0	35.0	39.0
10	36.78239407050405	39.0	37.0	39.0	32.0	39.0
11	36.768896434634975	39.0	37.0	39.0	32.0	39.0
12	36.757467550249196	39.0	35.0	39.0	32.0	39.0
13	36.748625152892636	39.0	37.0	39.0	33.0	39.0
14	37.94121182248024	40.0	37.0	41.0	33.0	41.0
15	37.947193895248006	40.0	37.0	41.0	33.0	41.0
16	37.85900432663344	40.0	37.0	41.0	33.0	41.0
17	37.83832922576994	40.0	37.0	41.0	33.0	41.0
18	37.75074136955291	39.0	37.0	41.0	33.0	41.0
19	37.772403746097815	40.0	37.0	41.0	33.0	41.0
20	37.665694725888606	39.0	36.0	41.0	33.0	41.0
21	37.648950471913395	39.0	36.0	41.0	33.0	41.0
22	37.60612957993318	39.0	36.0	41.0	32.0	41.0
23	37.4743195136645	39.0	36.0	41.0	32.0	41.0
24	37.30702594154481	39.0	36.0	41.0	32.0	41.0
25	37.31170600799606	39.0	36.0	41.0	32.0	41.0
26	37.24515763915512	39.0	36.0	41.0	32.0	41.0
27	37.211224419007976	39.0	36.0	41.0	32.0	41.0
28	37.072513646238384	39.0	35.0	41.0	31.0	41.0
29	36.92412070759625	39.0	35.0	40.0	31.0	41.0
30	36.96331964145536	39.0	35.0	40.0	31.0	41.0
31	36.912520218339814	39.0	35.0	40.0	31.0	41.0
32	36.850429194735014	39.0	35.0	40.0	31.0	41.0
33	36.77486901436735	39.0	35.0	40.0	31.0	41.0
34	36.71685780528324	39.0	35.0	40.0	31.0	41.0
35	36.61120616317067	39.0	35.0	40.0	30.0	41.0
36	36.54041732844077	39.0	35.0	40.0	30.0	41.0
37	36.46213045621337	39.0	35.0	40.0	30.0	41.0
38	36.39578801321723	39.0	35.0	40.0	30.0	41.0
39	36.35232451576392	39.0	35.0	40.0	30.0	41.0
40	36.21708819395001	38.0	35.0	40.0	30.0	41.0
41	36.1284188619311	38.0	35.0	40.0	30.0	41.0
42	36.003399236906	38.0	35.0	40.0	29.0	41.0
43	35.945164576373294	38.0	35.0	40.0	29.0	41.0
44	35.82993957317852	38.0	35.0	40.0	29.0	41.0
45	35.69352903590923	38.0	34.0	40.0	28.0	41.0
46	35.51044635522208	38.0	34.0	40.0	28.0	41.0
47	35.42552239078445	38.0	34.0	40.0	28.0	41.0
48	35.266912025120035	38.0	34.0	40.0	27.0	41.0
49	35.11065593223433	37.0	34.0	40.0	27.0	41.0
50	35.02580791207989	37.0	34.0	40.0	27.0	41.0
51	33.77496175402085	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	10.0
11	16.0
12	21.0
13	26.0
14	45.0
15	70.0
16	150.0
17	289.0
18	573.0
19	1081.0
20	1749.0
21	2815.0
22	4036.0
23	5277.0
24	6797.0
25	8223.0
26	10025.0
27	12813.0
28	16341.0
29	21250.0
30	27567.0
31	35898.0
32	46850.0
33	63356.0
34	91002.0
35	116932.0
36	140383.0
37	192461.0
38	273486.0
39	289828.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.082443361264765	31.532285448272084	22.134545520930317	26.25072566953283
2	23.355203826423498	30.711722073132886	21.531591726454533	24.401482373989083
3	20.627489639812328	31.70776055643792	20.982894280446175	26.681855523303575
4	20.62544498603428	31.957792504153204	20.063165197071765	27.353597312740746
5	18.22304982018	35.839129561677346	17.49668656552933	28.44113405261332
6	19.520893805794405	39.983058582981904	18.25277032331088	22.24327728791281
7	86.30213410738084	6.438614747065374	3.7374810595687973	3.521770085984994
8	87.79940485970388	5.477627471383975	3.191266407433777	3.531701261478358
9	80.68203808167662	9.391459919309199	5.857275863957501	4.0692261350566845
10	36.11917410592037	38.25707870091462	11.784654143162276	13.839093050002738
11	24.13589645289081	29.807912079887544	27.48803329864724	18.568158168574403
12	26.55136279825474	25.10352885335086	28.720083246618106	19.62502510177629
13	24.902276502911803	26.173613012760832	26.720265804991143	22.203844679336218
14	21.092356280920825	27.8278839658981	28.19935374335944	22.88040600982164
15	19.757927597349255	29.784836701535315	29.222556912572795	21.234678788542634
16	22.715665333990543	29.385106887927414	25.924676415283788	21.974551362798255
17	22.76283841758402	29.185022911075816	26.766197491647954	21.285941179692205
18	23.10261606148566	29.022326889022764	26.126366905818138	21.74869014367344
19	23.10728955583548	30.16988882195082	25.73897803822772	20.98384358398598
20	24.28187012797342	28.748927469558396	26.350256494514117	20.618945907954068
21	24.644285010131988	28.63464592803549	25.942348065794036	20.778720996038484
22	24.06579403764354	28.059221936214108	25.54502802271026	22.3299560034321
23	22.612191248151596	28.735783266699528	25.8618763349581	22.79014915019077
24	20.815086623948005	29.47251583693886	27.056830421527284	22.655567117585846
25	21.46499443196962	31.013381528743817	24.89468207459335	22.626941964693213
26	23.312777260529057	30.00522116946894	24.294284097340125	22.387717472661883
27	21.09301349106377	29.3946729466747	26.453438486956205	23.05887507530533
28	22.34193183270351	29.31551563612465	27.143655183745004	21.19889734742684
29	23.077130912609306	28.904613250086715	25.834492579002138	22.18376325830184
30	22.217134928893515	29.46068605436588	25.35480219800281	22.96737681873779
31	22.9793526480092	30.841557588038775	23.825255125326322	22.3538346386257
32	23.023531774284827	28.443105683042152	25.211676433539626	23.321686109133395
33	22.24795078226263	29.80856929003049	25.05065994851854	22.892819979188346
34	21.807546963141462	28.724975811015575	27.19170454752907	22.275772678313892
35	23.924493856910747	28.143052741113973	26.12570969567519	21.80674370630009
36	23.79173740803622	29.360059879146355	25.71473428628804	21.133468426529383
37	22.221954469941764	30.34120159921135	26.2863610639502	21.15048286689669
38	22.088759880971942	28.36467860598426	27.901637548606168	21.64492396443763
39	21.820691166000326	28.489475509794254	26.903846504919947	22.785986819285466
40	22.204063749383867	28.733300472826183	26.695802983003812	22.36683279478613
41	21.256074629862898	28.29311572375267	28.594483085966736	21.856326560417692
42	21.45557441992077	28.758493528305674	26.30045457034887	23.485477481424684
43	21.71816638370119	29.500702849736204	25.656242583566097	23.124888182996514
44	22.541139529364514	26.9229055990653	26.101173850338643	24.43478102123154
45	22.65585921098271	28.55249466016759	26.480530149515307	22.311115979334392
46	20.84429596363437	29.42395530971028	28.24776822388959	21.48398050276576
47	21.612501597385762	28.155393687131458	27.664457710352885	22.56764700512989
48	20.932800262884058	27.32774704711832	29.118790733336986	22.620661956660644
49	21.49201307117951	28.65005385472005	27.130876097632218	22.727056976468226
50	21.15982985559633	26.891505558902455	28.4065940084342	23.54207057706702
51	21.867134016101648	26.541285576062947	27.87637146977746	23.715208938057945
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14454.0
1	10831.5
2	7209.0
3	4603.5
4	1998.0
5	1812.0
6	1626.0
7	1660.0
8	1694.0
9	1981.5
10	2269.0
11	2426.0
12	2583.0
13	2954.5
14	3326.0
15	3955.0
16	4584.0
17	4305.5
18	4027.0
19	4307.0
20	4587.0
21	4586.0
22	4585.0
23	6218.0
24	7851.0
25	9246.5
26	13010.5
27	15379.0
28	18450.5
29	21522.0
30	26045.0
31	30568.0
32	36475.5
33	42383.0
34	46064.5
35	49746.0
36	52279.5
37	54813.0
38	59422.5
39	64032.0
40	62047.0
41	60062.0
42	63914.0
43	67766.0
44	74409.0
45	81052.0
46	89494.5
47	97937.0
48	101171.0
49	104405.0
50	100340.5
51	96276.0
52	91591.0
53	86906.0
54	81058.5
55	75211.0
56	71301.0
57	67391.0
58	63498.5
59	59606.0
60	56121.0
61	52636.0
62	48149.5
63	43663.0
64	39268.5
65	34874.0
66	32223.5
67	29573.0
68	25372.5
69	21172.0
70	17454.5
71	13737.0
72	12400.0
73	11063.0
74	8868.5
75	5262.0
76	3850.0
77	3455.0
78	3060.0
79	2170.5
80	1281.0
81	956.0
82	631.0
83	446.5
84	262.0
85	251.5
86	241.0
87	189.0
88	137.0
89	89.5
90	42.0
91	30.5
92	19.0
93	17.5
94	16.0
95	8.5
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1369425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.676368538261034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.93579437088441	16.676886325671592
2	9.07405985667975	3.9338533118326073
3	3.431977368424473	2.2317841875882043
4	1.7928502163888496	1.5544992809738296
5	1.0960803982404537	1.1879521329911995
6	0.7560226564874877	0.9832695435178744
7	0.5630927093483512	0.8544063562329935
8	0.419210192698746	0.7269563705546754
9	0.3173742488933603	0.6191569065209637
>10	3.458776026631727	17.44907984983185
>50	1.2056159532956148	18.92414514792542
>100	0.9429243603529094	32.271570730859054
>500	0.0024195273176210582	0.3148292422817943
>1k	0.003456467596601512	1.345404260745878
>5k	0.0	0.0
>10k+	3.4564675966015115E-4	0.926206352472047
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12362	0.9027146430071015	No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	2832	0.20680212497946218	TruSeq Adapter, Index 13 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2628	0.1919053617394162	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2058	0.15028205268634645	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGT	1979	0.14451320809828944	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCC	1711	0.12494295050842508	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT	1601	0.11691038209467478	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1540	0.11245595779250415	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4604669843182357E-4	0.0	0.0	0.25448637201745256	0.0
2	1.4604669843182357E-4	0.0	0.0	0.3443050915530241	0.0
3	1.4604669843182357E-4	0.0	0.0	0.4589517498220056	0.0
4	1.4604669843182357E-4	0.0	0.0	0.9799003231283203	0.0
5	1.4604669843182357E-4	0.0	0.0	1.0649725249648576	0.0
6	1.4604669843182357E-4	0.0	0.0	1.5606550194424667	0.0
7	1.4604669843182357E-4	0.0	0.0	1.9498694707632767	0.0
8	1.4604669843182357E-4	0.0	0.0	2.3246252989393357	0.0
9	1.4604669843182357E-4	0.0	0.0	2.9556930828632457	0.0
10	1.4604669843182357E-4	0.0	0.0	3.7820253025905033	0.0
11	1.4604669843182357E-4	0.0	0.0	4.561622578819578	0.0
12	1.4604669843182357E-4	0.0	0.0	4.929076072074046	0.0
13	1.4604669843182357E-4	0.0	0.0	5.091918140825529	0.0
14	1.4604669843182357E-4	0.0	0.0	5.259725797323695	0.0
15	1.4604669843182357E-4	0.0	0.0	5.403362725231393	0.0
16	1.4604669843182357E-4	0.0	0.0	5.684356573014221	0.0
17	1.4604669843182357E-4	0.0	0.0	6.07787940193877	0.0
18	1.4604669843182357E-4	0.0	0.0	6.610000547675119	0.0
19	1.4604669843182357E-4	0.0	0.0	6.810376617923581	0.0
20	1.4604669843182357E-4	0.0	0.0	7.083556967340307	0.0
21	1.4604669843182357E-4	0.0	0.0	7.337751245960896	0.0
22	1.4604669843182357E-4	0.0	0.0	7.63955674827026	0.0
23	1.4604669843182357E-4	0.0	0.0	7.930554794895668	0.0
24	1.4604669843182357E-4	0.0	0.0	8.153056939956551	0.0
25	1.4604669843182357E-4	0.0	0.0	8.344232068203809	0.0
26	1.4604669843182357E-4	0.0	0.0	8.508680650638041	0.0
27	1.4604669843182357E-4	0.0	0.0	8.73322744947697	0.0
28	1.4604669843182357E-4	0.0	0.0	8.930098398963068	0.0
29	1.4604669843182357E-4	0.0	0.0	9.13529401025978	0.0
30	1.4604669843182357E-4	0.0	0.0	9.434543695346587	0.0
31	1.4604669843182357E-4	0.0	0.0	9.629223944356207	0.0
32	1.4604669843182357E-4	0.0	0.0	9.830914434890555	0.0
33	1.4604669843182357E-4	0.0	0.0	10.024353286963507	0.0
34	1.4604669843182357E-4	0.0	0.0	10.225678660751775	0.0
35	1.4604669843182357E-4	0.0	0.0	10.44387242820892	0.0
36	1.4604669843182357E-4	0.0	0.0	10.642715008123847	0.0
37	1.4604669843182357E-4	0.0	0.0	10.838490607371707	0.0
38	1.4604669843182357E-4	0.0	0.0	11.061138799131022	0.0
39	1.4604669843182357E-4	0.0	0.0	11.28159629041386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCGA	45	3.8562575E-10	45.000004	13
ACGAACG	65	0.0	45.000004	1
TCACGGT	20	7.0337596E-4	45.0	38
TCGCAAA	20	7.0337596E-4	45.0	12
GTCGATT	20	7.0337596E-4	45.0	35
CGAGTTA	35	1.2121563E-7	45.0	34
CGAAATT	20	7.0337596E-4	45.0	42
GCGAACT	20	7.0337596E-4	45.0	45
TAGTTCG	20	7.0337596E-4	45.0	21
TACGCGA	35	1.2121563E-7	45.0	21
GTATCGG	85	0.0	45.0	2
ACGCTAC	40	6.8175723E-9	45.0	39
CCGAACG	20	7.0337596E-4	45.0	1
GACGATA	20	7.0337596E-4	45.0	42
CGGTATC	20	7.0337596E-4	45.0	23
TTCGCTA	20	7.0337596E-4	45.0	24
TAATGCG	35	1.2121563E-7	45.0	1
TACGTCC	20	7.0337596E-4	45.0	20
ACGATAG	30	2.1658088E-6	44.999996	1
TGCGACA	30	2.1658088E-6	44.999996	30
>>END_MODULE