Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553337_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1359179 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 12399 | 0.9122418754262683 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2607 | 0.19180696582275034 | No Hit |
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT | 2581 | 0.18989404633238152 | TruSeq Adapter, Index 13 (95% over 22bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2045 | 0.15045847530016282 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGT | 1781 | 0.13103498509026407 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCC | 1595 | 0.11735025335147173 | No Hit |
AATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT | 1572 | 0.11565805534076085 | No Hit |
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 1485 | 0.10925713243068058 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTAACG | 60 | 0.0 | 45.000004 | 1 |
ATCGCGC | 35 | 1.2121382E-7 | 45.000004 | 12 |
CCGATAG | 20 | 7.033743E-4 | 45.0 | 1 |
TCGTATC | 25 | 3.891183E-5 | 45.0 | 45 |
GCGATAT | 20 | 7.033743E-4 | 45.0 | 38 |
ACGTACG | 80 | 0.0 | 45.0 | 18 |
CGTTTAG | 20 | 7.033743E-4 | 45.0 | 1 |
TTCGACG | 20 | 7.033743E-4 | 45.0 | 33 |
TAATGCG | 40 | 6.8157533E-9 | 45.0 | 1 |
TCGCGGA | 20 | 7.033743E-4 | 45.0 | 18 |
ATCGACG | 25 | 3.891183E-5 | 45.0 | 1 |
TACGTAA | 20 | 7.033743E-4 | 45.0 | 25 |
CTTACGA | 20 | 7.033743E-4 | 45.0 | 40 |
CGTAAGG | 255 | 0.0 | 42.35294 | 2 |
GTCAACG | 70 | 0.0 | 41.785717 | 1 |
GCGTAAG | 130 | 0.0 | 41.53846 | 1 |
CGATACG | 55 | 6.184564E-11 | 40.90909 | 1 |
ACGCGAT | 105 | 0.0 | 40.714287 | 22 |
TAAGCGG | 205 | 0.0 | 40.609756 | 2 |
ATATCCG | 50 | 1.0822987E-9 | 40.5 | 1 |