FastQCFastQC Report
Sat 18 Jun 2016
SRR3553337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359179
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123990.9122418754262683No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26070.19180696582275034No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT25810.18989404633238152TruSeq Adapter, Index 13 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20450.15045847530016282No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGT17810.13103498509026407No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCC15950.11735025335147173No Hit
AATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT15720.11565805534076085No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14850.10925713243068058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG600.045.0000041
ATCGCGC351.2121382E-745.00000412
CCGATAG207.033743E-445.01
TCGTATC253.891183E-545.045
GCGATAT207.033743E-445.038
ACGTACG800.045.018
CGTTTAG207.033743E-445.01
TTCGACG207.033743E-445.033
TAATGCG406.8157533E-945.01
TCGCGGA207.033743E-445.018
ATCGACG253.891183E-545.01
TACGTAA207.033743E-445.025
CTTACGA207.033743E-445.040
CGTAAGG2550.042.352942
GTCAACG700.041.7857171
GCGTAAG1300.041.538461
CGATACG556.184564E-1140.909091
ACGCGAT1050.040.71428722
TAAGCGG2050.040.6097562
ATATCCG501.0822987E-940.51