##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553337_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1359179
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.679594078484143	31.0	31.0	34.0	30.0	34.0
2	31.8867993104661	33.0	31.0	34.0	30.0	34.0
3	32.05081449904685	33.0	31.0	34.0	30.0	34.0
4	35.77092568381354	37.0	35.0	37.0	35.0	37.0
5	35.534090064664035	37.0	35.0	37.0	33.0	37.0
6	32.73857380080181	37.0	35.0	37.0	26.0	37.0
7	34.26388650795811	37.0	35.0	37.0	28.0	37.0
8	35.391448072696825	37.0	35.0	37.0	32.0	37.0
9	37.22029107277261	39.0	37.0	39.0	34.0	39.0
10	36.73926098034181	39.0	37.0	39.0	32.0	39.0
11	36.76813134988107	39.0	37.0	39.0	32.0	39.0
12	36.77432258738547	39.0	37.0	39.0	32.0	39.0
13	36.77927704886553	39.0	37.0	39.0	33.0	39.0
14	37.94269408223641	40.0	37.0	41.0	33.0	41.0
15	37.98329432694295	40.0	37.0	41.0	33.0	41.0
16	37.91434020095955	40.0	37.0	41.0	33.0	41.0
17	37.91401868333752	40.0	37.0	41.0	33.0	41.0
18	37.828250730772034	40.0	37.0	41.0	33.0	41.0
19	37.84842025958317	40.0	37.0	41.0	33.0	41.0
20	37.7294822830547	39.0	36.0	41.0	33.0	41.0
21	37.69804345123048	39.0	36.0	41.0	33.0	41.0
22	37.62805634872228	39.0	36.0	41.0	33.0	41.0
23	37.497771080924586	39.0	36.0	41.0	32.0	41.0
24	37.39273855761456	39.0	36.0	41.0	32.0	41.0
25	37.367436518663105	39.0	36.0	41.0	32.0	41.0
26	37.31311401956622	39.0	36.0	41.0	32.0	41.0
27	37.24190338432245	39.0	36.0	41.0	32.0	41.0
28	37.14193126880271	39.0	36.0	41.0	31.0	41.0
29	36.96558437115347	39.0	35.0	40.0	31.0	41.0
30	37.00565267709404	39.0	35.0	40.0	31.0	41.0
31	36.669270934880544	39.0	35.0	40.0	30.0	41.0
32	36.77477065198918	39.0	35.0	40.0	31.0	41.0
33	36.747404867202924	39.0	35.0	40.0	31.0	41.0
34	36.73123849029451	39.0	35.0	40.0	31.0	41.0
35	36.694300750673754	39.0	35.0	40.0	31.0	41.0
36	36.6098917066847	39.0	35.0	40.0	30.0	41.0
37	36.51313623886184	39.0	35.0	40.0	30.0	41.0
38	36.44367371773696	39.0	35.0	40.0	30.0	41.0
39	36.38582408939514	39.0	35.0	40.0	30.0	41.0
40	36.272446822677516	38.0	35.0	40.0	30.0	41.0
41	36.24192251351735	38.0	35.0	40.0	30.0	41.0
42	36.11260400580056	38.0	35.0	40.0	30.0	41.0
43	36.068309619262806	38.0	35.0	40.0	30.0	41.0
44	35.91183869085676	38.0	35.0	40.0	29.0	41.0
45	35.75820256198779	38.0	34.0	40.0	29.0	41.0
46	35.47979920231257	38.0	34.0	40.0	28.0	41.0
47	35.46391755611292	38.0	34.0	40.0	28.0	41.0
48	35.406550572073286	38.0	34.0	40.0	28.0	41.0
49	35.291892385035375	37.0	34.0	40.0	27.0	41.0
50	35.18004251095698	37.0	34.0	40.0	27.0	41.0
51	33.997183593919566	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	6.0
10	11.0
11	15.0
12	21.0
13	32.0
14	47.0
15	81.0
16	162.0
17	285.0
18	491.0
19	998.0
20	1802.0
21	2802.0
22	3937.0
23	5123.0
24	6475.0
25	7877.0
26	9811.0
27	12186.0
28	16036.0
29	20532.0
30	27421.0
31	35363.0
32	46707.0
33	63641.0
34	91768.0
35	118355.0
36	143601.0
37	196961.0
38	268357.0
39	278238.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.06144885993677	31.528739040258863	22.164335970464524	26.245476129339846
2	23.47615729789822	30.583241795230798	21.464648879948854	24.475952026922133
3	20.64569861659134	31.729522012921034	20.973543587709933	26.651235782777693
4	20.67152302971132	31.947741982476185	20.107211780052516	27.273523207759983
5	18.230049169388284	35.764163513415085	17.472827346508442	28.53295997068819
6	18.763165116588766	42.4274506889821	17.463630618189363	21.345753576239773
7	86.40635265847986	6.3986421214571445	3.705692921977164	3.4893122980858298
8	87.69006878417045	5.549820884519257	3.169413300234921	3.5906970310753774
9	80.75065903755134	9.36638956311126	5.809683639903207	4.073267759434188
10	36.14056721005843	38.28943796218158	11.790573574194422	13.779421253565571
11	24.05886200419518	29.847871398837096	27.455544854651226	18.6377217423165
12	26.563535781526937	25.168134587129437	28.743307540802203	19.525022090541423
13	24.98979163156582	26.123711446395216	26.668819927323774	22.21767699471519
14	21.10641791846401	27.797589574294484	28.11248555193981	22.983506955301696
15	19.777674610923214	29.74030646441712	29.278336407493054	21.203682517166612
16	22.80501685208497	29.396054529977288	25.948679313026467	21.850249304911273
17	22.70959159904619	29.207264091043193	26.755122025870026	21.32802228404059
18	23.084670966811583	29.127730784539786	26.087660271384415	21.699937977264216
19	23.094824154875848	30.21986066588728	25.788214797315145	20.897100381921735
20	24.29569615186815	28.733080778911386	26.377614721828397	20.593608347392063
21	24.68225303657576	28.49933673195363	26.00945129375895	20.808958937711665
22	24.130817206563666	28.05244930947285	25.573526371434518	22.24320711252896
23	22.603277419677614	28.784950326631005	25.93646605781873	22.675306195872654
24	20.924175550093107	29.442332466878902	27.146240487823896	22.48725149520409
25	21.445004668259298	31.05278995628979	24.888186177096618	22.614019198354303
26	23.26617759691696	30.05682106624661	24.318724759579126	22.3582765772573
27	21.073088975035663	29.461240940302936	26.520347945340532	22.94532213932087
28	22.324579764696185	29.371039428949388	27.080024043926514	21.224356762427906
29	23.01624730811762	28.93246584886906	25.9037257050028	22.14756113801052
30	22.254978924777383	29.410107130848846	25.42932167139133	22.90559227298244
31	23.003813331430223	30.81845731871961	23.75706216767622	22.420667182173943
32	23.05126844955668	28.436946127036983	25.230010175260215	23.281775248146126
33	22.23290677681159	29.74825243768481	25.156730644013773	22.862110141489826
34	21.79205240810813	28.77818153458816	27.207306763862597	22.222459293441112
35	23.93040210303426	28.22056550314565	26.133349617673613	21.715682776146483
36	23.738595137211508	29.369641526244887	25.77453006557635	21.117233270967255
37	22.181037229091974	30.400042967114704	26.21972529004642	21.199194513746903
38	22.03359528068047	28.474248057099178	27.91788278070806	21.574273881512298
39	21.820010462198137	28.504045456853	27.00004929446379	22.675894786485078
40	22.26196843829989	28.723074738500227	26.695085783403066	22.31987103979682
41	21.355244599865067	28.330337652362196	28.556209299878827	21.758208447893914
42	21.433600725143634	28.854330445070147	26.25894013959898	23.45312869018724
43	21.770715998407862	29.41783238263687	25.716038873466996	23.095412745488268
44	22.594963577277166	26.97069333766928	26.110909600575056	24.3234334844785
45	22.629175406624146	28.575779937741828	26.517404992278426	22.2776396633556
46	20.873115314465572	29.459769463771877	28.268829933364188	21.398285288398363
47	21.532336800377287	28.30458681306877	27.69502766008009	22.46804872647385
48	20.896732512788972	27.4590028244992	29.066443787021427	22.5778208756904
49	21.526965911038943	28.66399495577845	27.14521045425216	22.663828678930443
50	21.16505625822647	26.95318276694975	28.39044746865571	23.491313506168062
51	21.770274555448545	26.725545347595865	27.894707025343973	23.60947307161161
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14470.0
1	10855.5
2	7241.0
3	4641.5
4	2042.0
5	1778.5
6	1515.0
7	1602.5
8	1690.0
9	2025.0
10	2360.0
11	2517.0
12	2674.0
13	2965.0
14	3256.0
15	3863.5
16	4471.0
17	4207.0
18	3943.0
19	4257.0
20	4571.0
21	4571.0
22	4571.0
23	6193.5
24	7816.0
25	9304.5
26	13014.0
27	15235.0
28	18447.5
29	21660.0
30	26226.0
31	30792.0
32	36413.5
33	42035.0
34	45871.0
35	49707.0
36	52402.5
37	55098.0
38	59351.5
39	63605.0
40	61923.5
41	60242.0
42	64017.5
43	67793.0
44	74262.0
45	80731.0
46	89018.0
47	97305.0
48	99963.5
49	102622.0
50	98925.5
51	95229.0
52	90583.5
53	85938.0
54	79921.5
55	73905.0
56	70264.0
57	66623.0
58	63107.5
59	59592.0
60	55494.5
61	51397.0
62	47243.0
63	43089.0
64	38904.0
65	34719.0
66	31871.5
67	29024.0
68	25072.0
69	21120.0
70	17256.5
71	13393.0
72	12143.0
73	10893.0
74	8761.0
75	5240.0
76	3851.0
77	3434.5
78	3018.0
79	2122.5
80	1227.0
81	932.5
82	638.0
83	439.5
84	241.0
85	228.0
86	215.0
87	173.0
88	131.0
89	80.0
90	29.0
91	23.0
92	17.0
93	13.0
94	9.0
95	8.0
96	7.0
97	5.0
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1359179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.012170082660177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.42752540799553	16.603235181949696
2	9.304125033287589	4.096079654061254
3	3.598980703505641	2.3766412610933445
4	1.9789636067357799	1.7424513399545045
5	1.2892534236887	1.4189632820943805
6	0.9078860688348125	1.199072553772177
7	0.661517487734149	1.0193004816860707
8	0.5388676814489454	0.9489317644882342
9	0.4223315852038373	0.8366791216307721
>10	3.8083201551871353	18.558460552931834
>50	1.1953822671903023	18.992622288043307
>100	0.8614330174128791	29.767823607599865
>500	0.001691738054620541	0.23028489870772134
>1k	0.003383476109241082	1.286005609956735
>5k	0.0	0.0
>10k+	3.383476109241082E-4	0.9234484020300896
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12399	0.9122418754262683	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2607	0.19180696582275034	No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	2581	0.18989404633238152	TruSeq Adapter, Index 13 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2045	0.15045847530016282	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGT	1781	0.13103498509026407	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCC	1595	0.11735025335147173	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT	1572	0.11565805534076085	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1485	0.10925713243068058	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22822600996631054	0.0
2	0.0	0.0	0.0	0.3098193836131959	0.0
3	0.0	0.0	0.0	0.4167221535941918	0.0
4	0.0	0.0	0.0	0.905031640424109	0.0
5	0.0	0.0	0.0	0.9855214066727046	0.0
6	0.0	0.0	0.0	1.4535245173740912	0.0
7	0.0	0.0	0.0	1.8300017878439852	0.0
8	0.0	0.0	0.0	2.1938979339733766	0.0
9	0.0	0.0	0.0	2.8111823387500836	0.0
10	0.0	0.0	0.0	3.6101940951118285	0.0
11	0.0	0.0	0.0	4.377274810749725	0.0
12	0.0	0.0	0.0	4.721011728403691	0.0
13	0.0	0.0	0.0	4.870366596305564	0.0
14	0.0	0.0	0.0	5.0331119006400185	0.0
15	0.0	0.0	0.0	5.1731228925697055	0.0
16	0.0	0.0	0.0	5.450054775713869	0.0
17	0.0	0.0	0.0	5.8360966436356065	0.0
18	0.0	0.0	0.0	6.35295277516795	0.0
19	0.0	0.0	0.0	6.5474083987465965	0.0
20	0.0	0.0	0.0	6.80852190918194	0.0
21	0.0	0.0	0.0	7.071621912934205	0.0
22	0.0	0.0	0.0	7.363783578174766	0.0
23	0.0	0.0	0.0	7.651457239995615	0.0
24	0.0	0.0	0.0	7.8629084175079225	0.0
25	0.0	0.0	0.0	8.042502128122933	0.0
26	0.0	0.0	0.0	8.203849529752887	0.0
27	0.0	0.0	0.0	8.421922351654933	0.0
28	0.0	0.0	0.0	8.615715810794605	0.0
29	0.0	0.0	0.0	8.81046572967946	0.0
30	0.0	0.0	0.0	9.098654408286178	0.0
31	0.0	0.0	0.0	9.294581508395877	0.0
32	0.0	0.0	0.0	9.495438054884604	0.0
33	0.0	0.0	0.0	9.685626396523196	0.0
34	0.0	0.0	0.0	9.872503915966918	0.0
35	0.0	0.0	0.0	10.079761385365725	0.0
36	7.357382655264685E-5	0.0	0.0	10.282310129865161	0.0
37	7.357382655264685E-5	0.0	0.0	10.485815334109782	0.0
38	7.357382655264685E-5	0.0	0.0	10.70535963254288	0.0
39	7.357382655264685E-5	0.0	0.0	10.92299101148561	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	60	0.0	45.000004	1
ATCGCGC	35	1.2121382E-7	45.000004	12
CCGATAG	20	7.033743E-4	45.0	1
TCGTATC	25	3.891183E-5	45.0	45
GCGATAT	20	7.033743E-4	45.0	38
ACGTACG	80	0.0	45.0	18
CGTTTAG	20	7.033743E-4	45.0	1
TTCGACG	20	7.033743E-4	45.0	33
TAATGCG	40	6.8157533E-9	45.0	1
TCGCGGA	20	7.033743E-4	45.0	18
ATCGACG	25	3.891183E-5	45.0	1
TACGTAA	20	7.033743E-4	45.0	25
CTTACGA	20	7.033743E-4	45.0	40
CGTAAGG	255	0.0	42.35294	2
GTCAACG	70	0.0	41.785717	1
GCGTAAG	130	0.0	41.53846	1
CGATACG	55	6.184564E-11	40.90909	1
ACGCGAT	105	0.0	40.714287	22
TAAGCGG	205	0.0	40.609756	2
ATATCCG	50	1.0822987E-9	40.5	1
>>END_MODULE