SRR3553335_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256207
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20095	7.843267358034714	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGT	5988	2.3371726767808845	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	5844	2.2809681234314443	No Hit
AATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT	4813	1.8785591338253835	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4243	1.6560827768171829	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3802	1.4839563321845226	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	2594	1.0124625790864419	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1653	0.6451814353237811	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTT	1538	0.6002958545238811	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	1288	0.5027185049588809	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTAT	988	0.38562568548088066	No Hit
AACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG	870	0.3395691764862006	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGCTAGTC	774	0.3020994742532405	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	463	0.18071325139438033	No Hit
CCTACCGGGATTTCTGTAGTACCGGGTTTTTTGTTTTTTTGTTTTTTTGCA	399	0.15573344990574028	No Hit
AAACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT	382	0.14909819013532027	No Hit
AATGAAACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGT	373	0.14558540555098026	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC	363	0.14168231156838026	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	361	0.14090169277186027	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	326	0.12724086383276023	No Hit
AATGAAACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	319	0.12450869804494023	No Hit
ATCGTAGGGGTTGTTTTGTGGGTGAGTGGTGTTTGTTTGTTTGTTTGTTTG	313	0.12216684165538022	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	307	0.11982498526582022	No Hit
GAGAATGGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGA	262	0.10226106234412019	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAGC	25	3.8822935E-5	45.000004	30
AACGTCT	25	3.8822935E-5	45.000004	33
CAAACTC	25	3.8822935E-5	45.000004	19
ACTGCGG	25	3.8822935E-5	45.000004	2
ATTAGAA	25	3.8822935E-5	45.000004	41
TGCCATA	25	3.8822935E-5	45.000004	38
GTTTCCG	25	3.8822935E-5	45.000004	34
CTATCTT	25	3.8822935E-5	45.000004	16
AACCATT	50	2.1827873E-11	45.000004	14
CTATAGT	25	3.8822935E-5	45.000004	28
TTATGGG	50	2.1827873E-11	45.000004	3
GCAGTAG	25	3.8822935E-5	45.000004	1
CCAGCGG	25	3.8822935E-5	45.000004	2
CGTCTCA	25	3.8822935E-5	45.000004	28
CACGAGG	25	3.8822935E-5	45.000004	2
CAACAAT	25	3.8822935E-5	45.000004	26
ATCGTAG	55	1.8189894E-12	45.000004	1
ATATCAC	25	3.8822935E-5	45.000004	30
GGGACGT	25	3.8822935E-5	45.000004	7
GAGGTTA	25	3.8822935E-5	45.000004	31

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content