##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256207
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28966811991866	31.0	31.0	34.0	30.0	34.0
2	31.480248392901053	31.0	31.0	34.0	30.0	34.0
3	31.637453309238232	31.0	31.0	34.0	30.0	34.0
4	35.37028262303528	37.0	35.0	37.0	33.0	37.0
5	35.11156994149262	37.0	35.0	37.0	32.0	37.0
6	32.58391456907891	35.0	35.0	37.0	26.0	37.0
7	33.82913425472372	35.0	35.0	37.0	26.0	37.0
8	34.77018192321053	37.0	35.0	37.0	32.0	37.0
9	36.559590487379346	38.0	35.0	39.0	32.0	39.0
10	36.260512007868634	37.0	35.0	39.0	32.0	39.0
11	36.32859367620713	38.0	35.0	39.0	32.0	39.0
12	36.21807366699583	38.0	35.0	39.0	32.0	39.0
13	36.290819532643525	38.0	35.0	39.0	32.0	39.0
14	37.24898617133802	39.0	36.0	40.0	32.0	41.0
15	37.44612754530516	39.0	36.0	40.0	32.0	41.0
16	37.23163301549138	39.0	36.0	40.0	32.0	41.0
17	37.13425862681348	39.0	36.0	40.0	32.0	41.0
18	36.664119247327356	38.0	36.0	40.0	31.0	41.0
19	36.31360579531395	38.0	34.0	40.0	30.0	41.0
20	36.35484978942808	38.0	35.0	40.0	30.0	41.0
21	36.31491333179812	38.0	35.0	40.0	30.0	41.0
22	36.158816894152	38.0	34.0	40.0	30.0	41.0
23	35.885510544208394	38.0	34.0	40.0	30.0	41.0
24	35.87259910931395	38.0	34.0	40.0	30.0	41.0
25	35.8493483784596	37.0	34.0	40.0	30.0	41.0
26	35.66842435999016	37.0	34.0	40.0	29.0	41.0
27	35.63399126487567	37.0	34.0	40.0	29.0	41.0
28	35.5096230782141	37.0	34.0	40.0	29.0	41.0
29	35.27338441182325	37.0	34.0	40.0	27.0	41.0
30	35.33883149172349	37.0	34.0	40.0	27.0	41.0
31	34.70410644517909	37.0	33.0	40.0	25.0	41.0
32	34.76682135929151	37.0	33.0	40.0	25.0	41.0
33	34.663217632617375	37.0	33.0	40.0	25.0	41.0
34	34.454043019901874	37.0	33.0	40.0	24.0	41.0
35	34.58443368057859	37.0	33.0	40.0	25.0	41.0
36	34.273040939552786	36.0	33.0	40.0	23.0	41.0
37	34.33803526055104	37.0	33.0	40.0	24.0	41.0
38	34.23222238268275	37.0	33.0	40.0	23.0	41.0
39	34.00505840980145	36.0	33.0	40.0	22.0	41.0
40	33.793881509872875	36.0	33.0	40.0	22.0	41.0
41	33.709555164378806	36.0	33.0	40.0	21.0	41.0
42	33.67363499045693	36.0	33.0	40.0	21.0	41.0
43	33.63392100918398	36.0	33.0	40.0	21.0	41.0
44	33.5108564559126	36.0	33.0	40.0	21.0	41.0
45	33.30420324190986	35.0	33.0	40.0	20.0	41.0
46	32.83702240766255	35.0	32.0	39.0	20.0	41.0
47	32.8908929108104	35.0	32.0	39.0	20.0	41.0
48	32.91702412502391	35.0	32.0	39.0	20.0	41.0
49	32.782129293891266	35.0	31.0	39.0	19.0	41.0
50	32.61169288895307	35.0	31.0	39.0	18.0	40.0
51	31.399415316521406	35.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	2.0
11	2.0
12	4.0
13	3.0
14	8.0
15	26.0
16	54.0
17	120.0
18	274.0
19	636.0
20	1082.0
21	1707.0
22	2205.0
23	2733.0
24	3108.0
25	3425.0
26	3810.0
27	4243.0
28	4909.0
29	6158.0
30	7637.0
31	9988.0
32	12821.0
33	16120.0
34	22086.0
35	34314.0
36	26541.0
37	30045.0
38	34389.0
39	27750.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.054233490888228	47.4272756013692	15.085067933350768	22.4334229743918
2	14.210774881248367	50.57394997014133	16.23726127701429	18.978013871596016
3	14.903183753761606	39.69993013461771	27.075762957296252	18.321123154324432
4	24.190205575960064	38.58208401800107	15.084677623952508	22.14303278208636
5	11.834571264641482	51.149656332574835	16.44802835207469	20.567744050708995
6	15.23806921746869	46.00967186688889	23.070798221750383	15.681460693892049
7	58.47029940633941	29.243151045834033	7.2176013926239335	5.068948155202629
8	60.32465935747267	20.826128872357117	4.9678580210532886	13.881353749116926
9	59.81413466454859	23.888106101706825	9.917371500388358	6.3803877333562315
10	26.108966577806225	48.629428548010004	13.327504712985986	11.934100161197781
11	15.98199893055225	36.7413848958069	29.270863013110493	18.005753160530354
12	18.322294082519214	32.86249009589902	34.87258349693803	13.942632324643744
13	19.315631501090916	40.15464058359061	23.328792734000242	17.20093518131823
14	11.15582322106734	40.85329440647602	31.003446431986635	16.98743594047001
15	9.958353987205658	41.819700476567775	25.571900845800467	22.650044690426103
16	13.210802202906244	42.573387924607836	26.98599179569645	17.22981807678947
17	10.630857080407639	42.09291705534978	25.861120109911127	21.41510575433146
18	12.722134836284724	42.33764104805879	23.383045740358384	21.5571783752981
19	13.832955383732685	44.42618663814806	27.94927539060213	13.791582587517127
20	14.465256608913887	46.575620494365886	23.205064654751823	15.754058241968409
21	13.226414578836643	42.43014437544642	25.112116374650185	19.231324671066755
22	13.687369978181705	45.0093088791485	23.62074416389872	17.682576978771074
23	15.106534950255067	41.624545777437774	20.43152607071626	22.8373932015909
24	10.75185299386824	48.853466142611246	21.639923967729217	18.754756895791296
25	13.967221816734124	41.93991577123185	23.871713106979904	20.221149305054116
26	16.71382905228975	45.1088377757048	19.181755377487736	18.995577794517715
27	11.607020885455901	41.844680278056416	26.518401136580966	20.029897699906716
28	20.711377909268677	39.53834204373807	22.21914311474706	17.53113693224619
29	13.918433141951624	44.74663065411952	24.335790981511042	16.99914522241781
30	17.48469011385325	39.40095313555055	23.66562974469862	19.448727005897577
31	22.2050919764097	43.22286276331248	18.36249595053999	16.209549309737827
32	16.51711311556671	38.17538162501415	21.54624971214682	23.761255547272324
33	21.4303278208636	38.98566393580191	23.604351169171803	15.97965707416269
34	15.745081125808428	41.15929697471186	23.514189698173745	19.581432201305976
35	21.155159695090298	37.74057695535251	25.39899378237129	15.705269567185908
36	18.791445979227735	37.638706202406645	29.29233003001479	14.277517788350828
37	17.97296717107651	38.46733305491263	23.10241328300944	20.457286491001415
38	21.54703033094334	37.738235098962946	25.315857880541902	15.398876689551807
39	16.52335806593887	39.69641735003337	23.488038968490322	20.292185615537438
40	20.869843524962235	39.60820742602661	23.28859086597946	16.23335818303169
41	16.72670926243233	38.69878652808081	27.79237101250161	16.78213319698525
42	23.814727934833943	37.23083288122495	20.528713110882997	18.42572607305811
43	17.615053452872093	36.98493796032115	26.96218292240259	18.437825664404173
44	17.857045279793294	35.2484514474624	24.528994133649746	22.36550913909456
45	23.891618886291162	37.88343019511567	22.397514509751883	15.827436408841288
46	14.953143356738888	36.50993142263872	31.443715433223918	17.093209787398468
47	18.867556311888436	38.59652546573669	23.20779682053964	19.328121401835237
48	17.436291748469014	34.939326404040486	29.572572177965473	18.05180966952503
49	19.724285441069135	33.86948834340982	25.244431260660328	21.161794954860717
50	17.34456903987791	34.453391203206785	22.96463406542366	25.237405691491645
51	15.531972194358467	33.844898851319435	30.480041528919976	20.14308742540212
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22281.0
1	16543.5
2	10806.0
3	6693.0
4	2580.0
5	1962.5
6	1345.0
7	1423.5
8	1502.0
9	1670.5
10	1839.0
11	2098.5
12	2358.0
13	2717.0
14	3076.0
15	2964.0
16	2852.0
17	2808.0
18	2764.0
19	3043.5
20	3323.0
21	3117.0
22	2911.0
23	2780.5
24	2650.0
25	2756.0
26	2487.5
27	2113.0
28	2730.5
29	3348.0
30	3483.5
31	3619.0
32	4295.5
33	4972.0
34	5949.5
35	6927.0
36	7140.5
37	7354.0
38	7024.0
39	6694.0
40	7380.0
41	8066.0
42	9481.0
43	10896.0
44	20388.5
45	29881.0
46	28135.0
47	26389.0
48	25354.0
49	24319.0
50	20869.0
51	17419.0
52	15103.5
53	12788.0
54	10438.5
55	8089.0
56	6708.5
57	5328.0
58	4783.5
59	4239.0
60	3650.0
61	3061.0
62	2759.0
63	2457.0
64	2150.0
65	1843.0
66	1501.5
67	1160.0
68	955.0
69	750.0
70	640.0
71	530.0
72	452.5
73	375.0
74	280.0
75	164.5
76	144.0
77	111.5
78	79.0
79	48.0
80	17.0
81	12.5
82	8.0
83	6.5
84	5.0
85	3.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	256207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.89410515715808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.73587099683188	26.228401253673788
2	8.513592083249286	5.60093986503101
3	3.261862667157113	3.2188816074502262
4	1.7561137676946261	2.310631637699204
5	1.1426605123580573	1.8793397526219033
6	0.7807586886101783	1.5409415043304828
7	0.6680351697378881	1.5382093385426627
8	0.5007297364642784	1.3176845285257623
9	0.43546875185400524	1.2891919424527822
>10	2.700618199508763	17.48703197024281
>50	0.32393179633826547	7.431881252268674
>100	0.1649323065605088	8.888906235973256
>500	0.003559690069651269	1.0272943362203217
>1k	0.00830594349585296	7.779256616720074
>5k	0.002373126713100846	4.618140800212328
>10k+	0.001186563356550423	7.843267358034714
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20095	7.843267358034714	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGT	5988	2.3371726767808845	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	5844	2.2809681234314443	No Hit
AATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT	4813	1.8785591338253835	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4243	1.6560827768171829	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3802	1.4839563321845226	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	2594	1.0124625790864419	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1653	0.6451814353237811	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTT	1538	0.6002958545238811	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	1288	0.5027185049588809	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTAT	988	0.38562568548088066	No Hit
AACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG	870	0.3395691764862006	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGCTAGTC	774	0.3020994742532405	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	463	0.18071325139438033	No Hit
CCTACCGGGATTTCTGTAGTACCGGGTTTTTTGTTTTTTTGTTTTTTTGCA	399	0.15573344990574028	No Hit
AAACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT	382	0.14909819013532027	No Hit
AATGAAACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGT	373	0.14558540555098026	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC	363	0.14168231156838026	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	361	0.14090169277186027	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	326	0.12724086383276023	No Hit
AATGAAACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	319	0.12450869804494023	No Hit
ATCGTAGGGGTTGTTTTGTGGGTGAGTGGTGTTTGTTTGTTTGTTTGTTTG	313	0.12216684165538022	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	307	0.11982498526582022	No Hit
GAGAATGGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGA	262	0.10226106234412019	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.202543256039062	0.0
2	0.0	0.0	0.0	1.4656117904663026	0.0
3	0.0	0.0	0.0	2.1513854032091237	0.0
4	0.0	0.0	0.0	5.48501797374779	0.0
5	0.0	0.0	0.0	5.86517932765303	0.0
6	0.0	0.0	0.0	7.630548735983013	0.0
7	0.0	0.0	0.0	9.071571034358936	0.0
8	0.0	0.0	0.0	9.957183059010879	0.0
9	0.0	0.0	0.0	13.793534134508425	0.0
10	0.0	0.0	0.0	15.747813291596248	0.0
11	0.0	0.0	0.0	19.942468394696476	0.0
12	0.0	0.0	0.0	20.9482957140125	0.0
13	0.0	0.0	0.0	21.49785134676258	0.0
14	0.0	0.0	0.0	22.26910271772434	0.0
15	0.0	0.0	0.0	22.69453996182774	0.0
16	0.0	0.0	0.0	23.29756798213944	0.0
17	0.0	0.0	0.0	24.050865120781243	0.0
18	0.0	0.0	0.0	25.615225189007326	0.0
19	0.0	0.0	0.0	26.024269438383808	0.0
20	0.0	0.0	0.0	26.522304230563567	0.0
21	0.0	0.0	0.0	26.93876435850699	0.0
22	0.0	0.0	0.0	27.37083686238081	0.0
23	0.0	0.0	0.0	27.78339389634163	0.0
24	0.0	0.0	0.0	28.049194596556692	0.0
25	0.0	0.0	0.0	28.28220930731791	0.0
26	0.0	0.0	0.0	28.49414731057309	0.0
27	0.0	0.0	0.0	28.804833591588054	0.0
28	0.0	0.0	0.0	29.074927695183973	0.0
29	0.0	0.0	0.0	29.321993544282552	0.0
30	0.0	0.0	0.0	29.734160268845113	0.0
31	0.0	0.0	0.0	29.973810239376753	0.0
32	0.0	0.0	0.0	30.217753613289254	0.0
33	0.0	0.0	0.0	30.439059042102674	0.0
34	0.0	0.0	0.0	30.673244681058677	0.0
35	0.0	0.0	0.0	30.932800430901576	0.0
36	0.0	0.0	0.0	31.153325240918477	0.0
37	0.0	0.0	0.0	31.371508194545818	0.0
38	0.0	0.0	0.0	31.61154847447572	0.0
39	0.0	0.0	0.0	31.865639892742976	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAGC	25	3.8822935E-5	45.000004	30
AACGTCT	25	3.8822935E-5	45.000004	33
CAAACTC	25	3.8822935E-5	45.000004	19
ACTGCGG	25	3.8822935E-5	45.000004	2
ATTAGAA	25	3.8822935E-5	45.000004	41
TGCCATA	25	3.8822935E-5	45.000004	38
GTTTCCG	25	3.8822935E-5	45.000004	34
CTATCTT	25	3.8822935E-5	45.000004	16
AACCATT	50	2.1827873E-11	45.000004	14
CTATAGT	25	3.8822935E-5	45.000004	28
TTATGGG	50	2.1827873E-11	45.000004	3
GCAGTAG	25	3.8822935E-5	45.000004	1
CCAGCGG	25	3.8822935E-5	45.000004	2
CGTCTCA	25	3.8822935E-5	45.000004	28
CACGAGG	25	3.8822935E-5	45.000004	2
CAACAAT	25	3.8822935E-5	45.000004	26
ATCGTAG	55	1.8189894E-12	45.000004	1
ATATCAC	25	3.8822935E-5	45.000004	30
GGGACGT	25	3.8822935E-5	45.000004	7
GAGGTTA	25	3.8822935E-5	45.000004	31
>>END_MODULE