SRR3553332_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284488
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17431	6.12714771800568	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4008	1.408846770338292	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3176	1.1163915525435169	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGT	2504	0.8801777227861983	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC	2317	0.8144456005174208	No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	2019	0.7096960152976575	TruSeq Adapter, Index 1 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	2008	0.7058294198700824	RNA PCR Primer, Index 33 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1962	0.6896600208093135	No Hit
AATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	596	0.20949917043952643	No Hit
TGAGTGGAGGGATAGTTTAAAGTTTTCACCATGTTATATTTTCTTTTGAGA	544	0.191220719327353	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	502	0.1764573549675206	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	479	0.1683726554371362	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	476	0.16731812941143387	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	471	0.16556058603526333	RNA PCR Primer, Index 33 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	455	0.15993644723151768	No Hit
AACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	435	0.15290627372683557	Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTAT	427	0.15009420432496273	No Hit
AATGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	321	0.11283428475014765	RNA PCR Primer, Index 33 (95% over 21bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	306	0.10756165462163607	No Hit
AATCACGGGAACTCTAATTACTTGTTTTTCTTTTGGCTTTTGTGAATTTGG	302	0.10615561992069963	No Hit
AGATTAGGGGAAAGGAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGA	296	0.10404656786929502	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	288	0.10123449846742218	No Hit
GCTGAAGGGGGTTTGTAATAACTGTGGCACCTCAATATTTTTTATTTGCTT	286	0.10053148111695398	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCAG	30	2.1597225E-6	45.000004	14
AAATCCG	30	2.1597225E-6	45.000004	1
GCGACAG	30	2.1597225E-6	45.000004	1
AGTCAGC	30	2.1597225E-6	45.000004	11
TAGCGAG	30	2.1597225E-6	45.000004	1
GCTGCGC	30	2.1597225E-6	45.000004	9
TTCAATC	30	2.1597225E-6	45.000004	37
CTGCGCC	30	2.1597225E-6	45.000004	10
GTCAGAC	30	2.1597225E-6	45.000004	9
TGACCTT	30	2.1597225E-6	45.000004	34
TGCGCCG	30	2.1597225E-6	45.000004	11
GTTGTCA	30	2.1597225E-6	45.000004	10
TTGACAG	30	2.1597225E-6	45.000004	1
TCATGTA	30	2.1597225E-6	45.000004	34
CTACCGC	30	2.1597225E-6	45.000004	2
CGCCGTG	30	2.1597225E-6	45.000004	13
TACAGGT	30	2.1597225E-6	45.000004	39
CACCGAG	55	1.8189894E-12	45.000004	1
TCAAGAC	30	2.1597225E-6	45.000004	34
GTGCGGA	30	2.1597225E-6	45.000004	29

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content