##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284488
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.508235848260735	31.0	31.0	34.0	30.0	34.0
2	31.6903911588538	31.0	31.0	34.0	30.0	34.0
3	31.880395658164844	31.0	31.0	34.0	30.0	34.0
4	35.61131928235989	37.0	35.0	37.0	33.0	37.0
5	35.314139787969964	37.0	35.0	37.0	33.0	37.0
6	33.924720902111865	37.0	35.0	37.0	31.0	37.0
7	34.64553162172042	37.0	35.0	37.0	31.0	37.0
8	35.33194370237057	37.0	35.0	37.0	32.0	37.0
9	36.99003121397036	39.0	37.0	39.0	33.0	39.0
10	36.575700908298415	38.0	35.0	39.0	32.0	39.0
11	36.61912629003684	38.0	35.0	39.0	32.0	39.0
12	36.4858904417761	38.0	35.0	39.0	32.0	39.0
13	36.469643710806785	38.0	35.0	39.0	32.0	39.0
14	37.53955878631085	39.0	36.0	40.0	32.0	41.0
15	37.639555974241446	39.0	36.0	41.0	32.0	41.0
16	37.51291091364135	39.0	36.0	41.0	32.0	41.0
17	37.3841673462501	39.0	36.0	40.0	32.0	41.0
18	37.03456736312252	38.0	36.0	40.0	32.0	41.0
19	36.74580298641771	38.0	35.0	40.0	31.0	41.0
20	36.69381133826383	38.0	35.0	40.0	31.0	41.0
21	36.66639014650882	38.0	35.0	40.0	31.0	41.0
22	36.59900944855319	38.0	35.0	40.0	31.0	41.0
23	36.34599350411968	38.0	35.0	40.0	31.0	41.0
24	36.2038223053345	38.0	35.0	40.0	30.0	41.0
25	36.226849638649085	38.0	35.0	40.0	30.0	41.0
26	36.20199797531003	38.0	35.0	40.0	31.0	41.0
27	36.168007086414896	38.0	35.0	40.0	31.0	41.0
28	35.98664970051461	38.0	35.0	40.0	30.0	41.0
29	35.734417620426875	38.0	34.0	40.0	29.0	41.0
30	35.7132040718765	38.0	34.0	40.0	29.0	41.0
31	35.49325806360901	38.0	34.0	40.0	27.0	41.0
32	35.41518798683951	38.0	34.0	40.0	27.0	41.0
33	35.203945333370825	38.0	34.0	40.0	26.0	41.0
34	35.12598773937741	38.0	34.0	40.0	26.0	41.0
35	34.88536599083265	37.0	34.0	40.0	25.0	41.0
36	34.738807963780545	37.0	33.0	40.0	25.0	41.0
37	34.69942844689407	37.0	33.0	40.0	24.0	41.0
38	34.47589353505244	37.0	33.0	40.0	24.0	41.0
39	34.27956539467394	37.0	33.0	40.0	23.0	41.0
40	34.14302184977925	37.0	33.0	40.0	22.0	41.0
41	34.03721422344704	37.0	33.0	40.0	22.0	41.0
42	34.01176499536009	37.0	33.0	40.0	22.0	41.0
43	33.86226835578302	37.0	33.0	40.0	21.0	41.0
44	33.79928151626782	36.0	33.0	40.0	21.0	41.0
45	33.580720452180756	36.0	33.0	40.0	20.0	41.0
46	33.330182643907655	36.0	33.0	40.0	20.0	41.0
47	33.25875608110008	36.0	32.0	40.0	20.0	41.0
48	33.091367649953604	35.0	32.0	40.0	20.0	41.0
49	32.88149939540508	35.0	31.0	39.0	18.0	41.0
50	32.72957031579539	35.0	31.0	39.0	17.0	41.0
51	31.495739714856164	35.0	29.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	4.0
12	4.0
13	5.0
14	9.0
15	26.0
16	41.0
17	130.0
18	261.0
19	536.0
20	946.0
21	1584.0
22	2150.0
23	2757.0
24	3264.0
25	3728.0
26	4038.0
27	4376.0
28	4928.0
29	6328.0
30	7727.0
31	9798.0
32	12137.0
33	15845.0
34	22767.0
35	35052.0
36	28044.0
37	34414.0
38	43963.0
39	39614.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.178271139731727	41.13987233204915	18.2120159725542	22.469840555664913
2	17.925536402238407	43.3171170664492	17.52165293439442	21.235693596917972
3	16.091364134866847	41.576446106689914	21.319704170298923	21.012485588144315
4	19.289390062146733	40.09483704057816	17.626402519614185	22.98937037766092
5	15.030510953010321	45.84762801945952	15.875186299597873	23.246674727932284
6	16.099448834397233	46.89582688900762	18.231348949692077	18.773375326903068
7	68.96635358960658	21.46839233992295	5.418154720058491	4.147099350411968
8	70.0166615112061	18.339965130339415	4.065549337757656	7.5778240206968315
9	65.51032029470487	20.363600573662158	9.062948173560924	5.063130958072045
10	29.442366637608615	40.46181209752257	14.018868985686566	16.07695227918225
11	20.905978459548383	40.592924833384885	23.413641348668484	15.087455358398245
12	22.218160344197294	33.98737380838559	28.814923652315738	14.979542195101375
13	21.497216051292146	36.66586991366947	24.394350552571638	17.442563482466745
14	14.610106577430331	40.78766063946458	27.480596721127075	17.12163606197801
15	13.990396782992603	40.05722561232811	26.391974353927054	19.560403250752227
16	15.780630465959899	40.45407890666742	25.48473046314783	18.280560164224855
17	15.940215404516184	38.64697280728888	24.525814797109195	20.88699699108574
18	17.74345490846714	37.999845336182894	24.005933466437952	20.25076628891201
19	17.632026658417928	41.92830629059925	25.25484378954473	15.184823261438094
20	17.137453952363543	40.50575068192683	23.133488934506904	19.223306431202722
21	17.150108264671974	39.33592981074773	24.305770366412645	19.208191558167655
22	16.96943280560164	39.69095357273418	23.44914372486713	19.890469896797054
23	16.71001940327887	39.802733331458626	21.864191108236554	21.623056157025957
24	14.593937178369561	41.86819830713422	22.91168696043418	20.626177554062032
25	15.193962486994177	40.43755799893141	23.50995472568263	20.858524788391776
26	18.63804448693794	39.35034166643233	22.377393774078342	19.63422007255139
27	15.791878743567391	39.64631197097944	23.327170214560898	21.23463907089227
28	18.03450409156098	38.622718708697725	24.741289615027696	18.601487584713592
29	18.175810579005088	41.7834847164028	21.90531762323894	18.13538708135317
30	20.543221506706786	39.42345547088102	20.39383031973229	19.639492702679902
31	18.550518826804645	41.35253508056579	19.478150220747448	20.618795871882117
32	20.55903939709232	38.30882146171368	19.81138044486938	21.320758696324628
33	18.057703664126432	39.03785045414921	23.49976097410084	19.40468490762352
34	18.0573521554512	39.27968842271027	22.283892466466074	20.37906695537246
35	19.312238126036952	38.5327324878378	21.67050982818256	20.48451955794269
36	18.703425100531483	39.439976378617025	23.9363347487416	17.920263772109895
37	20.143907651640845	38.58581029779815	20.84551896740812	20.424763083152893
38	20.54884564551053	36.96148870954135	22.035727341750793	20.453938303197322
39	19.70627935097438	37.468012710553694	22.503233879812154	20.32247405865977
40	19.655310593065437	38.410407468856334	21.252917522004445	20.681364416073787
41	16.325820421247997	36.02647563341863	25.42391946233233	22.22378448300104
42	20.527755124996485	34.984603920024746	23.64528556564776	20.842355389331008
43	18.884452068277042	35.206757402772695	25.386308034082283	20.522482494867976
44	21.01635218357189	31.46108095947808	25.05413233598605	22.468434520963978
45	19.896445544276034	34.477728410337164	25.247110598689577	20.378715446697225
46	18.15366553246534	33.41511768510447	28.97099350411968	19.460223278310508
47	19.07989089170721	34.826073507494165	25.62920052866905	20.46483507212958
48	18.88585810297798	32.836534405669134	28.191698771125672	20.085908720227216
49	19.3797277916819	31.6828829335508	26.259104074688562	22.678285200078736
50	19.233851691459744	31.88253986108377	24.636188521132702	24.247419926323783
51	17.617263294058098	31.24033351143106	29.717949438992154	21.424453755518684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20033.0
1	15007.5
2	9982.0
3	6165.0
4	2348.0
5	1968.0
6	1588.0
7	1628.0
8	1668.0
9	1810.0
10	1952.0
11	2028.0
12	2104.0
13	2360.0
14	2616.0
15	2328.5
16	2041.0
17	1996.0
18	1951.0
19	1955.5
20	1960.0
21	1815.0
22	1670.0
23	1949.0
24	2228.0
25	2032.0
26	2079.5
27	2323.0
28	3181.0
29	4039.0
30	4786.0
31	5533.0
32	5579.0
33	5625.0
34	5626.0
35	5627.0
36	6960.5
37	8294.0
38	8355.5
39	8417.0
40	9354.5
41	10292.0
42	11527.0
43	12762.0
44	17869.0
45	22976.0
46	24154.5
47	25333.0
48	25290.0
49	25247.0
50	23776.5
51	22306.0
52	19500.5
53	16695.0
54	14757.0
55	12819.0
56	11596.0
57	10373.0
58	9219.0
59	8065.0
60	7585.0
61	7105.0
62	6299.5
63	5494.0
64	4774.0
65	4054.0
66	3182.0
67	2310.0
68	2122.5
69	1935.0
70	1633.5
71	1332.0
72	974.5
73	617.0
74	412.5
75	356.0
76	504.0
77	292.5
78	81.0
79	57.0
80	33.0
81	31.0
82	29.0
83	21.0
84	13.0
85	35.0
86	57.0
87	33.0
88	9.0
89	5.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	284488.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.680422372824165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.81152151963342	25.601431343325554
2	8.402587459917672	5.323950395095751
3	3.021292176595249	2.871474367987402
4	1.5555826777769148	1.9712606507128596
5	0.9553186059671352	1.513244846882821
6	0.6757131603182176	1.2844126993054188
7	0.4671186215007711	1.0358960659149068
8	0.33730180744948796	0.8548690981693428
9	0.2518668101678742	0.7181322235032761
>10	2.6229653710874654	18.40956384803577
>50	0.602483162648263	13.55593206040325
>100	0.28404362732588456	13.830460335761085
>500	0.0033286362577252104	0.5771772447344
>1k	0.007766817934692157	6.325047102162482
>5k	0.0	0.0
>10k+	0.0011095454192417366	6.12714771800568
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17431	6.12714771800568	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4008	1.408846770338292	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3176	1.1163915525435169	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGT	2504	0.8801777227861983	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC	2317	0.8144456005174208	No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	2019	0.7096960152976575	TruSeq Adapter, Index 1 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	2008	0.7058294198700824	RNA PCR Primer, Index 33 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1962	0.6896600208093135	No Hit
AATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	596	0.20949917043952643	No Hit
TGAGTGGAGGGATAGTTTAAAGTTTTCACCATGTTATATTTTCTTTTGAGA	544	0.191220719327353	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	502	0.1764573549675206	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	479	0.1683726554371362	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	476	0.16731812941143387	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	471	0.16556058603526333	RNA PCR Primer, Index 33 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	455	0.15993644723151768	No Hit
AACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	435	0.15290627372683557	Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTAT	427	0.15009420432496273	No Hit
AATGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	321	0.11283428475014765	RNA PCR Primer, Index 33 (95% over 21bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	306	0.10756165462163607	No Hit
AATCACGGGAACTCTAATTACTTGTTTTTCTTTTGGCTTTTGTGAATTTGG	302	0.10615561992069963	No Hit
AGATTAGGGGAAAGGAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGA	296	0.10404656786929502	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	288	0.10123449846742218	No Hit
GCTGAAGGGGGTTTGTAATAACTGTGGCACCTCAATATTTTTTATTTGCTT	286	0.10053148111695398	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.515086752341048E-4	0.0	0.0	0.8974016478726695	0.0
2	3.515086752341048E-4	0.0	0.0	1.1385365990832654	0.0
3	3.515086752341048E-4	0.0	0.0	1.5476926970557634	0.0
4	3.515086752341048E-4	0.0	0.0	3.446191051995163	0.0
5	3.515086752341048E-4	0.0	0.0	3.7769607153904556	0.0
6	3.515086752341048E-4	0.0	0.0	5.246618486544248	0.0
7	3.515086752341048E-4	0.0	0.0	6.355628356907848	0.0
8	3.515086752341048E-4	0.0	0.0	7.4270267990214	0.0
9	3.515086752341048E-4	0.0	0.0	9.542054497905008	0.0
10	3.515086752341048E-4	0.0	0.0	11.645833919181126	0.0
11	3.515086752341048E-4	0.0	0.0	13.874750428840583	0.0
12	3.515086752341048E-4	0.0	0.0	14.700444306965496	0.0
13	3.515086752341048E-4	0.0	0.0	15.144751272461404	0.0
14	3.515086752341048E-4	0.0	0.0	15.655493377576558	0.0
15	3.515086752341048E-4	0.0	0.0	15.982044936869041	0.0
16	3.515086752341048E-4	0.0	0.0	16.568009898484295	0.0
17	3.515086752341048E-4	0.0	0.0	17.188071201597257	0.0
18	3.515086752341048E-4	0.0	0.0	18.412727426112877	0.0
19	3.515086752341048E-4	0.0	0.0	18.780757009082983	0.0
20	3.515086752341048E-4	0.0	0.0	19.237718286887322	0.0
21	3.515086752341048E-4	0.0	0.0	19.617699164815388	0.0
22	3.515086752341048E-4	0.0	0.0	19.966747279322853	0.0
23	0.0017575433761705238	0.0	0.0	20.326340654087343	0.0
24	0.0017575433761705238	0.0	0.0	20.570287674699813	0.0
25	0.0017575433761705238	0.0	0.0	20.812125643260877	0.0
26	0.0017575433761705238	0.0	0.0	21.01213407946908	0.0
27	0.0017575433761705238	0.0	0.0	21.28947442422879	0.0
28	0.0017575433761705238	0.0	0.0	21.523227693259468	0.0
29	0.0017575433761705238	0.0	0.0	21.75873850566632	0.0
30	0.0017575433761705238	0.0	0.0	22.102865498720508	0.0
31	0.0017575433761705238	0.0	0.0	22.359115322966172	0.0
32	0.0017575433761705238	0.0	0.0	22.575293158235144	0.0
33	0.0017575433761705238	0.0	0.0	22.807288883889655	0.0
34	0.0017575433761705238	0.0	0.0	23.048072326425014	0.0
35	0.0017575433761705238	0.0	0.0	23.29412839908889	0.0
36	0.0017575433761705238	0.0	0.0	23.510306234357863	0.0
37	0.0017575433761705238	0.0	0.0	23.742301960012373	0.0
38	0.0017575433761705238	0.0	0.0	23.990818593402885	0.0
39	0.0017575433761705238	0.0	0.0	24.23757768341723	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCAG	30	2.1597225E-6	45.000004	14
AAATCCG	30	2.1597225E-6	45.000004	1
GCGACAG	30	2.1597225E-6	45.000004	1
AGTCAGC	30	2.1597225E-6	45.000004	11
TAGCGAG	30	2.1597225E-6	45.000004	1
GCTGCGC	30	2.1597225E-6	45.000004	9
TTCAATC	30	2.1597225E-6	45.000004	37
CTGCGCC	30	2.1597225E-6	45.000004	10
GTCAGAC	30	2.1597225E-6	45.000004	9
TGACCTT	30	2.1597225E-6	45.000004	34
TGCGCCG	30	2.1597225E-6	45.000004	11
GTTGTCA	30	2.1597225E-6	45.000004	10
TTGACAG	30	2.1597225E-6	45.000004	1
TCATGTA	30	2.1597225E-6	45.000004	34
CTACCGC	30	2.1597225E-6	45.000004	2
CGCCGTG	30	2.1597225E-6	45.000004	13
TACAGGT	30	2.1597225E-6	45.000004	39
CACCGAG	55	1.8189894E-12	45.000004	1
TCAAGAC	30	2.1597225E-6	45.000004	34
GTGCGGA	30	2.1597225E-6	45.000004	29
>>END_MODULE