SRR3553331_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553331_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	279052
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17020	6.099221650445078	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3953	1.4165818557114802	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3084	1.10517036251308	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGT	2248	0.8055846222209481	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC	2174	0.779066267219013	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	1902	0.6815933947794677	RNA PCR Primer, Index 33 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1902	0.6815933947794677	No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	1825	0.6539999713315081	TruSeq Adapter, Index 1 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	609	0.2182388945429526	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	533	0.19100382724366785	No Hit
TGAGTGGAGGGATAGTTTAAAGTTTTCACCATGTTATATTTTCTTTTGAGA	532	0.19064547109499305	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	445	0.15946848616028553	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	433	0.15516821237618794	RNA PCR Primer, Index 33 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	424	0.15194300703811475	No Hit
AACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	420	0.15050958244341556	Illumina Paired End PCR Primer 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	420	0.15050958244341556	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTAT	382	0.1368920487937732	No Hit
AATGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	321	0.11503232372461047	RNA PCR Primer, Index 33 (95% over 21bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	316	0.11324054298123647	No Hit
AATCACGGGAACTCTAATTACTTGTTTTTCTTTTGGCTTTTGTGAATTTGG	296	0.1060734200077405	No Hit
AGATTAGGGGAAAGGAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGA	289	0.1035649269670169	No Hit
CCAACAGGTAAGGAAAGAAGGGACAGAAAGAGGGAAGGAGGAAGCGAATGA	288	0.1032065708183421	No Hit
TTTTCAGGGGTTGTCAAGGTTGGTAGCAAGTGCCTTAACACCATGGGTCAT	285	0.10213150237231772	No Hit
GATGGAGGGACATTTTGTAATGTAGATTTTTTTCCATGAAAGATTTCTGAA	284	0.1017731462236429	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCACG	45	3.8198777E-10	45.000004	1
TTCACGG	75	0.0	45.000004	2
AACGGGA	115	0.0	45.000004	4
GGAACGT	45	3.8198777E-10	45.000004	8
CGGGATA	45	3.8198777E-10	45.000004	6
AGGGCGT	45	3.8198777E-10	45.000004	6
CCTTCCG	40	6.7830115E-9	45.0	21
GCCCCTC	20	7.0240983E-4	45.0	36
GTGTTCG	20	7.0240983E-4	45.0	9
GTGTTAC	20	7.0240983E-4	45.0	22
GTTTGCA	25	3.88319E-5	45.0	21
AACAATC	25	3.88319E-5	45.0	33
AACGTAT	25	3.88319E-5	45.0	10
CGGAACT	20	7.0240983E-4	45.0	6
GAGTGCA	25	3.88319E-5	45.0	9
ACAGTTC	20	7.0240983E-4	45.0	11
CTGTCGA	25	3.88319E-5	45.0	32
CCTTACG	25	3.88319E-5	45.0	20
CCGGGTC	25	3.88319E-5	45.0	5
TCGCAAC	20	7.0240983E-4	45.0	24

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content