##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553331_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	279052
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.493768186574545	31.0	31.0	34.0	30.0	34.0
2	31.68466450697361	31.0	31.0	34.0	30.0	34.0
3	31.868920487937732	31.0	31.0	34.0	30.0	34.0
4	35.6164406633889	37.0	35.0	37.0	33.0	37.0
5	35.33338947579663	37.0	35.0	37.0	33.0	37.0
6	32.47582887777188	37.0	35.0	37.0	25.0	37.0
7	33.94613548729269	37.0	35.0	37.0	25.0	37.0
8	35.000483780800714	37.0	35.0	37.0	32.0	37.0
9	36.83644983730631	39.0	37.0	39.0	32.0	39.0
10	36.524482892077465	38.0	35.0	39.0	32.0	39.0
11	36.60570072961312	38.0	35.0	39.0	32.0	39.0
12	36.503726903946216	38.0	35.0	39.0	32.0	39.0
13	36.498803090463426	38.0	35.0	39.0	32.0	39.0
14	37.533108524576065	39.0	36.0	40.0	32.0	41.0
15	37.67460544988031	39.0	36.0	41.0	32.0	41.0
16	37.58045095537749	39.0	36.0	41.0	32.0	41.0
17	37.47463913535829	39.0	36.0	40.0	32.0	41.0
18	37.13010836689936	38.0	36.0	40.0	32.0	41.0
19	36.85182689964594	38.0	35.0	40.0	32.0	41.0
20	36.79239353238823	38.0	35.0	40.0	31.0	41.0
21	36.7325301377521	38.0	35.0	40.0	31.0	41.0
22	36.60772902541462	38.0	35.0	40.0	31.0	41.0
23	36.37959591760676	38.0	35.0	40.0	31.0	41.0
24	36.30791035362585	38.0	35.0	40.0	31.0	41.0
25	36.28376073276665	38.0	35.0	40.0	31.0	41.0
26	36.24753092613563	38.0	35.0	40.0	31.0	41.0
27	36.14853145650273	38.0	35.0	40.0	30.0	41.0
28	36.01353511173545	38.0	35.0	40.0	30.0	41.0
29	35.72516233533535	38.0	34.0	40.0	29.0	41.0
30	35.72516233533535	38.0	35.0	40.0	29.0	41.0
31	35.19145177242951	38.0	34.0	40.0	26.0	41.0
32	35.318911887390165	38.0	34.0	40.0	27.0	41.0
33	35.1601135272279	38.0	34.0	40.0	26.0	41.0
34	35.109789573269495	38.0	34.0	40.0	26.0	41.0
35	34.97788942562676	38.0	34.0	40.0	25.0	41.0
36	34.82911787050442	37.0	34.0	40.0	25.0	41.0
37	34.72830870232072	37.0	33.0	40.0	24.0	41.0
38	34.4921842523974	37.0	33.0	40.0	23.0	41.0
39	34.28346329716325	37.0	33.0	40.0	23.0	41.0
40	34.15517896306065	37.0	33.0	40.0	22.0	41.0
41	34.17067069936786	37.0	33.0	40.0	22.0	41.0
42	34.11907816464315	37.0	33.0	40.0	22.0	41.0
43	34.02988331923799	37.0	33.0	40.0	22.0	41.0
44	33.91043963132319	37.0	33.0	40.0	21.0	41.0
45	33.69102532861259	36.0	33.0	40.0	21.0	41.0
46	33.288989148975816	36.0	32.0	40.0	20.0	41.0
47	33.31392356980061	36.0	32.0	40.0	20.0	41.0
48	33.29728509381764	36.0	32.0	40.0	20.0	41.0
49	33.164732737984316	36.0	32.0	40.0	20.0	41.0
50	32.96678755214082	35.0	32.0	39.0	18.0	41.0
51	31.79462967475596	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	4.0
14	9.0
15	7.0
16	44.0
17	107.0
18	292.0
19	549.0
20	1048.0
21	1541.0
22	2160.0
23	2731.0
24	3215.0
25	3541.0
26	3835.0
27	4301.0
28	4885.0
29	5814.0
30	7391.0
31	9394.0
32	12217.0
33	15705.0
34	22725.0
35	34311.0
36	28188.0
37	34699.0
38	42546.0
39	37784.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.079426056792283	40.83432478534467	18.4915356277683	22.594713530094747
2	17.968335650703096	43.32561673093187	17.484554849992115	21.221492768372922
3	16.24894284936141	41.43600475896965	21.33043303757006	20.984619354098875
4	19.23691641701188	40.19179221077076	17.692043060074823	22.87924831214254
5	15.071384544816018	45.756704843541705	15.871593824806846	23.300316786835428
6	15.307541246792713	49.26787838825739	17.369522526267506	18.055057838682394
7	69.17241231025042	21.45514097730889	5.334847985321732	4.03759872711896
8	70.10198815991285	18.427748233304186	3.933317087854593	7.536946518928373
9	65.7443773920273	20.32560239668592	8.901566733082007	5.02845347820478
10	29.633545002365153	40.537247538093254	13.926078293651361	15.903129165890228
11	21.064174419104685	40.46020096612818	23.264481171967947	15.211143442799191
12	22.287602310680445	33.921634677407795	28.89963160987916	14.891131402032595
13	21.60600891590098	36.69746140504279	24.445623038000086	17.250906641056147
14	14.799750584120522	40.6830984906039	27.337557157805715	17.17959376746986
15	14.175494173129021	40.05561687427433	26.237045425225407	19.531843527371244
16	15.837191634534065	40.63472041053281	25.388099709014806	18.139988245918325
17	15.975875464071212	38.66447830511876	24.316973180625833	21.042673050184195
18	17.726445250347606	38.0624399753451	24.111993463583847	20.09912131072345
19	17.8217679858951	41.83843871393145	25.119690953657383	15.220102346516063
20	17.326878144575204	40.515029456875425	23.020082278571735	19.13801011997764
21	17.45875320728753	39.22351389705144	24.084041683987216	19.233691211673808
22	17.160600891590096	39.78505798202486	23.333643908662186	19.72069721772286
23	16.712297349597925	39.72557086134484	21.953614380115535	21.6085174089417
24	14.650316070123132	42.004715966916564	22.82406146524662	20.520906497713685
25	15.207918237461119	40.47310178748047	23.40101486461305	20.917965110445365
26	18.758152602382353	39.294826770637734	22.46713874116652	19.479881885813395
27	15.827157662371171	39.66536702836747	23.48630362799765	21.021171681263706
28	18.065450166993966	38.79922021702048	24.734458093831975	18.40087152215358
29	18.11203646632169	41.89720912231412	21.796296030847298	18.194458380516892
30	20.482920745954157	39.39588320456402	20.435976090477762	19.68521995900406
31	18.70654931697318	41.23568367186044	19.546894485615585	20.510872525550795
32	20.574659920014906	38.52686954402763	19.79523529664722	21.103235239310237
33	18.02388085374769	38.92966185513811	23.592018691856715	19.454438599257486
34	17.999154279489126	39.21240485644253	22.44850422143543	20.339936642632914
35	19.212548198901995	38.62720926565658	21.623568367186046	20.536674168255377
36	18.654587675415335	39.523816349640924	23.963275661883806	17.85832031305993
37	20.23457993492252	38.59674899301922	20.893596892335477	20.275074179722775
38	20.465361294669094	36.94723564066912	21.943222051803964	20.644181012857818
39	19.76298324326649	37.51523013631868	22.550994079956425	20.17079254045841
40	19.618565715350545	38.52758625632499	21.322907558447888	20.530940469876583
41	16.446038731132546	36.12229978641974	25.356564367931423	22.07509711451629
42	20.472886773791267	35.240743660679726	23.713501426257473	20.572868139271534
43	18.85490876252455	35.34968392987687	25.358356148674797	20.437051158923786
44	21.024038530453108	31.441810128578183	25.151226294740763	22.382925046227946
45	19.95076186517208	34.427991915485286	25.14334245946992	20.477903759872714
46	18.275088513968722	33.56220346028697	28.795349970614797	19.36735805512951
47	19.123317517881972	34.88597107349168	25.73534681708069	20.25536459154566
48	18.93338875908433	32.91357883118559	28.231655748749336	19.921376660980748
49	19.42182818972808	31.792640798130815	26.1560569356249	22.629474076516203
50	19.186029843900062	32.11193612660006	24.6885885068016	24.01344552269828
51	17.592778406891902	31.487321359459884	29.57584966242851	21.3440505712197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19566.0
1	14770.0
2	9974.0
3	6178.0
4	2382.0
5	1938.5
6	1495.0
7	1542.5
8	1590.0
9	1696.0
10	1802.0
11	1964.0
12	2126.0
13	2388.5
14	2651.0
15	2327.5
16	2004.0
17	1939.5
18	1875.0
19	1900.0
20	1925.0
21	1830.5
22	1736.0
23	1989.0
24	2242.0
25	1989.5
26	2012.0
27	2287.0
28	3211.0
29	4135.0
30	4896.0
31	5657.0
32	5513.0
33	5369.0
34	5518.5
35	5668.0
36	6955.5
37	8243.0
38	8295.0
39	8347.0
40	9221.5
41	10096.0
42	11376.0
43	12656.0
44	17477.5
45	22299.0
46	23307.5
47	24316.0
48	24383.0
49	24450.0
50	23114.0
51	21778.0
52	19007.0
53	16236.0
54	14463.0
55	12690.0
56	11463.0
57	10236.0
58	9085.0
59	7934.0
60	7511.0
61	7088.0
62	6232.5
63	5377.0
64	4672.5
65	3968.0
66	3144.0
67	2320.0
68	2175.0
69	2030.0
70	1645.0
71	1260.0
72	921.5
73	583.0
74	396.0
75	350.5
76	492.0
77	283.5
78	75.0
79	52.0
80	29.0
81	29.5
82	30.0
83	22.0
84	14.0
85	40.5
86	67.0
87	36.5
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	279052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.09115146997692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.80423445857667	25.93100927425713
2	8.169646346774464	5.2434671674096585
3	3.0708758137820906	2.9564382265670917
4	1.5644716418577125	2.008227857173573
5	0.9089792408794989	1.4585095251064317
6	0.6867595001730857	1.3223341886100082
7	0.4835233554064165	1.0861774866333158
8	0.3964221505064153	1.017731462236429
9	0.2970374423512859	0.8579046199274687
>10	2.7716050072025995	19.322205180396484
>50	0.5706245603064175	12.944540802431087
>100	0.26353697892820854	13.028754497369665
>500	0.0033500463423077353	0.5998881928816134
>1k	0.007816774798718049	6.123589868554965
>5k	0.0	0.0
>10k+	0.0011166821141025784	6.099221650445078
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17020	6.099221650445078	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3953	1.4165818557114802	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3084	1.10517036251308	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGT	2248	0.8055846222209481	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC	2174	0.779066267219013	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	1902	0.6815933947794677	RNA PCR Primer, Index 33 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1902	0.6815933947794677	No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	1825	0.6539999713315081	TruSeq Adapter, Index 1 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	609	0.2182388945429526	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	533	0.19100382724366785	No Hit
TGAGTGGAGGGATAGTTTAAAGTTTTCACCATGTTATATTTTCTTTTGAGA	532	0.19064547109499305	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	445	0.15946848616028553	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	433	0.15516821237618794	RNA PCR Primer, Index 33 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	424	0.15194300703811475	No Hit
AACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	420	0.15050958244341556	Illumina Paired End PCR Primer 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	420	0.15050958244341556	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTAT	382	0.1368920487937732	No Hit
AATGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	321	0.11503232372461047	RNA PCR Primer, Index 33 (95% over 21bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	316	0.11324054298123647	No Hit
AATCACGGGAACTCTAATTACTTGTTTTTCTTTTGGCTTTTGTGAATTTGG	296	0.1060734200077405	No Hit
AGATTAGGGGAAAGGAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGA	289	0.1035649269670169	No Hit
CCAACAGGTAAGGAAAGAAGGGACAGAAAGAGGGAAGGAGGAAGCGAATGA	288	0.1032065708183421	No Hit
TTTTCAGGGGTTGTCAAGGTTGGTAGCAAGTGCCTTAACACCATGGGTCAT	285	0.10213150237231772	No Hit
GATGGAGGGACATTTTGTAATGTAGATTTTTTTCCATGAAAGATTTCTGAA	284	0.1017731462236429	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8073764029643221	0.0
2	0.0	0.0	0.0	1.028482146696673	0.0
3	0.0	0.0	0.0	1.4018892536158136	0.0
4	0.0	0.0	0.0	3.174677121110044	0.0
5	0.0	0.0	0.0	3.49397244957929	0.0
6	0.0	0.0	0.0	4.883319237991485	0.0
7	0.0	0.0	0.0	5.972005217665525	0.0
8	0.0	0.0	0.0	7.015896678755214	0.0
9	0.0	0.0	0.0	9.073219328297235	0.0
10	0.0	0.0	0.0	11.135558963920703	0.0
11	0.0	0.0	0.0	13.349483250433611	0.0
12	0.0	0.0	0.0	14.143600475896966	0.0
13	0.0	0.0	0.0	14.580436621131545	0.0
14	0.0	0.0	0.0	15.040565916029987	0.0
15	0.0	0.0	0.0	15.334776314091997	0.0
16	0.0	0.0	0.0	15.882702865415764	0.0
17	0.0	0.0	0.0	16.487608044378824	0.0
18	0.0	0.0	0.0	17.678783882573857	0.0
19	0.0	0.0	0.0	18.021372360706966	0.0
20	0.0	0.0	0.0	18.458925218238896	0.0
21	0.0	0.0	0.0	18.857058899416597	0.0
22	0.0	0.0	0.0	19.194988747616932	0.0
23	0.0017917807433739947	0.0	0.0	19.54796955406161	0.0
24	0.0017917807433739947	0.0	0.0	19.809927898742888	0.0
25	0.0017917807433739947	0.0	0.0	20.043576107678856	0.0
26	0.0017917807433739947	0.0	0.0	20.255722947694338	0.0
27	0.0017917807433739947	0.0	0.0	20.523773346903088	0.0
28	0.0017917807433739947	0.0	0.0	20.747745939824835	0.0
29	0.0017917807433739947	0.0	0.0	20.98461935409888	0.0
30	0.0017917807433739947	0.0	0.0	21.331149749867407	0.0
31	0.0017917807433739947	0.0	0.0	21.56479795880338	0.0
32	0.0017917807433739947	0.0	0.0	21.799879592334044	0.0
33	0.0017917807433739947	0.0	0.0	22.03531958201339	0.0
34	0.0017917807433739947	0.0	0.0	22.266459297908632	0.0
35	0.0017917807433739947	0.0	0.0	22.497240657655205	0.0
36	0.0017917807433739947	0.0	0.0	22.702937086994538	0.0
37	0.0017917807433739947	0.0	0.0	22.937660364376534	0.0
38	0.0017917807433739947	0.0	0.0	23.183492682367444	0.0
39	0.0017917807433739947	0.0	0.0	23.445451027048723	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCACG	45	3.8198777E-10	45.000004	1
TTCACGG	75	0.0	45.000004	2
AACGGGA	115	0.0	45.000004	4
GGAACGT	45	3.8198777E-10	45.000004	8
CGGGATA	45	3.8198777E-10	45.000004	6
AGGGCGT	45	3.8198777E-10	45.000004	6
CCTTCCG	40	6.7830115E-9	45.0	21
GCCCCTC	20	7.0240983E-4	45.0	36
GTGTTCG	20	7.0240983E-4	45.0	9
GTGTTAC	20	7.0240983E-4	45.0	22
GTTTGCA	25	3.88319E-5	45.0	21
AACAATC	25	3.88319E-5	45.0	33
AACGTAT	25	3.88319E-5	45.0	10
CGGAACT	20	7.0240983E-4	45.0	6
GAGTGCA	25	3.88319E-5	45.0	9
ACAGTTC	20	7.0240983E-4	45.0	11
CTGTCGA	25	3.88319E-5	45.0	32
CCTTACG	25	3.88319E-5	45.0	20
CCGGGTC	25	3.88319E-5	45.0	5
TCGCAAC	20	7.0240983E-4	45.0	24
>>END_MODULE