FastQCFastQC Report
Sat 18 Jun 2016
SRR3553328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275755
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220261.7265070487672007No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50830.3984307331736893No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38580.3024091616336993No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT35560.27873690481322827No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20520.16084593044902823No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG16480.12917840807992131No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCG302.1656906E-645.0000049
CAGGTCG253.891049E-545.032
ACTATCG207.033578E-445.01
TCGCATA253.891049E-545.028
CGTATCA207.033578E-445.016
CGTATAG453.8562575E-1045.01
CGTATAA253.891049E-545.027
CGAAACT207.033578E-445.037
CGACGGT253.891049E-545.028
ATAGCGC207.033578E-445.010
ATTCGGC207.033578E-445.036
CGACATA207.033578E-445.022
ATATGCG207.033578E-445.01
CGCGACT253.891049E-545.032
CATTACG351.2120654E-745.01
CGTTAGA253.891049E-545.039
ATTCCGC253.891049E-545.022
TACGCTA253.891049E-545.012
GTAGCGT253.891049E-545.026
CGCTATA207.033578E-445.025