##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553328_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1275755
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68844880090613	31.0	31.0	34.0	30.0	34.0
2	31.887880509972526	33.0	31.0	34.0	30.0	34.0
3	32.068176099642955	33.0	31.0	34.0	30.0	34.0
4	35.758008395028824	37.0	35.0	37.0	35.0	37.0
5	35.510947634929906	37.0	35.0	37.0	33.0	37.0
6	34.175602682333206	37.0	35.0	37.0	32.0	37.0
7	34.961460468506885	37.0	35.0	37.0	32.0	37.0
8	35.635270094963374	37.0	35.0	37.0	33.0	37.0
9	37.35530097863618	39.0	37.0	39.0	35.0	39.0
10	36.750793843645525	39.0	37.0	39.0	32.0	39.0
11	36.75572425740052	39.0	37.0	39.0	32.0	39.0
12	36.684002022331875	39.0	35.0	39.0	32.0	39.0
13	36.64632942845609	39.0	35.0	39.0	32.0	39.0
14	37.84311799679406	40.0	37.0	41.0	33.0	41.0
15	37.843574197240066	40.0	37.0	41.0	33.0	41.0
16	37.77416980533096	40.0	37.0	41.0	33.0	41.0
17	37.750522631696526	39.0	37.0	41.0	33.0	41.0
18	37.63736375714773	39.0	36.0	41.0	33.0	41.0
19	37.6181084926181	39.0	36.0	41.0	33.0	41.0
20	37.509764805938445	39.0	36.0	41.0	32.0	41.0
21	37.47899714286834	39.0	36.0	41.0	32.0	41.0
22	37.45973403984307	39.0	36.0	41.0	32.0	41.0
23	37.27565402447962	39.0	35.0	41.0	32.0	41.0
24	37.093643371964056	39.0	35.0	41.0	31.0	41.0
25	37.133106278243076	39.0	35.0	41.0	31.0	41.0
26	37.05214088912056	39.0	35.0	41.0	31.0	41.0
27	36.99172803555542	39.0	35.0	41.0	31.0	41.0
28	36.93790500527139	39.0	35.0	40.0	31.0	41.0
29	36.727083962046	39.0	35.0	40.0	31.0	41.0
30	36.75209660162022	39.0	35.0	40.0	31.0	41.0
31	36.70652829109037	39.0	35.0	40.0	31.0	41.0
32	36.59294221852942	39.0	35.0	40.0	30.0	41.0
33	36.55063550603368	39.0	35.0	40.0	30.0	41.0
34	36.46509400315892	39.0	35.0	40.0	30.0	41.0
35	36.33479860945087	39.0	35.0	40.0	30.0	41.0
36	36.29822105341543	38.0	35.0	40.0	30.0	41.0
37	36.21290765076367	38.0	35.0	40.0	30.0	41.0
38	36.106797935340246	38.0	35.0	40.0	30.0	41.0
39	36.032304008214744	38.0	35.0	40.0	30.0	41.0
40	35.86523901532818	38.0	35.0	40.0	29.0	41.0
41	35.77159564336412	38.0	35.0	40.0	29.0	41.0
42	35.665965643873626	38.0	34.0	40.0	28.0	41.0
43	35.57679256597074	38.0	34.0	40.0	28.0	41.0
44	35.44564199238882	38.0	34.0	40.0	28.0	41.0
45	35.29812150452085	38.0	34.0	40.0	27.0	41.0
46	35.10347519704018	37.0	34.0	40.0	26.0	41.0
47	34.990853259442446	37.0	34.0	40.0	26.0	41.0
48	34.877222507456366	37.0	34.0	40.0	26.0	41.0
49	34.662973690089395	37.0	33.0	40.0	26.0	41.0
50	34.585719044800925	37.0	33.0	40.0	26.0	41.0
51	33.40118909978797	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	11.0
10	15.0
11	36.0
12	22.0
13	60.0
14	59.0
15	136.0
16	207.0
17	418.0
18	791.0
19	1402.0
20	2083.0
21	3142.0
22	4380.0
23	5820.0
24	7740.0
25	9474.0
26	11208.0
27	13723.0
28	16593.0
29	21197.0
30	26754.0
31	34808.0
32	44801.0
33	60660.0
34	88197.0
35	116281.0
36	129417.0
37	175681.0
38	245924.0
39	254663.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.94426045753299	32.083119407723274	18.572531559743055	27.400088575000687
2	24.073587797029994	30.75582694169335	19.989888340629665	25.180696920646987
3	21.630955786965366	31.077205262765972	20.429157636066485	26.86268131420218
4	20.225121594663552	33.289307116178264	19.273057914725005	27.21251337443318
5	17.626503521444164	36.93475628157444	16.04203001359979	29.396710183381607
6	18.974959925691063	41.41767032071205	18.404239058439902	21.203130695156986
7	83.95514812797128	8.152349001179694	4.296984922653644	3.5955179481953823
8	84.75765331117651	7.617998753679194	4.054540252634714	3.569807682509573
9	78.65985240112717	10.815556278439043	5.915947811296056	4.6086435091377265
10	35.202056821254864	35.87914607428542	12.8236220904484	16.09517501401131
11	25.441562055410326	31.180438250291004	24.036590097628462	19.341409596670207
12	26.126097879295006	26.706773636003778	27.89336510536898	19.27376337933224
13	24.198141492684723	27.238537179944426	27.114610563940566	21.44871076343028
14	21.33685543070574	28.901552414060696	27.224192732930696	22.537399422302872
15	19.268276432387097	31.638911860035822	27.219646405461862	21.87316530211522
16	21.534071980905427	29.669489831511537	27.325309326634034	21.47112886094901
17	21.283240120556062	29.574565649360572	27.073458461852002	22.068735768231363
18	21.13454385834271	30.14160242366285	26.337266951726622	22.38658676626782
19	21.680495079384364	30.655886122335403	26.078596595741345	21.58502220253889
20	23.318348742509336	29.919302687428228	25.87354154990574	20.88880702015669
21	22.949469137883057	30.258396008638023	26.165525512343674	20.62660934113525
22	23.000027434734726	27.792953976272873	25.34765687769203	23.859361711300366
23	21.127254057401306	30.85741384513484	26.166230976950906	21.84910112051295
24	19.83750798546743	30.288260677010868	27.567440456827523	22.306790880694177
25	22.2155507914921	30.68951326861349	24.718225678127855	22.37671026176656
26	22.833851327253274	30.00505582968517	24.742603399555556	22.418489443506
27	21.037111357588252	29.15489259301355	27.260328197812278	22.547667851585924
28	20.738072749077997	29.851107775395747	28.02324897805613	21.387570497470126
29	20.71204894356675	31.52784037687487	25.27373986384533	22.486370815713048
30	22.86340245580068	29.299747992365305	26.72636987509357	21.11047967674044
31	23.22867635243444	29.123068300731724	24.318540785652417	23.32971456118142
32	21.861877868399496	30.994979443545194	24.821928975391042	22.321213712664264
33	21.298290032177025	29.64958005259631	25.82737281061019	23.22475710461648
34	20.458081684963023	30.04409153795204	26.318062637418628	23.179764139666315
35	21.887039439390794	29.21211361115575	26.6744790339838	22.226367915469663
36	22.79418853933553	30.238564614679152	25.9842211082849	20.98302573770042
37	21.129292066266643	31.620648165204134	25.655631371227233	21.59442839730199
38	21.80928156268249	31.03103652347042	25.88263420484341	21.27704770900369
39	21.02221821588001	29.456047595345503	25.79688106258647	23.724853126188023
40	21.655019968567633	29.735803504591402	25.951456196526763	22.657720330314206
41	21.120748106023495	28.51770128276981	27.408005455592964	22.953545155613735
42	21.90044326692821	29.43958675451007	24.92947313551583	23.73049684304588
43	20.972208613722852	28.513782034951852	26.884158792244595	23.6298505590807
44	22.376239952028406	27.345924570156495	26.226117083609314	24.051718394205786
45	22.454232983605788	27.735811343087036	26.946161292724703	22.863794380582476
46	20.998154034277743	29.058400711735406	27.58241198349213	22.361033270494726
47	21.10123025189006	30.31177616391862	25.877304027810982	22.70968955638034
48	21.004503215742833	28.804825377913474	27.34655164980737	22.844119756536326
49	20.237192877942864	29.84671821783963	27.550352536341226	22.365736367876277
50	21.554295299646093	27.857308025443757	27.873298556541027	22.715098118369124
51	22.326387119783973	27.26957762266266	26.99585735505642	23.408177902496952
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25210.0
1	19004.5
2	12799.0
3	8015.0
4	3231.0
5	2770.0
6	2309.0
7	2431.5
8	2554.0
9	2756.5
10	2959.0
11	3111.5
12	3264.0
13	3415.5
14	3567.0
15	4071.0
16	4575.0
17	4625.5
18	4676.0
19	5201.0
20	5726.0
21	5705.0
22	5684.0
23	6492.5
24	7301.0
25	8593.5
26	12043.0
27	14200.0
28	16643.5
29	19087.0
30	23355.0
31	27623.0
32	30078.0
33	32533.0
34	37817.5
35	43102.0
36	44309.0
37	45516.0
38	46834.5
39	48153.0
40	52952.0
41	57751.0
42	62771.5
43	67792.0
44	70899.5
45	74007.0
46	83602.5
47	93198.0
48	93460.0
49	93722.0
50	88862.5
51	84003.0
52	81283.0
53	78563.0
54	70256.0
55	61949.0
56	60915.0
57	59881.0
58	58023.5
59	56166.0
60	53226.0
61	50286.0
62	45507.0
63	40728.0
64	37783.5
65	34839.0
66	31490.5
67	28142.0
68	24922.0
69	21702.0
70	19192.0
71	16682.0
72	14062.0
73	11442.0
74	9532.0
75	6918.0
76	6214.0
77	4842.0
78	3470.0
79	2548.5
80	1627.0
81	1268.5
82	910.0
83	740.0
84	570.0
85	402.5
86	235.0
87	210.5
88	186.0
89	128.5
90	71.0
91	47.5
92	24.0
93	15.5
94	7.0
95	8.0
96	9.0
97	4.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1275755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.825219636038867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0304375116871	15.271453423293641
2	9.658774437043604	3.8297464925869424
3	3.590459242022455	2.135449292020224
4	1.7371116905061261	1.377544831864589
5	1.0268361560220132	1.0178626161681144
6	0.7130384884998832	0.8481686786075615
7	0.5017399886218178	0.6962973832227827
8	0.39710058443770924	0.6298085043261586
9	0.28027530649211574	0.5000867558787875
>10	2.727168940852756	12.423809501707641
>50	1.101911952424645	16.10138953187044
>100	1.2238066588852747	40.85224203263878
>500	0.008099316074687456	1.0750209709515273
>1k	0.0024297948224062367	1.064664159506214
>5k	4.049658037343728E-4	0.40809048508936624
>10k+	4.049658037343728E-4	1.76836534026724
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22026	1.7265070487672007	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5083	0.3984307331736893	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3858	0.3024091616336993	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	3556	0.27873690481322827	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2052	0.16084593044902823	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1648	0.12917840807992131	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.838495635917554E-5	0.0	0.0	0.3314899804429534	0.0
2	7.838495635917554E-5	0.0	0.0	0.4380935210914321	0.0
3	7.838495635917554E-5	0.0	0.0	0.560844362749901	0.0
4	7.838495635917554E-5	0.0	0.0	1.1151827741219904	0.0
5	7.838495635917554E-5	0.0	0.0	1.1984275977754348	0.0
6	7.838495635917554E-5	0.0	0.0	1.6448299242409397	0.0
7	7.838495635917554E-5	0.0	0.0	1.9937997499519893	0.0
8	7.838495635917554E-5	0.0	0.0	2.2320116323275236	0.0
9	7.838495635917554E-5	0.0	0.0	2.7098463262930577	0.0
10	7.838495635917554E-5	0.0	0.0	3.3642823269358146	0.0
11	7.838495635917554E-5	0.0	0.0	4.050464234904037	0.0
12	7.838495635917554E-5	0.0	0.0	4.358360343482879	0.0
13	7.838495635917554E-5	0.0	0.0	4.477662247061544	0.0
14	7.838495635917554E-5	0.0	0.0	4.633491540303585	0.0
15	7.838495635917554E-5	0.0	0.0	4.773487072361072	0.0
16	7.838495635917554E-5	0.0	0.0	5.043915171800228	0.0
17	7.838495635917554E-5	0.0	0.0	5.381832718664634	0.0
18	7.838495635917554E-5	0.0	0.0	5.816085376894466	0.0
19	1.5676991271835108E-4	0.0	0.0	6.006325665978186	0.0
20	1.5676991271835108E-4	0.0	0.0	6.221492371184122	0.0
21	1.5676991271835108E-4	0.0	0.0	6.468013058933729	0.0
22	1.5676991271835108E-4	0.0	0.0	6.734365140642208	0.0
23	2.3515486907752664E-4	0.0	0.0	7.031522510199842	0.0
24	2.3515486907752664E-4	0.0	0.0	7.245983750798547	0.0
25	2.3515486907752664E-4	0.0	0.0	7.4286206991154256	0.0
26	2.3515486907752664E-4	0.0	0.0	7.599186364152992	0.0
27	2.3515486907752664E-4	0.0	0.0	7.776649905350165	0.0
28	2.3515486907752664E-4	0.0	0.0	7.972455526335386	0.0
29	2.3515486907752664E-4	0.0	0.0	8.167320527844296	0.0
30	2.3515486907752664E-4	0.0	0.0	8.394480131373188	0.0
31	2.3515486907752664E-4	0.0	0.0	8.581114712464384	0.0
32	2.3515486907752664E-4	0.0	0.0	8.779977346747613	0.0
33	2.3515486907752664E-4	0.0	0.0	8.992008653699182	0.0
34	2.3515486907752664E-4	0.0	0.0	9.189538743724304	0.0
35	2.3515486907752664E-4	0.0	0.0	9.413171024217032	0.0
36	2.3515486907752664E-4	0.0	0.0	9.604900627471576	0.0
37	2.3515486907752664E-4	0.0	0.0	9.814345230863292	0.0
38	2.3515486907752664E-4	0.0	0.0	10.014618794360986	0.0
39	2.3515486907752664E-4	0.0	0.0	10.219987380022026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCG	30	2.1656906E-6	45.000004	9
CAGGTCG	25	3.891049E-5	45.0	32
ACTATCG	20	7.033578E-4	45.0	1
TCGCATA	25	3.891049E-5	45.0	28
CGTATCA	20	7.033578E-4	45.0	16
CGTATAG	45	3.8562575E-10	45.0	1
CGTATAA	25	3.891049E-5	45.0	27
CGAAACT	20	7.033578E-4	45.0	37
CGACGGT	25	3.891049E-5	45.0	28
ATAGCGC	20	7.033578E-4	45.0	10
ATTCGGC	20	7.033578E-4	45.0	36
CGACATA	20	7.033578E-4	45.0	22
ATATGCG	20	7.033578E-4	45.0	1
CGCGACT	25	3.891049E-5	45.0	32
CATTACG	35	1.2120654E-7	45.0	1
CGTTAGA	25	3.891049E-5	45.0	39
ATTCCGC	25	3.891049E-5	45.0	22
TACGCTA	25	3.891049E-5	45.0	12
GTAGCGT	25	3.891049E-5	45.0	26
CGCTATA	20	7.033578E-4	45.0	25
>>END_MODULE