FastQCFastQC Report
Sat 18 Jun 2016
SRR3553326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397545
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA247741.772679949482843No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54680.39125752659127255No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43790.313335169887195No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT35000.25043916296076335No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27260.19505633092315453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG406.8175723E-945.0000041
TCCGCGA207.0338097E-445.00000429
CGAAACG253.891239E-545.0000041
ACGCATA207.0338097E-445.00000437
CTATACG253.891239E-545.0000041
CGCGATA207.0338097E-445.00000411
AATATCG406.8175723E-945.00000413
TCTACCG253.891239E-545.00000438
CGTAGGC207.0338097E-445.00000433
TTGCGTA207.0338097E-445.00000436
CGTACTG253.891239E-545.0000041
AATTCGC406.8175723E-945.00000437
ACTCGAT207.0338097E-445.00000415
CTCGACG253.891239E-545.0000041
ATCGGCG207.0338097E-445.0000041
CAATACG800.045.0000041
GTGCCGA406.8175723E-945.0000045
CTATCGT351.2121745E-745.014
CAATTCG351.2121745E-745.01
TAAGTCG351.2121745E-745.045