##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553326_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1397545
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79177557788837	31.0	31.0	34.0	30.0	34.0
2	31.983699272653116	33.0	31.0	34.0	30.0	34.0
3	32.15529732495197	34.0	31.0	34.0	30.0	34.0
4	35.84423685820492	37.0	35.0	37.0	35.0	37.0
5	35.60451005155469	37.0	35.0	37.0	33.0	37.0
6	34.26382048520799	37.0	35.0	37.0	32.0	37.0
7	35.07212504785177	37.0	35.0	37.0	32.0	37.0
8	35.757270070015636	37.0	35.0	37.0	33.0	37.0
9	37.505328987617574	39.0	37.0	39.0	35.0	39.0
10	36.8837089324494	39.0	37.0	39.0	32.0	39.0
11	36.8453273418745	39.0	37.0	39.0	32.0	39.0
12	36.78358120847629	39.0	37.0	39.0	32.0	39.0
13	36.72750644880845	39.0	37.0	39.0	32.0	39.0
14	37.9205699995349	40.0	37.0	41.0	33.0	41.0
15	37.92369404920772	40.0	37.0	41.0	33.0	41.0
16	37.84479211760623	40.0	37.0	41.0	33.0	41.0
17	37.81550075310634	39.0	37.0	41.0	33.0	41.0
18	37.70554436529772	39.0	37.0	41.0	33.0	41.0
19	37.693683566539896	39.0	36.0	41.0	33.0	41.0
20	37.59652533549904	39.0	36.0	41.0	33.0	41.0
21	37.56223520530645	39.0	36.0	41.0	33.0	41.0
22	37.55335463258786	39.0	36.0	41.0	33.0	41.0
23	37.434444686933155	39.0	36.0	41.0	32.0	41.0
24	37.236343731328866	39.0	35.0	41.0	32.0	41.0
25	37.2873846638212	39.0	35.0	41.0	32.0	41.0
26	37.2051733575663	39.0	35.0	41.0	32.0	41.0
27	37.17520079854316	39.0	35.0	41.0	32.0	41.0
28	37.11678479047186	39.0	35.0	41.0	32.0	41.0
29	36.94469587741361	39.0	35.0	40.0	31.0	41.0
30	36.9699973882773	39.0	35.0	40.0	31.0	41.0
31	36.886704184838415	39.0	35.0	40.0	31.0	41.0
32	36.838104676414716	39.0	35.0	40.0	31.0	41.0
33	36.77254471233484	39.0	35.0	40.0	31.0	41.0
34	36.695403725819205	39.0	35.0	40.0	31.0	41.0
35	36.569686128174766	39.0	35.0	40.0	31.0	41.0
36	36.54146091896862	39.0	35.0	40.0	31.0	41.0
37	36.47885756809262	39.0	35.0	40.0	30.0	41.0
38	36.39183997653027	38.0	35.0	40.0	30.0	41.0
39	36.365722033995326	38.0	35.0	40.0	30.0	41.0
40	36.221021863338926	38.0	35.0	40.0	30.0	41.0
41	36.115762283146516	38.0	35.0	40.0	30.0	41.0
42	36.002666103774835	38.0	35.0	40.0	30.0	41.0
43	35.931369651782234	38.0	35.0	40.0	30.0	41.0
44	35.78467813201006	38.0	35.0	40.0	29.0	41.0
45	35.662969707594385	38.0	34.0	40.0	29.0	41.0
46	35.50730316376217	38.0	34.0	40.0	28.0	41.0
47	35.397610094844886	38.0	34.0	40.0	28.0	41.0
48	35.27800679047902	37.0	34.0	40.0	28.0	41.0
49	35.111488359945476	37.0	34.0	40.0	27.0	41.0
50	35.025029605486765	37.0	34.0	40.0	27.0	41.0
51	33.858973414094	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	12.0
11	17.0
12	25.0
13	29.0
14	58.0
15	86.0
16	174.0
17	308.0
18	607.0
19	1071.0
20	1787.0
21	2578.0
22	3885.0
23	5194.0
24	6736.0
25	8447.0
26	10190.0
27	12580.0
28	15824.0
29	20571.0
30	26650.0
31	35069.0
32	46319.0
33	64629.0
34	97156.0
35	129495.0
36	144327.0
37	196523.0
38	281306.0
39	285845.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.647277905183735	30.232157104064626	20.760977285167918	28.35958770558372
2	21.973532158177374	32.06680285786862	19.84508548919713	26.114579494756878
3	21.375841207259874	32.09935994905352	20.48570886805076	26.03908997563585
4	20.849489640762908	32.91228547202415	19.190866841497055	27.04735804571588
5	18.665731693791614	35.81745131641557	16.5816485336787	28.935168456114113
6	18.922181396663433	40.74759667846116	17.72336490059354	22.606857024281865
7	85.85870222425753	7.357330175414745	3.653478063318176	3.130489537009542
8	86.99090190297987	6.942102043225799	3.1523850752569684	2.9146109785373637
9	80.83095714270381	9.953167876526338	5.526333677985324	3.6895413027845256
10	37.193149415582326	34.53069489712317	12.93332236171286	15.342833325581646
11	25.04928284956835	30.96780425675023	24.974580424959484	19.008332468721935
12	26.217474213710474	27.338940785448766	27.85362904235642	18.589955958484342
13	24.664966065493417	28.380481487179303	26.41703844956692	20.53751399776036
14	21.153594338643835	30.42714188094122	27.47038556897989	20.94887821143505
15	19.85238400194627	32.4661459917212	27.095370810957785	20.586099195374747
16	22.14926889652927	30.55343477312001	27.570489680117632	19.726806650233087
17	22.691004583036683	30.26972297850874	27.31203646394213	19.72723597451245
18	21.87593243866924	30.737471780872887	26.615600928771528	20.77099485168635
19	21.513153422608934	32.36346593490728	25.92095424476493	20.202426397718856
20	23.42221538483555	31.77786761785846	26.268134478675105	18.531782518630884
21	23.424648222418597	31.26961922514123	25.897198301306933	19.408534251133236
22	22.75504545470808	29.213585251279923	26.00402849282134	22.027340801190658
23	21.949489998533142	30.772962587966756	25.68811737725798	21.589430036242124
24	20.35705469233549	32.15338325420649	26.460257093689293	21.029304959768737
25	21.465140657367026	32.14765893048167	25.138653853722065	21.248546558429247
26	22.206225917591205	32.00383529689563	24.2775724574164	21.512366328096768
27	20.057243237248173	30.640873818016594	27.05394101799942	22.247941926735813
28	20.727060667098378	31.521131698800396	27.423088344203585	20.32871928989764
29	22.155350990486887	31.97449813780594	24.647435324086164	21.222715547621007
30	22.40536082916829	30.547066462976147	26.552848029938215	20.494724677917347
31	22.71053883774762	30.999288037236727	24.605218436615637	21.684954688400015
32	21.586496320333158	31.0442955325231	25.640748598435113	21.728459548708628
33	21.194666361369404	30.571609500946302	26.982816295718564	21.250907841965734
34	20.42617590131266	30.450683162259534	26.978594606971512	22.144546329456297
35	20.499518799036885	30.929021963514593	28.210540626598785	20.36091861084974
36	22.351552186155008	31.760551538590885	26.121162467040413	19.766733808213687
37	20.779223567040773	32.15581609178953	25.765467301589574	21.29949303958012
38	20.90150943261219	30.893674264513844	27.31969274692407	20.885123555949896
39	20.76791802768426	29.180741943908785	26.647156263304577	23.404183765102378
40	21.947987363555377	30.129119277017914	26.626477143848675	21.29641621557803
41	20.205360113627826	29.26739389429321	28.383057432855473	22.144188559223497
42	21.382638841683093	30.72301786346772	26.576460865303087	21.3178824295461
43	21.094347588091974	30.415907895631268	26.489522698732422	22.000221817544336
44	21.549860648494324	29.85950362957901	25.878164924921915	22.712470797004748
45	22.849067471888205	28.93030278094802	26.76235827826653	21.458271468897244
46	20.10160674611551	30.307074190813175	27.770626348346568	21.82069271472475
47	20.650855607511744	31.085653771434906	27.042063046270425	21.221427574782922
48	20.62037358367709	30.567173150059567	27.17765796450204	21.634795301761304
49	20.929773281003474	30.0327359763013	26.986966430419056	22.05052431227617
50	20.90222497307779	29.43268374184731	27.035408519940322	22.629682765134575
51	20.77528809447996	28.601368828910694	27.947364843350304	22.675978233259038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28532.0
1	21284.5
2	14037.0
3	8756.5
4	3476.0
5	3225.0
6	2974.0
7	3084.0
8	3194.0
9	4010.0
10	4826.0
11	5024.0
12	5222.0
13	5716.0
14	6210.0
15	6519.0
16	6828.0
17	6468.5
18	6109.0
19	6184.5
20	6260.0
21	6030.5
22	5801.0
23	7882.0
24	9963.0
25	10538.0
26	12192.0
27	13271.0
28	17346.5
29	21422.0
30	26936.0
31	32450.0
32	35055.0
33	37660.0
34	41461.5
35	45263.0
36	46724.0
37	48185.0
38	53920.5
39	59656.0
40	60775.0
41	61894.0
42	69359.5
43	76825.0
44	82081.0
45	87337.0
46	95920.0
47	104503.0
48	105315.5
49	106128.0
50	102196.0
51	98264.0
52	94097.0
53	89930.0
54	82256.0
55	74582.0
56	68059.5
57	61537.0
58	59977.5
59	58418.0
60	54701.5
61	50985.0
62	46174.0
63	41363.0
64	36182.0
65	31001.0
66	28514.5
67	26028.0
68	22504.5
69	18981.0
70	15425.5
71	11870.0
72	10359.5
73	8849.0
74	7870.0
75	5325.5
76	3760.0
77	3302.0
78	2844.0
79	2127.5
80	1411.0
81	992.5
82	574.0
83	492.0
84	410.0
85	377.0
86	344.0
87	288.5
88	233.0
89	146.0
90	59.0
91	53.0
92	47.0
93	29.5
94	12.0
95	12.0
96	12.0
97	6.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1397545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.35087979974559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.77861617928153	15.421615092556149
2	9.832972178499396	4.002192697577676
3	3.4739080586547835	2.120912560111532
4	1.7679902997327537	1.4392063230790981
5	1.0583813925225294	1.0769496250756676
6	0.7640040607614503	0.9328892884244282
7	0.6127663411436213	0.8729233907760616
8	0.45010843047132726	0.7328082052299301
9	0.3886281479474213	0.7118032253108144
>10	3.7061576494991817	16.862916871211116
>50	1.117444811755911	16.467536476155175
>100	1.0374986878382242	35.11309146383793
>500	0.009363056537241836	1.1752318008213105
>1k	0.001440470236498744	0.8535747558097908
>5k	3.60117559124686E-4	0.40073381684278664
>10k+	3.60117559124686E-4	1.8156144071805405
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24774	1.772679949482843	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5468	0.39125752659127255	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4379	0.313335169887195	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	3500	0.25043916296076335	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2726	0.19505633092315453	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.431080931204362E-4	0.0	0.0	0.2882196995445585	0.0
2	1.431080931204362E-4	0.0	0.0	0.40263461999434724	0.0
3	1.431080931204362E-4	0.0	0.0	0.5144735947679682	0.0
4	1.431080931204362E-4	0.0	0.0	1.0463348228500693	0.0
5	1.431080931204362E-4	0.0	0.0	1.1376377862609075	0.0
6	1.431080931204362E-4	0.0	0.0	1.6599107721039394	0.0
7	1.431080931204362E-4	0.0	0.0	2.056463298140668	0.0
8	1.431080931204362E-4	0.0	0.0	2.344182119359305	0.0
9	1.431080931204362E-4	0.0	0.0	2.8582979438944722	0.0
10	1.431080931204362E-4	0.0	0.0	3.580421381780193	0.0
11	1.431080931204362E-4	0.0	0.0	4.345334139508925	0.0
12	1.431080931204362E-4	0.0	0.0	4.69480410290903	0.0
13	1.431080931204362E-4	0.0	0.0	4.832903412770251	0.0
14	1.431080931204362E-4	0.0	0.0	5.003989138095732	0.0
15	1.431080931204362E-4	0.0	0.0	5.150746487590739	0.0
16	1.431080931204362E-4	0.0	0.0	5.443617200161712	0.0
17	1.431080931204362E-4	0.0	0.0	5.835089388892666	0.0
18	1.431080931204362E-4	0.0	0.0	6.254896980061465	0.0
19	1.431080931204362E-4	0.0	0.0	6.484943239752566	0.0
20	1.431080931204362E-4	0.0	0.0	6.710553148556934	0.0
21	1.431080931204362E-4	0.0	0.0	6.983674944277286	0.0
22	1.431080931204362E-4	0.0	0.0	7.299299843654408	0.0
23	1.431080931204362E-4	0.0	0.0	7.612420351401923	0.0
24	1.431080931204362E-4	0.0	0.0	7.849335799562804	0.0
25	1.431080931204362E-4	0.0	0.0	8.06106422333449	0.0
26	1.431080931204362E-4	0.0	0.0	8.257050756862927	0.0
27	1.431080931204362E-4	0.0	0.0	8.469852491333016	0.0
28	1.431080931204362E-4	0.0	0.0	8.683512874361828	0.0
29	1.431080931204362E-4	0.0	0.0	8.896243054785355	0.0
30	1.431080931204362E-4	0.0	0.0	9.157987757102633	0.0
31	1.431080931204362E-4	0.0	0.0	9.375869828878498	0.0
32	1.431080931204362E-4	0.0	0.0	9.587383590510502	0.0
33	2.146621396806543E-4	0.0	0.0	9.820220458017452	0.0
34	2.146621396806543E-4	0.0	0.0	10.040893137609165	0.0
35	2.146621396806543E-4	0.0	0.0	10.286466625403833	0.0
36	2.146621396806543E-4	0.0	0.0	10.503776264807216	0.0
37	2.146621396806543E-4	0.0	0.0	10.73518205138296	0.0
38	2.146621396806543E-4	0.0	0.0	10.95771513618524	0.0
39	2.146621396806543E-4	0.0	0.0	11.219316730409396	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	40	6.8175723E-9	45.000004	1
TCCGCGA	20	7.0338097E-4	45.000004	29
CGAAACG	25	3.891239E-5	45.000004	1
ACGCATA	20	7.0338097E-4	45.000004	37
CTATACG	25	3.891239E-5	45.000004	1
CGCGATA	20	7.0338097E-4	45.000004	11
AATATCG	40	6.8175723E-9	45.000004	13
TCTACCG	25	3.891239E-5	45.000004	38
CGTAGGC	20	7.0338097E-4	45.000004	33
TTGCGTA	20	7.0338097E-4	45.000004	36
CGTACTG	25	3.891239E-5	45.000004	1
AATTCGC	40	6.8175723E-9	45.000004	37
ACTCGAT	20	7.0338097E-4	45.000004	15
CTCGACG	25	3.891239E-5	45.000004	1
ATCGGCG	20	7.0338097E-4	45.000004	1
CAATACG	80	0.0	45.000004	1
GTGCCGA	40	6.8175723E-9	45.000004	5
CTATCGT	35	1.2121745E-7	45.0	14
CAATTCG	35	1.2121745E-7	45.0	1
TAAGTCG	35	1.2121745E-7	45.0	45
>>END_MODULE