FastQCFastQC Report
Sat 18 Jun 2016
SRR3553325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382706
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245201.7733343169119102No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54020.390683196572518No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43990.3181442765128668No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT33480.2421339026517568No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27790.20098271071362964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC351.2121745E-745.00000445
TAGGTCG351.2121745E-745.0000042
GTTCGTA351.2121745E-745.00000430
GTCACGC351.2121745E-745.00000445
AATTCGC351.2121745E-745.00000437
CGTACGT351.2121745E-745.00000415
TATAGCG351.2121745E-745.0000041
CGCGTAG1400.045.0000041
AACGTCG207.0337835E-445.043
GGTCGTA207.0337835E-445.011
CGAAACG253.8912185E-545.01
CGTCTAT207.0337835E-445.019
CAATTCG406.8175723E-945.01
AATATCG551.8189894E-1245.013
TACGCTC800.045.042
TCGGACG453.8562575E-1045.043
AATTGCG406.8175723E-945.01
TACTAGT253.8912185E-545.025
CACGTTA207.0337835E-445.029
CGGTATG207.0337835E-445.01