##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553325_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1382706
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76983031823106	31.0	31.0	34.0	30.0	34.0
2	31.971267934036593	33.0	31.0	34.0	30.0	34.0
3	32.14427289676909	34.0	31.0	34.0	30.0	34.0
4	35.843874258157555	37.0	35.0	37.0	35.0	37.0
5	35.61850964702547	37.0	35.0	37.0	33.0	37.0
6	32.85054668165178	37.0	35.0	37.0	27.0	37.0
7	34.362646867808486	37.0	35.0	37.0	29.0	37.0
8	35.468712076175265	37.0	35.0	37.0	32.0	37.0
9	37.37652834369707	39.0	37.0	39.0	35.0	39.0
10	36.84241914043911	39.0	37.0	39.0	32.0	39.0
11	36.84761619606771	39.0	37.0	39.0	32.0	39.0
12	36.80435320306703	39.0	37.0	39.0	33.0	39.0
13	36.770831977296695	39.0	37.0	39.0	33.0	39.0
14	37.92781762717454	40.0	37.0	41.0	33.0	41.0
15	37.9704340619047	40.0	37.0	41.0	33.0	41.0
16	37.92109023899513	40.0	37.0	41.0	33.0	41.0
17	37.908599514285754	40.0	37.0	41.0	33.0	41.0
18	37.79212789992956	39.0	37.0	41.0	33.0	41.0
19	37.78842356943559	39.0	36.0	41.0	33.0	41.0
20	37.672898649459825	39.0	36.0	41.0	33.0	41.0
21	37.62425201018872	39.0	36.0	41.0	33.0	41.0
22	37.580423459506214	39.0	36.0	41.0	33.0	41.0
23	37.46546843652953	39.0	36.0	41.0	32.0	41.0
24	37.32540684715334	39.0	35.0	41.0	32.0	41.0
25	37.34498512337402	39.0	35.0	41.0	32.0	41.0
26	37.271229748044775	39.0	35.0	41.0	32.0	41.0
27	37.214779569915805	39.0	35.0	41.0	32.0	41.0
28	37.18168432045569	39.0	35.0	41.0	32.0	41.0
29	36.98018233811092	39.0	35.0	40.0	31.0	41.0
30	37.0153257453139	39.0	35.0	40.0	31.0	41.0
31	36.63343545193266	39.0	35.0	40.0	31.0	41.0
32	36.75801218769572	39.0	35.0	40.0	31.0	41.0
33	36.74518516589933	39.0	35.0	40.0	31.0	41.0
34	36.705447868165756	39.0	35.0	40.0	31.0	41.0
35	36.66271788796751	39.0	35.0	40.0	31.0	41.0
36	36.610557848161505	39.0	35.0	40.0	31.0	41.0
37	36.53124959318901	39.0	35.0	40.0	31.0	41.0
38	36.44341819591439	39.0	35.0	40.0	30.0	41.0
39	36.39501455840938	39.0	35.0	40.0	30.0	41.0
40	36.27166657264813	38.0	35.0	40.0	30.0	41.0
41	36.2178778424336	38.0	35.0	40.0	30.0	41.0
42	36.09754134284512	38.0	35.0	40.0	30.0	41.0
43	36.048218493302265	38.0	35.0	40.0	30.0	41.0
44	35.846777261399026	38.0	35.0	40.0	30.0	41.0
45	35.70719155048145	38.0	34.0	40.0	29.0	41.0
46	35.46356419947552	38.0	34.0	40.0	28.0	41.0
47	35.42120884700001	38.0	34.0	40.0	28.0	41.0
48	35.402078966895346	37.0	34.0	40.0	28.0	41.0
49	35.28319179926897	37.0	34.0	40.0	28.0	41.0
50	35.16619729718393	37.0	34.0	40.0	27.0	41.0
51	34.070177608255115	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	5.0
10	9.0
11	17.0
12	24.0
13	31.0
14	50.0
15	99.0
16	185.0
17	298.0
18	539.0
19	989.0
20	1677.0
21	2546.0
22	3788.0
23	5281.0
24	6752.0
25	7953.0
26	9918.0
27	12179.0
28	15374.0
29	19742.0
30	26031.0
31	34253.0
32	45965.0
33	65004.0
34	97125.0
35	130401.0
36	147470.0
37	199875.0
38	274226.0
39	274854.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.649725972115547	30.187039037944434	20.804567276051454	28.358667713888565
2	21.99838577398232	32.05598297830486	19.76631330159846	26.17931794611436
3	21.495169616679178	32.00600850795469	20.474417555141873	26.024404320224253
4	20.869512390920413	32.79395619893166	19.21109765922763	27.125433750920298
5	18.64243013337615	35.7718849849498	16.571346331034942	29.014338550639106
6	18.153967654729204	43.26429479585682	16.902363915394886	21.67937363401909
7	85.86257671551292	7.371993757168914	3.6706284633175814	3.09480106400059
8	86.84000792648618	7.003947332260076	3.117799445435255	3.038245295818489
9	80.81059892703149	9.98325023540796	5.554687692105191	3.6514631454553608
10	37.23929743560815	34.523029479874964	12.95213877715147	15.285534307365412
11	24.978918150351557	30.937813244464117	24.97761635517601	19.10565225000832
12	26.207017254571834	27.351439857786108	27.823774540647108	18.61776834699495
13	24.676250772036862	28.395045656849682	26.35238438250792	20.57631918860553
14	21.142527768014315	30.4289559747336	27.508884752073108	20.919631505178977
15	19.786418804865242	32.53996149579159	27.103592520752784	20.570027178590387
16	22.122273281521885	30.618511816684098	27.566453027613967	19.69276187418005
17	22.810055065935924	30.224429488264313	27.21243706181936	19.753078383980398
18	21.88874569141958	30.620102899676432	26.69258685505089	20.798564553853097
19	21.4823686307863	32.36053072742868	25.940293887493077	20.216806754291948
20	23.410182641863127	31.776820235104207	26.282955306478744	18.530041816553915
21	23.35333758586424	31.316852606410905	25.89436944657794	19.43544036114691
22	22.735635775067152	29.195722011765334	26.113866577566018	21.954775635601496
23	21.97090343138744	30.764023588528584	25.695194784719238	21.56987819536474
24	20.37078019477749	32.23020656596558	26.42962422959038	20.969389009666553
25	21.54311907231183	32.135898737692614	25.117848624364107	21.20313356563145
26	22.187796972024422	32.03580515308388	24.32447678682236	21.451921088069337
27	20.01285884345624	30.73299747017804	27.033295581273244	22.22084810509248
28	20.746492746831212	31.574463407260833	27.418554631280983	20.26048921462697
29	22.141510921338302	31.97896009708499	24.631194194572092	21.248334787004612
30	22.37901621892145	30.581916907860386	26.526969579939625	20.512097293278543
31	22.68689077793833	31.00637445704293	24.527990765932888	21.77874399908585
32	21.687762980705948	31.082095543087252	25.556047344844096	21.674094131362704
33	21.284712729965737	30.617065377600156	26.912011664084773	21.18621022834934
34	20.499296307385663	30.46012673699254	26.934069860114878	22.106507095506927
35	20.479769379752458	31.063002547179224	28.150163519938438	20.307064553129877
36	22.30965946484647	31.818694646584305	26.04378660394907	19.827859284620157
37	20.76312679629654	32.36689505939802	25.652597153697172	21.21738099060827
38	20.886291084294133	30.977662641226694	27.307468109634296	20.828578164844878
39	20.820261140112216	29.214815007673362	26.595747758381027	23.369176093833396
40	21.96887841666992	30.21061599501268	26.590685221587236	21.229820366730166
41	20.21579424693319	29.297551323274796	28.402567140086177	22.08408728970584
42	21.359204342788708	30.903171028403726	26.52783744338999	21.209787185417582
43	21.083368409481118	30.42353182816882	26.563058235083957	21.930041527266102
44	21.664692277317087	29.927764832147975	25.822119814335082	22.585423076199856
45	22.77302622538703	28.933844215617782	26.77619103410269	21.516938524892492
46	20.155043805407658	30.340506224750598	27.788264461136354	21.716185508705394
47	20.627089200451866	31.224135861130275	26.96704867122874	21.18172626718912
48	20.650015259932335	30.62451453888245	27.212726349636146	21.51274385154906
49	20.963386287468197	30.02048157742861	27.01102041938055	22.00511171572265
50	20.885929474523145	29.56579345139169	27.03040270310536	22.517874370979804
51	20.759510698586684	28.773578765117097	27.993007913468226	22.47390262282799
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28422.0
1	21241.0
2	14060.0
3	8799.5
4	3539.0
5	3221.0
6	2903.0
7	3022.0
8	3141.0
9	3982.0
10	4823.0
11	4970.5
12	5118.0
13	5611.5
14	6105.0
15	6379.5
16	6654.0
17	6473.5
18	6293.0
19	6325.0
20	6357.0
21	6084.5
22	5812.0
23	7892.5
24	9973.0
25	10606.0
26	12296.0
27	13353.0
28	17201.5
29	21050.0
30	26875.0
31	32700.0
32	35024.0
33	37348.0
34	40975.5
35	44603.0
36	46320.5
37	48038.0
38	53594.0
39	59150.0
40	60061.5
41	60973.0
42	68233.0
43	75493.0
44	81012.5
45	86532.0
46	94858.5
47	103185.0
48	103804.0
49	104423.0
50	100610.5
51	96798.0
52	93002.0
53	89206.0
54	81508.5
55	73811.0
56	67671.5
57	61532.0
58	59775.5
59	58019.0
60	54470.5
61	50922.0
62	45740.5
63	40559.0
64	35327.5
65	30096.0
66	27779.0
67	25462.0
68	22003.0
69	18544.0
70	15094.5
71	11645.0
72	10214.0
73	8783.0
74	7782.0
75	5209.5
76	3638.0
77	3077.0
78	2516.0
79	1952.0
80	1388.0
81	988.5
82	589.0
83	489.5
84	390.0
85	390.5
86	391.0
87	303.5
88	216.0
89	133.5
90	51.0
91	49.0
92	47.0
93	35.0
94	23.0
95	16.0
96	9.0
97	5.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1382706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.71813512946771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.71644988164122	15.479855050419447
2	9.72823033970979	4.031015814973901
3	3.6131929724997516	2.245758607592789
4	1.855419846614599	1.537633564162301
5	1.2795108287547534	1.3254539124879103
6	0.9101323999541262	1.1313747628773807
7	0.7036485732205865	1.0204800356548889
8	0.54259215117898	0.8993198006651745
9	0.46278358767684974	0.8629211614669958
>10	4.122616654026577	18.348645545373866
>50	1.098659254241199	16.455430243947678
>100	0.9575697097235705	32.71488609207282
>500	0.007072154429272903	0.9060911273393113
>1k	0.0014144308858545806	0.8490208663272378
>5k	3.5360772146364515E-4	0.3957555198808991
>10k+	3.5360772146364515E-4	1.7963578947574315
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24520	1.7733343169119102	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5402	0.390683196572518	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4399	0.3181442765128668	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	3348	0.2421339026517568	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2779	0.20098271071362964	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2764145089411632	0.0
2	0.0	0.0	0.0	0.38214920597726487	0.0
3	0.0	0.0	0.0	0.4902705275018695	0.0
4	0.0	0.0	0.0	1.0066492804688776	0.0
5	0.0	0.0	0.0	1.0883730887115555	0.0
6	0.0	0.0	0.0	1.5985321536176165	0.0
7	0.0	0.0	0.0	1.973015232449993	0.0
8	0.0	0.0	0.0	2.2534074488719944	0.0
9	0.0	0.0	0.0	2.736735068770946	0.0
10	0.0	0.0	0.0	3.4352928243603484	0.0
11	0.0	0.0	0.0	4.1812937819030225	0.0
12	0.0	0.0	0.0	4.525907893652013	0.0
13	0.0	0.0	0.0	4.665995518931718	0.0
14	0.0	0.0	0.0	4.83175743795138	0.0
15	0.0	0.0	0.0	4.977341531750061	0.0
16	0.0	0.0	0.0	5.264387367958192	0.0
17	0.0	0.0	0.0	5.659988457416111	0.0
18	0.0	0.0	0.0	6.0612306593013985	0.0
19	0.0	0.0	0.0	6.284416209953526	0.0
20	0.0	0.0	0.0	6.500514209094341	0.0
21	0.0	0.0	0.0	6.763549156509049	0.0
22	0.0	0.0	0.0	7.081404145205127	0.0
23	0.0	0.0	0.0	7.384650099153399	0.0
24	0.0	0.0	0.0	7.611307103606985	0.0
25	0.0	0.0	0.0	7.819594331694518	0.0
26	0.0	0.0	0.0	8.006112651568735	0.0
27	0.0	0.0	0.0	8.205214991473241	0.0
28	0.0	0.0	0.0	8.401279809301471	0.0
29	0.0	0.0	0.0	8.609060783709625	0.0
30	0.0	0.0	0.0	8.85857152568948	0.0
31	0.0	0.0	0.0	9.074163271150917	0.0
32	0.0	0.0	0.0	9.28317371878042	0.0
33	0.0	0.0	0.0	9.509686079325613	0.0
34	0.0	0.0	0.0	9.724409961336683	0.0
35	0.0	0.0	0.0	9.957720585576398	0.0
36	0.0	0.0	0.0	10.170130165053164	0.0
37	0.0	0.0	0.0	10.392013920529743	0.0
38	0.0	0.0	0.0	10.617875383487162	0.0
39	0.0	0.0	0.0	10.874329033069937	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	35	1.2121745E-7	45.000004	45
TAGGTCG	35	1.2121745E-7	45.000004	2
GTTCGTA	35	1.2121745E-7	45.000004	30
GTCACGC	35	1.2121745E-7	45.000004	45
AATTCGC	35	1.2121745E-7	45.000004	37
CGTACGT	35	1.2121745E-7	45.000004	15
TATAGCG	35	1.2121745E-7	45.000004	1
CGCGTAG	140	0.0	45.000004	1
AACGTCG	20	7.0337835E-4	45.0	43
GGTCGTA	20	7.0337835E-4	45.0	11
CGAAACG	25	3.8912185E-5	45.0	1
CGTCTAT	20	7.0337835E-4	45.0	19
CAATTCG	40	6.8175723E-9	45.0	1
AATATCG	55	1.8189894E-12	45.0	13
TACGCTC	80	0.0	45.0	42
TCGGACG	45	3.8562575E-10	45.0	43
AATTGCG	40	6.8175723E-9	45.0	1
TACTAGT	25	3.8912185E-5	45.0	25
CACGTTA	20	7.0337835E-4	45.0	29
CGGTATG	20	7.0337835E-4	45.0	1
>>END_MODULE