SRR3553324_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553324_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1249206
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22562	1.806107239318415	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5049	0.40417673306084023	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4308	0.34485905447140025	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	3657	0.2927459522288558	TruSeq Adapter, Index 20 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2485	0.19892635802261593	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1682	0.13464552683864792	No Hit
ACGAATGGGGACATTGTTGGGGTTTTGAGTTTGTTTTCTTCTGCTTTGATT	1610	0.12888186576113148	No Hit
CAAGTGGGGGCAATGTTTTCTGTTTTGTTTTTGTTTCTGTTTTTAGTTTGA	1510	0.12087678093124753	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.033522E-4	45.0	11
ATCTCCG	40	6.8157533E-9	45.0	1
CGAACTA	40	6.8157533E-9	45.0	39
CGAACAG	80	0.0	45.0	1
TCCGCGT	20	7.033522E-4	45.0	38
CGTATAG	35	1.2120472E-7	45.0	1
GTCGAGT	25	3.8910017E-5	45.0	14
GTCGAAC	40	6.8157533E-9	45.0	37
ATAGCGA	25	3.8910017E-5	45.0	14
CACGAAC	50	2.1827873E-11	45.0	35
CGCGACA	20	7.033522E-4	45.0	20
TCGATAG	25	3.8910017E-5	45.0	1
TCTAGCG	55	1.8189894E-12	45.0	1
CGTTACA	20	7.033522E-4	45.0	24
AATGCGA	20	7.033522E-4	45.0	44
GTTCGCA	20	7.033522E-4	45.0	35
CGTAGAT	50	2.1827873E-11	45.0	14
TACGACT	50	2.1827873E-11	45.0	20
TGCGACG	55	1.8189894E-12	45.0	1
AACGCAT	25	3.8910017E-5	45.0	41