##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553324_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1249206
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6839912712555	31.0	31.0	34.0	30.0	34.0
2	31.883602064031074	33.0	31.0	34.0	30.0	34.0
3	32.04587874217703	33.0	31.0	34.0	30.0	34.0
4	35.77222411675896	37.0	35.0	37.0	35.0	37.0
5	35.52904004623737	37.0	35.0	37.0	33.0	37.0
6	34.164842307833936	37.0	35.0	37.0	32.0	37.0
7	35.02455079466477	37.0	35.0	37.0	32.0	37.0
8	35.72206905826581	37.0	35.0	37.0	33.0	37.0
9	37.44060627310468	39.0	37.0	39.0	35.0	39.0
10	36.81406749567325	39.0	37.0	39.0	32.0	39.0
11	36.816501041461535	39.0	37.0	39.0	32.0	39.0
12	36.794296537160406	39.0	37.0	39.0	32.0	39.0
13	36.765880887539765	39.0	37.0	39.0	33.0	39.0
14	37.921832748161634	40.0	37.0	41.0	33.0	41.0
15	37.92507240599229	40.0	37.0	41.0	33.0	41.0
16	37.836459318959406	40.0	37.0	41.0	33.0	41.0
17	37.799422993485464	39.0	37.0	41.0	33.0	41.0
18	37.71364210546539	39.0	37.0	41.0	33.0	41.0
19	37.699385849891854	39.0	36.0	41.0	33.0	41.0
20	37.60790774299835	39.0	36.0	41.0	33.0	41.0
21	37.57949049236075	39.0	36.0	41.0	32.0	41.0
22	37.55940733553953	39.0	36.0	41.0	32.0	41.0
23	37.36528322790637	39.0	36.0	41.0	32.0	41.0
24	37.19571551849735	39.0	35.0	41.0	32.0	41.0
25	37.25696082151383	39.0	35.0	41.0	32.0	41.0
26	37.16736951311473	39.0	35.0	41.0	32.0	41.0
27	37.1338994529325	39.0	35.0	41.0	32.0	41.0
28	37.042858423670715	39.0	35.0	40.0	31.0	41.0
29	36.89395984329246	39.0	35.0	40.0	31.0	41.0
30	36.92316479427733	39.0	35.0	40.0	31.0	41.0
31	36.843346093438555	39.0	35.0	40.0	31.0	41.0
32	36.771800647771464	39.0	35.0	40.0	31.0	41.0
33	36.692941756603794	39.0	35.0	40.0	31.0	41.0
34	36.614476715609754	39.0	35.0	40.0	31.0	41.0
35	36.50319082681319	39.0	35.0	40.0	30.0	41.0
36	36.439684087332274	39.0	35.0	40.0	30.0	41.0
37	36.37052415694449	38.0	35.0	40.0	30.0	41.0
38	36.26786694908606	38.0	35.0	40.0	30.0	41.0
39	36.21733405058893	38.0	35.0	40.0	30.0	41.0
40	36.085810506833944	38.0	35.0	40.0	30.0	41.0
41	35.97541958652136	38.0	35.0	40.0	30.0	41.0
42	35.87270634306912	38.0	35.0	40.0	29.0	41.0
43	35.79386346207111	38.0	35.0	40.0	29.0	41.0
44	35.66557397258739	38.0	35.0	40.0	29.0	41.0
45	35.492990747722956	38.0	34.0	40.0	28.0	41.0
46	35.3609100500638	38.0	34.0	40.0	27.0	41.0
47	35.25424469623105	37.0	34.0	40.0	27.0	41.0
48	35.09223378690144	37.0	34.0	40.0	27.0	41.0
49	34.94662609689675	37.0	34.0	40.0	27.0	41.0
50	34.86471406637496	37.0	34.0	40.0	26.0	41.0
51	33.69891515090385	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	11.0
10	7.0
11	13.0
12	18.0
13	15.0
14	31.0
15	72.0
16	144.0
17	279.0
18	570.0
19	1111.0
20	1660.0
21	2623.0
22	3648.0
23	5058.0
24	6603.0
25	7864.0
26	9681.0
27	12139.0
28	14961.0
29	19524.0
30	25036.0
31	32632.0
32	42968.0
33	57952.0
34	84592.0
35	115347.0
36	130808.0
37	175339.0
38	247380.0
39	251068.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.602526724975704	32.34662657720184	21.021512864971832	26.029333832850625
2	23.75901172424724	32.02922496369694	19.784567157058163	24.427196154997656
3	20.96715833897692	33.08157341543348	19.355974915266177	26.595293330323422
4	20.576270046733686	34.03641993394204	18.08308637646633	27.304223642857945
5	17.941476425825684	36.12118417618871	16.194927017641607	29.742412380343996
6	18.32964298922676	41.74947926923181	17.55274950648652	22.368128235054908
7	85.12327030129538	8.001082287469	3.559060715366401	3.316586695869216
8	86.55521987566502	7.1863247534834125	3.274479949664027	2.9839754211875387
9	79.74009090574333	10.894920453472047	5.547683888806169	3.8173047519784564
10	32.364958221462274	41.31424280703103	12.349284265365359	13.971514706141342
11	23.30600397372411	30.548764575258204	27.270121981482635	18.875109469535047
12	24.308640848667075	26.688952822833063	30.129378181020584	18.87302814747928
13	24.73291034465092	27.097772505095236	27.858255563934208	20.31106158631963
14	19.997662515229674	31.36936582116961	28.041812159083452	20.59115950451727
15	19.63183013850398	32.52409930788037	27.943749869917372	19.900320683698286
16	21.188819137916404	31.187250141289745	27.573674798231835	20.05025592256201
17	19.93450239592189	30.085350214456223	28.422934247834224	21.557213141787663
18	20.450910418297703	30.429168607899737	28.23889734759519	20.88102362620737
19	20.80489526947517	33.20517192520689	26.57552077079361	19.414412034524332
20	23.377489381254975	31.62088558652456	25.707289270144397	19.29433576207607
21	23.230435972930007	30.757216984228382	26.270847242168227	19.741499800673388
22	22.203303538407596	28.4727258754761	26.20848763134343	23.115482954772872
23	20.722602997423962	32.24520215240721	25.69007833776015	21.342116512408683
24	19.22068898164114	32.416911222008224	27.49882725507242	20.86357254127822
25	21.436336360856416	31.687968197398988	25.489550962771553	21.386144478973044
26	22.235804182816928	31.965744640995965	25.018211567987986	20.78023960819913
27	19.533367595096404	31.059168784011604	26.603218364305008	22.804245256586984
28	19.500546747293882	32.090303760948956	28.734572200261603	19.674577291495556
29	20.547531792194402	32.34126317036581	25.839613322382377	21.271591715057404
30	21.41120039449058	28.79757221787279	28.639631894179185	21.151595493457446
31	21.727561346967594	31.59887160324238	24.433520172013264	22.240046877776763
32	22.12845599524818	30.161718723733316	27.191592099301477	20.518233181717026
33	20.067626956642858	30.74576971292165	26.407333938517745	22.779269391917747
34	20.06122288877895	30.575181355196822	28.13843353298015	21.225162223044077
35	20.351247112165645	28.97040199934999	29.081352475092174	21.59699841339219
36	21.610126752513196	30.74504925528696	28.229691500040826	19.41513249215902
37	20.499020978125305	32.14017543943913	26.72033275536621	20.640470827069354
38	21.106046560775404	31.314691091781498	26.8589007737715	20.720361573671596
39	20.682977827516037	28.768673861636913	26.785334044184868	23.763014266662182
40	21.67833007526381	28.831433726703203	29.107288949941	20.382947248091988
41	20.049855668320518	29.03364216950607	29.370816342540785	21.54568581963263
42	21.37461715681801	30.85095652758632	26.630355601878314	21.14407071371735
43	20.699788505658795	29.11313266186682	27.48369764474394	22.703381187730447
44	21.666482549715578	28.984891202892076	27.231377370906003	22.117248876486347
45	22.8100089176645	28.998900101344372	27.843446156998926	20.3476448239922
46	20.605968911452553	28.34104223002451	29.643549582694927	21.409439275828007
47	20.875420066826447	30.464951337089317	27.97096715833898	20.688661437745257
48	20.314263620251584	29.755540719465007	28.69983013209991	21.230365528183505
49	21.22772385018964	29.99265133212617	27.469128390353553	21.31049642733064
50	20.348605434171784	28.94534608383245	27.952395361533643	22.75365312046212
51	20.855567456448338	28.543650927068875	28.53164329982405	22.069138316658744
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26029.0
1	19604.5
2	13180.0
3	8141.5
4	3103.0
5	2833.0
6	2563.0
7	2766.0
8	2969.0
9	3755.0
10	4541.0
11	5283.0
12	6025.0
13	6167.5
14	6310.0
15	6506.5
16	6703.0
17	6805.5
18	6908.0
19	7075.5
20	7243.0
21	7384.5
22	7526.0
23	9848.5
24	12171.0
25	12432.0
26	14516.5
27	16340.0
28	19645.0
29	22950.0
30	24012.5
31	25075.0
32	31228.0
33	37381.0
34	38133.5
35	38886.0
36	43151.5
37	47417.0
38	47855.5
39	48294.0
40	53139.5
41	57985.0
42	61508.5
43	65032.0
44	72338.5
45	79645.0
46	89326.5
47	99008.0
48	96692.0
49	94376.0
50	92795.0
51	91214.0
52	83014.0
53	74814.0
54	67155.5
55	59497.0
56	56529.0
57	53561.0
58	49729.0
59	45897.0
60	42608.0
61	39319.0
62	36591.0
63	33863.0
64	31180.0
65	28497.0
66	24474.0
67	20451.0
68	18126.5
69	15802.0
70	13259.5
71	10717.0
72	10165.0
73	9613.0
74	8150.0
75	5151.0
76	3615.0
77	2916.0
78	2217.0
79	1804.5
80	1392.0
81	1101.0
82	810.0
83	574.5
84	339.0
85	386.5
86	434.0
87	248.5
88	63.0
89	43.0
90	23.0
91	19.0
92	15.0
93	12.5
94	10.0
95	6.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1249206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.997995758636637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.13905225698241	16.529085528570757
2	10.104755332972713	4.445687299135894
3	3.9004091084518646	2.574035490740155
4	1.9260717166270809	1.6947886981277007
5	1.228218900185334	1.350917707847717
6	0.8561550371913149	1.1300216926121964
7	0.6409862416850588	0.9870288838152653
8	0.49082574431476556	0.8637746113332708
9	0.383874487839044	0.7600022419798892
>10	3.5209855892134754	16.773804656243854
>50	0.9009820373009484	14.385570460733138
>100	0.8927912823100916	33.30659863549181
>500	0.011169198694454851	1.5176888104828399
>1k	0.00297845298518796	1.4196545162651528
>5k	3.72306623148495E-4	0.41351307560994327
>10k+	3.72306623148495E-4	1.847827691010406
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22562	1.806107239318415	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5049	0.40417673306084023	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4308	0.34485905447140025	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	3657	0.2927459522288558	TruSeq Adapter, Index 20 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2485	0.19892635802261593	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1682	0.13464552683864792	No Hit
ACGAATGGGGACATTGTTGGGGTTTTGAGTTTGTTTTCTTCTGCTTTGATT	1610	0.12888186576113148	No Hit
CAAGTGGGGGCAATGTTTTCTGTTTTGTTTTTGTTTCTGTTTTTAGTTTGA	1510	0.12087678093124753	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.005084829883942E-5	0.0	0.0	0.3516633765768016	0.0
2	8.005084829883942E-5	0.0	0.0	0.4911920051616787	0.0
3	8.005084829883942E-5	0.0	0.0	0.6190332098949253	0.0
4	8.005084829883942E-5	0.0	0.0	1.2468720131027229	0.0
5	8.005084829883942E-5	0.0	0.0	1.3514184209810072	0.0
6	8.005084829883942E-5	0.0	0.0	2.005593953279123	0.0
7	8.005084829883942E-5	0.0	0.0	2.4676474496600243	0.0
8	8.005084829883942E-5	0.0	0.0	2.851891521494453	0.0
9	8.005084829883942E-5	0.0	0.0	3.4392245954630383	0.0
10	8.005084829883942E-5	0.0	0.0	4.458752199397057	0.0
11	8.005084829883942E-5	0.0	0.0	5.408875717855982	0.0
12	8.005084829883942E-5	0.0	0.0	5.899507367079569	0.0
13	8.005084829883942E-5	0.0	0.0	6.0985938267987825	0.0
14	8.005084829883942E-5	0.0	0.0	6.309367710369627	0.0
15	8.005084829883942E-5	0.0	0.0	6.492123797035878	0.0
16	8.005084829883942E-5	0.0	0.0	6.88093076722334	0.0
17	8.005084829883942E-5	0.0	0.0	7.384450603023041	0.0
18	8.005084829883942E-5	0.0	0.0	8.021975558874997	0.0
19	8.005084829883942E-5	0.0	0.0	8.305435612701189	0.0
20	8.005084829883942E-5	0.0	0.0	8.618034175308155	0.0
21	8.005084829883942E-5	0.0	0.0	8.951045704231328	0.0
22	8.005084829883942E-5	0.0	0.0	9.33024657262293	0.0
23	8.005084829883942E-5	0.0	0.0	9.707846424048556	0.0
24	8.005084829883942E-5	0.0	0.0	9.999151461008033	0.0
25	1.6010169659767885E-4	0.0	0.0	10.236021921124298	0.0
26	1.6010169659767885E-4	0.0	0.0	10.461685262478726	0.0
27	1.6010169659767885E-4	0.0	0.0	10.704479485369106	0.0
28	1.6010169659767885E-4	0.0	0.0	10.957920471083233	0.0
29	1.6010169659767885E-4	0.0	0.0	11.217845575509564	0.0
30	1.6010169659767885E-4	0.0	0.0	11.56302483337416	0.0
31	1.6010169659767885E-4	0.0	0.0	11.821348920834515	0.0
32	1.6010169659767885E-4	0.0	0.0	12.08239473713703	0.0
33	1.6010169659767885E-4	0.0	0.0	12.360171180734003	0.0
34	1.6010169659767885E-4	0.0	0.0	12.617294505469875	0.0
35	1.6010169659767885E-4	0.0	0.0	12.92388925445443	0.0
36	1.6010169659767885E-4	0.0	0.0	13.199824528540528	0.0
37	1.6010169659767885E-4	0.0	0.0	13.468235022886537	0.0
38	1.6010169659767885E-4	0.0	0.0	13.75009405974675	0.0
39	1.6010169659767885E-4	0.0	0.0	14.035475333932114	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.033522E-4	45.0	11
ATCTCCG	40	6.8157533E-9	45.0	1
CGAACTA	40	6.8157533E-9	45.0	39
CGAACAG	80	0.0	45.0	1
TCCGCGT	20	7.033522E-4	45.0	38
CGTATAG	35	1.2120472E-7	45.0	1
GTCGAGT	25	3.8910017E-5	45.0	14
GTCGAAC	40	6.8157533E-9	45.0	37
ATAGCGA	25	3.8910017E-5	45.0	14
CACGAAC	50	2.1827873E-11	45.0	35
CGCGACA	20	7.033522E-4	45.0	20
TCGATAG	25	3.8910017E-5	45.0	1
TCTAGCG	55	1.8189894E-12	45.0	1
CGTTACA	20	7.033522E-4	45.0	24
AATGCGA	20	7.033522E-4	45.0	44
GTTCGCA	20	7.033522E-4	45.0	35
CGTAGAT	50	2.1827873E-11	45.0	14
TACGACT	50	2.1827873E-11	45.0	20
TGCGACG	55	1.8189894E-12	45.0	1
AACGCAT	25	3.8910017E-5	45.0	41
>>END_MODULE