FastQCFastQC Report
Sat 18 Jun 2016
SRR3553320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308289
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169601.2963496597464323No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39280.30023947308278215No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34370.26270953894743443No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT23240.1776365925265748Illumina Single End Adapter 2 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19340.14782666520929244No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG18080.13819576561447813No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGT17250.1318516015956719No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC14330.10953237396324512No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT13220.10104801003448015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT700.045.00000442
ATCACCG650.045.0000041
ACGTAAG351.2121018E-745.0000041
GTCGATA406.8157533E-945.031
ACCGCGT207.0336444E-445.025
CGCATAT253.891102E-545.016
TAACGTA207.0336444E-445.045
TCGATAT406.8157533E-945.032
CGCTATA207.0336444E-445.037
CGTAGCG406.8157533E-945.01
AGCGATC207.0336444E-445.024
GAACGTT502.1827873E-1145.038
GGACGTA253.891102E-545.09
CTCGCTA253.891102E-545.01
GCGATAC253.891102E-545.029
TCGTACT406.8157533E-945.029
TATAGCG253.891102E-545.01
CCGCGTA207.0336444E-445.018
TGTGTCG253.891102E-545.011
TATCGCG800.045.01