##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553320_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1308289
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.758521244159358	31.0	31.0	34.0	30.0	34.0
2	31.960390250166437	33.0	31.0	34.0	30.0	34.0
3	32.11148836380953	34.0	31.0	34.0	30.0	34.0
4	35.820223971920576	37.0	35.0	37.0	35.0	37.0
5	35.55855090121525	37.0	35.0	37.0	33.0	37.0
6	34.23872248409946	37.0	35.0	37.0	32.0	37.0
7	35.007465475900204	37.0	35.0	37.0	32.0	37.0
8	35.69745828329979	37.0	35.0	37.0	33.0	37.0
9	37.41344534732005	39.0	37.0	39.0	35.0	39.0
10	36.82340522621531	39.0	37.0	39.0	32.0	39.0
11	36.80594654544982	39.0	37.0	39.0	32.0	39.0
12	36.721082268520185	39.0	35.0	39.0	32.0	39.0
13	36.68228732336662	39.0	35.0	39.0	32.0	39.0
14	37.87344768625281	40.0	37.0	41.0	33.0	41.0
15	37.86904269622384	40.0	37.0	41.0	33.0	41.0
16	37.791491023772274	40.0	37.0	41.0	33.0	41.0
17	37.75468875760631	39.0	37.0	41.0	33.0	41.0
18	37.679469903056585	39.0	36.0	41.0	33.0	41.0
19	37.68211534301672	39.0	36.0	41.0	33.0	41.0
20	37.57663482609729	39.0	36.0	41.0	32.0	41.0
21	37.54765881238778	39.0	36.0	41.0	32.0	41.0
22	37.53452027801197	39.0	36.0	41.0	32.0	41.0
23	37.38202644828475	39.0	36.0	41.0	32.0	41.0
24	37.18210425983862	39.0	35.0	41.0	32.0	41.0
25	37.24895875452595	39.0	35.0	41.0	32.0	41.0
26	37.15282861814171	39.0	35.0	41.0	32.0	41.0
27	37.10198281878087	39.0	35.0	41.0	31.0	41.0
28	37.03503583688313	39.0	35.0	40.0	31.0	41.0
29	36.858638267233005	39.0	35.0	40.0	31.0	41.0
30	36.88275526278979	39.0	35.0	40.0	31.0	41.0
31	36.820143714423956	39.0	35.0	40.0	31.0	41.0
32	36.74434318411299	39.0	35.0	40.0	31.0	41.0
33	36.66853883201647	39.0	35.0	40.0	31.0	41.0
34	36.58924366099539	39.0	35.0	40.0	31.0	41.0
35	36.448064609577855	39.0	35.0	40.0	30.0	41.0
36	36.40030681294424	38.0	35.0	40.0	30.0	41.0
37	36.33709447988938	38.0	35.0	40.0	30.0	41.0
38	36.222553273779724	38.0	35.0	40.0	30.0	41.0
39	36.176241640799546	38.0	35.0	40.0	30.0	41.0
40	36.02443267504351	38.0	35.0	40.0	30.0	41.0
41	35.91664456400688	38.0	35.0	40.0	29.0	41.0
42	35.80473351071514	38.0	35.0	40.0	29.0	41.0
43	35.72196662969726	38.0	34.0	40.0	29.0	41.0
44	35.62222414160786	38.0	34.0	40.0	29.0	41.0
45	35.462536182754725	38.0	34.0	40.0	28.0	41.0
46	35.29239793348412	37.0	34.0	40.0	27.0	41.0
47	35.1759595930257	37.0	34.0	40.0	27.0	41.0
48	35.03935216148726	37.0	34.0	40.0	27.0	41.0
49	34.86350034281416	37.0	34.0	40.0	26.0	41.0
50	34.76707057844253	37.0	33.0	40.0	26.0	41.0
51	33.56738534070072	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	13.0
10	19.0
11	29.0
12	27.0
13	53.0
14	71.0
15	94.0
16	184.0
17	342.0
18	624.0
19	1114.0
20	1843.0
21	2735.0
22	3764.0
23	5200.0
24	6749.0
25	8287.0
26	10358.0
27	12800.0
28	15904.0
29	20861.0
30	27410.0
31	35039.0
32	45557.0
33	61699.0
34	92373.0
35	117053.0
36	133822.0
37	184924.0
38	258990.0
39	260313.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.673184594535304	31.833257025015115	20.29834386744825	27.195214513001332
2	23.884860302272664	30.81398681789727	19.304144573561345	25.99700830626872
3	21.128282818245818	31.80765106180668	20.449533703944617	26.614532416002884
4	21.71271026508669	31.740234764642985	18.38959129060934	28.157463679660992
5	18.393948126140323	36.09378356005439	16.409447759631092	29.10282055417419
6	19.168241879278966	41.16208268968095	17.01734096977044	22.652334461269643
7	85.02563271570732	7.649685963881068	3.8388307170663363	3.4858506033452854
8	86.78296614891664	6.447122921617471	3.2041085723414326	3.5658023571244577
9	80.30725627135901	9.690672320871	5.498632182950403	4.5034392248195925
10	36.52136492777972	34.09376674419796	13.037639237202178	16.347229090820147
11	26.92707803856793	31.902584214955564	22.560229429430347	18.610108317046155
12	26.837571820905016	26.716344783148067	27.76282610340682	18.683257292540105
13	24.401336401972348	27.08583501046023	26.956811530174146	21.55601705739328
14	21.519175044657565	28.570292955149817	26.974238872298095	22.936293127894526
15	20.5638050919942	30.736175264028056	26.835660928128263	21.86435871584948
16	22.582625092773846	28.806861480911326	26.719249340168723	21.891264086146105
17	21.808484211057344	29.343975222599898	26.854311241629336	21.993229324713422
18	22.849920774385478	29.187740629172914	25.774274644210877	22.18806395223074
19	22.77386724187087	30.62893596139691	25.289519364605223	21.307677432127
20	24.275905400106552	29.489508816477095	26.295489757996894	19.93909602541946
21	25.149871320480415	28.213491055875267	26.980506600605832	19.656131023038487
22	24.19473067495026	27.320951257711407	24.88509801733409	23.59922005000424
23	21.76354001294821	29.44288303272442	26.01940396961222	22.77417298471515
24	21.399553156833086	30.969151311369274	26.29908223641718	21.332213295380456
25	23.1643008540162	29.265552183042125	24.867364932365863	22.702782030575815
26	23.63147592007576	30.589265827351603	23.394295908625693	22.384962343946942
27	21.74756494933459	30.355831165743965	25.047447467646673	22.849156417274777
28	22.091525649149386	29.822768516742098	27.086752238993068	20.998953595115452
29	22.38435085825838	29.988022524075337	25.918279523866666	21.70934709379961
30	22.70362282339758	28.41321756890106	26.82304903580172	22.06011057189963
31	23.81935489788571	29.007428786758886	23.932861928824593	23.240354386530804
32	22.750477914283465	28.92036851185021	25.59870181588319	22.730451757983136
33	22.52239375245072	27.886728391051214	25.389497274684718	24.201380581813346
34	22.024185787696755	28.15937457243774	26.918517238928096	22.897922400937407
35	21.768431898456686	28.56517176250813	26.983640464759695	22.682755874275486
36	24.322989798125644	30.3753222720668	24.527684632370985	20.774003297436575
37	21.797401032952198	30.53835964377901	24.020686560843973	23.643552762424815
38	21.940947298341573	29.861215679410286	25.447970593653235	22.749866428594906
39	21.197610008186267	28.991453723145266	25.25672844455621	24.554207824112257
40	22.92597430690008	28.56264938404282	25.943656179941893	22.567720129115205
41	21.85961970176314	26.835431620995053	28.24498256883609	23.05996610840571
42	22.306615740100238	28.74907608334244	25.514316790861958	23.429991385695363
43	22.465984197681095	28.36697396370374	26.278521030139366	22.888520808475803
44	23.48341994773326	26.295795500841173	26.145293585744433	24.07549096568113
45	23.53272098137338	27.516397370917282	26.496821420955158	22.45406022675418
46	22.04704006530667	28.168011807788645	27.53558273439584	22.249365392508842
47	22.223835865011477	30.06751566358809	25.655340677786025	22.053307793614408
48	21.10382339070343	29.249194940873156	27.223495726097212	22.4234859423262
49	21.87964585806347	29.221754520599042	26.404869260538	22.49373036079949
50	21.402763456698022	28.100289767780666	27.170907956881084	23.326038818640225
51	22.227046164876416	27.26026130312186	27.099211259897473	23.413481272104253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19773.0
1	15101.0
2	10429.0
3	6630.0
4	2831.0
5	2388.0
6	1945.0
7	2044.5
8	2144.0
9	2599.0
10	3054.0
11	3467.5
12	3881.0
13	3693.0
14	3505.0
15	3472.5
16	3440.0
17	3824.0
18	4208.0
19	4738.0
20	5268.0
21	5981.0
22	6694.0
23	7224.5
24	7755.0
25	8569.5
26	12037.5
27	14691.0
28	18640.5
29	22590.0
30	24116.5
31	25643.0
32	28978.0
33	32313.0
34	35787.0
35	39261.0
36	45173.0
37	51085.0
38	51675.5
39	52266.0
40	52890.5
41	53515.0
42	57533.5
43	61552.0
44	64353.5
45	67155.0
46	73315.0
47	79475.0
48	81700.5
49	83926.0
50	91805.5
51	99685.0
52	93413.5
53	87142.0
54	81928.0
55	76714.0
56	71865.5
57	67017.0
58	63527.0
59	60037.0
60	57963.0
61	55889.0
62	51831.5
63	47774.0
64	43486.5
65	39199.0
66	34620.5
67	30042.0
68	26199.0
69	22356.0
70	20173.5
71	17991.0
72	15819.0
73	13647.0
74	11592.0
75	7600.5
76	5664.0
77	4681.5
78	3699.0
79	2935.5
80	2172.0
81	1487.0
82	802.0
83	706.0
84	610.0
85	444.0
86	278.0
87	195.0
88	112.0
89	103.5
90	95.0
91	57.5
92	20.0
93	18.5
94	17.0
95	11.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1308289.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.689908841816305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.09019834645882	14.408087796928534
2	9.180618578171133	3.431698486750074
3	3.323795981707049	1.8636433172070026
4	1.6603477541545115	1.24126992683449
5	1.1121600356226409	1.0393084841649165
6	0.7178835862204704	0.8050307271298064
7	0.5284967950672104	0.6914289846098766
8	0.40894176313266783	0.6114467419648957
9	0.29486153021805284	0.4959841608660485
>10	3.121774268175238	13.46574602733516
>50	1.2139732055012533	16.739876528968885
>100	1.3347832547576532	41.45251536748459
>500	0.007550628091023807	0.8555041385851577
>1k	0.004194793383902115	1.5687901221672476
>5k	0.0	0.0
>10k+	4.194793383902115E-4	1.3296691890033243
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16960	1.2963496597464323	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3928	0.30023947308278215	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3437	0.26270953894743443	No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	2324	0.1776365925265748	Illumina Single End Adapter 2 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1934	0.14782666520929244	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1808	0.13819576561447813	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGT	1725	0.1318516015956719	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC	1433	0.10953237396324512	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	1322	0.10104801003448015	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5287142213990946E-4	0.0	0.0	0.21677167659439162	0.0
2	1.5287142213990946E-4	0.0	0.0	0.29290164482006653	0.0
3	1.5287142213990946E-4	0.0	0.0	0.3843951909708023	0.0
4	1.5287142213990946E-4	0.0	0.0	0.8263464723772805	0.0
5	1.5287142213990946E-4	0.0	0.0	0.899801190715507	0.0
6	1.5287142213990946E-4	0.0	0.0	1.3189746302231389	0.0
7	1.5287142213990946E-4	0.0	0.0	1.610576867955016	0.0
8	1.5287142213990946E-4	0.0	0.0	1.8086982310483386	0.0
9	1.5287142213990946E-4	0.0	0.0	2.250420205321607	0.0
10	1.5287142213990946E-4	0.0	0.0	2.745188563077424	0.0
11	1.5287142213990946E-4	0.0	0.0	3.3589673229691606	0.0
12	1.5287142213990946E-4	0.0	0.0	3.594312877353551	0.0
13	2.293071332098642E-4	0.0	0.0	3.699106237230459	0.0
14	2.293071332098642E-4	0.0	0.0	3.8262952604508635	0.0
15	2.293071332098642E-4	0.0	0.0	3.9328466416823806	0.0
16	2.293071332098642E-4	0.0	0.0	4.148930396877143	0.0
17	2.293071332098642E-4	0.0	0.0	4.416302514199844	0.0
18	2.293071332098642E-4	0.0	0.0	4.748186371665588	0.0
19	2.293071332098642E-4	0.0	0.0	4.89708313682986	0.0
20	2.293071332098642E-4	0.0	0.0	5.061343479919192	0.0
21	2.293071332098642E-4	0.0	0.0	5.2593884073014445	0.0
22	2.293071332098642E-4	0.0	0.0	5.478223848094725	0.0
23	2.293071332098642E-4	0.0	0.0	5.714410195300886	0.0
24	2.293071332098642E-4	0.0	0.0	5.890135895050712	0.0
25	2.293071332098642E-4	0.0	0.0	6.0465233598998385	0.0
26	2.293071332098642E-4	0.0	0.0	6.178757140050861	0.0
27	2.293071332098642E-4	0.0	0.0	6.328494698036901	0.0
28	2.293071332098642E-4	0.0	0.0	6.481136813043602	0.0
29	2.293071332098642E-4	0.0	0.0	6.646237948954703	0.0
30	2.293071332098642E-4	0.0	0.0	6.839620297961689	0.0
31	2.293071332098642E-4	0.0	0.0	7.012365004979786	0.0
32	2.293071332098642E-4	0.0	0.0	7.17509663384772	0.0
33	2.293071332098642E-4	0.0	0.0	7.346159755222279	0.0
34	2.293071332098642E-4	0.0	0.0	7.501094941561077	0.0
35	3.057428442798189E-4	0.0	0.0	7.688821047948886	0.0
36	3.057428442798189E-4	0.0	0.0	7.857208919435996	0.0
37	3.057428442798189E-4	0.0	0.0	8.007863705954877	0.0
38	3.057428442798189E-4	0.0	0.0	8.185270991348242	0.0
39	3.057428442798189E-4	0.0	0.0	8.39065374699321	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATT	70	0.0	45.000004	42
ATCACCG	65	0.0	45.000004	1
ACGTAAG	35	1.2121018E-7	45.000004	1
GTCGATA	40	6.8157533E-9	45.0	31
ACCGCGT	20	7.0336444E-4	45.0	25
CGCATAT	25	3.891102E-5	45.0	16
TAACGTA	20	7.0336444E-4	45.0	45
TCGATAT	40	6.8157533E-9	45.0	32
CGCTATA	20	7.0336444E-4	45.0	37
CGTAGCG	40	6.8157533E-9	45.0	1
AGCGATC	20	7.0336444E-4	45.0	24
GAACGTT	50	2.1827873E-11	45.0	38
GGACGTA	25	3.891102E-5	45.0	9
CTCGCTA	25	3.891102E-5	45.0	1
GCGATAC	25	3.891102E-5	45.0	29
TCGTACT	40	6.8157533E-9	45.0	29
TATAGCG	25	3.891102E-5	45.0	1
CCGCGTA	20	7.0336444E-4	45.0	18
TGTGTCG	25	3.891102E-5	45.0	11
TATCGCG	80	0.0	45.0	1
>>END_MODULE