FastQCFastQC Report
Sat 18 Jun 2016
SRR3553318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604819
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA213623.5319657616576197No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44710.7392294223561098No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42180.6973987258998147No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT32960.5449564249800354TruSeq Adapter, Index 10 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18780.3105061183593769No Hit
CAGAGAGAGGGAAAAGGATCGTGAAAAAGACAAGGAGCGGGGTAAAAACAA10760.1779044639801329No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9930.16418135012292934No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA9920.16401601140175823No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC9400.1554183979008596No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGT8730.14434070358239406No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8470.14004189683194476No Hit
AATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT6640.10978491085762848RNA PCR Primer, Index 35 (95% over 22bp)
AGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6280.1038327168954679No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG253.8886054E-545.0000041
GATAATC302.1637898E-645.0000049
CATATCG253.8886054E-545.00000432
CGAGTTT253.8886054E-545.00000437
GATCGCG302.1637898E-645.0000041
ACGCATG302.1637898E-645.0000041
CGACGAA253.8886054E-545.00000439
GCGCGTA302.1637898E-645.00000434
TAGGTGT302.1637898E-645.00000420
TAGAACG253.8886054E-545.0000041
GACCGAG302.1637898E-645.0000049
CAACCGC253.8886054E-545.00000445
TCCTCGG302.1637898E-645.0000042
ATTCGAC253.8886054E-545.00000422
TACGGGT750.045.0000044
TAACGCG302.1637898E-645.0000041
CTAACGC302.1637898E-645.00000418
TGCGCGA302.1637898E-645.00000429
TGCGCAC253.8886054E-545.00000429
CTAAACA302.1637898E-645.00000413
Produced by FastQC (version 0.11.5)