##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553318_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	604819
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503788736795638	31.0	31.0	34.0	30.0	34.0
2	31.687468482306276	31.0	31.0	34.0	30.0	34.0
3	31.871945160452963	33.0	31.0	34.0	30.0	34.0
4	35.610561176153524	37.0	35.0	37.0	33.0	37.0
5	35.36711148293952	37.0	35.0	37.0	33.0	37.0
6	34.09891554332784	37.0	35.0	37.0	32.0	37.0
7	34.92067048158209	37.0	35.0	37.0	32.0	37.0
8	35.586382041569465	37.0	35.0	37.0	33.0	37.0
9	37.263318447337134	39.0	37.0	39.0	34.0	39.0
10	36.584199570450004	38.0	35.0	39.0	32.0	39.0
11	36.63343082806592	39.0	35.0	39.0	32.0	39.0
12	36.71059936939812	39.0	35.0	39.0	32.0	39.0
13	36.69924886618972	39.0	35.0	39.0	32.0	39.0
14	37.81218843984729	39.0	37.0	41.0	33.0	41.0
15	37.785952491571855	39.0	37.0	41.0	33.0	41.0
16	37.716587937878934	39.0	37.0	41.0	32.0	41.0
17	37.68435846096105	39.0	37.0	41.0	33.0	41.0
18	37.52255798842298	39.0	36.0	40.0	32.0	41.0
19	37.41980493337676	39.0	36.0	41.0	32.0	41.0
20	37.30530952235297	39.0	35.0	40.0	32.0	41.0
21	37.26565964362892	39.0	35.0	40.0	32.0	41.0
22	37.21129627210785	39.0	35.0	40.0	32.0	41.0
23	37.0230829388627	39.0	35.0	40.0	31.0	41.0
24	36.82712513991789	39.0	35.0	40.0	31.0	41.0
25	36.911176732212446	39.0	35.0	40.0	31.0	41.0
26	36.825466792544546	39.0	35.0	40.0	31.0	41.0
27	36.77035609000379	39.0	35.0	40.0	31.0	41.0
28	36.73410888216144	39.0	35.0	40.0	31.0	41.0
29	36.547687820653785	38.0	35.0	40.0	31.0	41.0
30	36.436211494678574	38.0	35.0	40.0	30.0	41.0
31	36.40092655819344	38.0	35.0	40.0	30.0	41.0
32	36.28435945299338	38.0	35.0	40.0	30.0	41.0
33	36.21056712834749	38.0	35.0	40.0	30.0	41.0
34	36.157407422716545	38.0	35.0	40.0	30.0	41.0
35	35.95838589726844	38.0	35.0	40.0	30.0	41.0
36	35.80258556692167	38.0	35.0	40.0	29.0	41.0
37	35.71964339744618	38.0	35.0	40.0	29.0	41.0
38	35.68684350194025	38.0	35.0	40.0	29.0	41.0
39	35.64669760705269	38.0	35.0	40.0	28.0	41.0
40	35.46713810247363	38.0	34.0	40.0	27.0	41.0
41	35.35155641605174	38.0	34.0	40.0	27.0	41.0
42	35.20348401753252	38.0	34.0	40.0	26.0	41.0
43	35.076290592722785	38.0	34.0	40.0	26.0	41.0
44	34.89335487145741	38.0	33.0	40.0	25.0	41.0
45	34.6560458583477	37.0	33.0	40.0	24.0	41.0
46	34.48884376978898	37.0	33.0	40.0	24.0	41.0
47	34.38437615220421	37.0	33.0	40.0	23.0	41.0
48	34.21605968066479	37.0	33.0	40.0	23.0	41.0
49	34.04347747011916	36.0	33.0	40.0	23.0	41.0
50	33.94891198854533	36.0	33.0	40.0	23.0	41.0
51	32.7787867113963	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	7.0
13	8.0
14	19.0
15	33.0
16	90.0
17	165.0
18	374.0
19	660.0
20	1138.0
21	1764.0
22	2460.0
23	3312.0
24	4241.0
25	5118.0
26	5983.0
27	7254.0
28	9244.0
29	11480.0
30	14286.0
31	18084.0
32	23181.0
33	30298.0
34	42101.0
35	63613.0
36	65878.0
37	83015.0
38	107755.0
39	103241.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.85301553026608	33.80664297913921	20.10915662371718	25.231184866877527
2	21.944581767437864	36.189008612493986	19.643562784899284	22.22284683516887
3	20.314176637969375	35.437544124771215	20.10055901021628	24.14772022704313
4	19.190534688890395	36.77513437904563	19.079923084426913	24.95440784763706
5	16.43946370732401	38.93346604521353	16.727483759604116	27.89958648785835
6	17.873281097320024	43.47383266729385	17.251276828274246	21.40160940711188
7	80.55848113237182	11.794768352184704	3.8707448013372594	3.7760057141062036
8	82.2211273124687	11.078024995907866	3.241961644723463	3.458886046899982
9	75.47299274659031	14.883957018546045	5.982616286856068	3.660433948007586
10	27.18218177669683	46.65792575960742	11.446565005398309	14.71332745829744
11	17.55550007522912	34.13434432450039	30.34742625479689	17.962729345473605
12	21.860259019640587	28.66064062140905	32.99301113225609	16.48608922669427
13	23.222484743369503	29.66804944950473	28.38750105403435	18.72196475309142
14	18.005221396814584	34.43939426506112	28.33244325988436	19.222941078239934
15	15.233648413822978	38.3443641816808	26.743703488151	19.678283916345222
16	17.73720732979619	34.38764324533455	27.54592696327331	20.32922246159595
17	16.164174736574083	34.391611374642665	28.557634598119435	20.886579290663818
18	16.398790381915912	34.84811158379614	28.627407538453653	20.12569049583429
19	16.601330315350545	38.55120292186588	27.36884919289903	17.478617569884545
20	18.17320553752445	37.07340543203835	26.121368541662875	18.63202048877433
21	19.624548831964606	35.13315553909517	28.020614431755615	17.221681197184612
22	16.497497598455073	34.27984239913098	26.340938363378136	22.88172163903581
23	17.168111451525167	38.14661907116013	24.080592706247657	20.604676771067044
24	15.604999181573332	39.07631870030538	25.144216699541516	20.174465418579775
25	17.9736417010709	36.824901334118145	24.390272131001176	20.811184833809783
26	18.294564158864056	37.569752272994066	23.73404274667297	20.401640821468902
27	15.064010885901402	35.55840672994731	27.590072401826	21.78750998232529
28	17.931314988451092	35.72969764508059	28.117502922361897	18.22148444410642
29	18.39194866563385	36.98990937784693	25.586332439953107	19.031809516566113
30	20.080553024954572	35.215328883517216	26.15096417275251	18.5531539187757
31	20.116100850006365	35.94033917585261	24.050997075158023	19.892562898983
32	18.96368996344361	35.310233309469446	25.360149069391007	20.36592765769594
33	19.762937341584838	34.121117226806696	25.34047376157164	20.775471670036822
34	18.6940225092135	33.70429831073429	26.54810778100556	21.053571399046657
35	20.781589202720152	32.198889254471176	26.90623145106222	20.113290091746457
36	21.559507885830307	33.05798924967635	28.327152420806883	17.05535044368646
37	17.6970300205516	34.909121571908294	27.299902946170672	20.093945461369433
38	17.911474341910555	33.790605123185614	29.916388208703758	18.38153232620007
39	18.974602319040905	32.031731807367166	27.339914916694084	21.65375095689785
40	20.48893966624726	32.45351088507471	27.11951840137297	19.93803104730506
41	17.89907393782272	31.76140299825237	30.10421299595416	20.23531006797075
42	19.42581168911691	34.2705834307454	25.458194931045487	20.845409949092208
43	17.822191432478146	32.97135175978268	26.662852853498322	22.543603954240858
44	19.12092708727735	31.83001856753839	27.486735701094045	21.562318644090215
45	20.891870129741292	33.301202508519076	27.07206618839686	18.734861173342768
46	19.537911342070934	33.15405104667678	29.653995658205183	17.65404195304711
47	19.413411285029074	33.839214707209926	26.80471347626315	19.942660531497854
48	17.66263956654801	33.14396538468534	29.353905879279584	19.83948916948707
49	17.748615701556993	32.24220799941801	27.807988836329546	22.20118746269545
50	18.043745318847456	31.162050134007035	28.27242530409924	22.521779243046268
51	18.330608000079362	30.025511764676704	29.842481800340266	21.801398434903664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23922.0
1	17868.0
2	11814.0
3	7215.5
4	2617.0
5	2158.5
6	1700.0
7	1925.0
8	2150.0
9	2730.5
10	3311.0
11	4079.5
12	4848.0
13	5046.5
14	5245.0
15	5907.0
16	6569.0
17	6048.0
18	5527.0
19	5584.0
20	5641.0
21	5295.5
22	4950.0
23	5608.5
24	6267.0
25	6021.5
26	6706.0
27	7636.0
28	8924.0
29	10212.0
30	11861.5
31	13511.0
32	15031.5
33	16552.0
34	18603.0
35	20654.0
36	22285.5
37	23917.0
38	24555.0
39	25193.0
40	24947.0
41	24701.0
42	27929.0
43	31157.0
44	35690.0
45	40223.0
46	43670.0
47	47117.0
48	51851.5
49	56586.0
50	54244.5
51	51903.0
52	45710.0
53	39517.0
54	34120.0
55	28723.0
56	24674.5
57	20626.0
58	18510.5
59	16395.0
60	14394.5
61	12394.0
62	10035.5
63	7677.0
64	6940.0
65	6203.0
66	5079.5
67	3956.0
68	3534.0
69	3112.0
70	2749.0
71	2386.0
72	1922.5
73	1459.0
74	1346.0
75	840.5
76	448.0
77	455.0
78	462.0
79	343.5
80	225.0
81	176.0
82	127.0
83	103.5
84	80.0
85	70.0
86	60.0
87	34.0
88	8.0
89	8.0
90	8.0
91	8.5
92	9.0
93	9.5
94	10.0
95	6.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	604819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.572514394017162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91771316563208	21.691599521858887
2	9.768617222513413	5.582279123998168
3	3.652632071320918	3.1309464730159693
4	1.9806318234822338	2.263665251427783
5	1.2121230424568046	1.731670153895846
6	0.8995194586960088	1.542091960877414
7	0.7099866685400017	1.420027301449364
8	0.548001647164964	1.2526227961252836
9	0.4827857069178507	1.2414961404122435
>10	3.838001787673174	22.88247636507494
>50	0.6032644060221742	12.147906400560492
>100	0.3761600041441768	17.588055788637583
>500	0.007041996957457287	1.4384663769552584
>1k	0.0029341653989405363	2.5048664419319975
>5k	0.0	0.0
>10k+	5.868330797881073E-4	3.581829903778789
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21362	3.5319657616576197	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4471	0.7392294223561098	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4218	0.6973987258998147	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	3296	0.5449564249800354	TruSeq Adapter, Index 10 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1878	0.3105061183593769	No Hit
CAGAGAGAGGGAAAAGGATCGTGAAAAAGACAAGGAGCGGGGTAAAAACAA	1076	0.1779044639801329	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	993	0.16418135012292934	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	992	0.16401601140175823	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC	940	0.1554183979008596	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGT	873	0.14434070358239406	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	847	0.14004189683194476	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	664	0.10978491085762848	RNA PCR Primer, Index 35 (95% over 22bp)
AGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	628	0.1038327168954679	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7180660660462056	0.0
2	0.0	0.0	0.0	0.9131657570281357	0.0
3	0.0	0.0	0.0	1.1249646588483497	0.0
4	0.0	0.0	0.0	2.136506954973306	0.0
5	0.0	0.0	0.0	2.2980428855574973	0.0
6	0.0	0.0	0.0	3.2518819679937305	0.0
7	0.0	0.0	0.0	3.954075516807508	0.0
8	0.0	0.0	0.0	4.508786926336639	0.0
9	0.0	0.0	0.0	5.656072312543092	0.0
10	0.0	0.0	0.0	7.437431694440816	0.0
11	0.0	0.0	0.0	9.254504240111505	0.0
12	0.0	0.0	0.0	10.05027950510814	0.0
13	0.0	0.0	0.0	10.37169797906481	0.0
14	0.0	0.0	0.0	10.714114470610216	0.0
15	0.0	0.0	0.0	11.022140508152026	0.0
16	0.0	0.0	0.0	11.620666678791506	0.0
17	0.0	0.0	0.0	12.438431993703901	0.0
18	0.0	0.0	0.0	13.462870710080205	0.0
19	0.0	0.0	0.0	13.889444610701714	0.0
20	0.0	0.0	0.0	14.335528480421415	0.0
21	0.0	0.0	0.0	14.783265737352828	0.0
22	0.0	0.0	0.0	15.313010999985119	0.0
23	0.0	0.0	0.0	15.873178587312898	0.0
24	0.0	0.0	0.0	16.22997954760019	0.0
25	0.0	0.0	0.0	16.53932829491137	0.0
26	0.0	0.0	0.0	16.828671056960843	0.0
27	0.0	0.0	0.0	17.163647306053548	0.0
28	0.0	0.0	0.0	17.50887455585886	0.0
29	0.0	0.0	0.0	17.85641654776057	0.0
30	0.0	0.0	0.0	18.301343046432073	0.0
31	0.0	0.0	0.0	18.61928940724415	0.0
32	0.0	0.0	0.0	18.94517202667244	0.0
33	0.0	0.0	0.0	19.297839518930456	0.0
34	0.0	0.0	0.0	19.633807800350187	0.0
35	0.0	0.0	0.0	20.01954303684243	0.0
36	0.0	0.0	0.0	20.351047172790537	0.0
37	0.0	0.0	0.0	20.6696548884873	0.0
38	0.0	0.0	0.0	20.997852250011988	0.0
39	0.0	0.0	0.0	21.31381454617001	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	3.8886054E-5	45.000004	1
GATAATC	30	2.1637898E-6	45.000004	9
CATATCG	25	3.8886054E-5	45.000004	32
CGAGTTT	25	3.8886054E-5	45.000004	37
GATCGCG	30	2.1637898E-6	45.000004	1
ACGCATG	30	2.1637898E-6	45.000004	1
CGACGAA	25	3.8886054E-5	45.000004	39
GCGCGTA	30	2.1637898E-6	45.000004	34
TAGGTGT	30	2.1637898E-6	45.000004	20
TAGAACG	25	3.8886054E-5	45.000004	1
GACCGAG	30	2.1637898E-6	45.000004	9
CAACCGC	25	3.8886054E-5	45.000004	45
TCCTCGG	30	2.1637898E-6	45.000004	2
ATTCGAC	25	3.8886054E-5	45.000004	22
TACGGGT	75	0.0	45.000004	4
TAACGCG	30	2.1637898E-6	45.000004	1
CTAACGC	30	2.1637898E-6	45.000004	18
TGCGCGA	30	2.1637898E-6	45.000004	29
TGCGCAC	25	3.8886054E-5	45.000004	29
CTAAACA	30	2.1637898E-6	45.000004	13
>>END_MODULE