##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553317_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	599606
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47863763871609	31.0	31.0	34.0	30.0	34.0
2	31.677164671467597	31.0	31.0	34.0	30.0	34.0
3	31.85808681033879	33.0	31.0	34.0	30.0	34.0
4	35.60964533376917	37.0	35.0	37.0	33.0	37.0
5	35.382296041066965	37.0	35.0	37.0	33.0	37.0
6	32.813619276658336	37.0	35.0	37.0	27.0	37.0
7	34.29596434992312	37.0	35.0	37.0	29.0	37.0
8	35.326872979923486	37.0	35.0	37.0	32.0	37.0
9	37.14929470352198	39.0	37.0	39.0	34.0	39.0
10	36.55163223850328	38.0	35.0	39.0	32.0	39.0
11	36.64522202913246	39.0	35.0	39.0	32.0	39.0
12	36.721540478247384	39.0	35.0	39.0	32.0	39.0
13	36.732852906742096	39.0	37.0	39.0	32.0	39.0
14	37.80197829908307	39.0	37.0	41.0	33.0	41.0
15	37.834808190711904	39.0	37.0	41.0	33.0	41.0
16	37.795278899810874	39.0	37.0	41.0	33.0	41.0
17	37.779875451546516	39.0	37.0	41.0	33.0	41.0
18	37.611082944466865	39.0	37.0	41.0	33.0	41.0
19	37.51357724906022	39.0	36.0	41.0	32.0	41.0
20	37.38129038068332	39.0	36.0	40.0	32.0	41.0
21	37.32432130432317	39.0	35.0	40.0	32.0	41.0
22	37.230366273853164	39.0	35.0	40.0	32.0	41.0
23	37.054150558866986	39.0	35.0	40.0	32.0	41.0
24	36.92366987655227	39.0	35.0	40.0	31.0	41.0
25	36.96668645744039	39.0	35.0	40.0	31.0	41.0
26	36.88570494624803	39.0	35.0	40.0	31.0	41.0
27	36.81392781259694	39.0	35.0	40.0	31.0	41.0
28	36.782900771506625	39.0	35.0	40.0	31.0	41.0
29	36.5623993088795	38.0	35.0	40.0	31.0	41.0
30	36.475373828814256	38.0	35.0	40.0	30.0	41.0
31	36.106853500465306	38.0	35.0	40.0	30.0	41.0
32	36.19598369596035	38.0	35.0	40.0	30.0	41.0
33	36.16768010993886	38.0	35.0	40.0	30.0	41.0
34	36.16030026383992	38.0	35.0	40.0	30.0	41.0
35	36.070099365249845	38.0	35.0	40.0	30.0	41.0
36	35.91140849157613	38.0	35.0	40.0	30.0	41.0
37	35.79183830715503	38.0	35.0	40.0	29.0	41.0
38	35.737982942132	38.0	35.0	40.0	29.0	41.0
39	35.68257655860682	38.0	35.0	40.0	28.0	41.0
40	35.54317168273833	38.0	35.0	40.0	28.0	41.0
41	35.50765836232459	38.0	34.0	40.0	28.0	41.0
42	35.34098057724572	38.0	34.0	40.0	27.0	41.0
43	35.25661017401427	38.0	34.0	40.0	26.0	41.0
44	35.01924930704496	38.0	34.0	40.0	26.0	41.0
45	34.79816412777724	37.0	33.0	40.0	25.0	41.0
46	34.491706220418074	37.0	33.0	40.0	24.0	41.0
47	34.449208313459174	37.0	33.0	40.0	24.0	41.0
48	34.42418187943416	37.0	33.0	40.0	24.0	41.0
49	34.32809211382141	37.0	33.0	40.0	24.0	41.0
50	34.17662264887276	36.0	33.0	40.0	23.0	41.0
51	33.0718655250281	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	5.0
12	8.0
13	11.0
14	18.0
15	34.0
16	97.0
17	173.0
18	377.0
19	691.0
20	1129.0
21	1790.0
22	2418.0
23	3202.0
24	3961.0
25	4722.0
26	5708.0
27	6933.0
28	8587.0
29	10828.0
30	14147.0
31	17827.0
32	23058.0
33	30534.0
34	43120.0
35	64059.0
36	67057.0
37	83843.0
38	105592.0
39	99654.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.78915154284647	33.91610490889017	20.033655433734822	25.26108811452854
2	21.95858613823077	36.14606925214224	19.613212676324117	22.282131933302868
3	20.153567509331126	35.50748324733242	20.06067317538517	24.27827606795129
4	19.16775349145939	36.9357544787744	18.949109915511187	24.947382114255028
5	16.4861592445706	38.90654863360273	16.777016907769436	27.83027521405723
6	17.306030960330617	45.44317435115726	16.589894030413305	20.660900658098818
7	80.60943352801672	11.839107680710333	3.8493610804428244	3.7020977108301114
8	82.16512176329122	11.136646397801222	3.161742877823104	3.5364889610844457
9	75.49974483243996	14.95982361750883	5.920721273636355	3.6197102764148457
10	27.279413481519533	46.676484224640845	11.3234357227913	14.720666571048321
11	17.666100739485596	34.138584337047995	30.207502926922015	17.987811996544398
12	21.810488887702924	28.72136035996971	32.92128497713499	16.546865775192376
13	23.070316174287782	29.911141649683294	28.30592088804982	18.712621287979108
14	18.06136029325924	34.4919830688819	28.354953085859712	19.091703551999146
15	15.210488220598192	38.39354509461213	26.77107967565368	19.624887009136
16	17.795185505148382	34.43127653826012	27.482213320080184	20.29132463651131
17	16.19630223846993	34.37990947388786	28.54657892015757	20.877209367484646
18	16.473484254660562	34.856389028795576	28.512056250271012	20.158070466272854
19	16.563376617312034	38.57549791029442	27.187019476122654	17.67410599627088
20	18.256154875034607	37.09836125722558	26.163347264703823	18.482136603035993
21	19.767147093257904	35.09137667068041	28.050753328018736	17.09072290804295
22	16.667278179337767	34.29068421596849	26.299936958602814	22.742100646090933
23	17.272675723725246	38.10702361217199	24.17937779141636	20.4409228726864
24	15.683131923296298	39.05764785542507	25.173864170805494	20.085356050473145
25	17.979473187393054	36.892392671187416	24.356494097790883	20.77164004362865
26	18.2803374215735	37.65355917052198	23.763104438581333	20.302998969323188
27	15.089408711720697	35.6881018535505	27.564934306861506	21.6575551278673
28	17.924937375543273	35.83753331354256	28.0679312748705	18.16959803604367
29	18.33670777143658	37.16307041624	25.477230047731343	19.02299176459208
30	19.974616664943312	35.320360369976285	26.151506155708915	18.553516809371487
31	20.170245127633812	36.00514337748455	24.08198050052868	19.742630994352957
32	18.82469488297315	35.30835248479835	25.546108611321433	20.32084402090706
33	19.7217839714746	34.30402631061064	25.27426343298766	20.699926284927102
34	18.656584490482082	33.78285073865172	26.65283536188764	20.90772940897856
35	20.665236838857517	32.3245597942649	26.826782920784648	20.183420446092935
36	21.394382311050926	33.273349499504675	28.229203843857466	17.103064345586937
37	17.78301084378742	34.96729519050844	27.23121516462477	20.018478801079375
38	17.807693718875395	33.948292712214354	29.799735159421353	18.4442784094889
39	18.870891885671593	32.14143954530142	27.313269046673984	21.67439952235301
40	20.425245911481873	32.63909967545355	27.171008962552072	19.764645450512504
41	17.934443617975802	31.872596338262127	30.02088037811496	20.172079665647107
42	19.29283562872953	34.477306764775534	25.541105325830628	20.688752280664303
43	17.88057491085813	33.075719722617855	26.629486696263882	22.414218670260137
44	19.209280761033078	31.99734492316621	27.4226742227396	21.37070009306111
45	20.780479181329074	33.45029902969617	27.00156436059679	18.76765742837797
46	19.72895534734476	33.211141983235656	29.49253343028589	17.567369239133697
47	19.32902606044636	34.03401567029016	26.754735609717045	19.882222659546436
48	17.71746780385787	33.36691093818274	29.31908619993796	19.596535058021434
49	17.632078398148117	32.438134374906184	27.83527849954804	22.094508727397656
50	18.00565704812827	31.410459535094716	28.1983502499975	22.38553316677952
51	18.295347278045917	30.276881819061185	29.667481646281058	21.76028925661184
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24080.0
1	17986.0
2	11892.0
3	7247.5
4	2603.0
5	2121.0
6	1639.0
7	1915.5
8	2192.0
9	2748.5
10	3305.0
11	3976.0
12	4647.0
13	5012.5
14	5378.0
15	5906.5
16	6435.0
17	6002.0
18	5569.0
19	5591.5
20	5614.0
21	5307.5
22	5001.0
23	5645.5
24	6290.0
25	6075.5
26	6726.0
27	7591.0
28	8817.0
29	10043.0
30	11774.0
31	13505.0
32	14887.0
33	16269.0
34	18498.0
35	20727.0
36	22267.0
37	23807.0
38	24388.0
39	24969.0
40	24962.0
41	24955.0
42	27997.5
43	31040.0
44	35273.0
45	39506.0
46	43094.5
47	46683.0
48	51146.0
49	55609.0
50	53352.0
51	51095.0
52	44996.5
53	38898.0
54	33581.5
55	28265.0
56	24179.5
57	20094.0
58	18284.0
59	16474.0
60	14380.5
61	12287.0
62	9959.0
63	7631.0
64	6982.5
65	6334.0
66	5110.5
67	3887.0
68	3477.5
69	3068.0
70	2713.0
71	2358.0
72	1906.5
73	1455.0
74	1324.0
75	834.5
76	476.0
77	452.5
78	429.0
79	310.5
80	192.0
81	154.5
82	117.0
83	90.0
84	63.0
85	52.0
86	41.0
87	25.5
88	10.0
89	11.0
90	12.0
91	11.5
92	11.0
93	7.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	599606.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.795878013605225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.16774299302979	21.645211577853146
2	10.043769961258128	5.784383492022031
3	3.7411635985387903	3.2319027183739
4	2.056002670135577	2.368176083394828
5	1.3359927175151831	1.9235541660316078
6	0.9313477948452421	1.6091386493162292
7	0.7608965357844468	1.533747867779661
8	0.6128327468249393	1.4117645616250851
9	0.4699409994020602	1.2179127323136105
>10	3.924924706778965	23.294001952691744
>50	0.5872981879991814	11.824396916480069
>100	0.3587090729096797	16.843917375187388
>500	0.006447385297396204	1.383828118390763
>1k	0.0023445037445077106	2.3200513861933687
>5k	0.0	0.0
>10k+	5.861259361269277E-4	3.6080124023465476
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21377	3.5651744645650645	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4367	0.7283115912782727	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4286	0.7148027204530975	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	3123	0.5208420195928659	TruSeq Adapter, Index 10 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1970	0.328549080562903	No Hit
CAGAGAGAGGGAAAAGGATCGTGAAAAAGACAAGGAGCGGGGTAAAAACAA	980	0.16344065936631721	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	949	0.15827059769248472	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	888	0.1480972505278466	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC	884	0.1474301457957392	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	840	0.14009199374255762	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGT	805	0.13425482733661773	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	663	0.1105726093468044	RNA PCR Primer, Index 35 (95% over 22bp)
AGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	636	0.10606965240507933	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.667761830268543E-4	0.0	0.0	0.6664376273753098	0.0
2	1.667761830268543E-4	0.0	0.0	0.8672361517396424	0.0
3	1.667761830268543E-4	0.0	0.0	1.0762067090722909	0.0
4	1.667761830268543E-4	0.0	0.0	2.061853950761	0.0
5	1.667761830268543E-4	0.0	0.0	2.218456786623216	0.0
6	1.667761830268543E-4	0.0	0.0	3.1172136369549337	0.0
7	1.667761830268543E-4	0.0	0.0	3.793491059128828	0.0
8	1.667761830268543E-4	0.0	0.0	4.324839978252386	0.0
9	1.667761830268543E-4	0.0	0.0	5.4230611434842215	0.0
10	1.667761830268543E-4	0.0	0.0	7.122010119978786	0.0
11	1.667761830268543E-4	0.0	0.0	8.90851659256245	0.0
12	1.667761830268543E-4	0.0	0.0	9.714712661314262	0.0
13	1.667761830268543E-4	0.0	0.0	10.035423261274904	0.0
14	1.667761830268543E-4	0.0	0.0	10.373812136636392	0.0
15	1.667761830268543E-4	0.0	0.0	10.666671114031548	0.0
16	1.667761830268543E-4	0.0	0.0	11.245217692951705	0.0
17	1.667761830268543E-4	0.0	0.0	12.035069695766888	0.0
18	1.667761830268543E-4	0.0	0.0	13.068081373435222	0.0
19	1.667761830268543E-4	0.0	0.0	13.47801723131523	0.0
20	1.667761830268543E-4	0.0	0.0	13.911134978635971	0.0
21	1.667761830268543E-4	0.0	0.0	14.372104348522196	0.0
22	1.667761830268543E-4	0.0	0.0	14.899450639253109	0.0
23	1.667761830268543E-4	0.0	0.0	15.432634096389963	0.0
24	1.667761830268543E-4	0.0	0.0	15.794038085009156	0.0
25	1.667761830268543E-4	0.0	0.0	16.09556942392171	0.0
26	1.667761830268543E-4	0.0	0.0	16.38225768254487	0.0
27	1.667761830268543E-4	0.0	0.0	16.71564327241555	0.0
28	1.667761830268543E-4	0.0	0.0	17.04919563846926	0.0
29	1.667761830268543E-4	0.0	0.0	17.407264103427917	0.0
30	1.667761830268543E-4	0.0	0.0	17.841716060212875	0.0
31	1.667761830268543E-4	0.0	0.0	18.16959803604367	0.0
32	1.667761830268543E-4	0.0	0.0	18.486472783794692	0.0
33	1.667761830268543E-4	0.0	0.0	18.831032377928175	0.0
34	1.667761830268543E-4	0.0	0.0	19.143904497286552	0.0
35	1.667761830268543E-4	0.0	0.0	19.54583509838127	0.0
36	1.667761830268543E-4	0.0	0.0	19.865878593609803	0.0
37	1.667761830268543E-4	0.0	0.0	20.17308032274527	0.0
38	1.667761830268543E-4	0.0	0.0	20.50279683658936	0.0
39	1.667761830268543E-4	0.0	0.0	20.82567552692935	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGC	45	3.8380676E-10	45.000004	18
CCCGTTC	45	3.8380676E-10	45.000004	16
GTAAGCG	45	3.8380676E-10	45.000004	1
TACAACG	55	1.8189894E-12	45.000004	1
CTTCGAT	20	7.030585E-4	45.0	21
CAGGTCG	20	7.030585E-4	45.0	23
CTATGCG	20	7.030585E-4	45.0	1
CGAACAC	20	7.030585E-4	45.0	29
ACTGCCG	20	7.030585E-4	45.0	1
TCCGCTC	35	1.2106284E-7	45.0	11
GTCGATG	20	7.030585E-4	45.0	1
TACTTCG	25	3.888566E-5	45.0	18
TTGTGCG	35	1.2106284E-7	45.0	1
CTCCGGA	35	1.2106284E-7	45.0	3
GCGAATT	20	7.030585E-4	45.0	23
CGTGCCT	25	3.888566E-5	45.0	23
CGTGAAT	35	1.2106284E-7	45.0	27
CACGCAT	25	3.888566E-5	45.0	43
GGATCAC	100	0.0	45.0	8
ACTTCGT	25	3.888566E-5	45.0	19
>>END_MODULE