FastQCFastQC Report
Sat 18 Jun 2016
SRR3553315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711214
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA143842.022457375698454No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37250.5237523445826432No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26220.3686654087236753No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT15990.22482684536581113RNA PCR Primer, Index 34 (96% over 26bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15400.2165311706462471No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAT302.16433E-645.00000415
CCGATTC351.2110468E-745.00000439
GCAACAC302.16433E-645.00000429
CTCCCGT302.16433E-645.00000413
CAACCGT302.16433E-645.00000411
CGACCGA302.16433E-645.00000429
TTCGTTG302.16433E-645.00000411
TAAGTCG351.2110468E-745.0000042
CACGACA302.16433E-645.00000418
CGTTCGA302.16433E-645.00000413
ATATCCG351.2110468E-745.0000041
TCTACGG700.045.0000042
TACGACG302.16433E-645.0000041
GTCACTA351.2110468E-745.00000436
TCGCTAG302.16433E-645.00000416
ACGTGCG302.16433E-645.00000413
GCGATAG650.045.0000041
TTGCACG302.16433E-645.0000042
CCAAACG600.045.0000041
CGATACG351.2110468E-745.0000041
Produced by FastQC (version 0.11.5)