##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	711214
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66364413523918	31.0	31.0	34.0	30.0	34.0
2	31.874361303348923	33.0	31.0	34.0	30.0	34.0
3	32.03718711948865	33.0	31.0	34.0	30.0	34.0
4	35.7691777720911	37.0	35.0	37.0	35.0	37.0
5	35.530228595050154	37.0	35.0	37.0	33.0	37.0
6	32.80937242517723	37.0	35.0	37.0	26.0	37.0
7	34.27062600005062	37.0	35.0	37.0	28.0	37.0
8	35.356503105956854	37.0	35.0	37.0	32.0	37.0
9	37.214256187307896	39.0	37.0	39.0	34.0	39.0
10	36.734282508499554	39.0	37.0	39.0	32.0	39.0
11	36.76445626773376	39.0	37.0	39.0	32.0	39.0
12	36.6902085729471	39.0	35.0	39.0	32.0	39.0
13	36.66779337864553	39.0	35.0	39.0	32.0	39.0
14	37.826162027181695	40.0	37.0	41.0	33.0	41.0
15	37.88240670178033	40.0	37.0	41.0	33.0	41.0
16	37.8219242028419	40.0	37.0	41.0	33.0	41.0
17	37.78425059124258	39.0	37.0	41.0	33.0	41.0
18	37.67908533859007	39.0	36.0	41.0	33.0	41.0
19	37.65120905943921	39.0	36.0	41.0	33.0	41.0
20	37.531359056486515	39.0	36.0	41.0	32.0	41.0
21	37.48797548979632	39.0	36.0	41.0	32.0	41.0
22	37.40999474138586	39.0	36.0	41.0	32.0	41.0
23	37.25545616368632	39.0	35.0	41.0	32.0	41.0
24	37.11285210921045	39.0	35.0	41.0	31.0	41.0
25	37.134314003942556	39.0	35.0	41.0	31.0	41.0
26	37.067796753157275	39.0	35.0	40.0	31.0	41.0
27	36.98232458866108	39.0	35.0	40.0	31.0	41.0
28	36.94212009324901	39.0	35.0	40.0	31.0	41.0
29	36.74550135402284	39.0	35.0	40.0	31.0	41.0
30	36.76090459411654	39.0	35.0	40.0	31.0	41.0
31	36.38768500057648	39.0	35.0	40.0	30.0	41.0
32	36.490197043365285	39.0	35.0	40.0	30.0	41.0
33	36.47986822531615	39.0	35.0	40.0	30.0	41.0
34	36.389407407615714	39.0	35.0	40.0	30.0	41.0
35	36.303728554274805	38.0	35.0	40.0	30.0	41.0
36	36.231438076303334	38.0	35.0	40.0	30.0	41.0
37	36.1536330837132	38.0	35.0	40.0	30.0	41.0
38	36.037417711124924	38.0	35.0	40.0	30.0	41.0
39	35.97711799823963	38.0	35.0	40.0	30.0	41.0
40	35.82422449501838	38.0	35.0	40.0	29.0	41.0
41	35.81406721464988	38.0	35.0	40.0	29.0	41.0
42	35.68596793651419	38.0	34.0	40.0	29.0	41.0
43	35.62622783016082	38.0	34.0	40.0	28.0	41.0
44	35.45533974303093	38.0	34.0	40.0	28.0	41.0
45	35.2536015882702	38.0	34.0	40.0	27.0	41.0
46	34.92010843431091	37.0	34.0	40.0	26.0	41.0
47	34.87513322291181	37.0	34.0	40.0	26.0	41.0
48	34.886291608432906	37.0	34.0	40.0	26.0	41.0
49	34.752237723104436	37.0	33.0	40.0	26.0	41.0
50	34.633297432277764	37.0	33.0	40.0	26.0	41.0
51	33.581055772242955	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	8.0
11	12.0
12	12.0
13	26.0
14	44.0
15	72.0
16	102.0
17	213.0
18	438.0
19	763.0
20	1253.0
21	1814.0
22	2474.0
23	3233.0
24	4053.0
25	4960.0
26	6044.0
27	7549.0
28	9416.0
29	11963.0
30	15224.0
31	19963.0
32	25621.0
33	35113.0
34	50927.0
35	67810.0
36	75177.0
37	100914.0
38	132662.0
39	133330.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.1044580112315	32.38040870961483	19.422564797655838	26.092568481497835
2	25.01258411673561	30.151121884552328	19.53448610404182	25.30180789467024
3	20.859684989328105	32.618874206638225	19.80655611391227	26.714884690121398
4	21.54949143295829	33.151484644565485	18.500057647909067	26.79896627456715
5	18.181869310784098	36.10024549572984	16.030336860635476	29.68754833285059
6	17.295778767009647	45.12650200924054	16.961983313039394	20.61573591071042
7	84.39724189906272	8.729440084137826	3.618882642917603	3.2544353738818415
8	85.87499683639524	7.740708141290807	3.036216947360429	3.34807807495353
9	78.91998751430653	11.008641562173974	5.555149364326349	4.516221559193155
10	35.94951730421504	35.04655420168895	12.453354405284484	16.550574088811526
11	25.494155064439113	31.516533701530058	23.28019414690937	19.70911708712146
12	25.206056123754593	27.376148388530037	27.98848166655887	19.4293138211565
13	24.272722415475513	27.88373119764234	26.91201241820324	20.931533968678906
14	21.04162741453346	30.191053606931245	27.164819590165546	21.602499388369743
15	19.86181374382394	31.783823153087535	26.665532455772805	21.688830647315715
16	21.250706538397726	30.552126364216676	26.93240009336149	21.264767004024105
17	21.618668923840083	29.776972894234365	27.125590891067947	21.478767290857604
18	22.952304088502196	30.13706141892595	24.612845078977635	22.29778941359422
19	21.59814064402557	32.20465288928508	24.589785915350372	21.60742055133898
20	22.440784349014503	30.343187845008675	25.116631562370817	22.099396243606005
21	24.31012325404168	29.248439991338753	26.226564718917235	20.21487203570233
22	22.409429510667675	28.1584726959818	25.08443309608641	24.347664697264115
23	21.056672112753684	31.358775277202078	24.908255461787874	22.67629714825636
24	20.411859159127914	31.971108555230916	26.652596827396536	20.964435458244633
25	22.265028528684756	32.20999586622311	23.58657169290818	21.938403912183958
26	23.13396530439502	30.44821952323773	24.102168967427527	22.315646204939725
27	20.26352124676961	30.067462114075372	25.867319822163232	23.80169681699179
28	21.05723453137874	31.168255967964637	26.164839274817425	21.6096702258392
29	21.39384207847427	30.694137067043116	25.577814834916072	22.334206019566544
30	22.823510223364558	29.285981434561187	26.357467653898826	21.53304068817543
31	23.86679677284193	30.092911556859114	22.990407950349685	23.04988371994927
32	22.179822106988894	29.010115098971617	25.92806103366919	22.882001760370297
33	23.139308281333047	28.73663904253853	24.631404893604454	23.492647782523964
34	21.205572443737047	30.431909383111133	26.159496297879404	22.203021875272423
35	22.01362740328509	30.099801185016045	25.817545773845847	22.069025637853024
36	24.583318101162238	30.299178587598107	24.572210333317397	20.54529297792226
37	21.74971246347794	31.34851113729482	23.94876928744372	22.953007111783513
38	21.532478269550374	31.247135180128627	25.73951018961944	21.48087636070156
39	21.50815366401674	28.34674233071902	25.689173722677	24.45593028258724
40	21.00619504115498	30.630302553099348	25.925951963825234	22.437550441920433
41	20.688850331967593	29.601217073904618	26.903997952796203	22.805934641331582
42	22.033030845849492	29.06945026391494	25.346660780018393	23.550858110217177
43	21.749993672790467	28.694598250315657	27.057397632780006	22.498010444113866
44	23.765561420331995	27.08045679640727	26.411038027935334	22.742943755325403
45	24.077984966550154	27.700945144499407	25.94605842967096	22.275011459279487
46	21.895097678054707	29.473969859985882	26.9366182330494	21.694314228910006
47	21.467659523012763	30.297913145691734	26.920026883610277	21.314400447685227
48	21.741838602727167	29.23381710708732	27.37108662090454	21.653257669280976
49	21.797658651263895	29.67405028584927	26.42298942371776	22.105301639169085
50	21.13569192957394	28.779804672011515	26.930572232830063	23.153931165584478
51	21.548929014333236	27.669590306152582	26.75762850562559	24.023852173888592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16423.0
1	12567.0
2	8711.0
3	5263.5
4	1816.0
5	1535.5
6	1255.0
7	1267.0
8	1279.0
9	1441.0
10	1603.0
11	1723.5
12	1844.0
13	1859.0
14	1874.0
15	1903.0
16	1932.0
17	1879.0
18	1826.0
19	2023.5
20	2221.0
21	2551.0
22	2881.0
23	3222.0
24	3563.0
25	4434.5
26	6161.5
27	7017.0
28	8762.5
29	10508.0
30	11620.5
31	12733.0
32	15970.0
33	19207.0
34	19961.0
35	20715.0
36	23462.0
37	26209.0
38	27253.0
39	28297.0
40	30656.0
41	33015.0
42	33285.0
43	33555.0
44	35607.0
45	37659.0
46	41604.5
47	45550.0
48	45996.5
49	46443.0
50	51332.5
51	56222.0
52	52051.0
53	47880.0
54	45007.5
55	42135.0
56	38436.5
57	34738.0
58	33615.0
59	32492.0
60	29377.0
61	26262.0
62	25425.0
63	24588.0
64	21011.0
65	17434.0
66	16277.0
67	15120.0
68	13222.0
69	11324.0
70	10545.5
71	9767.0
72	8757.0
73	7747.0
74	6435.0
75	4375.5
76	3628.0
77	2679.0
78	1730.0
79	1261.5
80	793.0
81	618.5
82	444.0
83	314.5
84	185.0
85	127.5
86	70.0
87	53.0
88	36.0
89	34.0
90	32.0
91	23.0
92	14.0
93	9.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	711214.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.51493588546931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.19600648282115	16.608671300921703
2	8.826019943626708	3.7978250642200417
3	3.177329613474416	2.0508012876271517
4	1.7036125444187211	1.466124586674001
5	1.1372293367641597	1.2233708133777843
6	0.7772698175340221	1.003374617397295
7	0.5860138770533491	0.8825635694958669
8	0.4720697756588658	0.8125240765414704
9	0.3692558801251717	0.7150064927603049
>10	3.621169312426102	17.472306649412882
>50	1.2098973428524424	18.899915712425237
>100	0.9161879824224346	31.037215118699706
>500	0.004630552979752377	0.6330523892953662
>1k	0.0026460302741442157	1.3500753068470872
>5k	0.0	0.0
>10k+	6.615075685360539E-4	2.04717301430408
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14384	2.022457375698454	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3725	0.5237523445826432	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2622	0.3686654087236753	No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	1599	0.22482684536581113	RNA PCR Primer, Index 34 (96% over 26bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1540	0.2165311706462471	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4060465626379684E-4	0.0	0.0	0.27305424246429344	0.0
2	1.4060465626379684E-4	0.0	0.0	0.36683754819224595	0.0
3	1.4060465626379684E-4	0.0	0.0	0.47988369182833857	0.0
4	1.4060465626379684E-4	0.0	0.0	0.9918252452848229	0.0
5	1.4060465626379684E-4	0.0	0.0	1.0701420388237577	0.0
6	1.4060465626379684E-4	0.0	0.0	1.548197870120667	0.0
7	1.4060465626379684E-4	0.0	0.0	1.870041928308498	0.0
8	1.4060465626379684E-4	0.0	0.0	2.1101946812070627	0.0
9	1.4060465626379684E-4	0.0	0.0	2.5951401406608983	0.0
10	1.4060465626379684E-4	0.0	0.0	3.2957731428233976	0.0
11	1.4060465626379684E-4	0.0	0.0	4.036337867364816	0.0
12	1.4060465626379684E-4	0.0	0.0	4.335825785206703	0.0
13	1.4060465626379684E-4	0.0	0.0	4.468275371407199	0.0
14	1.4060465626379684E-4	0.0	0.0	4.616894493078033	0.0
15	1.4060465626379684E-4	0.0	0.0	4.751031335153695	0.0
16	1.4060465626379684E-4	0.0	0.0	5.030412787149859	0.0
17	1.4060465626379684E-4	0.0	0.0	5.362661589901212	0.0
18	1.4060465626379684E-4	0.0	0.0	5.808378350257447	0.0
19	1.4060465626379684E-4	0.0	0.0	5.981322077461917	0.0
20	1.4060465626379684E-4	0.0	0.0	6.212335527703336	0.0
21	1.4060465626379684E-4	0.0	0.0	6.4290073030058466	0.0
22	1.4060465626379684E-4	0.0	0.0	6.671128521092104	0.0
23	1.4060465626379684E-4	0.0	0.0	6.920701785960344	0.0
24	1.4060465626379684E-4	0.0	0.0	7.102081792540642	0.0
25	1.4060465626379684E-4	0.0	0.0	7.2703855660884065	0.0
26	1.4060465626379684E-4	0.0	0.0	7.43418999063573	0.0
27	1.4060465626379684E-4	0.0	0.0	7.596307159307887	0.0
28	1.4060465626379684E-4	0.0	0.0	7.776702933294339	0.0
29	1.4060465626379684E-4	0.0	0.0	7.960473219031122	0.0
30	1.4060465626379684E-4	0.0	0.0	8.186846715615834	0.0
31	1.4060465626379684E-4	0.0	0.0	8.350229326194366	0.0
32	2.812093125275937E-4	0.0	0.0	8.537514728337744	0.0
33	2.812093125275937E-4	0.0	0.0	8.712989339354962	0.0
34	2.812093125275937E-4	0.0	0.0	8.889729392278555	0.0
35	2.812093125275937E-4	0.0	0.0	9.085732283110287	0.0
36	2.812093125275937E-4	0.0	0.0	9.263737777940253	0.0
37	2.812093125275937E-4	0.0	0.0	9.442165086739012	0.0
38	2.812093125275937E-4	0.0	0.0	9.633949837882831	0.0
39	2.812093125275937E-4	0.0	0.0	9.825593984370386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	30	2.16433E-6	45.000004	15
CCGATTC	35	1.2110468E-7	45.000004	39
GCAACAC	30	2.16433E-6	45.000004	29
CTCCCGT	30	2.16433E-6	45.000004	13
CAACCGT	30	2.16433E-6	45.000004	11
CGACCGA	30	2.16433E-6	45.000004	29
TTCGTTG	30	2.16433E-6	45.000004	11
TAAGTCG	35	1.2110468E-7	45.000004	2
CACGACA	30	2.16433E-6	45.000004	18
CGTTCGA	30	2.16433E-6	45.000004	13
ATATCCG	35	1.2110468E-7	45.000004	1
TCTACGG	70	0.0	45.000004	2
TACGACG	30	2.16433E-6	45.000004	1
GTCACTA	35	1.2110468E-7	45.000004	36
TCGCTAG	30	2.16433E-6	45.000004	16
ACGTGCG	30	2.16433E-6	45.000004	13
GCGATAG	65	0.0	45.000004	1
TTGCACG	30	2.16433E-6	45.000004	2
CCAAACG	60	0.0	45.000004	1
CGATACG	35	1.2110468E-7	45.000004	1
>>END_MODULE