FastQCFastQC Report
Sat 18 Jun 2016
SRR3553313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525802
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA163651.0725506979280404No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGT72310.47391470190758694No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCC62460.4093584881917837No Hit
AATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT58120.38091443057487145No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT45330.2970896616992244Illumina Single End Adapter 1 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36400.23856306388378049No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29460.19307878741802673No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG27520.1803641625846604No Hit
AATGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT17450.11436608419703213No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15720.10302778473222608No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA207.034013E-445.00000419
CGTATAG406.8175723E-945.0000041
CTATCGA207.034013E-445.0000041
TCGATTG207.034013E-445.00000441
TTGCGAC207.034013E-445.00000440
TACTCGC650.045.00000445
CCGAACG207.034013E-445.0000041
ATTGCGT207.034013E-445.00000414
CAATCGT207.034013E-445.00000424
TCGACAC302.1659707E-645.00000433
GTAACGA207.034013E-445.00000429
ACAACGT253.891409E-545.034
GTCGAAC351.2122837E-745.039
CGACATA453.8562575E-1045.025
ATCGTTA351.2122837E-745.028
AATTGCG351.2122837E-745.01
CGCTATA351.2122837E-745.036
ACTCGAT253.891409E-545.021
TCGTACC253.891409E-545.042
CGAGACG453.8562575E-1045.01