##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553313_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1525802
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.610819752497374	31.0	31.0	34.0	30.0	34.0
2	31.825500949664505	33.0	31.0	34.0	30.0	34.0
3	31.975300202778605	33.0	31.0	34.0	30.0	34.0
4	35.70580520932598	37.0	35.0	37.0	33.0	37.0
5	35.463208856719284	37.0	35.0	37.0	33.0	37.0
6	32.57249105716207	37.0	35.0	37.0	25.0	37.0
7	34.151178855447824	37.0	35.0	37.0	26.0	37.0
8	35.31364685588301	37.0	35.0	37.0	32.0	37.0
9	37.19559484127036	39.0	37.0	39.0	34.0	39.0
10	36.64293925424138	38.0	35.0	39.0	32.0	39.0
11	36.70057713910455	39.0	37.0	39.0	32.0	39.0
12	36.607329129205496	39.0	35.0	39.0	32.0	39.0
13	36.62167961504835	39.0	35.0	39.0	32.0	39.0
14	37.74400282605475	39.0	37.0	41.0	33.0	41.0
15	37.798342773177644	40.0	37.0	41.0	33.0	41.0
16	37.70012295173292	39.0	36.0	41.0	33.0	41.0
17	37.69162447027858	39.0	36.0	41.0	33.0	41.0
18	37.58988256667641	39.0	36.0	41.0	33.0	41.0
19	37.57116126469883	39.0	36.0	41.0	32.0	41.0
20	37.501203957000975	39.0	36.0	41.0	32.0	41.0
21	37.48968083670096	39.0	36.0	41.0	32.0	41.0
22	37.42146752986299	39.0	36.0	41.0	32.0	41.0
23	37.296737715640695	39.0	36.0	41.0	32.0	41.0
24	37.137629915283895	39.0	35.0	40.0	31.0	41.0
25	37.20661789668647	39.0	35.0	40.0	32.0	41.0
26	37.09620252169023	39.0	35.0	40.0	31.0	41.0
27	36.97477850992462	39.0	35.0	40.0	31.0	41.0
28	36.91013512893547	39.0	35.0	40.0	31.0	41.0
29	36.73367710882539	39.0	35.0	40.0	31.0	41.0
30	36.74401331234328	39.0	35.0	40.0	31.0	41.0
31	36.37849013174711	39.0	35.0	40.0	30.0	41.0
32	36.45825342999944	39.0	35.0	40.0	30.0	41.0
33	36.45469726740429	39.0	35.0	40.0	30.0	41.0
34	36.37836691785697	39.0	35.0	40.0	30.0	41.0
35	36.32526697435185	39.0	35.0	40.0	30.0	41.0
36	36.26007109703618	38.0	35.0	40.0	30.0	41.0
37	36.1927851713394	38.0	35.0	40.0	30.0	41.0
38	36.096266094814396	38.0	35.0	40.0	30.0	41.0
39	36.06400896053354	38.0	35.0	40.0	30.0	41.0
40	35.88251162339544	38.0	35.0	40.0	29.0	41.0
41	35.9031958275058	38.0	35.0	40.0	29.0	41.0
42	35.796718053849716	38.0	35.0	40.0	29.0	41.0
43	35.75950352666991	38.0	35.0	40.0	29.0	41.0
44	35.607703358627134	38.0	34.0	40.0	28.0	41.0
45	35.444857196412116	38.0	34.0	40.0	28.0	41.0
46	35.18203213785275	38.0	34.0	40.0	27.0	41.0
47	35.149360795175255	37.0	34.0	40.0	27.0	41.0
48	35.147469330883034	37.0	34.0	40.0	27.0	41.0
49	35.017253221584454	37.0	34.0	40.0	27.0	41.0
50	34.89873981027682	37.0	34.0	40.0	26.0	41.0
51	33.72762455416889	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	9.0
9	22.0
10	18.0
11	23.0
12	26.0
13	28.0
14	55.0
15	84.0
16	191.0
17	377.0
18	675.0
19	1302.0
20	2417.0
21	3866.0
22	5364.0
23	6823.0
24	8523.0
25	10224.0
26	12551.0
27	15741.0
28	19751.0
29	25472.0
30	32999.0
31	43166.0
32	57027.0
33	76700.0
34	109863.0
35	140305.0
36	162924.0
37	218084.0
38	286324.0
39	284809.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.388031998909426	33.17789595242371	20.239913173531036	26.19415887513583
2	24.73289456954441	31.11045863093639	19.15025671745089	25.006390082068314
3	21.329635168914447	29.698283263490282	20.982211322307876	27.989870245287396
4	21.952651785749396	32.251039125653264	18.329704640575905	27.466604448021435
5	18.237556380185634	36.88447124856305	16.643968221302632	28.23400414994868
6	18.66795298472541	42.079116425329104	18.465174380424195	20.787756209521287
7	84.29501337657179	7.802257435761652	4.068286710857634	3.834442476808918
8	85.95853197203832	5.534794160710237	3.4929171674961754	5.013756699755277
9	79.94877448056825	9.542653633957748	5.993176047744072	4.515395837729928
10	35.19624433576571	36.65088917172739	13.046712483008935	15.106154009497955
11	25.083595381314222	30.214208658790593	25.112891449873576	19.589304510021616
12	25.819405139067847	25.73531821297914	30.02355482559336	18.42172182235965
13	24.889271347134162	27.572647040703842	26.81979706410137	20.718284548060627
14	21.598018615783698	27.978859642338914	29.573561969377415	20.84955977249997
15	19.492961734222398	31.986915733496218	26.129668200723295	22.390454331558093
16	22.170438890498243	28.7692636397121	27.36128278767494	21.69901468211472
17	21.862469704457066	28.879041972680596	28.131959454765425	21.12652886809691
18	22.476572976048008	29.0278817303949	26.780473482142508	21.71507181141459
19	21.119843859163904	31.46889308049144	26.830807667049854	20.580455393294805
20	23.542635282952833	31.202279194810334	25.43921164082889	19.815873881407942
21	23.334547995087174	29.528012153608397	27.255436812902328	19.8820030384021
22	22.204912564015515	27.930753793742568	26.519233819329113	23.3450998229128
23	21.301322189904063	30.776077105679505	25.482598659590174	22.44000204482626
24	19.85113402656439	32.22403693270818	27.262646136261452	20.66218290446598
25	21.27137072831206	30.145720086878903	25.372164933589026	23.210744251220014
26	23.668274127311406	30.499370167295623	23.72516224254523	22.107193462847736
27	20.419621943083047	30.108690380534302	26.724633995760914	22.747053680621732
28	20.884426681836832	31.176325630717482	26.56694643210587	21.372301255339814
29	21.073376493149176	32.07008510933922	25.30734656266016	21.54919183485144
30	22.42197873642845	30.214667433913444	25.300858171636946	22.06249565802116
31	23.818096974574683	31.126319142326466	23.28572121415492	21.76986266894394
32	21.75629603316813	28.91954526209823	25.45926666762791	23.86489203710573
33	21.69069119060009	30.094664969635645	24.409195950719685	23.805447889044583
34	20.682500088478058	29.78833426617608	26.11498739679198	23.414178248553874
35	20.262393154550853	30.136675663028363	27.216637545369583	22.384293637051204
36	22.181777189963046	32.125531359901224	25.051350044107952	20.64134140602778
37	20.626398444883414	32.251497900776116	24.354929407616453	22.767174246724018
38	21.320394127154113	31.417051491609005	25.727387957284105	21.53516642395278
39	21.361225113088068	27.959132312056216	26.18498337267876	24.49465920217695
40	21.871120892488015	29.66072924271957	25.986006047966903	22.482143816825513
41	21.50482172654119	28.165712195946785	28.622455600399004	21.70701047711302
42	22.216381942086848	28.558685858322374	25.73466281994649	23.49026937964428
43	21.268093763148823	28.761005687500734	27.118066433259365	22.852834116091078
44	21.24482731048983	27.431803077987837	27.311210759980654	24.01215885154168
45	23.247773957564615	29.17940859954306	26.362660423829563	21.210157019062763
46	20.424144155008317	28.46771730539087	27.986396662214364	23.12174187738645
47	21.168605100792895	30.431864684932908	26.522576323795615	21.876953890478582
48	21.32131167739982	28.53607479869603	28.468765934243105	21.673847589661044
49	21.090875487120872	28.619702949661885	27.81147226180068	22.477949301416565
50	20.97611616710425	27.824252425937313	28.048462382406104	23.151169024552335
51	21.418965239264335	26.769593957800552	28.873536671206356	22.937904131728757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18952.0
1	14323.0
2	9694.0
3	6105.0
4	2516.0
5	2344.0
6	2172.0
7	2368.5
8	2565.0
9	2829.5
10	3094.0
11	3519.5
12	3945.0
13	4147.5
14	4350.0
15	4767.0
16	5184.0
17	5530.5
18	5877.0
19	5560.5
20	5244.0
21	6046.0
22	6848.0
23	9530.5
24	12213.0
25	12086.5
26	13363.5
27	14767.0
28	20130.5
29	25494.0
30	28802.0
31	32110.0
32	35018.0
33	37926.0
34	44649.0
35	51372.0
36	56589.5
37	61807.0
38	63835.5
39	65864.0
40	70384.5
41	74905.0
42	86306.0
43	97707.0
44	102882.0
45	108057.0
46	113470.5
47	118884.0
48	113137.5
49	107391.0
50	105752.5
51	104114.0
52	96324.0
53	88534.0
54	82866.5
55	77199.0
56	73483.5
57	69768.0
58	65749.0
59	61730.0
60	57885.0
61	54040.0
62	50585.5
63	47131.0
64	42243.0
65	37355.0
66	32261.0
67	27167.0
68	23839.0
69	20511.0
70	18180.0
71	15849.0
72	13718.5
73	11588.0
74	10038.5
75	6832.5
76	5176.0
77	3904.0
78	2632.0
79	2179.5
80	1727.0
81	1190.0
82	653.0
83	615.0
84	577.0
85	507.0
86	437.0
87	284.5
88	132.0
89	89.5
90	47.0
91	35.5
92	24.0
93	19.0
94	14.0
95	10.0
96	6.0
97	3.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1525802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.218875487372436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.63414305619546	16.048714641035257
2	9.47475489146537	4.020872886307532
3	3.696079075146879	2.3527992506107287
4	1.8943123473081014	1.6078071132689122
5	1.2457889963059372	1.3217120798077178
6	0.910994934238713	1.1598172847542971
7	0.661253574113572	0.9821740078287119
8	0.5065515027745899	0.8598762612252332
9	0.42837777629433665	0.8180725227072311
>10	3.610880328923545	16.417577682839475
>50	0.9584191845386832	14.713441782002082
>100	0.9678056671010922	34.932880800666645
>500	0.005319332796861164	0.7048415783912424
>1k	0.004067725079952655	1.6921907788675206
>5k	9.38705787681382E-4	1.2806790886952408
>10k+	3.129019292271273E-4	1.086542240992152
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16365	1.0725506979280404	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGT	7231	0.47391470190758694	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCC	6246	0.4093584881917837	No Hit
AATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT	5812	0.38091443057487145	No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	4533	0.2970896616992244	Illumina Single End Adapter 1 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3640	0.23856306388378049	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2946	0.19307878741802673	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2752	0.1803641625846604	No Hit
AATGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT	1745	0.11436608419703213	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1572	0.10302778473222608	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.553930326477485E-5	0.0	0.0	0.3668234803729449	0.0
2	6.553930326477485E-5	0.0	0.0	0.4705721974410834	0.0
3	6.553930326477485E-5	0.0	0.0	0.6867863589115757	0.0
4	6.553930326477485E-5	0.0	0.0	1.599290078267036	0.0
5	6.553930326477485E-5	0.0	0.0	1.7139183196771273	0.0
6	6.553930326477485E-5	0.0	0.0	2.275262452139924	0.0
7	6.553930326477485E-5	0.0	0.0	2.7309572277399035	0.0
8	6.553930326477485E-5	0.0	0.0	3.0380744028386384	0.0
9	6.553930326477485E-5	0.0	0.0	4.10158067691614	0.0
10	6.553930326477485E-5	0.0	0.0	4.871798568883774	0.0
11	6.553930326477485E-5	0.0	0.0	6.1053793349333665	0.0
12	6.553930326477485E-5	0.0	0.0	6.471023107847545	0.0
13	6.553930326477485E-5	0.0	0.0	6.63978681375434	0.0
14	6.553930326477485E-5	0.0	0.0	6.885690279603776	0.0
15	6.553930326477485E-5	0.0	0.0	7.097120071935939	0.0
16	6.553930326477485E-5	0.0	0.0	7.401156899781229	0.0
17	6.553930326477485E-5	0.0	0.0	7.880118128040205	0.0
18	6.553930326477485E-5	0.0	0.0	8.409216923296732	0.0
19	6.553930326477485E-5	0.0	0.0	8.7082727640939	0.0
20	6.553930326477485E-5	0.0	0.0	8.944541952363412	0.0
21	6.553930326477485E-5	0.0	0.0	9.220331340501586	0.0
22	6.553930326477485E-5	0.0	0.0	9.52705527978073	0.0
23	6.553930326477485E-5	0.0	0.0	9.84931203393363	0.0
24	6.553930326477485E-5	0.0	0.0	10.082894110769287	0.0
25	6.553930326477485E-5	0.0	0.0	10.283772075275822	0.0
26	6.553930326477485E-5	0.0	0.0	10.493432306419837	0.0
27	6.553930326477485E-5	0.0	0.0	10.692671788344752	0.0
28	6.553930326477485E-5	0.0	0.0	10.906329916987918	0.0
29	6.553930326477485E-5	0.0	0.0	11.152888775870002	0.0
30	6.553930326477485E-5	0.0	0.0	11.430251107286528	0.0
31	6.553930326477485E-5	0.0	0.0	11.644236932446018	0.0
32	6.553930326477485E-5	0.0	0.0	11.875721751577203	0.0
33	6.553930326477485E-5	0.0	0.0	12.123525857221317	0.0
34	6.553930326477485E-5	0.0	0.0	12.352585722131705	0.0
35	6.553930326477485E-5	0.0	0.0	12.611990284453684	0.0
36	6.553930326477485E-5	0.0	0.0	12.839870441905306	0.0
37	6.553930326477485E-5	0.0	0.0	13.070044474971196	0.0
38	6.553930326477485E-5	0.0	0.0	13.324205893031992	0.0
39	6.553930326477485E-5	0.0	0.0	13.58098888322338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	20	7.034013E-4	45.000004	19
CGTATAG	40	6.8175723E-9	45.000004	1
CTATCGA	20	7.034013E-4	45.000004	1
TCGATTG	20	7.034013E-4	45.000004	41
TTGCGAC	20	7.034013E-4	45.000004	40
TACTCGC	65	0.0	45.000004	45
CCGAACG	20	7.034013E-4	45.000004	1
ATTGCGT	20	7.034013E-4	45.000004	14
CAATCGT	20	7.034013E-4	45.000004	24
TCGACAC	30	2.1659707E-6	45.000004	33
GTAACGA	20	7.034013E-4	45.000004	29
ACAACGT	25	3.891409E-5	45.0	34
GTCGAAC	35	1.2122837E-7	45.0	39
CGACATA	45	3.8562575E-10	45.0	25
ATCGTTA	35	1.2122837E-7	45.0	28
AATTGCG	35	1.2122837E-7	45.0	1
CGCTATA	35	1.2122837E-7	45.0	36
ACTCGAT	25	3.891409E-5	45.0	21
TCGTACC	25	3.891409E-5	45.0	42
CGAGACG	45	3.8562575E-10	45.0	1
>>END_MODULE