##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553311_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	761154
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.492176353274107	31.0	31.0	34.0	30.0	34.0
2	31.694416635792493	31.0	31.0	34.0	30.0	34.0
3	31.885956061454056	33.0	31.0	34.0	30.0	34.0
4	35.64872942926136	37.0	35.0	37.0	33.0	37.0
5	35.407464192528714	37.0	35.0	37.0	33.0	37.0
6	32.478505006871146	37.0	35.0	37.0	25.0	37.0
7	34.13878269049364	37.0	35.0	37.0	26.0	37.0
8	35.29697275452799	37.0	35.0	37.0	32.0	37.0
9	37.16253346891693	39.0	37.0	39.0	34.0	39.0
10	36.529346229540934	38.0	35.0	39.0	32.0	39.0
11	36.57579018175034	39.0	35.0	39.0	32.0	39.0
12	36.687429876214274	39.0	35.0	39.0	32.0	39.0
13	36.661252519201106	39.0	35.0	39.0	32.0	39.0
14	37.77882793757899	39.0	37.0	41.0	33.0	41.0
15	37.81019741077364	39.0	37.0	41.0	33.0	41.0
16	37.76104572793416	39.0	37.0	41.0	33.0	41.0
17	37.7229496264882	39.0	37.0	41.0	33.0	41.0
18	37.56812813175783	39.0	36.0	41.0	33.0	41.0
19	37.490083478507636	39.0	36.0	41.0	32.0	41.0
20	37.36939962215268	39.0	36.0	40.0	32.0	41.0
21	37.34295687863428	39.0	35.0	40.0	32.0	41.0
22	37.27440701881616	39.0	35.0	40.0	32.0	41.0
23	37.0782246431077	39.0	35.0	40.0	31.0	41.0
24	36.97404598806549	39.0	35.0	40.0	31.0	41.0
25	37.0032332484622	39.0	35.0	40.0	31.0	41.0
26	36.94345822264614	39.0	35.0	40.0	31.0	41.0
27	36.86656708103748	39.0	35.0	40.0	31.0	41.0
28	36.779353455411126	39.0	35.0	40.0	31.0	41.0
29	36.59712357814581	39.0	35.0	40.0	31.0	41.0
30	36.594305488771	39.0	35.0	40.0	31.0	41.0
31	36.216292629349645	38.0	35.0	40.0	30.0	41.0
32	36.326307159917704	38.0	35.0	40.0	30.0	41.0
33	36.30093253139312	38.0	35.0	40.0	30.0	41.0
34	36.29760863110487	38.0	35.0	40.0	30.0	41.0
35	36.26920307848346	38.0	35.0	40.0	30.0	41.0
36	36.21095074058601	38.0	35.0	40.0	30.0	41.0
37	36.09756369933023	38.0	35.0	40.0	30.0	41.0
38	35.995714402078946	38.0	35.0	40.0	30.0	41.0
39	35.921458732398435	38.0	35.0	40.0	30.0	41.0
40	35.8097191895464	38.0	35.0	40.0	29.0	41.0
41	35.801957291165785	38.0	35.0	40.0	29.0	41.0
42	35.681572979975144	38.0	35.0	40.0	28.0	41.0
43	35.623366624888	38.0	35.0	40.0	28.0	41.0
44	35.450907437916634	38.0	34.0	40.0	27.0	41.0
45	35.278064622927815	38.0	34.0	40.0	27.0	41.0
46	35.048203123152476	38.0	34.0	40.0	26.0	41.0
47	35.03450550085791	38.0	34.0	40.0	26.0	41.0
48	35.02628377437417	37.0	34.0	40.0	26.0	41.0
49	34.877735648764904	37.0	33.0	40.0	26.0	41.0
50	34.77061278006816	37.0	33.0	40.0	26.0	41.0
51	33.68451456604051	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	3.0
10	7.0
11	10.0
12	2.0
13	7.0
14	18.0
15	34.0
16	82.0
17	212.0
18	414.0
19	771.0
20	1287.0
21	1992.0
22	2958.0
23	3858.0
24	4560.0
25	5491.0
26	6796.0
27	8042.0
28	10138.0
29	13024.0
30	16658.0
31	22030.0
32	28589.0
33	38425.0
34	53600.0
35	77663.0
36	82643.0
37	107155.0
38	139150.0
39	135503.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.925139984812535	33.019730567007464	21.557135612504172	25.49799383567583
2	23.27150616038279	33.926642965812434	19.901623061824544	22.90022781198023
3	20.38260325768504	36.10701645133573	18.67453892379203	24.835841367187193
4	18.34293717171558	37.71812274520005	18.28368503614249	25.655255046941882
5	16.870830344450663	37.986793736878475	16.22825341520901	28.914122503461847
6	16.746808136067077	46.42293149612299	17.034003631328222	19.79625673648171
7	82.6111404525234	10.241685650998352	3.795815301502718	3.351358594975524
8	84.10374247524153	9.489012735924662	3.1368159400068847	3.270428848826913
9	77.49154573187555	13.65584362691387	5.788447541496202	3.064163099714381
10	29.750615512760888	46.8128657275663	11.04204930933819	12.394469450334624
11	18.35515546131269	33.532635971170095	31.0789932129372	17.03321535458002
12	23.235508188881617	28.123743683932556	31.174111940553423	17.466636186632403
13	22.351981333606602	30.387148986933	28.47754856441666	18.783321115043737
14	19.003907225081914	34.01453582323683	27.280813081189876	19.700743870491387
15	16.85585308623485	35.61040209996926	28.43222265139512	19.101522162400776
16	19.79967260239058	33.22231769129506	27.197912643170763	19.780097063143597
17	18.543290845216607	33.48875523218692	28.394122608565414	19.57383131403106
18	18.63459956854986	33.16543038596657	27.990262154570562	20.20970789091301
19	18.800794582962187	36.721740935474294	27.291848955664687	17.185615525898832
20	20.49716614509022	35.10918946757161	26.44944387075415	17.94420051658403
21	20.35895495523902	34.7883608310539	26.55704364688355	18.295640566823536
22	18.72919277833395	32.60049871642269	26.244754675138015	22.42555383010534
23	18.365665817955367	36.2753135370766	25.686523357953845	19.672497287014192
24	16.26004724405311	35.35710250488074	28.11480988078628	20.268040370279863
25	18.794225610060515	35.55535410705324	24.920975255992875	20.729445026893377
26	19.888222357105132	34.693636241811774	24.13256712833408	21.28557427274901
27	17.438126844239143	33.907592944397585	26.286533342792655	22.367746868570617
28	17.033083975121986	35.98168044837182	26.968918247818447	20.016317328687755
29	18.77399317352336	34.598911652569655	25.18071244452502	21.446382729381966
30	20.11485192221285	33.20760319199531	26.053466184241298	20.62407870155054
31	19.88743408035693	35.17974023653558	22.805765981654172	22.12705970145332
32	18.695165498703282	33.2358497754725	25.006634662630688	23.062350063193517
33	18.501774936478032	34.70007383525541	25.270838752735976	21.52731247553058
34	17.038339153443324	33.80301489580295	25.408918563129145	23.74972738762458
35	17.522866594670724	33.91757778320813	26.41948935432252	22.140066267798634
36	18.644321648444336	34.26428817295843	26.35931756254319	20.732072616054044
37	17.781027229706474	36.52427761005001	24.538266894741405	21.156428265502118
38	18.67375064704383	34.425490767965485	26.48754391358385	20.413214671406838
39	19.536650927407596	32.52245931835082	24.715235024712477	23.22565472952911
40	19.39068834953242	33.55273702824921	25.241935271968618	21.814639350249752
41	17.42104751469479	32.18639066470123	27.17728606825951	23.215275752344468
42	19.36717142654443	33.186976617084056	25.017407778189433	22.428444178182076
43	18.107373803461584	33.66913922806686	26.824006705607538	21.39948026286402
44	19.281643399364647	31.602934491574636	26.85947915927657	22.255942949784142
45	19.617974811930306	33.075698216129716	26.513819805190543	20.792507166749434
46	18.16307869366777	32.68313639552574	27.58180341954453	21.57198149126195
47	18.692012391710485	34.29030130564905	25.988696111430805	21.02899019120966
48	18.510314601250208	33.06860372539591	26.611697501425468	21.809384171928414
49	18.541320153346103	33.02354057129043	26.700772773972155	21.734366501391307
50	17.316338086642126	31.101590479718954	27.819337479669027	23.762733953969892
51	18.554589478607483	30.67276792869774	27.94519900046508	22.82744359222969
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	25497.0
1	18969.5
2	12442.0
3	7733.5
4	3025.0
5	2463.5
6	1902.0
7	2127.5
8	2353.0
9	2596.0
10	2839.0
11	3378.5
12	3918.0
13	4109.0
14	4300.0
15	5027.5
16	5755.0
17	5242.0
18	4729.0
19	4599.5
20	4470.0
21	4872.5
22	5275.0
23	6151.0
24	7027.0
25	7508.0
26	10378.5
27	12768.0
28	14635.5
29	16503.0
30	16658.5
31	16814.0
32	19388.0
33	21962.0
34	24934.5
35	27907.0
36	30306.0
37	32705.0
38	32517.0
39	32329.0
40	35002.5
41	37676.0
42	37416.0
43	37156.0
44	38993.5
45	40831.0
46	45688.0
47	50545.0
48	56622.5
49	62700.0
50	65661.0
51	68622.0
52	62998.0
53	57374.0
54	50663.0
55	43952.0
56	37310.0
57	30668.0
58	26658.5
59	22649.0
60	20873.5
61	19098.0
62	15419.5
63	11741.0
64	10122.5
65	8504.0
66	7108.5
67	5713.0
68	4442.0
69	3171.0
70	2824.0
71	2477.0
72	2271.5
73	2066.0
74	1740.0
75	1275.5
76	1137.0
77	769.5
78	402.0
79	304.0
80	206.0
81	279.0
82	352.0
83	219.0
84	86.0
85	73.5
86	61.0
87	43.5
88	26.0
89	17.0
90	8.0
91	6.0
92	4.0
93	3.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	761154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.301425229037545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.98686019397432	18.97274436358133
2	10.078772183121156	5.100146015834868
3	3.8799158731866354	2.9450220408116268
4	2.085366437944159	2.1105097201915406
5	1.2998951411212343	1.6444599859334057
6	0.9223131093275235	1.400150170404688
7	0.6948704184190522	1.2306848354849778
8	0.544520337248355	1.102171247886365
9	0.45334754668122257	1.0323305149610305
>10	3.7310677964726193	19.828782261762434
>50	0.7449178858470242	13.412194523014554
>100	0.5686837814976037	24.948425471301498
>500	0.005786791486099575	0.9108286321737347
>1k	0.002630359766408898	1.6902839105617795
>5k	5.260719532817796E-4	0.6757674945997444
>10k+	5.260719532817796E-4	2.9954988114964056
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22505	2.956694703043011	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5077	0.667013508435875	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3969	0.5214450689348016	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	3639	0.4780898477837599	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2383	0.31307724849373453	No Hit
AAATCAAGGGAGAATTTTTTTCTTGTTCTGAGTATCCTTGACATTACAATT	1458	0.19155124981278426	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1250	0.16422432254182465	No Hit
AACACTGGGGTCACTCCCTGGAATTCACCATCCCACGTTTCTTCACTAACA	977	0.12835773049869015	No Hit
TCTACCGGGTGGTAATGTGGCCAGCTCTCCTTGGTCCTGTCCTCAGCCGAT	785	0.1031328745562659	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.567559258704546	0.0
2	0.0	0.0	0.0	0.7462353216300512	0.0
3	0.0	0.0	0.0	0.9471145129632111	0.0
4	0.0	0.0	0.0	1.8482462156147113	0.0
5	0.0	0.0	0.0	1.9751587720750334	0.0
6	0.0	0.0	0.0	2.740312735661903	0.0
7	0.0	0.0	0.0	3.38499173623209	0.0
8	0.0	0.0	0.0	3.9463761604090632	0.0
9	0.0	0.0	0.0	4.839887854494623	0.0
10	0.0	0.0	0.0	6.117947222244119	0.0
11	0.0	0.0	0.0	7.298260273216721	0.0
12	0.0	0.0	0.0	7.883292999839717	0.0
13	0.0	0.0	0.0	8.113075671940239	0.0
14	0.0	0.0	0.0	8.346931107239797	0.0
15	0.0	0.0	0.0	8.56239341841467	0.0
16	0.0	0.0	0.0	9.022747039363914	0.0
17	0.0	0.0	0.0	9.65980603136816	0.0
18	0.0	0.0	0.0	10.463716935074899	0.0
19	0.0	0.0	0.0	10.797814896853987	0.0
20	0.0	0.0	0.0	11.182756708892024	0.0
21	0.0	0.0	0.0	11.573216458167467	0.0
22	0.0	0.0	0.0	12.025555932176669	0.0
23	0.0	0.0	0.0	12.517834761428043	0.0
24	0.0	0.0	0.0	12.842867540602821	0.0
25	0.0	0.0	0.0	13.124019580794425	0.0
26	0.0	0.0	0.0	13.378764349921303	0.0
27	0.0	0.0	0.0	13.686455040635666	0.0
28	0.0	0.0	0.0	13.98586882549392	0.0
29	0.0	0.0	0.0	14.304464011225061	0.0
30	0.0	0.0	0.0	14.698733764783473	0.0
31	0.0	0.0	0.0	14.979491666600977	0.0
32	0.0	0.0	0.0	15.289415808101909	0.0
33	1.3137945803345973E-4	0.0	0.0	15.61037582407765	0.0
34	1.3137945803345973E-4	0.0	0.0	15.92778859468649	0.0
35	1.3137945803345973E-4	0.0	0.0	16.26924380611545	0.0
36	1.3137945803345973E-4	0.0	0.0	16.57640897899768	0.0
37	1.3137945803345973E-4	0.0	0.0	16.897500374431456	0.0
38	2.6275891606691946E-4	0.0	0.0	17.24289696960142	0.0
39	2.6275891606691946E-4	0.0	0.0	17.588293564771387	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATG	75	0.0	45.000004	36
CGCGACG	35	1.2112105E-7	45.000004	1
TACATCG	55	1.8189894E-12	45.000004	2
TCACTAG	55	1.8189894E-12	45.000004	1
TTACGCG	35	1.2112105E-7	45.000004	1
ACGTAAG	35	1.2112105E-7	45.000004	1
TCGAAAG	35	1.2112105E-7	45.000004	1
TATTTCG	20	7.031784E-4	45.0	39
TCGTTAC	25	3.8895603E-5	45.0	15
CGAACCG	25	3.8895603E-5	45.0	1
TCACCGG	50	2.1827873E-11	45.0	2
CTCGTCA	45	3.8380676E-10	45.0	36
GTCGAGA	20	7.031784E-4	45.0	25
CTCCGCT	20	7.031784E-4	45.0	17
CGAGTCG	25	3.8895603E-5	45.0	1
CGAAACG	25	3.8895603E-5	45.0	1
ACGCAGT	20	7.031784E-4	45.0	29
TCTTCGC	25	3.8895603E-5	45.0	22
CATGACG	25	3.8895603E-5	45.0	1
GCTATCG	20	7.031784E-4	45.0	1
>>END_MODULE