FastQCFastQC Report
Sat 18 Jun 2016
SRR3553310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352696
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136841.01160940817449No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT35320.26110818690969734Illumina Single End Adapter 1 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31990.23649068231147277No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24640.18215474873881493No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG16030.11850408369655858No Hit
AACTTAGGAGATCTTGACAAATTCATTCTTGTTTCCCCTTCCTAGTTCCAA14240.10527125089450994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG302.165787E-645.0000041
TCGCAAT351.2121382E-745.00000429
GTCGATT253.891172E-545.00000418
CGTATCG253.891172E-545.0000041
TACTTCG253.891172E-545.0000041
CGAAACG253.891172E-545.0000041
CTATCGA351.2121382E-745.00000420
TTCGTTG253.891172E-545.00000426
ATTCGCG253.891172E-545.0000041
TTCCGTA302.165787E-645.00000429
TCTAGCG551.8189894E-1245.0000041
TAGCCGA302.165787E-645.00000428
ATCGTAG302.165787E-645.0000041
TTATACG551.8189894E-1245.0000041
ACGATAG302.165787E-645.0000041
AGATTAC351.2121382E-745.00000421
CGTACTC253.891172E-545.00000445
CGTCGTT253.891172E-545.00000425
CGGTCGA253.891172E-545.00000412
GCTACGA700.045.0000049