##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553310_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1352696 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.756236434498216 31.0 31.0 34.0 30.0 34.0 2 31.95239580807513 33.0 31.0 34.0 30.0 34.0 3 32.12677645235885 34.0 31.0 34.0 30.0 34.0 4 35.83062195792699 37.0 35.0 37.0 35.0 37.0 5 35.58217441317192 37.0 35.0 37.0 33.0 37.0 6 34.19288517153891 37.0 35.0 37.0 32.0 37.0 7 35.02641317783153 37.0 35.0 37.0 32.0 37.0 8 35.71908618048697 37.0 35.0 37.0 33.0 37.0 9 37.42010843530254 39.0 37.0 39.0 35.0 39.0 10 36.85038397393058 39.0 37.0 39.0 32.0 39.0 11 36.84134203102545 39.0 37.0 39.0 32.0 39.0 12 36.72037989319108 39.0 35.0 39.0 32.0 39.0 13 36.700831524599764 39.0 35.0 39.0 32.0 39.0 14 37.890302773128624 40.0 37.0 41.0 33.0 41.0 15 37.89722968057864 40.0 37.0 41.0 33.0 41.0 16 37.80512694648317 40.0 37.0 41.0 33.0 41.0 17 37.801665710551376 39.0 37.0 41.0 33.0 41.0 18 37.714011130364845 39.0 36.0 41.0 33.0 41.0 19 37.728953881729524 39.0 36.0 41.0 33.0 41.0 20 37.621612690508435 39.0 36.0 41.0 33.0 41.0 21 37.58129099221111 39.0 36.0 41.0 32.0 41.0 22 37.540558262906075 39.0 36.0 41.0 32.0 41.0 23 37.41121434527788 39.0 36.0 41.0 32.0 41.0 24 37.21618678550095 39.0 35.0 41.0 32.0 41.0 25 37.28095595758396 39.0 35.0 41.0 32.0 41.0 26 37.22084193344255 39.0 35.0 41.0 32.0 41.0 27 37.121112947772446 39.0 35.0 41.0 32.0 41.0 28 37.046798393726306 39.0 35.0 40.0 31.0 41.0 29 36.88929885207023 39.0 35.0 40.0 31.0 41.0 30 36.94824335992714 39.0 35.0 40.0 31.0 41.0 31 36.86636465251616 39.0 35.0 40.0 31.0 41.0 32 36.77291571794402 39.0 35.0 40.0 31.0 41.0 33 36.74798920082561 39.0 35.0 40.0 31.0 41.0 34 36.65257678000083 39.0 35.0 40.0 31.0 41.0 35 36.54129974510163 39.0 35.0 40.0 30.0 41.0 36 36.48566270618084 39.0 35.0 40.0 30.0 41.0 37 36.409495555542414 38.0 35.0 40.0 30.0 41.0 38 36.32730340002484 38.0 35.0 40.0 30.0 41.0 39 36.27632890168966 38.0 35.0 40.0 30.0 41.0 40 36.1559049483402 38.0 35.0 40.0 30.0 41.0 41 36.01619580452667 38.0 35.0 40.0 30.0 41.0 42 35.92859223358389 38.0 35.0 40.0 29.0 41.0 43 35.84383113426816 38.0 35.0 40.0 29.0 41.0 44 35.70804452737348 38.0 34.0 40.0 29.0 41.0 45 35.532926097216226 38.0 34.0 40.0 28.0 41.0 46 35.39357253958022 38.0 34.0 40.0 28.0 41.0 47 35.29316934477517 37.0 34.0 40.0 28.0 41.0 48 35.16396219106141 37.0 34.0 40.0 27.0 41.0 49 34.990393259091476 37.0 34.0 40.0 27.0 41.0 50 34.91269139555377 37.0 34.0 40.0 27.0 41.0 51 33.675496194266856 36.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 6.0 10 13.0 11 10.0 12 19.0 13 34.0 14 59.0 15 102.0 16 182.0 17 342.0 18 597.0 19 1053.0 20 1755.0 21 2719.0 22 3696.0 23 5071.0 24 6588.0 25 8189.0 26 10377.0 27 12727.0 28 16135.0 29 20735.0 30 27095.0 31 35404.0 32 46799.0 33 64097.0 34 95909.0 35 119988.0 36 138064.0 37 190679.0 38 269624.0 39 274587.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.333032699143043 30.937771679667865 18.58865554418731 27.14054007700178 2 24.945072654905463 28.97835138124161 19.66199352995795 26.414582433894978 3 21.416563662493274 31.27835078983009 19.442136296699335 27.86294925097731 4 21.62799328156511 32.272365705228665 18.546665326133883 27.552975687072333 5 18.872828780450302 34.351916469036645 16.952959127549722 29.822295622963324 6 19.34159633797986 41.070277431144916 17.886723994156853 21.701402236718376 7 86.34216409304086 6.146835652652185 3.9076777043770368 3.603322549929918 8 87.58841602252096 5.620627251060105 3.6342238019481097 3.156732924470834 9 80.20338642237428 9.300315813752684 5.905317972404738 4.590979791468297 10 36.91221087369224 33.25824871220141 13.198309154458949 16.6312312596474 11 28.63621981583445 30.134856612276522 22.718038642828837 18.510884929060186 12 27.563768947346635 26.264955318859524 27.548170468457066 18.62310526533678 13 25.725661937345862 26.361281470485608 26.829014057851875 21.084042534316655 14 21.898194420623703 28.4023165589312 27.637621461141304 22.061867559303792 15 21.74073110292335 29.909602748880754 27.435802279300002 20.913863868895895 16 23.24439489730139 28.366018676775862 27.578258529632677 20.811327896290074 17 23.400379686197045 28.11363380981388 26.88933803308356 21.59664847090551 18 23.46040795566779 28.40823067414999 26.236789345130024 21.89457202505219 19 23.266203197170686 30.064035082531475 25.61536368851538 21.054398031782455 20 25.480004376445265 28.440166896331476 25.280107282050068 20.799721445173194 21 25.70991560557583 28.80684203989662 25.171805047105927 20.311437307421624 22 24.115248363268613 27.325577956909758 25.115325246766457 23.443848433055173 23 22.889547984173827 29.742233288188917 25.605901104165312 21.76231762347194 24 21.60758958406028 28.508548853548767 27.36572001395731 22.518141548433647 25 23.657791551095 29.506925428921203 24.38611484028932 22.449168179694475 26 24.62075736159492 28.759455191706046 24.178972954751103 22.440814491947933 27 21.95947943957844 29.18822854506852 26.231984126514753 22.62030788883829 28 21.31713260037732 30.634525421824264 27.131742830613824 20.916599147184588 29 22.09350807572433 31.694482721912387 24.519773844234034 21.692235358129246 30 22.767495431345992 28.466484709055102 26.11185366113303 22.654166198465877 31 24.879130270215924 28.74289566909343 24.547865891523298 21.83010816916735 32 22.318540159799394 29.34310443736065 26.274565756090063 22.0637896467499 33 21.837722592511547 28.28787842944756 26.50861686587378 23.36578211216711 34 21.3130666461644 29.645463577921426 26.87425703927564 22.167212736638536 35 22.331033728199092 28.159616055639997 26.951658022201592 22.557692193959323 36 22.801058035212645 31.003270505715992 24.56132050364605 21.63435095542531 37 21.566042924648258 30.91463270387434 24.5917042705826 22.927620100894806 38 21.691643946607368 30.4624246689574 25.732611022727948 22.113320361707288 39 22.097500103497016 28.896514811901564 25.311599945590142 23.694385139011278 40 23.961185661819062 27.531241313643275 26.403123835658565 22.104449188879098 41 21.13505177807874 27.46892132452524 29.412890996942405 21.98313590045361 42 22.141486335436785 28.80876412734273 25.6679253875224 23.381824149698087 43 21.805934223210535 28.58003572125592 26.35862011863715 23.255409936896392 44 22.756110759549816 27.26776748064606 26.27323508016583 23.702886679638294 45 23.525241443753806 27.6545506159551 26.508912571634717 22.311295368656374 46 21.8043817679656 28.45303009693235 27.366681057680363 22.375907077421683 47 22.594433634756072 29.477206999946777 26.248691502007844 21.67966786328931 48 21.60914203930521 28.328759750897465 27.36978596817023 22.692312241627093 49 21.424178085837468 28.73454198134688 27.326465074192573 22.514814858623076 50 21.132390426230284 27.451770390390745 27.777933844707164 23.637905338671807 51 21.197593546517474 27.59548339020741 27.201159757994404 24.005763305280713 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15877.0 1 12329.5 2 8782.0 3 5616.5 4 2451.0 5 2227.5 6 2004.0 7 2038.0 8 2072.0 9 2372.0 10 2672.0 11 3104.5 12 3537.0 13 3522.5 14 3508.0 15 3886.5 16 4265.0 17 4890.0 18 5515.0 19 4868.0 20 4221.0 21 4738.0 22 5255.0 23 6414.0 24 7573.0 25 8848.5 26 11352.5 27 12581.0 28 16488.0 29 20395.0 30 24508.5 31 28622.0 32 30305.5 33 31989.0 34 37407.0 35 42825.0 36 46073.0 37 49321.0 38 51347.0 39 53373.0 40 55931.0 41 58489.0 42 62763.5 43 67038.0 44 72881.0 45 78724.0 46 89608.5 47 100493.0 48 98252.5 49 96012.0 50 93499.5 51 90987.0 52 87722.5 53 84458.0 54 81141.0 55 77824.0 56 72603.0 57 67382.0 58 63842.5 59 60303.0 60 60008.0 61 59713.0 62 54393.0 63 49073.0 64 44135.0 65 39197.0 66 34809.5 67 30422.0 68 25244.0 69 20066.0 70 19414.0 71 18762.0 72 17315.5 73 15869.0 74 12467.0 75 7549.5 76 6034.0 77 4478.5 78 2923.0 79 2188.0 80 1453.0 81 1144.0 82 835.0 83 560.5 84 286.0 85 247.5 86 209.0 87 136.5 88 64.0 89 44.0 90 24.0 91 20.5 92 17.0 93 12.0 94 7.0 95 4.5 96 2.0 97 2.5 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1352696.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.31791154913889 #Duplication Level Percentage of deduplicated Percentage of total 1 76.61613511972197 15.56679856599385 2 9.533001784216532 3.8738137409898954 3 3.4126011712629807 2.0801078625062708 4 1.672260569697424 1.359073693688995 5 1.0259023081741205 1.0422096177769604 6 0.7105463360478527 0.8662090564430994 7 0.48427702390315686 0.6887648415861193 8 0.39931483488558706 0.6490594796371487 9 0.33875487496061385 0.6194512427660422 >10 3.4502688547132108 16.369656493800942 >50 1.2629385600486729 18.741568451243072 >100 1.0850282974219436 35.24631114788058 >500 0.006353937670056161 0.8453689490643946 >1k 0.0022425662364904102 1.0124379113300852 >5k 0.0 0.0 >10k+ 3.7376103941506834E-4 1.0391689452925958 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13684 1.01160940817449 No Hit CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT 3532 0.26110818690969734 Illumina Single End Adapter 1 (95% over 21bp) CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3199 0.23649068231147277 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2464 0.18215474873881493 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 1603 0.11850408369655858 No Hit AACTTAGGAGATCTTGACAAATTCATTCTTGTTTCCCCTTCCTAGTTCCAA 1424 0.10527125089450994 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.478528804698173E-4 0.0 0.0 0.30332018428383023 0.0 2 1.478528804698173E-4 0.0 0.0 0.4088132144990449 0.0 3 1.478528804698173E-4 0.0 0.0 0.5178547138455352 0.0 4 1.478528804698173E-4 0.0 0.0 0.9191274314406194 0.0 5 1.478528804698173E-4 0.0 0.0 0.9903185933868364 0.0 6 1.478528804698173E-4 0.0 0.0 1.4327683381927647 0.0 7 1.478528804698173E-4 0.0 0.0 1.7429636814184415 0.0 8 1.478528804698173E-4 0.0 0.0 2.0107991743895153 0.0 9 1.478528804698173E-4 0.0 0.0 2.4244915339440642 0.0 10 1.478528804698173E-4 0.0 0.0 3.140912666260564 0.0 11 1.478528804698173E-4 0.0 0.0 3.7450395358602377 0.0 12 1.478528804698173E-4 0.0 0.0 4.047250823540544 0.0 13 1.478528804698173E-4 0.0 0.0 4.175291418027406 0.0 14 1.478528804698173E-4 0.0 0.0 4.318191227001485 0.0 15 1.478528804698173E-4 0.0 0.0 4.460647477334153 0.0 16 1.478528804698173E-4 0.0 0.0 4.744229302075263 0.0 17 1.478528804698173E-4 0.0 0.0 5.121993411675646 0.0 18 1.478528804698173E-4 0.0 0.0 5.543965532536505 0.0 19 1.478528804698173E-4 0.0 0.0 5.7502203007919 0.0 20 1.478528804698173E-4 0.0 0.0 5.974808826225552 0.0 21 1.478528804698173E-4 0.0 0.0 6.240870084630989 0.0 22 1.478528804698173E-4 0.0 0.0 6.523860497850219 0.0 23 1.478528804698173E-4 0.0 0.0 6.819492332349619 0.0 24 1.478528804698173E-4 0.0 0.0 7.029443422616759 0.0 25 1.478528804698173E-4 0.0 0.0 7.225496342119738 0.0 26 1.478528804698173E-4 0.0 0.0 7.402624092922578 0.0 27 1.478528804698173E-4 0.0 0.0 7.588770869434079 0.0 28 1.478528804698173E-4 0.0 0.0 7.778244335756149 0.0 29 1.478528804698173E-4 0.0 0.0 7.9820595314837925 0.0 30 1.478528804698173E-4 0.0 0.0 8.229417400509798 0.0 31 1.478528804698173E-4 0.0 0.0 8.432862964036266 0.0 32 3.696322011745433E-4 0.0 0.0 8.63970914381354 0.0 33 3.696322011745433E-4 0.0 0.0 8.854539379136185 0.0 34 3.696322011745433E-4 0.0 0.0 9.071439554785407 0.0 35 3.696322011745433E-4 0.0 0.0 9.312365823510973 0.0 36 3.696322011745433E-4 0.0 0.0 9.511449727063583 0.0 37 3.696322011745433E-4 0.0 0.0 9.719109097683441 0.0 38 3.696322011745433E-4 0.0 0.0 9.957743646761726 0.0 39 4.4355864140945196E-4 0.0 0.0 10.206727897472899 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCCG 30 2.165787E-6 45.000004 1 TCGCAAT 35 1.2121382E-7 45.000004 29 GTCGATT 25 3.891172E-5 45.000004 18 CGTATCG 25 3.891172E-5 45.000004 1 TACTTCG 25 3.891172E-5 45.000004 1 CGAAACG 25 3.891172E-5 45.000004 1 CTATCGA 35 1.2121382E-7 45.000004 20 TTCGTTG 25 3.891172E-5 45.000004 26 ATTCGCG 25 3.891172E-5 45.000004 1 TTCCGTA 30 2.165787E-6 45.000004 29 TCTAGCG 55 1.8189894E-12 45.000004 1 TAGCCGA 30 2.165787E-6 45.000004 28 ATCGTAG 30 2.165787E-6 45.000004 1 TTATACG 55 1.8189894E-12 45.000004 1 ACGATAG 30 2.165787E-6 45.000004 1 AGATTAC 35 1.2121382E-7 45.000004 21 CGTACTC 25 3.891172E-5 45.000004 45 CGTCGTT 25 3.891172E-5 45.000004 25 CGGTCGA 25 3.891172E-5 45.000004 12 GCTACGA 70 0.0 45.000004 9 >>END_MODULE