FastQCFastQC Report
Sat 18 Jun 2016
SRR3553305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606816
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA138630.8627621333120904No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT30480.18969191245295042Illumina Single End Adapter 2 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28420.1768715272937287No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27370.17033686495529046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT351.2123382E-745.00000420
CTCCGAT253.891502E-545.011
TAGGTCG406.8175723E-945.02
ACCGAAC406.8175723E-945.025
CGCGAAA207.034125E-445.020
CCGTCGA207.034125E-445.030
AATTCGC207.034125E-445.043
TCGTACA253.891502E-545.034
CGCACGA207.034125E-445.040
CGATACG207.034125E-445.01
GTGCGAT302.1660435E-644.99999615
ACGAACG850.042.352941
CTCACGA800.042.187524
TATACGG1450.041.896552
ATAACGG1800.041.252
TACGGGT2900.041.120694
CGAACCG556.184564E-1140.90909227
CGTAGAT556.184564E-1140.90909221
TCAACCG1100.040.9090921
TTCTACG451.9290383E-840.01