##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1606816
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65526793360285	31.0	31.0	34.0	30.0	34.0
2	31.86036733515225	33.0	31.0	34.0	30.0	34.0
3	32.036821888753906	33.0	31.0	34.0	30.0	34.0
4	35.75906886662816	37.0	35.0	37.0	35.0	37.0
5	35.530752743313485	37.0	35.0	37.0	33.0	37.0
6	32.73653735088523	37.0	35.0	37.0	26.0	37.0
7	34.295859637942364	37.0	35.0	37.0	28.0	37.0
8	35.42419107103738	37.0	35.0	37.0	32.0	37.0
9	37.31324432915779	39.0	37.0	39.0	34.0	39.0
10	36.71522688347639	39.0	37.0	39.0	32.0	39.0
11	36.739953423416246	39.0	37.0	39.0	32.0	39.0
12	36.74931728337283	39.0	35.0	39.0	32.0	39.0
13	36.74107863003605	39.0	37.0	39.0	33.0	39.0
14	37.89260686973493	40.0	37.0	41.0	33.0	41.0
15	37.928172236273475	40.0	37.0	41.0	33.0	41.0
16	37.890819483998165	40.0	37.0	41.0	33.0	41.0
17	37.8810510973254	40.0	37.0	41.0	33.0	41.0
18	37.803951416963734	39.0	37.0	41.0	33.0	41.0
19	37.82920197458825	40.0	37.0	41.0	33.0	41.0
20	37.73755613586123	39.0	37.0	41.0	33.0	41.0
21	37.693236811184356	39.0	36.0	41.0	33.0	41.0
22	37.62726410491307	39.0	36.0	41.0	33.0	41.0
23	37.502815505944675	39.0	36.0	41.0	32.0	41.0
24	37.375125714456416	39.0	36.0	41.0	32.0	41.0
25	37.41343252743314	39.0	36.0	41.0	32.0	41.0
26	37.348722566865156	39.0	36.0	41.0	32.0	41.0
27	37.29433363869914	39.0	36.0	41.0	32.0	41.0
28	37.23808388763866	39.0	36.0	41.0	32.0	41.0
29	37.0419077230996	39.0	35.0	40.0	31.0	41.0
30	37.10141111365583	39.0	35.0	40.0	31.0	41.0
31	36.717832657877445	39.0	35.0	40.0	30.0	41.0
32	36.827117728476686	39.0	35.0	40.0	31.0	41.0
33	36.85129162268735	39.0	35.0	40.0	31.0	41.0
34	36.803457894369984	39.0	35.0	40.0	31.0	41.0
35	36.79799553900384	39.0	35.0	40.0	31.0	41.0
36	36.75512068587816	39.0	35.0	40.0	31.0	41.0
37	36.698154611355626	39.0	35.0	40.0	31.0	41.0
38	36.598683981239915	39.0	35.0	40.0	30.0	41.0
39	36.55930299424452	39.0	35.0	40.0	30.0	41.0
40	36.43978837651604	39.0	35.0	40.0	30.0	41.0
41	36.40999405034553	39.0	35.0	40.0	30.0	41.0
42	36.297054547627106	38.0	35.0	40.0	30.0	41.0
43	36.23974244717503	38.0	35.0	40.0	30.0	41.0
44	36.08897720709776	38.0	35.0	40.0	30.0	41.0
45	35.91696373449107	38.0	35.0	40.0	29.0	41.0
46	35.65489763607034	38.0	34.0	40.0	28.0	41.0
47	35.61076999482206	38.0	34.0	40.0	28.0	41.0
48	35.60951222790911	38.0	34.0	40.0	28.0	41.0
49	35.45194907195348	38.0	34.0	40.0	28.0	41.0
50	35.33015665763846	37.0	34.0	40.0	28.0	41.0
51	34.235727052755266	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	14.0
11	22.0
12	22.0
13	38.0
14	63.0
15	109.0
16	165.0
17	345.0
18	570.0
19	1033.0
20	1701.0
21	2683.0
22	3836.0
23	5165.0
24	6913.0
25	8735.0
26	10930.0
27	13999.0
28	18324.0
29	24127.0
30	31436.0
31	41165.0
32	55049.0
33	74748.0
34	107596.0
35	139637.0
36	169589.0
37	235206.0
38	324126.0
39	329412.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.847803357696215	31.961033497301493	19.32106725350009	26.8700958915022
2	23.516756118933344	30.175639276681338	19.798968892517873	26.508635711867445
3	22.27305428873001	30.741665504948916	20.105164499233265	26.880115707087803
4	21.019643817338142	31.482820683886644	19.423692569653277	28.073842929121938
5	18.651108776611636	34.650513811164444	16.58167456634736	30.116702845876564
6	18.411940135024796	42.11222691334913	17.693749626590723	21.78208332503535
7	87.18005048493418	5.726729133889631	3.8494139963754406	3.243806384800749
8	88.57753470216876	4.897947244737419	3.2160496285822395	3.3084684245115805
9	81.79368390655807	8.041617708561528	5.843730707187382	4.320967677693028
10	35.34337472367714	36.43808625256408	13.252170752593951	14.966368271164837
11	24.74135184115667	29.382020094397866	26.90575647740625	18.970871587039213
12	25.610835341445444	25.50229771174795	29.3208432079342	19.566023738872403
13	24.769793181048733	25.690869396371458	28.29695497182005	21.242382450759763
14	21.484351661920222	28.43536534363611	28.78344502419692	21.29683797024675
15	19.99955190886822	30.943306514249297	28.537430545874575	20.519711031007905
16	22.951663413856966	28.37499751060482	27.676722163583133	20.996616911955073
17	21.90630414434509	28.59574462788521	27.408365363551273	22.08958586421843
18	21.943084958078586	28.8533347937785	27.788869416286623	21.414710831856294
19	22.069483998167804	30.439577400274832	27.393055583215503	20.097883018341864
20	23.831975783163724	28.742556708422125	27.01516539541553	20.410302112998625
21	24.7201297472766	28.265837532113196	27.17050365443212	19.843529066178082
22	22.733094517356065	27.186995897476752	27.550074183976257	22.529835401190926
23	22.01957162487802	29.642286360105945	26.46793410073089	21.870207914285146
24	20.712079043275647	29.61135562503734	28.232479636747453	21.444085694939556
25	22.755996952980304	29.417245036146017	25.58438551769462	22.242372493179058
26	22.74516808396232	29.403242188277932	25.13772578814251	22.71386393961723
27	21.429709437794994	28.542720510624736	27.323103578754505	22.704466472825764
28	21.632035030768925	29.458755700714956	28.780333280226234	20.128875988289884
29	21.96418258219983	29.93342112600323	26.499113775317152	21.603282516479798
30	23.076506581960846	28.631405213789257	26.920817318224366	21.37127088602553
31	23.271052815008066	30.05073387369805	24.702081632246628	21.97613167904726
32	23.159652380857544	29.4812224921833	26.323798120008764	21.035327006950393
33	22.103588712086513	29.24728158046721	26.848687092983887	21.80044261446239
34	20.677351980562804	29.282444287335952	27.838408380299924	22.201795351801326
35	21.683503276044053	28.52461016071535	28.07608338478083	21.715803178459762
36	23.140421803118713	29.479977794595026	27.44184772867584	19.93775267361042
37	21.85159968534045	29.879712425069204	26.388460159719596	21.88022772987075
38	21.32328779399757	29.543208432079343	28.038929161770852	21.094574612152233
39	21.787248820026687	27.83822167566168	27.770074482703684	22.60445502160795
40	22.38849999004242	27.641870631111466	28.523427698006493	21.446201680839625
41	21.282337243343356	27.808348933543108	28.688599067970443	22.22071475514309
42	21.969908191105887	28.831365881345466	26.986101706729333	22.21262422081931
43	21.156622786927688	28.626177483918507	27.458028797323404	22.759170931830404
44	22.736455200844404	27.06800280803776	27.756009399956188	22.43953259116165
45	22.65225140899767	27.741446438173384	27.929395774002746	21.6769063788262
46	20.840220659988447	28.84362615258997	28.587591858682014	21.72856132873957
47	21.762292633381794	29.085782068388667	27.7469853424412	21.40493995578834
48	21.826456794057318	28.567241053113733	27.859319299782925	21.746982853046024
49	20.89610758170195	28.960938900284788	27.77374634058909	22.369207177424173
50	20.364995120785455	28.214182582199825	28.485713361081793	22.935108935932927
51	21.57826409495549	26.59003893414056	29.261720072491187	22.56997689841276
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15847.0
1	12150.5
2	8454.0
3	5266.0
4	2078.0
5	1788.5
6	1499.0
7	1557.5
8	1616.0
9	2128.0
10	2640.0
11	3040.5
12	3441.0
13	3986.5
14	4532.0
15	4984.0
16	5436.0
17	5452.5
18	5469.0
19	5568.0
20	5667.0
21	6905.0
22	8143.0
23	9970.5
24	11798.0
25	12285.0
26	15491.5
27	18211.0
28	22668.0
29	27125.0
30	32530.5
31	37936.0
32	41782.5
33	45629.0
34	49734.5
35	53840.0
36	59774.0
37	65708.0
38	68135.5
39	70563.0
40	74176.0
41	77789.0
42	85069.5
43	92350.0
44	99358.0
45	106366.0
46	114545.0
47	122724.0
48	124425.0
49	126126.0
50	121955.0
51	117784.0
52	106167.0
53	94550.0
54	88658.5
55	82767.0
56	77242.5
57	71718.0
58	68037.5
59	64357.0
60	62499.0
61	60641.0
62	54335.5
63	48030.0
64	43765.0
65	39500.0
66	33666.0
67	27832.0
68	24719.5
69	21607.0
70	18929.0
71	16251.0
72	13013.0
73	9775.0
74	8298.5
75	6248.5
76	5675.0
77	4255.5
78	2836.0
79	2121.0
80	1406.0
81	1149.0
82	892.0
83	580.0
84	268.0
85	217.0
86	166.0
87	125.5
88	85.0
89	64.0
90	43.0
91	31.5
92	20.0
93	23.5
94	27.0
95	15.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1606816.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.1684747525771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.03739378037615	16.63464569519455
2	9.47374033608177	4.200367469058
3	3.659629986778676	2.433852448970314
4	1.9788406469264532	1.7547151568304966
5	1.3330791741191526	1.4776166007323388
6	0.925610980108999	1.2311630185952696
7	0.6771835787493315	1.0508488947855055
8	0.5551377410881418	0.9845245598012125
9	0.4515807427937494	0.9009770665835923
>10	3.956708125489435	19.414840082475635
>50	1.097316708818007	17.479321489573586
>100	0.8450200503724056	29.875474278052238
>500	0.006497980995097536	0.8656798384943675
>1k	0.0019776463898122936	0.8277257086920533
>5k	0.0	0.0
>10k+	2.825209128303277E-4	0.8682476921608607
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13863	0.8627621333120904	No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	3048	0.18969191245295042	Illumina Single End Adapter 2 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2842	0.1768715272937287	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2737	0.17033686495529046	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.244697588273953E-4	0.0	0.0	0.22155617071276362	0.0
2	1.244697588273953E-4	0.0	0.0	0.3366906976281043	0.0
3	1.244697588273953E-4	0.0	0.0	0.4716781510764145	0.0
4	1.244697588273953E-4	0.0	0.0	1.0128726624579292	0.0
5	1.244697588273953E-4	0.0	0.0	1.1079053233226455	0.0
6	1.244697588273953E-4	0.0	0.0	1.6458013860952343	0.0
7	1.244697588273953E-4	0.0	0.0	2.0350183219484994	0.0
8	1.244697588273953E-4	0.0	0.0	2.351420448887738	0.0
9	1.244697588273953E-4	0.0	0.0	2.87388226156573	0.0
10	1.244697588273953E-4	0.0	0.0	3.7352129926513054	0.0
11	1.244697588273953E-4	0.0	0.0	4.495660984207277	0.0
12	1.244697588273953E-4	0.0	0.0	4.849092864397666	0.0
13	1.244697588273953E-4	0.0	0.0	5.003622069981877	0.0
14	1.244697588273953E-4	0.0	0.0	5.174456813972477	0.0
15	1.244697588273953E-4	0.0	0.0	5.322638061856491	0.0
16	1.244697588273953E-4	0.0	0.0	5.6066158166211935	0.0
17	1.244697588273953E-4	0.0	0.0	5.990480552844881	0.0
18	1.244697588273953E-4	0.0	0.0	6.4368291079999205	0.0
19	1.244697588273953E-4	0.0	0.0	6.661932666839265	6.223487941369765E-5
20	1.244697588273953E-4	0.0	0.0	6.896433692470078	6.223487941369765E-5
21	1.244697588273953E-4	0.0	0.0	7.168773524784418	6.223487941369765E-5
22	1.244697588273953E-4	0.0	0.0	7.467065301814271	6.223487941369765E-5
23	1.244697588273953E-4	0.0	0.0	7.763738971979368	6.223487941369765E-5
24	1.244697588273953E-4	0.0	0.0	7.997430944177802	6.223487941369765E-5
25	1.244697588273953E-4	0.0	0.0	8.195711269989843	6.223487941369765E-5
26	1.8670463824109294E-4	0.0	0.0	8.397165574651982	6.223487941369765E-5
27	1.8670463824109294E-4	0.0	0.0	8.604532192858423	6.223487941369765E-5
28	1.8670463824109294E-4	0.0	0.0	8.820549459303368	6.223487941369765E-5
29	1.8670463824109294E-4	0.0	0.0	9.036442255989485	6.223487941369765E-5
30	1.8670463824109294E-4	0.0	0.0	9.299011212235875	6.223487941369765E-5
31	1.8670463824109294E-4	0.0	0.0	9.5172066994603	6.223487941369765E-5
32	1.8670463824109294E-4	0.0	0.0	9.747289048652739	6.223487941369765E-5
33	1.8670463824109294E-4	0.0	0.0	9.969218628641984	6.223487941369765E-5
34	1.8670463824109294E-4	0.0	0.0	10.195193475793122	6.223487941369765E-5
35	1.8670463824109294E-4	0.0	0.0	10.461060880648438	6.223487941369765E-5
36	1.8670463824109294E-4	0.0	0.0	10.690458646167327	6.223487941369765E-5
37	1.8670463824109294E-4	0.0	0.0	10.914753151574294	6.223487941369765E-5
38	1.8670463824109294E-4	0.0	0.0	11.152801565331687	6.223487941369765E-5
39	1.8670463824109294E-4	0.0	0.0	11.393775018421524	6.223487941369765E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTT	35	1.2123382E-7	45.000004	20
CTCCGAT	25	3.891502E-5	45.0	11
TAGGTCG	40	6.8175723E-9	45.0	2
ACCGAAC	40	6.8175723E-9	45.0	25
CGCGAAA	20	7.034125E-4	45.0	20
CCGTCGA	20	7.034125E-4	45.0	30
AATTCGC	20	7.034125E-4	45.0	43
TCGTACA	25	3.891502E-5	45.0	34
CGCACGA	20	7.034125E-4	45.0	40
CGATACG	20	7.034125E-4	45.0	1
GTGCGAT	30	2.1660435E-6	44.999996	15
ACGAACG	85	0.0	42.35294	1
CTCACGA	80	0.0	42.1875	24
TATACGG	145	0.0	41.89655	2
ATAACGG	180	0.0	41.25	2
TACGGGT	290	0.0	41.12069	4
CGAACCG	55	6.184564E-11	40.909092	27
CGTAGAT	55	6.184564E-11	40.909092	21
TCAACCG	110	0.0	40.909092	1
TTCTACG	45	1.9290383E-8	40.0	1
>>END_MODULE