FastQCFastQC Report
Sat 18 Jun 2016
SRR3553302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367651
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270547.35860911571028No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66381.805516644861567No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56421.5346075490070747No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT26780.7284081914641874Illumina Single End Adapter 2 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26490.720520276022641No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7490.20372581605925186No Hit
AAACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT6060.16483023301990202No Hit
TAGTAAGGGATCAGATATTTATCTCTTTTTCTAAAAAAAATAAAATAAATA5710.15531033507320802No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT5400.14687842546327903No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA4940.1343665595904812No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4700.127838629569891No Hit
ACACACGGGATCGGGGTGGGACGAATGGGCCACCATGCCTTGTGGGAGCCA4350.11831873162319699No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4340.11804673453900574No Hit
GCAAACGGGAAAGAATTTTGGTACCCAAAAAAAAAAAAAAAAAAAAAAAAA4300.1169587462022407No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3800.10335889199267784No Hit
AATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT3730.10145491240333904No Hit
AGTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT3690.10036692406657402No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGA253.8856124E-545.00000416
AGGTACC253.8856124E-545.00000419
CAGCGCG502.1827873E-1145.0000041
GTACCTT253.8856124E-545.00000421
CTGTACG253.8856124E-545.0000041
CATTGCG253.8856124E-545.0000041
AACTTAC253.8856124E-545.00000424
GCACGAA253.8856124E-545.00000421
CGATTCT253.8856124E-545.00000418
GAATAGA253.8856124E-545.0000049
CACGACC253.8856124E-545.00000427
CGACACG253.8856124E-545.0000041
CCTCGCG253.8856124E-545.0000041
ACGGGCC253.8856124E-545.0000045
CACCTAG253.8856124E-545.0000041
GTTACGG502.1827873E-1145.0000042
GTTCGTC253.8856124E-545.00000444
GTGCTAG253.8856124E-545.00000431
AAGTTCG253.8856124E-545.00000442
GAGCGTT253.8856124E-545.00000433