##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553302_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367651
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.431496174361012	31.0	31.0	34.0	30.0	34.0
2	31.613478543510013	31.0	31.0	34.0	30.0	34.0
3	31.831310128355423	31.0	31.0	34.0	30.0	34.0
4	35.583855341070745	37.0	35.0	37.0	33.0	37.0
5	35.30620887744083	37.0	35.0	37.0	33.0	37.0
6	34.019872106971015	37.0	35.0	37.0	31.0	37.0
7	34.788761624475384	37.0	35.0	37.0	32.0	37.0
8	35.4093202520869	37.0	35.0	37.0	32.0	37.0
9	36.991883607007736	39.0	37.0	39.0	33.0	39.0
10	36.49693867281743	38.0	35.0	39.0	32.0	39.0
11	36.53004615790519	38.0	35.0	39.0	32.0	39.0
12	36.60670309614281	39.0	35.0	39.0	32.0	39.0
13	36.56854734517246	39.0	35.0	39.0	32.0	39.0
14	37.657803188349824	39.0	37.0	41.0	32.0	41.0
15	37.67282014736802	39.0	37.0	41.0	32.0	41.0
16	37.56455442797653	39.0	36.0	41.0	32.0	41.0
17	37.44203334140258	39.0	36.0	40.0	32.0	41.0
18	37.15596584804611	38.0	36.0	40.0	32.0	41.0
19	36.84049002994688	38.0	35.0	40.0	32.0	41.0
20	36.694911750546034	38.0	35.0	40.0	31.0	41.0
21	36.6485280877789	38.0	35.0	40.0	31.0	41.0
22	36.60552262879742	38.0	35.0	40.0	31.0	41.0
23	36.323575891266444	38.0	35.0	40.0	31.0	41.0
24	36.13439919924058	38.0	35.0	40.0	30.0	41.0
25	36.20431604973194	38.0	35.0	40.0	30.0	41.0
26	36.09181805571044	38.0	35.0	40.0	30.0	41.0
27	36.042515864229934	38.0	35.0	40.0	30.0	41.0
28	35.9146418750391	38.0	35.0	40.0	30.0	41.0
29	35.67804521135533	38.0	34.0	40.0	29.0	41.0
30	35.64356414099241	38.0	34.0	40.0	29.0	41.0
31	35.52436957875812	38.0	34.0	40.0	29.0	41.0
32	35.35162423058825	38.0	34.0	40.0	27.0	41.0
33	35.25539166220138	37.0	34.0	40.0	27.0	41.0
34	35.19390128137827	37.0	34.0	40.0	27.0	41.0
35	34.99139129228535	37.0	33.0	40.0	25.0	41.0
36	34.820005929536435	37.0	33.0	40.0	25.0	41.0
37	34.82588650649665	37.0	33.0	40.0	25.0	41.0
38	34.75159322292065	37.0	33.0	40.0	25.0	41.0
39	34.63363352744859	37.0	33.0	40.0	24.0	41.0
40	34.49613084147738	37.0	33.0	40.0	24.0	41.0
41	34.312649768394486	37.0	33.0	40.0	23.0	41.0
42	34.08238791680153	36.0	33.0	40.0	22.0	41.0
43	33.91640985608634	36.0	33.0	40.0	22.0	41.0
44	33.77685087215865	36.0	33.0	40.0	21.0	41.0
45	33.646863465623646	36.0	33.0	40.0	22.0	41.0
46	33.49150961101697	35.0	33.0	40.0	21.0	41.0
47	33.39383817805473	35.0	33.0	39.0	21.0	41.0
48	33.19560942306698	35.0	32.0	39.0	20.0	41.0
49	32.98909835686561	35.0	32.0	39.0	20.0	41.0
50	32.84554917571283	35.0	31.0	39.0	20.0	41.0
51	31.869612757751238	35.0	30.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	3.0
13	6.0
14	5.0
15	28.0
16	80.0
17	164.0
18	342.0
19	723.0
20	1144.0
21	1787.0
22	2416.0
23	3042.0
24	3689.0
25	4285.0
26	4794.0
27	5650.0
28	6610.0
29	8251.0
30	10094.0
31	12318.0
32	15705.0
33	20409.0
34	28881.0
35	48342.0
36	39340.0
37	45505.0
38	55733.0
39	48291.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.42902372086571	38.17696674291652	18.372043051698487	24.021966484519286
2	19.368912365259444	42.10406064446989	17.17307990458342	21.353947085687242
3	18.01191891222926	42.822948937987384	17.152408126184888	22.01272402359847
4	16.80289187299912	41.89135892463233	17.149144161174593	24.156605041193956
5	14.504788508667188	45.39032941566866	14.898640286576128	25.20624178908802
6	15.883541728432672	48.995378769539585	16.388096319607453	18.732983182420284
7	71.42017837568781	19.979545819268818	4.497743784186634	4.102532020856736
8	72.91915430666583	19.699932816720207	3.7356079542827305	3.645304922331233
9	66.06428379087777	23.642530552072483	6.903829990942498	3.3893556661072592
10	23.114584211657252	52.54031676780425	10.881515350155446	13.463583670383054
11	15.347707472575895	39.28154690181721	28.48979058944488	16.880955036162014
12	19.48315114061977	35.483107621086305	28.791707352897177	16.242033885396747
13	19.622685644809888	37.540221568824784	25.156194325596832	17.680898460768503
14	14.671522721276428	43.38353492850557	25.594109631144757	16.350832719073253
15	13.279713641469765	44.72067259438979	25.34441630785718	16.65519745628327
16	14.491460651541816	41.3571566512807	24.905413013972492	19.245969683204997
17	12.712055726762609	41.122423167623644	26.042632822976135	20.12288828263761
18	14.974527473065487	41.211910208322564	24.787910273601867	19.025652045010077
19	14.375317896592149	45.29241046535981	26.016249105809585	14.316022532238454
20	15.788614746049923	43.61772441799424	23.578339240203345	17.015321595752493
21	16.593998112340238	42.63472695572704	24.224332315157582	16.546942616775148
22	13.325409151613895	41.06938373620635	24.00700664488877	21.59820046729099
23	14.306774631375951	43.605756546289825	22.02849985448156	20.058968967852664
24	12.835542402985439	45.486888380556564	23.125736092109094	18.551833124348907
25	15.004175155242336	43.91039328058403	22.124242828116884	18.96118873605675
26	15.39884292440385	43.27582408316582	22.20611395045845	19.119219041971867
27	12.903269676949064	43.230128573021695	23.450772607717646	20.415829142311594
28	15.582440956232949	42.35919390944129	23.770641178726564	18.287723955599198
29	14.36906196365575	44.44432355685147	23.33354186443121	17.853072615061567
30	15.507369760996163	44.01674414050281	22.373936151404457	18.101949947096568
31	17.000089759037785	42.11412453658497	20.59670720329878	20.28907850107847
32	16.202594308189017	43.588076735817396	21.943364767129697	18.265964188863894
33	15.30146796826338	40.74162724975589	22.1517145336202	21.805190248360535
34	15.93712515401835	40.30996787714436	22.75337208385126	20.999534884986033
35	17.05965712047567	40.69348376585403	22.957913891163088	19.288945222507216
36	17.584339495880606	42.355657947346806	23.115400202909825	16.944602353862766
37	15.981188681657333	43.515453514338326	21.693127449673742	18.8102303543306
38	16.167506684328345	40.71714751217867	24.953556497874345	18.161789305618644
39	16.868443170289215	39.14473236846901	23.094184430342906	20.892640030898868
40	18.806150398067732	39.75400583705743	21.819334096738483	19.620509668136357
41	18.419914538516146	37.14310582590555	25.914250199237866	18.522729436340445
42	20.09704855963944	40.30017598211347	21.988244286021256	17.614531172225835
43	15.48289002341895	40.01158707578655	24.4571618192253	20.048361081569205
44	17.652882761096798	38.23435812768087	22.858090961264896	21.254668149957432
45	17.9577914924752	40.88360972770372	22.846395086644673	18.31220369317641
46	17.037353359571984	38.40816426447908	27.1483009702136	17.406181405735328
47	17.9547995245491	39.90850018087806	22.264049329391188	19.872650965181656
48	17.216599438054022	38.50934717979823	25.909898245890805	18.36415513625694
49	17.01477760158411	38.73864072177146	25.109682824200124	19.1368988524443
50	16.518382922935064	36.01404592942764	25.016115827238334	22.451455320398967
51	17.234007251442264	36.448697269965265	26.963886947131925	19.35340853146054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30374.0
1	23287.0
2	16200.0
3	9642.5
4	3085.0
5	2451.0
6	1817.0
7	1888.5
8	1960.0
9	2152.0
10	2344.0
11	2824.0
12	3304.0
13	3716.5
14	4129.0
15	4304.0
16	4479.0
17	5302.0
18	6125.0
19	5123.5
20	4122.0
21	3983.0
22	3844.0
23	3817.0
24	3790.0
25	3731.5
26	4017.0
27	4361.0
28	4801.5
29	5242.0
30	6635.5
31	8029.0
32	8910.5
33	9792.0
34	10823.0
35	11854.0
36	11803.0
37	11752.0
38	11671.0
39	11590.0
40	12677.5
41	13765.0
42	14642.5
43	15520.0
44	16359.0
45	17198.0
46	20175.5
47	23153.0
48	25354.5
49	27556.0
50	31265.0
51	34974.0
52	30308.5
53	25643.0
54	21924.0
55	18205.0
56	14781.5
57	11358.0
58	9876.0
59	8394.0
60	7206.5
61	6019.0
62	5164.5
63	4310.0
64	3804.5
65	3299.0
66	2950.0
67	2601.0
68	2157.0
69	1713.0
70	1338.5
71	964.0
72	768.5
73	573.0
74	454.0
75	213.0
76	91.0
77	77.0
78	63.0
79	42.5
80	22.0
81	19.5
82	17.0
83	11.5
84	6.0
85	3.5
86	1.0
87	0.5
88	0.0
89	1.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	367651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.145738381186742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02068207999385	21.11512491001691
2	10.321589327601863	5.810175057854623
3	3.967280159469597	3.349860883599122
4	1.9949687958599693	2.245994792276233
5	1.2478092705892463	1.7560256639812188
6	0.9692689288822841	1.6368473816000322
7	0.6997475635388716	1.3786438299366548
8	0.5410313706571569	1.218218193162497
9	0.460176099571332	1.1656796497028683
>10	3.976879747882725	22.656721483904857
>50	0.4861296277420931	9.497381178340918
>100	0.30545311311926177	14.928790861801058
>500	0.003992851151885775	0.6928334512421223
>1k	0.0019964255759428876	1.4966438746012918
>5k	0.0019964255759428876	3.450119538221112
>10k+	9.982127879714438E-4	7.600939249758467
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27054	7.35860911571028	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6638	1.805516644861567	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5642	1.5346075490070747	No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	2678	0.7284081914641874	Illumina Single End Adapter 2 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2649	0.720520276022641	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	749	0.20372581605925186	No Hit
AAACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT	606	0.16483023301990202	No Hit
TAGTAAGGGATCAGATATTTATCTCTTTTTCTAAAAAAAATAAAATAAATA	571	0.15531033507320802	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT	540	0.14687842546327903	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	494	0.1343665595904812	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	470	0.127838629569891	No Hit
ACACACGGGATCGGGGTGGGACGAATGGGCCACCATGCCTTGTGGGAGCCA	435	0.11831873162319699	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	434	0.11804673453900574	No Hit
GCAAACGGGAAAGAATTTTGGTACCCAAAAAAAAAAAAAAAAAAAAAAAAA	430	0.1169587462022407	No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	380	0.10335889199267784	No Hit
AATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT	373	0.10145491240333904	No Hit
AGTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	369	0.10036692406657402	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9071102757778436	0.0
2	0.0	0.0	0.0	1.2103870246510957	0.0
3	0.0	0.0	0.0	1.4856480738526483	0.0
4	0.0	0.0	0.0	2.8981289320578485	0.0
5	0.0	0.0	0.0	3.127150476946887	0.0
6	0.0	0.0	0.0	4.303537866074076	0.0
7	0.0	0.0	0.0	5.214728098114788	0.0
8	0.0	0.0	0.0	5.80768174165173	0.0
9	0.0	0.0	0.0	6.8594944662193225	0.0
10	0.0	0.0	0.0	8.48549303551466	0.0
11	0.0	0.0	0.0	10.110675613557422	0.0
12	0.0	0.0	0.0	10.900283148964643	0.0
13	0.0	0.0	0.0	11.220967711226136	0.0
14	0.0	0.0	0.0	11.530228395951596	0.0
15	0.0	0.0	0.0	11.8101133955844	0.0
16	0.0	0.0	0.0	12.415306907909947	0.0
17	0.0	0.0	0.0	13.256321892229316	0.0
18	0.0	0.0	0.0	14.440053202629668	0.0
19	0.0	0.0	0.0	14.874160548998915	0.0
20	0.0	0.0	0.0	15.371915213068917	0.0
21	0.0	0.0	0.0	15.794870678986321	0.0
22	0.0	0.0	0.0	16.33532888527435	0.0
23	0.0	0.0	0.0	16.92991451131644	0.0
24	0.0	0.0	0.0	17.318870341709935	0.0
25	0.0	0.0	0.0	17.632482979782456	0.0
26	0.0	0.0	0.0	17.89060821267996	0.0
27	0.0	0.0	0.0	18.228428591245503	0.0
28	0.0	0.0	0.0	18.574952876505165	0.0
29	0.0	0.0	0.0	18.90243736587144	0.0
30	0.0	0.0	0.0	19.34361663642966	0.0
31	0.0	0.0	0.0	19.63846147569298	0.0
32	0.0	0.0	0.0	19.94772216041844	0.0
33	0.0	0.0	0.0	20.264054769332873	0.0
34	2.719970841912575E-4	0.0	0.0	20.58745930243628	0.0
35	2.719970841912575E-4	0.0	0.0	20.97097519114595	0.0
36	2.719970841912575E-4	0.0	0.0	21.273163951682438	0.0
37	2.719970841912575E-4	0.0	0.0	21.570728761787674	0.0
38	2.719970841912575E-4	0.0	0.0	21.908549140353216	0.0
39	2.719970841912575E-4	0.0	0.0	22.228417711362134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCGA	25	3.8856124E-5	45.000004	16
AGGTACC	25	3.8856124E-5	45.000004	19
CAGCGCG	50	2.1827873E-11	45.000004	1
GTACCTT	25	3.8856124E-5	45.000004	21
CTGTACG	25	3.8856124E-5	45.000004	1
CATTGCG	25	3.8856124E-5	45.000004	1
AACTTAC	25	3.8856124E-5	45.000004	24
GCACGAA	25	3.8856124E-5	45.000004	21
CGATTCT	25	3.8856124E-5	45.000004	18
GAATAGA	25	3.8856124E-5	45.000004	9
CACGACC	25	3.8856124E-5	45.000004	27
CGACACG	25	3.8856124E-5	45.000004	1
CCTCGCG	25	3.8856124E-5	45.000004	1
ACGGGCC	25	3.8856124E-5	45.000004	5
CACCTAG	25	3.8856124E-5	45.000004	1
GTTACGG	50	2.1827873E-11	45.000004	2
GTTCGTC	25	3.8856124E-5	45.000004	44
GTGCTAG	25	3.8856124E-5	45.000004	31
AAGTTCG	25	3.8856124E-5	45.000004	42
GAGCGTT	25	3.8856124E-5	45.000004	33
>>END_MODULE