##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553300_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 702686 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71865527419075 31.0 31.0 34.0 30.0 34.0 2 31.9197052453016 33.0 31.0 34.0 30.0 34.0 3 32.08128523977993 34.0 31.0 34.0 30.0 34.0 4 35.79298719484948 37.0 35.0 37.0 35.0 37.0 5 35.53988267874983 37.0 35.0 37.0 33.0 37.0 6 34.143991768727425 37.0 35.0 37.0 32.0 37.0 7 34.94747155913168 37.0 35.0 37.0 32.0 37.0 8 35.636282777798336 37.0 35.0 37.0 33.0 37.0 9 37.341734430456846 39.0 37.0 39.0 34.0 39.0 10 36.78974819478402 39.0 37.0 39.0 32.0 39.0 11 36.792873345989534 39.0 37.0 39.0 32.0 39.0 12 36.72777599098317 39.0 35.0 39.0 32.0 39.0 13 36.67770810860043 39.0 35.0 39.0 32.0 39.0 14 37.85875625812952 40.0 37.0 41.0 33.0 41.0 15 37.84707678820981 40.0 37.0 41.0 33.0 41.0 16 37.777876604913146 40.0 37.0 41.0 33.0 41.0 17 37.7546784765884 39.0 37.0 41.0 33.0 41.0 18 37.65014529960751 39.0 36.0 41.0 33.0 41.0 19 37.57313508451855 39.0 36.0 41.0 32.0 41.0 20 37.470244177342366 39.0 36.0 41.0 32.0 41.0 21 37.42868934346209 39.0 36.0 41.0 32.0 41.0 22 37.40941757769473 39.0 35.0 41.0 32.0 41.0 23 37.21363596257788 39.0 35.0 41.0 32.0 41.0 24 37.04460882954833 39.0 35.0 40.0 31.0 41.0 25 37.11999812149381 39.0 35.0 41.0 32.0 41.0 26 37.0362437845638 39.0 35.0 41.0 31.0 41.0 27 36.98435005108968 39.0 35.0 41.0 31.0 41.0 28 36.883233763017905 39.0 35.0 40.0 31.0 41.0 29 36.688032777086775 39.0 35.0 40.0 31.0 41.0 30 36.71132340761023 39.0 35.0 40.0 31.0 41.0 31 36.67650273379575 39.0 35.0 40.0 31.0 41.0 32 36.5642790663255 39.0 35.0 40.0 30.0 41.0 33 36.5176024568584 39.0 35.0 40.0 30.0 41.0 34 36.39155042223696 39.0 35.0 40.0 30.0 41.0 35 36.25591089049732 38.0 35.0 40.0 30.0 41.0 36 36.20650333150226 38.0 35.0 40.0 30.0 41.0 37 36.1695565302283 38.0 35.0 40.0 30.0 41.0 38 36.04547550399467 38.0 35.0 40.0 30.0 41.0 39 36.00098336952778 38.0 35.0 40.0 30.0 41.0 40 35.80711583836877 38.0 35.0 40.0 29.0 41.0 41 35.68018432130425 38.0 35.0 40.0 29.0 41.0 42 35.54922255459764 38.0 34.0 40.0 28.0 41.0 43 35.49490241729592 38.0 34.0 40.0 28.0 41.0 44 35.34157077272067 38.0 34.0 40.0 27.0 41.0 45 35.148142982783206 38.0 34.0 40.0 26.0 41.0 46 35.00646519213418 37.0 34.0 40.0 26.0 41.0 47 34.90761165015384 37.0 34.0 40.0 26.0 41.0 48 34.74312139419314 37.0 33.0 40.0 26.0 41.0 49 34.538246101388104 37.0 33.0 40.0 25.0 41.0 50 34.45229021212889 37.0 33.0 40.0 25.0 41.0 51 33.250343681245965 35.0 31.0 39.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 6.0 10 3.0 11 13.0 12 8.0 13 25.0 14 24.0 15 54.0 16 103.0 17 240.0 18 449.0 19 771.0 20 1206.0 21 1830.0 22 2529.0 23 3317.0 24 4179.0 25 5025.0 26 6094.0 27 7741.0 28 9530.0 29 11834.0 30 14773.0 31 19069.0 32 25246.0 33 33418.0 34 49706.0 35 67175.0 36 70986.0 37 96390.0 38 134468.0 39 136449.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.55028846454889 31.79200951776469 20.52865718115915 27.129044836527267 2 24.80866275975329 31.806809869557668 19.27233501165528 24.112192359033767 3 21.03756158511768 33.44438340880564 20.149113544314247 25.36894146176244 4 20.429324050856284 35.05634095456577 17.6969514121528 26.81738358242515 5 18.473969881284102 36.63969397426447 15.132078908644827 29.7542572358066 6 18.54740239594926 42.58317370774428 17.478646223206383 21.390777673100075 7 83.1768385879326 9.328633272898564 4.1014051795538835 3.393122959614963 8 83.96723429810756 8.986517448760898 3.747050602972025 3.2991976501595306 9 77.23250498800319 11.672354365961468 6.729321489256937 4.365819156778419 10 34.96241564511033 34.29341128185278 13.056044947529907 17.68812812550698 11 26.15122544066624 32.72002003739935 22.000438318110792 19.128316203823616 12 26.197049606794497 26.158767927637665 28.77046077479842 18.87372169076942 13 23.657793096774377 27.695585225833447 27.13601807919896 21.510603598193217 14 20.50204501014678 31.284955157780285 26.908178048232067 21.304821783840865 15 19.448943055646474 32.4147627816692 27.655738124852352 20.480556037831978 16 21.103736234961275 30.325636201660483 26.90476258243369 21.665864980944548 17 21.317060536285055 30.418992266816186 27.311772256740564 20.952174940158194 18 22.120833487503663 30.184178993177607 25.819498324998648 21.87548919432008 19 20.78012085056483 33.136991486951494 25.525056710963362 20.55783095152031 20 22.890736402888344 31.024098957429064 25.677756494365905 20.407408145316687 21 23.364632282413485 30.210079608815317 26.40240448792206 20.022883620849143 22 22.23155150380113 28.73289065101624 24.63319320436155 24.402364640821077 23 21.061185223556468 32.74563603088719 24.543679538229025 21.649499207327313 24 19.49362873317527 32.848811560213235 25.988279259868563 21.669280446742924 25 22.257736741588705 31.45999777994723 23.898298813410257 22.38396666505381 26 21.798641213856545 31.38813068710633 22.620203049441713 24.19302504959541 27 20.27263955735563 30.28664296712899 26.15449859538969 23.286218880125688 28 20.4705942625867 32.092285885872215 26.402973732221792 21.034146119319296 29 21.18670359164691 33.5669132443225 24.103653694537815 21.142729469492775 30 21.332999376677492 31.597327967257065 25.969636509052407 21.100036147013032 31 23.322650515308403 31.497710214804336 21.281909700776733 23.897729569110528 32 22.650799930552196 31.409477348346204 24.22120264243204 21.718520078669563 33 22.618068383317727 30.923058094227006 23.030912811696833 23.427960710758434 34 20.562669528068014 31.849930125262212 24.437230854179536 23.150169492490242 35 21.91391318455185 30.614527683773407 24.386141178278777 23.08541795339597 36 23.242956313346216 31.506106568225352 24.761130860725842 20.489806257702586 37 23.129249764475173 31.14278639392275 23.42326444528566 22.304699396316423 38 22.434344785579903 31.849076258812616 23.92604947302209 21.790529482585395 39 21.94977557543483 28.65504649302818 25.179525421027314 24.21565251050967 40 22.371300979384817 30.906550009534843 25.133558943824124 21.588590067256213 41 20.84501470073404 30.605135152827863 27.205892816990808 21.343957329447292 42 20.584585433607614 31.905146822336008 24.733522512188944 22.776745231867434 43 21.04055011769126 29.33059716573263 25.264058199537203 24.364794517038906 44 21.779856151965458 28.200789541843722 26.657852867425845 23.361501438764968 45 22.961322696054854 29.39961803707488 25.615993487845213 22.023065779025057 46 21.554720031422285 28.880324924646285 27.536196821909076 22.028758222022354 47 21.14827960141514 30.333178688631907 26.815248916301165 21.703292793651787 48 21.06773153300336 30.60157737595455 26.751493554731415 21.57919753631067 49 21.301975562342214 30.512063709822023 26.27005518823486 21.915905539600903 50 20.712807712121773 29.019789778080106 26.807279496104947 23.46012301369317 51 22.407447992417666 28.398317313849997 26.438551500954908 22.75568319277743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18038.0 1 13608.0 2 9178.0 3 5765.5 4 2353.0 5 1965.0 6 1577.0 7 1537.5 8 1498.0 9 1519.5 10 1541.0 11 1687.0 12 1833.0 13 1851.5 14 1870.0 15 1996.0 16 2122.0 17 2119.5 18 2117.0 19 2164.0 20 2211.0 21 2701.0 22 3191.0 23 3807.5 24 4424.0 25 4940.0 26 5361.0 27 5266.0 28 8520.0 29 11774.0 30 13107.0 31 14440.0 32 16351.0 33 18262.0 34 18243.5 35 18225.0 36 21284.5 37 24344.0 38 26849.0 39 29354.0 40 29194.5 41 29035.0 42 32466.0 43 35897.0 44 36473.0 45 37049.0 46 41726.5 47 46404.0 48 50059.0 49 53714.0 50 53670.5 51 53627.0 52 50158.0 53 46689.0 54 41343.5 55 35998.0 56 34906.5 57 33815.0 58 32979.5 59 32144.0 60 30028.5 61 27913.0 62 24891.5 63 21870.0 64 20639.0 65 19408.0 66 17522.0 67 15636.0 68 13479.5 69 11323.0 70 10056.0 71 8789.0 72 7702.5 73 6616.0 74 4978.5 75 2693.5 76 2046.0 77 1618.0 78 1190.0 79 946.5 80 703.0 81 441.0 82 179.0 83 130.0 84 81.0 85 64.5 86 48.0 87 39.5 88 31.0 89 25.5 90 20.0 91 25.0 92 30.0 93 18.5 94 7.0 95 7.5 96 8.0 97 4.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 702686.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.082927567419528 #Duplication Level Percentage of deduplicated Percentage of total 1 77.45523705073738 16.329831524580058 2 9.299281808930623 3.921121696134127 3 3.351568911552731 2.1198265379844403 4 1.768121095362718 1.4910867593583466 5 1.0978375666331703 1.1572814949059622 6 0.7620778594005199 0.964009938628517 7 0.533135369622879 0.7868038066972017 8 0.3771818802559324 0.6361678608943144 9 0.3013752133799293 0.571848461487419 >10 2.949763665642111 14.260995581646165 >50 1.0573225676143667 16.192708454186437 >100 1.0368714541208588 36.86727852088552 >500 0.006135334048052418 0.7855905456001195 >1k 0.0034085189155846772 1.6273768290944297 >5k 0.0 0.0 >10k+ 6.817037831169354E-4 2.288071987916923 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15920 2.2655923129249764 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4027 0.5730866987530703 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2547 0.36246630785300976 No Hit CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT 2194 0.31223049840184663 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1533 0.21816287787148173 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 1022 0.14544191858098782 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 986 0.14031871988341876 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.423110749324734E-4 0.0 0.0 0.3870861238163276 0.0 2 1.423110749324734E-4 0.0 0.0 0.5158776466302161 0.0 3 1.423110749324734E-4 0.0 0.0 0.6375536156974808 0.0 4 1.423110749324734E-4 0.0 0.0 1.2486373714575216 0.0 5 1.423110749324734E-4 0.0 0.0 1.3364433046908577 0.0 6 1.423110749324734E-4 0.0 0.0 1.8792177444833111 0.0 7 1.423110749324734E-4 0.0 0.0 2.298750793384243 0.0 8 1.423110749324734E-4 0.0 0.0 2.6215123113310925 0.0 9 1.423110749324734E-4 0.0 0.0 3.124866583367251 0.0 10 1.423110749324734E-4 0.0 0.0 3.9300626453351852 0.0 11 1.423110749324734E-4 0.0 0.0 4.66965330175925 0.0 12 1.423110749324734E-4 0.0 0.0 5.025004055865636 0.0 13 1.423110749324734E-4 0.0 0.0 5.156072555878444 0.0 14 1.423110749324734E-4 0.0 0.0 5.3269881568723445 0.0 15 1.423110749324734E-4 0.0 0.0 5.45677585721076 0.0 16 1.423110749324734E-4 0.0 0.0 5.771283332811526 0.0 17 1.423110749324734E-4 0.0 0.0 6.1606464338267735 0.0 18 1.423110749324734E-4 0.0 0.0 6.674816347557799 0.0 19 1.423110749324734E-4 0.0 0.0 6.881309717284818 0.0 20 1.423110749324734E-4 0.0 0.0 7.111284414375696 0.0 21 1.423110749324734E-4 0.0 0.0 7.370290570752797 0.0 22 1.423110749324734E-4 0.0 0.0 7.6396854355999695 0.0 23 1.423110749324734E-4 0.0 0.0 7.927296118038498 0.0 24 1.423110749324734E-4 0.0 0.0 8.12880860014288 0.0 25 1.423110749324734E-4 0.0 0.0 8.319505440552394 0.0 26 1.423110749324734E-4 0.0 0.0 8.497394284217986 0.0 27 1.423110749324734E-4 0.0 0.0 8.668025263062022 0.0 28 1.423110749324734E-4 0.0 0.0 8.860287525295794 0.0 29 1.423110749324734E-4 0.0 0.0 9.045718855932806 0.0 30 1.423110749324734E-4 0.0 0.0 9.279820574196725 0.0 31 1.423110749324734E-4 0.0 0.0 9.469948170306509 0.0 32 1.423110749324734E-4 0.0 0.0 9.667618253387715 0.0 33 1.423110749324734E-4 0.0 0.0 9.886208064483995 0.0 34 1.423110749324734E-4 0.0 0.0 10.089570590562499 0.0 35 1.423110749324734E-4 0.0 0.0 10.32723008569973 0.0 36 1.423110749324734E-4 0.0 0.0 10.53030798962837 0.0 37 1.423110749324734E-4 0.0 0.0 10.737939847954847 0.0 38 1.423110749324734E-4 0.0 0.0 10.961795168823627 0.0 39 1.423110749324734E-4 0.0 0.0 11.170707826824499 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCATC 20 7.0314115E-4 45.000004 37 ACTATCG 40 6.8084773E-9 45.000004 34 GCCCATA 20 7.0314115E-4 45.000004 9 GATCGCG 20 7.0314115E-4 45.000004 9 TAGACTA 20 7.0314115E-4 45.000004 44 ACACCGT 20 7.0314115E-4 45.000004 36 CGTGATC 55 1.8189894E-12 45.000004 39 CGGTTGA 20 7.0314115E-4 45.000004 17 CGCATGA 20 7.0314115E-4 45.000004 32 TAGCGGA 20 7.0314115E-4 45.000004 10 TAGCGAG 40 6.8084773E-9 45.000004 1 CGATTTC 20 7.0314115E-4 45.000004 30 CAATTGC 20 7.0314115E-4 45.000004 40 CGATTAT 20 7.0314115E-4 45.000004 36 CGACACT 20 7.0314115E-4 45.000004 17 TAACGCG 20 7.0314115E-4 45.000004 1 ATCGTCG 20 7.0314115E-4 45.000004 22 ATCGTCC 20 7.0314115E-4 45.000004 26 TACGCTT 20 7.0314115E-4 45.000004 14 CTAACGC 20 7.0314115E-4 45.000004 35 >>END_MODULE