##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553299_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	697282
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.702836155242785	31.0	31.0	34.0	30.0	34.0
2	31.904319916475686	33.0	31.0	34.0	30.0	34.0
3	32.058449809402795	33.0	31.0	34.0	30.0	34.0
4	35.78482450428951	37.0	35.0	37.0	35.0	37.0
5	35.55128484601639	37.0	35.0	37.0	33.0	37.0
6	32.67948692207744	37.0	35.0	37.0	26.0	37.0
7	34.21428632891714	37.0	35.0	37.0	27.0	37.0
8	35.3308962514449	37.0	35.0	37.0	32.0	37.0
9	37.20804638582381	39.0	37.0	39.0	34.0	39.0
10	36.740219882343155	39.0	37.0	39.0	32.0	39.0
11	36.790519474186915	39.0	37.0	39.0	32.0	39.0
12	36.73352244859325	39.0	35.0	39.0	32.0	39.0
13	36.70525985182466	39.0	35.0	39.0	32.0	39.0
14	37.85826824728015	40.0	37.0	41.0	33.0	41.0
15	37.87768076617495	40.0	37.0	41.0	33.0	41.0
16	37.839334731141776	40.0	37.0	41.0	33.0	41.0
17	37.835524221190276	39.0	37.0	41.0	33.0	41.0
18	37.72771561577669	39.0	37.0	41.0	33.0	41.0
19	37.65424032170628	39.0	36.0	41.0	33.0	41.0
20	37.5377408279577	39.0	36.0	41.0	32.0	41.0
21	37.48798190688989	39.0	36.0	41.0	32.0	41.0
22	37.43173350237063	39.0	36.0	41.0	32.0	41.0
23	37.24689150157325	39.0	35.0	41.0	32.0	41.0
24	37.13497408509039	39.0	35.0	41.0	32.0	41.0
25	37.17988848127443	39.0	35.0	41.0	32.0	41.0
26	37.104356630459414	39.0	35.0	41.0	32.0	41.0
27	37.023048063767604	39.0	35.0	41.0	31.0	41.0
28	36.96208277282362	39.0	35.0	41.0	31.0	41.0
29	36.73953006100832	39.0	35.0	40.0	31.0	41.0
30	36.765106513577	39.0	35.0	40.0	31.0	41.0
31	36.43643748153545	39.0	35.0	40.0	30.0	41.0
32	36.49850562613118	39.0	35.0	40.0	30.0	41.0
33	36.49707005200192	39.0	35.0	40.0	30.0	41.0
34	36.41450804695948	39.0	35.0	40.0	30.0	41.0
35	36.371674587899875	39.0	35.0	40.0	30.0	41.0
36	36.28595460660106	38.0	35.0	40.0	30.0	41.0
37	36.23505697838177	38.0	35.0	40.0	30.0	41.0
38	36.10681474640103	38.0	35.0	40.0	30.0	41.0
39	36.03599978201072	38.0	35.0	40.0	30.0	41.0
40	35.86495133963017	38.0	35.0	40.0	29.0	41.0
41	35.79262335755118	38.0	35.0	40.0	29.0	41.0
42	35.6608158535571	38.0	35.0	40.0	29.0	41.0
43	35.627677180824975	38.0	35.0	40.0	28.0	41.0
44	35.421571186406645	38.0	34.0	40.0	28.0	41.0
45	35.22242650749625	38.0	34.0	40.0	27.0	41.0
46	34.98540475732917	37.0	34.0	40.0	26.0	41.0
47	34.94378027828052	37.0	34.0	40.0	26.0	41.0
48	34.87958960650067	37.0	34.0	40.0	26.0	41.0
49	34.721571760062645	37.0	33.0	40.0	26.0	41.0
50	34.61567486325475	37.0	33.0	40.0	26.0	41.0
51	33.47678270771367	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	10.0
11	15.0
12	16.0
13	24.0
14	36.0
15	56.0
16	111.0
17	209.0
18	394.0
19	769.0
20	1183.0
21	1790.0
22	2427.0
23	3191.0
24	3968.0
25	4996.0
26	6014.0
27	7543.0
28	9116.0
29	11592.0
30	14695.0
31	18984.0
32	25099.0
33	33658.0
34	49999.0
35	67254.0
36	72465.0
37	98484.0
38	131493.0
39	131663.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.569726452138447	31.54849831201723	20.651472431526983	27.230302804317336
2	24.922054491583033	31.705393226843658	19.231673842147078	24.14087843942623
3	21.0197595807722	33.43984212986998	20.224528956720523	25.3158693326373
4	20.366795643656367	35.03905163190789	17.779893931006395	26.814258793429346
5	18.450641203989203	36.6745448756744	15.045992869455974	29.828821050880418
6	17.680938271746584	45.01392549929584	16.747886794725808	20.557249434231775
7	83.26229559919803	9.299967588436242	4.062631761611515	3.3751050507542146
8	83.84656423082771	9.02748099047444	3.7359346720552087	3.390020106642649
9	77.26873775602984	11.625138753043963	6.707329315829176	4.39879417509702
10	35.07146319566545	34.16078430247733	13.071038690228631	17.69671381162858
11	26.186535720124716	32.713737053301244	21.95252422979512	19.147202996778923
12	26.21823021388764	26.023330589345488	28.83367131232414	18.924767884442737
13	23.60795201941252	27.801377348045698	27.074842029480184	21.5158286030616
14	20.554381154253228	31.25206157623458	26.830321161309197	21.363236108202994
15	19.487094174236535	32.420312011496065	27.60733820749711	20.485255606770288
16	21.097920210187556	30.30093993534897	26.88739993288225	21.713739921581226
17	21.356639064252338	30.326754455155875	27.423194632874505	20.89341184771728
18	22.165494018202107	30.124110474671657	25.876618068442898	21.833777438683345
19	20.883516281791298	32.98937875923944	25.581902300647368	20.545202658321884
20	22.84785782509802	31.114527551263333	25.559673130813643	20.477941492824996
21	23.491930094280363	30.20972863203123	26.340132112975812	19.958209160712595
22	22.33931178490195	28.723099119151218	24.620885093835778	24.316704002111052
23	20.93471507940833	32.851557906270344	24.69373940529083	21.51998760903049
24	19.610717041311837	32.81513075054282	26.008845775453832	21.565306432691507
25	22.242650749624975	31.393611193175786	24.094699131771648	22.26903892542759
26	21.860882684480597	31.43849977484002	22.60634865090451	24.09426888977487
27	20.311724668068297	30.25189234771585	26.1601475443221	23.276235439893757
28	20.473782486856106	32.00885724857375	26.511511841693892	21.00584842287625
29	21.221686491261785	33.6024736046535	24.19379820503039	20.982041699054328
30	21.286222790779053	31.533870084126654	26.02060572336587	21.159301401728424
31	23.34335319139172	31.430898832896876	21.184972507536408	24.040775468175
32	22.581107787093313	31.457717250696277	24.420965979331175	21.540208982879236
33	22.657117206524763	30.85537845520177	23.11632883108986	23.371175507183608
34	20.676856709337113	31.718300486747115	24.581589658129708	23.023253145786065
35	21.96342369371359	30.541301797551064	24.435163965224973	23.060110543510373
36	23.230486374235962	31.56298312590889	24.848052868136563	20.35847763171859
37	23.109014717144568	31.061607785659174	23.576114111650668	22.25326338554559
38	22.398828594456763	31.86056717368296	23.910698971147973	21.82990526071231
39	21.79534248697084	28.710478687245622	25.20314592948047	24.291032896303076
40	22.437120132170342	30.844622405282223	25.10462051221744	21.613636950329994
41	20.878353377829917	30.551771019472753	27.174658172733558	21.395217429963772
42	20.506767706609377	31.995089504676734	24.805602324454096	22.692540464259796
43	21.105951394127484	29.488929873422805	25.124555058068328	24.280563674381384
44	21.83764961665438	28.215413562948704	26.640584440728425	23.306352379668485
45	22.909095602639965	29.347236842482666	25.831155830782954	21.912511724094415
46	21.570756164650746	28.957437593398367	27.45861789060954	22.013188351341352
47	21.16647210167479	30.210015460029084	26.99725505606053	21.626257382235593
48	21.11297868007492	30.601392263101584	26.824010945356395	21.461618111467097
49	21.2196786952768	30.625055572924587	26.346585742927537	21.808679988871074
50	20.937439945387947	29.01193491299073	26.823724117358545	23.22690102426278
51	22.512699309605008	28.441290611259145	26.42030053837615	22.625709540759694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17718.0
1	13454.5
2	9191.0
3	5774.0
4	2357.0
5	1955.5
6	1554.0
7	1537.5
8	1521.0
9	1566.0
10	1611.0
11	1730.5
12	1850.0
13	1850.0
14	1850.0
15	1968.0
16	2086.0
17	2049.5
18	2013.0
19	2083.5
20	2154.0
21	2623.5
22	3093.0
23	3790.5
24	4488.0
25	4986.5
26	5473.5
27	5462.0
28	8509.0
29	11556.0
30	13183.0
31	14810.0
32	16636.5
33	18463.0
34	18363.0
35	18263.0
36	21258.5
37	24254.0
38	26559.0
39	28864.0
40	28955.5
41	29047.0
42	32240.5
43	35434.0
44	35963.5
45	36493.0
46	41323.0
47	46153.0
48	49860.5
49	53568.0
50	53225.5
51	52883.0
52	49490.5
53	46098.0
54	40942.5
55	35787.0
56	34670.5
57	33554.0
58	32749.5
59	31945.0
60	29720.5
61	27496.0
62	24614.0
63	21732.0
64	20492.5
65	19253.0
66	17246.0
67	15239.0
68	13329.5
69	11420.0
70	10063.0
71	8706.0
72	7600.0
73	6494.0
74	4855.0
75	2614.5
76	2013.0
77	1589.5
78	1166.0
79	891.0
80	616.0
81	373.0
82	130.0
83	96.5
84	63.0
85	56.0
86	49.0
87	38.5
88	28.0
89	27.0
90	26.0
91	19.5
92	13.0
93	12.0
94	11.0
95	7.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	697282.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.337299906855055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.33531130210076	16.501267306429213
2	9.02360987323782	3.850789402154673
3	3.3158099326101707	2.1225129289869615
4	1.7011584450810937	1.4519251172709802
5	1.096659861524759	1.1699880180581956
6	0.7543272530524298	0.9657184095776303
7	0.5672298690556483	0.8472207682516543
8	0.43323098959621154	0.7395183643166374
9	0.3874614973953203	0.7440643955054779
>10	3.3495961819992472	15.854918359171059
>50	1.0450345683350903	16.22424006595163
>100	0.9803581618257525	34.857704316399285
>500	0.006808042790456615	0.9562811460076784
>1k	0.002723217116182646	1.4516356512311606
>5k	0.0	0.0
>10k+	6.808042790456615E-4	2.262215750687768
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15573	2.2333862052942712	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3886	0.5573067998313451	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2534	0.3634110732816852	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	2047	0.29356845580410795	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1526	0.2188497623630038	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	997	0.14298375693048151	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	980	0.14054571894871803	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4341399892726329E-4	0.0	0.0	0.35853499731815824	0.0
2	1.4341399892726329E-4	0.0	0.0	0.4788593424181321	0.0
3	1.4341399892726329E-4	0.0	0.0	0.5922998155695974	0.0
4	1.4341399892726329E-4	0.0	0.0	1.138993979480325	0.0
5	1.4341399892726329E-4	0.0	0.0	1.2261896908281011	0.0
6	1.4341399892726329E-4	0.0	0.0	1.7562478308632663	0.0
7	1.4341399892726329E-4	0.0	0.0	2.178171815707275	0.0
8	1.4341399892726329E-4	0.0	0.0	2.5005664852957628	0.0
9	1.4341399892726329E-4	0.0	0.0	3.0121242194693107	0.0
10	1.4341399892726329E-4	0.0	0.0	3.7996104875789136	0.0
11	1.4341399892726329E-4	0.0	0.0	4.50305615231714	0.0
12	1.4341399892726329E-4	0.0	0.0	4.855424347681426	0.0
13	1.4341399892726329E-4	0.0	0.0	4.993818856646235	0.0
14	1.4341399892726329E-4	0.0	0.0	5.155446433437261	0.0
15	1.4341399892726329E-4	0.0	0.0	5.288391210442834	0.0
16	1.4341399892726329E-4	0.0	0.0	5.599025932119286	0.0
17	1.4341399892726329E-4	0.0	0.0	5.967456495363425	0.0
18	1.4341399892726329E-4	0.0	0.0	6.469118663610993	0.0
19	1.4341399892726329E-4	0.0	0.0	6.6683207081209614	0.0
20	1.4341399892726329E-4	0.0	0.0	6.883441706511856	0.0
21	1.4341399892726329E-4	0.0	0.0	7.1349898606302755	0.0
22	1.4341399892726329E-4	0.0	0.0	7.387541912741186	0.0
23	1.4341399892726329E-4	0.0	0.0	7.6726489426085855	0.0
24	1.4341399892726329E-4	0.0	0.0	7.878448031069209	0.0
25	2.8682799785452657E-4	0.0	0.0	8.062017949696106	0.0
26	2.8682799785452657E-4	0.0	0.0	8.229095258446367	0.0
27	2.8682799785452657E-4	0.0	0.0	8.406211547121538	0.0
28	2.8682799785452657E-4	0.0	0.0	8.595087783708744	0.0
29	2.8682799785452657E-4	0.0	0.0	8.790274236248749	0.0
30	2.8682799785452657E-4	0.0	0.0	9.010988380597807	0.0
31	2.8682799785452657E-4	0.0	0.0	9.195705611216122	0.0
32	2.8682799785452657E-4	0.0	0.0	9.38085308383122	0.0
33	2.8682799785452657E-4	0.0	0.0	9.608164272130931	0.0
34	2.8682799785452657E-4	0.0	0.0	9.805501934654846	0.0
35	2.8682799785452657E-4	0.0	0.0	10.035681402933102	0.0
36	2.8682799785452657E-4	0.0	0.0	10.231728339466672	0.0
37	2.8682799785452657E-4	0.0	0.0	10.435089389945531	0.0
38	2.8682799785452657E-4	0.0	0.0	10.65107087232999	0.0
39	2.8682799785452657E-4	0.0	0.0	10.862463106748775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCT	25	3.889222E-5	45.0	10
GTTTGCG	20	7.031376E-4	45.0	1
CTTCGAC	25	3.889222E-5	45.0	36
CGGAACC	25	3.889222E-5	45.0	44
TCGTTGC	25	3.889222E-5	45.0	13
GGTACGC	20	7.031376E-4	45.0	18
CTCCGTA	45	3.8380676E-10	45.0	18
CACGGTT	20	7.031376E-4	45.0	37
TTTACGT	25	3.889222E-5	45.0	10
CGTGATC	20	7.031376E-4	45.0	39
GACGTAT	25	3.889222E-5	45.0	26
TAAGTAG	20	7.031376E-4	45.0	43
TAAGTAA	25	3.889222E-5	45.0	35
TGACGTA	25	3.889222E-5	45.0	25
GCGCATT	20	7.031376E-4	45.0	13
CCGTCGG	25	3.889222E-5	45.0	2
ATCGTCA	40	6.8084773E-9	45.0	21
ATACTCG	25	3.889222E-5	45.0	44
CATTACG	25	3.889222E-5	45.0	1
GGCCATA	40	6.8084773E-9	45.0	32
>>END_MODULE